Zhang S.-Q.,Nankai Hospital of Tianjin
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Hyperbaric oxygen therapy can improve the microenvironment of the injured spinal cord, and hyperbaric oxygen combined with Schwann cell transplantation is expected to improve the therapeutic efficacy on spinal cord injury. OBJECTIVE: To investigate the effect of Schwann cell transplantation plus hyperbaric oxygen on the neural functional recovery of rats with spinal cord injury. METHODS: A total of 80 female SD rats with spinal cord injury were randomized into 4 groups, with 20 in each group: blank control group, injection of L-DMEM via the tail vein at 6 hours after modeling; cell transplantation group, injection of 3×106 Schwann cell suspension via the tail vein at 6 hours after modeling; hyperbaric oxygen group, hyperbaric oxygen therapy at 1 hour after modeling; combination group, combined therapy of Schwann cell transplantation and hyperbaric oxygen. Inclined plane test, modified Tarlov score, Basso-Beattie-Bresnahan score for motor function evaluation of rat hind limbs were performed and measured at 1, 3 days, 1, 2, 3, 4 weeks after treatment. SRY gene expression in the spinal cord was measured at 4 weeks after transplantation using PCR method. Horseradish peroxidase tracer and electroneurophysiology detection was done at 8 weeks after transplantation. RESULTS AND CONCLUSION: The motor function of the lower limbs was better in the combination group than the cell transplantation and hyperbaric oxygen groups, as well as better in the cell transplantation group and hyperbaric oxygen groups than the blank control group. SRY expression was detected in the cell transplantation group and combination group, but not in the blank control group and hyperbaric oxygen group. The number of nerve fibers positive for horseradish peroxidase was higher in the combination group than the cell transplantation and hyperbaric oxygen groups followed by the blank control group, and there were significant differences between different groups (P < 0.01). In addition, the latencies and amplitudes of somatosensory evoked potential and motor evoked potential in the combination group were also better than those in the other groups (P < 0.05 or P < 0.01). These findings indicate that the combined therapy of Schwann cell transplantation and hyperbaric oxygen can promote the synaptic regeneration, improve limb motor function and electrophysiological function in rats with spinal cord injury, which is superior to hyperbaric oxygen or Schwann cell transplantation alone. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
Tian D.,Nankai Hospital of Tianjin |
Chen Y.,Nankai Hospital of Tianjin |
Zhang Y.,Hebei Medical University |
Wang Y.,Hebei Medical University |
Li W.,Hebei Medical University
Chinese Journal of Clinical Oncology | Year: 2010
Objective: To investigate the efficacy of replication-deficient adenovirus carrying the HSV-TK gene under the control of the human telomerase reverse transcriptase (hTERT) promoter and ganciclovir (GCV) in a mouse xenograft model of renal carcinoma. Methods: Researchers established the model of human renal carcinoma cell line 786-0 xenografts in BALB/C nude mice and administered peritoneal injections of GCV and tail vein injections of Ad-hTERT-HSV-TK at the dose of 1.0×109 pfu/kg. Then measured tumor size and tumor weight every 5 days. After 30 days, all nude mice were killed and all tumors were completely resected. Then the antitumor efficacy was evaluated by relative tumor volume, tumor weight, tumor proliferation and tumor apoptosis. All these factors were compared with those of the negative control group and single therapy group. Results: This study has established the model of human renal carcinoma cell line 786-0 xenografts in BALB/C nude mice successfully. The Ad-hTERT-HSV-TK GCV system could suppress tumor growth in nude mice more effectively while having no significant damage to heart, lung, liver, brain and spleen. Ad-TK or GCV alone caused inhibition of tumor growth, but this was not statistically significant as compared to the negative control group (P>0.05). The tumor volume and weight were significantly smaller than the negative group and single therapy group (P<0.05). The proliferation index of the Ad-hTERT-HSV-TK/GCV group was 3.2±0.3(%), while other groups were 0.1±0.1(%), 0.2±0.1(%), 0.2±0.1(%), respectively, the difference was statistically significant (P<0.05). The apoptosis index of the Ad-hTERT-HSV-TK/GCV group was 27.57±9.36(%), while other groups were 71.2±13.1(%), 69.8±11.6(%), 72.4±12.3(%), respectively, the difference was statistically significant (P<0.01). Conclusion: Significant inhibition of tumor proliferation, promotion of apoptosis and prolonged mice survival time were observed in renal carcinoma xenografts in vivo. This system did not damage normal tissue. These findings indicate that Ad-hTERT-HSV-TK/GCV system is safe, effective and highly specific. The results presented here are encouraging and will lead to a further clinical evaluation of the potential therapeutic benefits of this strategy.
Qian D.,Nankai Hospital of Tianjin |
Hao F.,Nankai Hospital of Tianjin |
Qin M.-F.,Nankai Hospital of Tianjin
World Chinese Journal of Digestology | Year: 2014
AIM: To evaluate the clinical application of pancreatic duct stents in the prevention of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis. METHODS: From January 2012 to December 2012, we performed pancreatic duct stenting in 141 patients to prevent the occurrence of post-ERCP pancreatitis, and 139 patients who did not undergo pancreatic duct stenting in the same period were used as controls. The same medications were given after ERCP in the two groups. The pancreatic duct stents were removed at the end or 24, 48, 72, 96 h after ERCP when the patient's condition was stable. The incidence of post-ERCP pancreatitis was compared between the two groups. RESULTS: The incidence of PEP in the pancreatic duct stenting group was significantly lower than that in the control group (2.1% vs 10.8%, P < 0.05). CONCLUSION: Pancreatic duct stenting may, to a certain extent, decrease the incidence of post- ERCP pancreatitis and reduce its severity. © 2014 Baishideng Publishing Group Co., Limited. All rights reserved.