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Song J.,Tianjin Medical University | Chen S.,Tianjin Medical University | Chen S.,Tianjin Key Laboratory of Ophthalmology and Visual Science | Chen S.,Tianjin Eye Institute | And 4 more authors.
PLoS ONE | Year: 2015

Objectives: To date, the relationship between C-reactive protein (CRP) level and diabetic retinopathy (DR) remains controversial. Therefore, a systematic review and meta-analysis was used to reveal the potential relationship between CRP level and DR. Methods: A systematic search of PubMed, Embase.com, and Web of Science was performed to identify all comparative studies that compared the CRP level of two groups (case group and control group). We defined that diabetic patients without retinopathy and /or matched healthy persons constituted the control group, and patients with DR were the case group. Results: Two cross sectional studies and twenty case control studies including a total of 3679 participants were identified. After pooling the data from all 22 studies, obvious heterogeneity existed between the studies, so a subgroup analysis and sensitivity analysis were performed. Removing the sensitivity studies, the blood CRP levels in the case group were observed to be higher than those in the control group [SMD = 0.22, 95% confidence interval (CI), 0.11-0.34], and the blood CRP levels in the proliferative diabetic retinopathy (PDR) group were also higher than those in the non-proliferative diabetic retinopathy (NPDR) group [SMD = 0.50, 95% CI, 0.30-0.70]. Conclusions: The results from this current meta-analysis indicate that the CRP level might be used as a biomarker to determine the severity of DR. © 2015 Song et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Yu Y.,Tianjin Medical University | Yang T.,Tianjin Medical University | Wang L.,Tianjin Medical University | Sun R.,Tianjin Key Laboratory of Ophthalmology and Visual Science | And 5 more authors.
Shock | Year: 2016

Gastrointestinal barrier dysfunction is associated with the severity and prognosis of sepsis. Hydrogen gas ( H2 ) can ameliorate multiple organ damage in septic animals. Ras homolog gene family member A (RhoA) and mammalian diaphanous-related formin 1 (mDia1) are important to regulate tight junction (TJ) and adherens junction (AJ), both of which determine the integrity of the intestinal barrier. This study was aimed to investigate whether H2 could modulate lipopolysaccharide (LPS)-stimulated dysfunction of the intestinal barrier and whether RhoA-mDia1 signaling is involved. Caco-2 cells were exposed to different concentrations of LPS (1μg/mL-1 mg/mL). The permeability of the intestinal barrier was evaluated by transepithelial resistance (TER) and fluorescein-isothiocyanate-dextran flux. Expression and distribution of occludin and E-cadherin were analyzed by Western blot and immunofluorescence. RhoA activity was measured by G-Lisa assay, and mDia1 expression was assessed by Western blot. LPS (100μg/mL) decreased TER and increased fluoresceinisothiocyanate-dextran flux, which were alleviated by H2-rich medium. Also, H2 down-regulated LPS-induced oxidative stress. Moreover, H2 improved the down-regulated expression and redistribution of occludin and E-cadherin caused by LPS. Additionally, H2 alleviated LPS-caused RhoA activation, and the beneficial effects of H2 on barrier were counteracted by RhoA agonist CN03. Rho inhibitor C3 exoenzyme mitigated LPS-induced barrier breakdown. Furthermore, H2-rich medium increased mDia1 expression, and mDia1 knockdown abolished protections of H2 on barrier permeability. mDia1 knockdown eliminated H2-induced benefits for occludin and E-cadherin. These findings suggest that H2 improves LPS-induced hyperpermeability of the intestinal barrier and disruptions of TJ and AJ by moderating RhoA-mDia1 signaling. Copyright © 2015 by the Shock Society.

Hong Y.,Tianjin Medical University | Sun L.,General Hospital of Beijing Military Command | Sun R.,Tianjin Key Laboratory of Ophthalmology and Visual Science | Chen H.,Tianjin Medical University | And 3 more authors.
Experimental and Therapeutic Medicine | Year: 2016

Multiple organ dysfunction syndrome (MODS) is a leading cause of mortality in critically ill patients. Hyperoxia treatment may be beneficial to critically ill patients. However, the clinical use of hyperoxia is hindered as it may exacerbate organ injury by increasing reactive oxygen species (ROS). Hydrogen gas (H2) exerts a therapeutic antioxidative effect by selectively reducing ROS. Combination therapy of H2 and hyperoxia has previously been shown to significantly improve survival rate and organ damage extent in mice with polymicrobial sepsis. The aim of the present study was to investigate whether combination therapy with H2 and hyperoxia could improve survival rate and organ damage in a zymosan (ZY)-induced generalized inflammation model. The results showed that the inhalation of H2 (2%) or hyperoxia (98%) alone improved the 14-day survival rate of ZY-challenged mice from 20 to 70 or 60%, respectively. However, combination therapy with H2 and hyperoxia could increase the 14-day survival rate of ZY-challenged mice to 100%. Furthermore, ZY-challenged mice showed significant multiple organ damage characterized by increased serum levels of aspartate transaminase, alanine transaminase, blood urea nitrogen and creatinine, as well as lung, liver and kidney histopathological scores at 24 h after ZY injection. These symptoms where attenuated by H2 or hyperoxia alone; however, combination therapy with H2 and hyperoxia had a more marked beneficial effect against lung, liver and kidney damage in ZY-challenged mice. In addition, the beneficial effects of this combination therapy on ZY-induced organ damage were associated with decreased serum levels of the oxidative product 8-iso-prostaglandin F2α, increased activity of superoxide dismutase and reduced levels of the proinflammatory cytokines high-mobility group box 1 and tumor necrosis factor-α. In conclusion, combination therapy with H2 and hyperoxia provides enhanced therapeutic efficacy against multiple organ damage in a ZY-induced generalized inflammation model, suggesting the potential applicability of H2 and hyperoxia in the therapy of conditions associated with inflammation-related MODS. © 2016, Spandidos Publications. All rights reserved.

Ying M.,Tianjin Eye Hospital | Ying M.,Tianjin Key Laboratory of Ophthalmology and Visual Science | Han R.,Tianjin Eye Hospital | Han R.,Tianjin Eye Institute | And 8 more authors.
BMC Medical Genetics | Year: 2013

Background: Mutations in the KIF21A gene are detected in the patients with congenital fibrosis of the extraocular muscles. Mutations in the PAX6 gene are detected in the patients with congenital aniridia.Case presentation: Herein we report a boy with both congenital fibrosis of extraocular muscles and aniridia. Sequence analysis of his KIF21A and PAX6 genes reveals a 1-bp deletion (c.745delC) in the PAX6 gene and a missense mutation of c.2860C > T (p.Arg954Trp) in KIF21A.Conclusions: This study demonstrates that the occurrence of independent mutations in more than a single gene in a patient may lead to a complex phenotype. © 2013 Ying et al.; licensee BioMed Central Ltd.

Wei S.,Tianjin Medical University | Wei S.,Tianjin Key Laboratory of Ophthalmology and Visual Science | Song H.,Tianjin Medical University | Song H.,Tianjin Key Laboratory of Ophthalmology and Visual Science | And 3 more authors.
Cornea | Year: 2014

PURPOSE: The aim of this study was to investigate the distribution of higher-order aberrations (HOAs) of the anterior cornea of eyes with cataract in a senior Chinese population and their correlations with age. METHODS: Four hundred ten Chinese patients (male/female: 196/214; age: 67.9 ± 10.0 years) with cataract were categorized into 5 groups according to their age. The HOAs in the central 6-mm zone of the cornea were measured with an iTrace wavefront analyzer. The correlations between age and total HOA, coma aberration, trefoil aberration, and spherical aberration were assessed using Spearman analysis. The differences in total HOA, coma aberration, trefoil aberration, spherical aberration among various age groups were analyzed using the Kruskal-Wallis H test. RESULTS: The corneal HOA exhibited a nonnormal distribution. A weak correlation was found between age and total HOA, coma aberration (Z3±3), trefoil aberration (Z 3±1) (P < 0.001 for all). No correlation was found between age and spherical aberration (Z40). Differences in the root mean square of the total HOA, Z3 ±1, and Z3±3 were found among various age groups (P < 0.05). No difference was found in the root mean square of Z40, spherical diopter, corneal curvature, corneal astigmatism, corneal effective refractive power, corneal asphericity Q value and corneal central power across the groups. CONCLUSIONS: The distribution of anterior corneal HOAs of eyes with cataract in a senior Chinese population is of the nonnormal type. The total HOA, Z3±1, and Z3±3 are correlated to age, unlike Z4 of the anterior cornea. Copyright © 2014 by Lippincott Williams & Wilkins.

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