Tianjin Key Laboratory of Molecular Drug Research

Tianjin, China

Tianjin Key Laboratory of Molecular Drug Research

Tianjin, China
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Liu R.,Tianjin Key Laboratory of Molecular Drug Research | Xu Y.,Key Laboratory of Original Agro environment Quality of Ministry of Agriculture | Jiang H.,CAS Institute of Zoology | Qiao C.,CAS Institute of Zoology | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2014

The twin-arginine translocation (Tat) pathway exports folded proteins across the cytoplasmic membranes of bacteria and archaea. Two parallel Tat pathways (TatAdCd and TatAyCy systems) with distinct substrate specificities have previously been discovered in Bacillus subtilis. In this study, to secrete methyl parathion hydrolase (MPH) into the growth medium, the twin-arginine signal peptide of B. subtilis YwbN was used to target MPH to the Tat pathway of B. subtilis. Western blot analysis and MPH assays demonstrated that active MPH was secreted into the culture supernatant of wild-type cells. No MPH secretion occurred in a total-tat2 mutant, indicating that the observed export in wild-type cells was mediated exclusively by the Tat pathway. Export was fully blocked in a tatAyCy mutant. In contrast, the tatAdCd mutant was still capable of secreting MPH. These results indicated that the MPH secretion directed by the YwbN signal peptide was specifically mediated by the TatAyCy system. The N-terminal sequence of secreted MPH was determined as AAPQVR, demonstrating that the YwbN signal peptide had been processed correctly. This is the first report of functional secretion of a heterologous protein via the B. subtilis TatAyCy system. This study highlights the potential of the TatAyCy system to be used for secretion of other heterologous proteins in B. subtilis. © 2014 American Chemical Society.

Xu J.,Tianjin Key Laboratory of Molecular Drug Research | Jin D.-Q.,Nankai University | Liu C.,Tianjin Key Laboratory of Molecular Drug Research | Xie C.,Tianjin Key Laboratory of Molecular Drug Research | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

Blumea balsamifera belongs to the family Compositae, and its leaves have been used as a flavoring ingredient and a tea. A phytochemical investigation of the aerial parts of B. balsamifera led to the isolation of 10 new (1-10) and 1 known (11) sesquiterpenes. Their structures were elucidated on the basis of extensive one- and two-dimensional nuclear magnetic resonance (heteronuclear multiple-quantum coherence, heteronuclear multiple-bond correlation, 1H-1H correlation spectroscopy, and nuclear Overhauser effect spectrometry) spectroscopic data analyses, and the structure of compound 1 was confirmed by X-ray crystallography. The inhibitory activities on lipopolysaccharide-induced NO production in murine microglial BV-2 cells of these sesquiterpenes were evaluated, and all of the compounds showed inhibitory effects. © 2012 American Chemical Society.

Cai Y.,Tianjin Key Laboratory of Molecular Drug Research | Cai Y.,Nankai University | Zhan J.,Tianjin Key Laboratory of Molecular Drug Research | Shen H.,Nankai University | And 8 more authors.
Analytical Chemistry | Year: 2016

We report in this study on optimized ratiometric fluorescent probes by peptide self-assembly. The resulting self-assembled nanoprobes show extraordinary stability in aqueous solutions and extremely low background fluorescence in buffer solutions. Our optimized probes with much bigger ratiometric fluorescence ratios also show an enhanced cellular uptake, lower background noise, and much brighter fluorescence signal in the cell experiment. Our study provides a versatile and very useful strategy to design and produce fluorescent probes with better performance. © 2015 American Chemical Society.

Wang H.,Nankai University | Shi Y.,Nankai University | Wang L.,Tianjin Key Laboratory of Molecular Drug Research | Yang Z.,Nankai University
Chemical Society Reviews | Year: 2013

Protein-based hydrogels are promising materials for tissue engineering and drug delivery due to the unique properties of proteins such as perfect polydispersity, exact control over monomer sequence, ability to fine-tune molecular-level biochemical interactions, etc. This tutorial review summarizes recent progress on the preparation of protein-based hydrogels and their applications. Typically, we introduce two strategies of covalent and non-covalent ones for the preparation of hydrogels. Hydrogels prepared by the covalent strategy are stable and can respond to the conformational change of proteins. They can be applied for cells encapsulation, screening of drug molecules and heavy metals, etc. Hydrogels formed by non-covalent interactions are injectable physical hydrogels. The simple mixing preparation strategy and fast gelation kinetics guarantee the homogeneous encapsulation of cells and therapeutic agents within them. Therefore, they have been widely applied for the delivery of bioactive components, regenerative medicine, etc. The challenges that remained in this field are also summarized in this paper. We envision that rationally designed protein-based hydrogels will have broad applications in many areas including controlled delivery, tissue engineering, drug screening, etc. © 2013 The Royal Society of Chemistry.

Fu X.,Central South University | Fu X.,Nanchang University | Meng M.,Tianjin Key Laboratory of Molecular Drug Research | Zhang Y.,Tianjin Key Laboratory of Molecular Drug Research | And 3 more authors.
Analytica Chimica Acta | Year: 2012

To detect a biomarker for small cell lung carcinoma, neuron specific enolase (NSE), a sensitive and specific chemiluminescence enzyme immunoassay was developed. Fluorescein isothiocyanate (FITC) labeled NSE capture antibody connected with NSE and alkaline phosphatase (ALP) labeled NSE detection antibody in a sandwich-type detection manner. This immune complex was further reacted with anti-FITC coated magnetic beads. In a magnetic field, the complex was enriched, and the sensitivity was thus enhanced. The limit of detection (LOD) of this method was <0.2ngmL -1. The proposed immunoassay was highly selective, and not interfered by hook effect. The recovery was >83.0% and the coefficient of variation was <10.0%. Human sera from 120 patients were tested with the presented and traditional chemiluminescence enzyme immunoassay. An excellent linear relationship was obtained between two techniques. Overall, this immunoassay offers a promising alternative for NSE detection than traditional clinical examinations. © 2012 Elsevier B.V..

Cui X.-A.,Tianjin Key Laboratory of Molecular Drug Research | Cui X.-A.,Urologic | Wang L.,Tianjin Key Laboratory of Molecular Drug Research | Kong D.-L.,Tianjin Key Laboratory of Molecular Drug Research | Gu H.-Q.,Urologic
Chinese Journal of Biomedical Engineering | Year: 2011

Vascular endothelial growth factor (VEGF) is a type of peptide regulating the activity of vascular endothelial cells and plays an important role in tissue regeneration and organ remodeling. However, due to its short half-life and fast degradation in vivo, its clinical application has been limited. This article described several VEGF controlled release systems, including microspheres, nanoparticles, hydrogels, tissue engineering scaffolds, and gene vectors. They had their own advantages for the controlled release of VEGF and broad research and application prospects in the promotion of tissue regeneration and repair.

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