Tianjin Key Laboratory of Food Biotechnology

Tianjin, China

Tianjin Key Laboratory of Food Biotechnology

Tianjin, China
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Zhang Y.,Tianjin University of Commerce | Zhang Y.,Tianjin Key Laboratory of Food Biotechnology | Qiao L.,Tianjin University of Commerce | Song M.,Tianjin University of Commerce | And 5 more authors.
Pharmacognosy Magazine | Year: 2014

Background: Ziziphi Spinosae Semen (ZSS), the seed of Ziziphus jujuba var. spinosa (Bunge) Hu ex H. F. Chow., is a traditional herb for insomnia and anxiety in eastern Asia. However, few researches have been concerned with its effect on ameliorating memory and learning performance.Objective: To investigate the constituents of ZSS water soluble extract and its ameliorating learning and memory in mice.Materials and Methods: A new high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed and validated to determine the main constituents in the extract. The effect of ZSS water soluble extract on memory and learning performance was investigated in mice by Y-maze and passive avoidance test.Results: The extract could significantly decrease the number of errors (NOE), and increase the transfer latency time (TLT) and electrical stimuli time (EST). In addition, spinosin, jujuboside A (JuA) and jujuboside B (JuB) were simultaneously identified and quantified in the extract. Their contents in the extract were as followed: Spinosin (223.51mg/g), JuA (63.76mg/g) and JuB (26.29mg/g).Conclusion: The extract played a promising role in ameliorating memory in mice with alcohol induced memory retrieval disorders, and might help to improve learning capacity to some extent. Spinosin, JuA and JuB were the predominant constituents, which might be mainly responsible for the definite activity.


Lu D.,Tianjin University of Commerce | Pang G.,Tianjin University of Commerce | Pang G.,Tianjin Key Laboratory of Food Biotechnology | Xie J.,Tianjin University of Commerce | Xie J.,Tianjin Key Laboratory of Food Biotechnology
Biomedical Microdevices | Year: 2017

In the current study, a novel double-layer gold nanoparticles- electrochemical immunosensor electrode (DGN-EIE) immobilized with Salmonella plasmid virulence C (SpvC) antibody was developed. To increase the fixed quantity of antibodies and electrochemical signal, an electrochemical biosensing signal amplification system was utilized with gold nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). In addition, the SpvC monoclonal antibodies (derived from Balb/c mice) were prepared and screened with a high affinity to SpvC. To evaluate the quality of DGN-EIE, the amperometric I-t curve method was applied to determine Salmonella in PBS. The results showed that the response current had a good linear correlation with the bacterial quantity ranged from 1.0 × 101–5.0 × 104 cfu/mL. The lowest detection limit was found at 5 cfu/mL. Furthermore, the proposed immunosensor has been demonstrated with high sensitivity, good selectivity and reproducibility. Apparently, DGN-EIE may be a very useful tool for monitoring the bacteria. © 2017, Springer Science+Business Media New York.


Wei L.,Tianjin University of Commerce | Qiao L.,Tianjin University of Commerce | Pang G.,Tianjin University of Commerce | Pang G.,Tianjin Key Laboratory of Food Biotechnology | And 2 more authors.
Biosensors and Bioelectronics | Year: 2017

At present, developing an efficient assay method for truly reflecting the real feelings of gustatory tissues is of great importance. In this study, a novel biosensor was fabricated to investigate the kinetic characteristics of the receptors in taste bud tissues sensing bitter substances for the first time. Porcine taste bud tissues were used as the sensing elements, and the sandwich-type sensing membrane was fixed onto a glassy carbon electrode for assembling the biosensor. With the developed sensor, the response currents induced by sucrose octaacetate, denatonium benzoate, and quercetin stimulating corresponding receptors were determined. The results demonstrated that the interaction between the analyst with their receptors were fitting to hyperbola (R2=0.9776, 0.9980 and 0.9601), and the activation constants were 8.748×10−15 mol/L, 1.429×10–12 mol/L, 6.613×10–14 mol/L, respectively. The average number of receptors per cell was calculated as 1.75, 28.58, and 13.23, while the signal amplification factors were 1.08×104, 2.89×103 and 9.76×104. These suggest that the sensor can be used to quantitatively describe the interaction characteristics of cells or tissue receptors with their ligands, the role of cellular signaling cascade, the number of receptors, and the signal transmission pathways. © 2017 Elsevier B.V.


Liu J.-F.,Tianjin University of Commerce | Liu J.-F.,Tianjin Key Laboratory of Food Biotechnology | Liu H.,Chinese Academy of Agricultural Sciences | Tan B.,Academy of State Administration of Grain | And 2 more authors.
Journal of Molecular Catalysis B: Enzymatic | Year: 2012

A nanosized support for reversible immobilization of enzymes, magnetic poly(glycidyl methacrylate-ethylene glycol dimethacrylate-hydroxyethyl methacrylate) nanospheres grafted with polyethyleneimine, was prepared and characterized by scanning electron microscopy and vibrating sample magnetometry. The novel support displayed 3.2-fold higher adsorption capacity for Kluyveromyces fragilis β-galactosidase than commercial ion exchange resin DEAE-Sepharose, the maximum amount of the enzyme adsorbed on the support reached 86.7 mg/g with enzyme activity recovery of 92.5%. The adsorbed K. fragilis β-galactosidase showed high catalytic activity and operational stability for synthesis of galacto-oligosaccharide (GOS), 4.5 kg of GOS were produced per gram of adsorbed enzyme from lactose during 15 consecutive batch reactions by reuse of the same biocatalyst, and the immobilized biocatalyst retained about 84.6% of its initial activity after 15 cycles of batch operation. The support could be regenerated and reused, remaining 93.0% of its original adsorption capacity at the end of the twentieth adsorption-desorption cycle. © 2012 Elsevier B.V.


Xie J.,Tianjin University of Commerce | Xie J.,Tianjin Key Laboratory of Food Biotechnology | Zhang Y.,Tianjin University of Commerce | Zhang Y.,Tianjin Key Laboratory of Food Biotechnology | And 2 more authors.
Journal of Food Composition and Analysis | Year: 2011

A new and rapid method was developed for simultaneous identification and determination of 11 polyphenols in Herba lycopi, i.e. rosmarinic acid, protocatechuic aldehyde, protocatechuic acid, caffeic acid, ferulic acid, apigenin, luteolin, quercetin, isorhamnetin, chlorogenic acid and rutin, using high performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry (MS/MS). Quantitation was based on multiple reactions monitoring (MRM) using the precursor→production combination for the determination of 11 analytes. The analysis was performed on an Eclipse Plus C18 column (I.D. 4.6mm×100mm, 3.5μm particle size). An electrospray ionization (ESI)-tandem interface in the negative mode was employed prior to mass spectrometric detection. With the optimized conditions, the 11 biomarkers were detected properly within 3min. Limits of detection (LOD) ranged from 0.6 to 2.6ng/ml. The average recoveries ranged from 95.42 to 101.11% with RSDs≤2.23%. The applicability of this analytical approach was confirmed by the successful analysis of 3 samples. The established method was validated and found to be sensitive and reliable for the determination of 11 analytes in Herba lycopi. © 2011 Elsevier Inc.


Zhang Y.,Tianjin University of Commerce | Zhang Y.,Tianjin Key Laboratory of Food Biotechnology | Ma G.,Tianjin University of Commerce | Xie J.,Tianjin University of Commerce | Xie J.,Tianjin Key Laboratory of Food Biotechnology
Journal of Liquid Chromatography and Related Technologies | Year: 2015

Jujuboside A (JuA), a dammarane-type triterpene glycoside, is a main bioactive saponin in Zizyphi Spinosi Semen (a traditional Chinese herbal food). In this research, the distribution of JuA in Sprague-Dawley rats was investigated with a new and efficient HPLC-ESI-MS/MS method. The results showed that JuA distributed rapidly and widely in various rat tissues (including heart, liver, spleen, kidney, and lung) after intravenous administration. In addition, it was initially found that JuA could pass through the blood-brain barrier and reach various areas of the brain quickly. At 0.25 hr, the concentration of JuA in the hippocampus (204.10 ng·g-1) was significantly higher than in the cerebrum (144.27 ng·g-1), olfactory bulb (98.42 ng·g-1), and corpus striatum (76.04 ng·g-1) (p < 0.05), indicating that the hippocampus was the main accumulation area of JuA in the brain. © 2015 Taylor & Francis Group, LLC.


Zhang Y.,Tianjin University of Commerce | Zhang Y.,Tianjin Key Laboratory of Food Biotechnology | Zhang K.,Tianjin University of Commerce | Zhang K.,Tianjin Key Laboratory of Food Biotechnology | And 5 more authors.
Journal of Chromatographic Science | Year: 2014

Jujuboside B (JuB) is a main bioactive saponin constituent of Ziziphi Spinosae Semen, which is a traditional herb for the treatment of insomnia and anxiety. However, the detailed metabolic mechanism of JuB is poorly understood. In this study, a novel method of rapid resolution liquid chromatography-triple quadrupole mass spectrometry was developed and validated for the analysis of JuB. With the method, the degradation kinetics of JuB by rat intestinal flora in vitro was investigated. The analysis was performed with an Agilent Eclipse Plus C18 (2.1 mm × 50 mm, 1.8 mm) column and an aqueous mobile phase (containing 0.1% formic acid) modified by methanol. The analyte was measured by multiple reaction-monitoring (MRM) modes with m/z 1043.3 × m/z 749.2. This method was validated with perfect accuracy, precision and limit of quantitation. It showed that jujuboside B (JuB) degradation started slowly as incubation with rat feces. The rate constant was correlated greatly with the concentration of sample solutions. Furthermore, some metabolites were elucidated with their chromatographic behavior and typical fragment ions. The results might help better interpret the metabolic and pharmacological mechanism of JuB. © The Author [2013]. Published by Oxford University Press. All rights reserved.


Zhang Y.,Tianjin University of Commerce | Xie J.,Tianjin University of Commerce | Xie J.,Tianjin Key Laboratory of Food Biotechnology | Zhang Y.,Tianjin Key Laboratory of Food Biotechnology | And 2 more authors.
International Journal of Food Properties | Year: 2014

Jujuboside A (JuA) is a representative saponin with significant sedative and hypnotic effects. Up to now, there were many arguments on its metabolic mechanism. In this study, a high performance liquid chromatography-tandem mass spectrometry (MS/MS) method was developed for investigating the degradation characteristics of JuA incubated with rat feces. With the method, the degradation kinetics of JuA was studied. The results showed JuA decomposed rapidly. It could decompose nearly 100% in only 4-6 h. The degradation regularity was consistent with the first dynamic process. In addition, seven metabolites were determined and identified to be the serial hydrolysis products of JuA. The results revealed that gastrointestinal microbiota played an indispensable role in the metabolic process of JuA. JuA may be a supplier of sugars under the hydrolysis effect induced by the bacterial enzymes. At the same time, its aglycone was produced as a by-product, which could be easier to be absorbed into the body to perform its specific bioactivities. Copyright © Taylor & Francis Group, LLC.


Wang X.-X.,Tianjin University of Commerce | Wang X.-X.,Tianjin Key Laboratory of Food Biotechnology | Ma G.-I.,Tianjin University of Commerce | Ma G.-I.,Tianjin Key Laboratory of Food Biotechnology | And 4 more authors.
Journal of Ethnopharmacology | Year: 2015

Ethnopharmacological relevance Jujuboside A (JuA) is a main active ingredient of semen ziziphi spinosae, which can significantly reduce spontaneous activity in mammals, increase the speed of falling asleep, prolong the sleeping time as well as improve the sleeping efficiency. In this study, the mechanism and the pathway of the sedative and hypnotic effect of JuA were investigated. Materials and methods After being treated with JuA (in vitro), the rat's small intestine tissues cultures were used to stimulate the brain tissues. Then 27 cytokine levels were detected in the two kinds of tissue culture via liquid protein chip technology; In addition, the cultured hippocampal neurons of rat were treated with JuA, and γ-aminobutyric acid (GABA) receptor subunits (GABAAα1, GABAAα5, GABAAβ1 and GABABR1) mRNAs were evaluated by Real-time PCR. Results The levels of IL-1α, MIP-1α, IL-1β and IL-2 were reduced significantly after 3 h of treating the small intestine tissues with JuA (200 μl/ml), and the concentration change rates, in order, were -59.3%, -3.59%, -50.1% and -49.4%; these cytokines were transmitted to brain tissues 2 h later, which could lead to significant levels of reduction of IL-1α, IFN-γ, IP-10 and TNF-α; the concentration change rates were -62.4%, -25.7%, -55.2% and -38.5%, respectively. Further, the intercellular communication network diagram was mapped out, which could suggest the mechanism and the pathway of the sedative and hypnotic effect of JuA. The results also indicated that JuA (50 μl/ml) increased significantly GABAAα1 receptor mRNAs and reduced GABABR1, mRNAs in hippocampal neurons after 24 h of stimulation; however, all the mRNA transcription levels of GABAAα1,GABAAα5, GABAAβ1 and GABABR1 receptors increased significantly after 48 h. Conclusion JuA performed its specific sedative and hypnotic effect through not only adjusting GABA receptors subunit mRNAs expression, but also down-regulating the secretion of relevant inflammation cytokines on the intestinal mucosal system to affect the intercellular cytokine network between nerve cells in the brain. This mechanism is similar to that of melatonin. © 2014 Elsevier Ireland Ltd. All rights reserved.


Xie J.,Tianjin University of Commerce | Xie J.,Tianjin Key Laboratory of Food Biotechnology | Sang L.,Weifang Peoples Hospital | Zhang Y.,Tianjin University of Commerce | And 3 more authors.
Journal of Liquid Chromatography and Related Technologies | Year: 2015

A new HPLC-MS/MS method was developed and validated for simultaneous determination of stachydrine and leonurine (two main bioactive alkaloids) in Herba Leonuri and its succedaneum-Herba Lagopsis. Chromatographic separation was performed on an Agilent Eclipse Plus C18 (100 mm × 2.1 mm, 3.5 m) and methanol-0.1% formic acid solution (20:80) was used as a mobile phase at a flow rate of 0.2 mL/min. With positive electrospray ionization interface, the two alkaloids were detected and quantified by multiple reaction monitoring mode with the transitions of m/z 144.2.2→58.1 for stachydrine and m/z 312.2→181.1 for leonurine. The limits of detection of stachydrine and leonurine were 2.3 and 1.0 pg, respectively. The applicability of this analytical approach was confirmed by the successful analysis of the samples of the two herbs. The established method was validated to be sensitive and reliable for the determination of stachydrine and leonurine in Herba Leonuri. It was demonstrated that Herba Lagopsis also comprised stachydrine and leonurine, although their contents were all lower than Herba Leonuri samples gathered from the same areas. © 2015 Taylor & Francis Group, LLC.

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