Tianjin Key Laboratory of Food Biotechnology

Tianjin, China

Tianjin Key Laboratory of Food Biotechnology

Tianjin, China

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Zhao X.,Tianjin University of Commerce | Liu J.,Tianjin University of Commerce | Liu J.,Tianjin Key Laboratory of Food Biotechnology | Wen Z.,Tianjin University of Commerce | And 7 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2017

Zizyphi Spinosi Semen (ZSS) has a long history of sedative-hypnotic use in China. As a novel flavone C-glycoside, coumaroylspinosin is a main flavonoid only found in ZSS. Up to now, its pharmacokinetic information and tissue distribution in vivo are not available yet. With a simple, rapid and sensitive HPLC–MS/MS method, the pharmacokinetics and tissue distribution of coumaroylspinosin were investigated in Sprague-Dawley (SD) rats after its intravenous administration. Puerarin was used as the internal standard (IS). The samples were extracted by a simple protein precipitation method with methanol. The MS analysis was performed with multiple reaction monitoring (MRM), and the transitions were set at m/z 753.3 → 427.0 for coumaroylspinosin and m/z 415.3 → 295.3 for IS, respectively. The method was successfully applied for investigating the pharmacokinetics and tissue distribution of coumaroylspinosin in Sprague Dawley (SD) rats after tail vein injection with 4.0 mg/kg of the flavonoid. The calibration curves covered over the range of 0.02–10 μg/mL in plasma and various tissues samples with good linearity(r ≥ 0.9956). The lower limit of quantification (LLOQ) in all samples was less than 20 ng/mL. The intra- and inter-day precisions were below 15% and accuracy was from −3.78% to 4.68%. No significant matrix effect was observed, and the average extraction recovery was acceptable. Coumaroylspinosin could be cleared quickly from the rat plasma with the half-life (t1/2) of 1.86 ± 0.15 h. It was distribute widely in vivo, and the main tissue depots of coumaroylspinosin in rats were found to be intestine, muscle and lung. With the method, the pharmacokinetic parameters and tissue distribution of coumaroylspinosin in SD rats were investigated for the first time. The results demonstrated that coumaroylspinosin was distributed widely and rapidly in various rat tissues after intravenous administration. © 2017 Elsevier B.V.


Liu J.-F.,Tianjin University of Commerce | Liu J.-F.,Tianjin Key Laboratory of Food Biotechnology | Liu H.,Chinese Academy of Agricultural Sciences | Tan B.,Academy of State Administration of Grain | And 2 more authors.
Journal of Molecular Catalysis B: Enzymatic | Year: 2012

A nanosized support for reversible immobilization of enzymes, magnetic poly(glycidyl methacrylate-ethylene glycol dimethacrylate-hydroxyethyl methacrylate) nanospheres grafted with polyethyleneimine, was prepared and characterized by scanning electron microscopy and vibrating sample magnetometry. The novel support displayed 3.2-fold higher adsorption capacity for Kluyveromyces fragilis β-galactosidase than commercial ion exchange resin DEAE-Sepharose, the maximum amount of the enzyme adsorbed on the support reached 86.7 mg/g with enzyme activity recovery of 92.5%. The adsorbed K. fragilis β-galactosidase showed high catalytic activity and operational stability for synthesis of galacto-oligosaccharide (GOS), 4.5 kg of GOS were produced per gram of adsorbed enzyme from lactose during 15 consecutive batch reactions by reuse of the same biocatalyst, and the immobilized biocatalyst retained about 84.6% of its initial activity after 15 cycles of batch operation. The support could be regenerated and reused, remaining 93.0% of its original adsorption capacity at the end of the twentieth adsorption-desorption cycle. © 2012 Elsevier B.V.


Xie J.,Tianjin University of Commerce | Xie J.,Tianjin Key Laboratory of Food Biotechnology | Zhang Y.,Tianjin University of Commerce | Zhang Y.,Tianjin Key Laboratory of Food Biotechnology | And 2 more authors.
Journal of Food Composition and Analysis | Year: 2011

A new and rapid method was developed for simultaneous identification and determination of 11 polyphenols in Herba lycopi, i.e. rosmarinic acid, protocatechuic aldehyde, protocatechuic acid, caffeic acid, ferulic acid, apigenin, luteolin, quercetin, isorhamnetin, chlorogenic acid and rutin, using high performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry (MS/MS). Quantitation was based on multiple reactions monitoring (MRM) using the precursor→production combination for the determination of 11 analytes. The analysis was performed on an Eclipse Plus C18 column (I.D. 4.6mm×100mm, 3.5μm particle size). An electrospray ionization (ESI)-tandem interface in the negative mode was employed prior to mass spectrometric detection. With the optimized conditions, the 11 biomarkers were detected properly within 3min. Limits of detection (LOD) ranged from 0.6 to 2.6ng/ml. The average recoveries ranged from 95.42 to 101.11% with RSDs≤2.23%. The applicability of this analytical approach was confirmed by the successful analysis of 3 samples. The established method was validated and found to be sensitive and reliable for the determination of 11 analytes in Herba lycopi. © 2011 Elsevier Inc.


Zhang Y.,Tianjin University of Commerce | Zhang Y.,Tianjin Key Laboratory of Food Biotechnology | Ma G.,Tianjin University of Commerce | Xie J.,Tianjin University of Commerce | Xie J.,Tianjin Key Laboratory of Food Biotechnology
Journal of Liquid Chromatography and Related Technologies | Year: 2015

Jujuboside A (JuA), a dammarane-type triterpene glycoside, is a main bioactive saponin in Zizyphi Spinosi Semen (a traditional Chinese herbal food). In this research, the distribution of JuA in Sprague-Dawley rats was investigated with a new and efficient HPLC-ESI-MS/MS method. The results showed that JuA distributed rapidly and widely in various rat tissues (including heart, liver, spleen, kidney, and lung) after intravenous administration. In addition, it was initially found that JuA could pass through the blood-brain barrier and reach various areas of the brain quickly. At 0.25 hr, the concentration of JuA in the hippocampus (204.10 ng·g-1) was significantly higher than in the cerebrum (144.27 ng·g-1), olfactory bulb (98.42 ng·g-1), and corpus striatum (76.04 ng·g-1) (p < 0.05), indicating that the hippocampus was the main accumulation area of JuA in the brain. © 2015 Taylor & Francis Group, LLC.


Zhang Y.,Tianjin University of Commerce | Zhang Y.,Tianjin Key Laboratory of Food Biotechnology | Zhang K.,Tianjin University of Commerce | Zhang K.,Tianjin Key Laboratory of Food Biotechnology | And 5 more authors.
Journal of Chromatographic Science | Year: 2014

Jujuboside B (JuB) is a main bioactive saponin constituent of Ziziphi Spinosae Semen, which is a traditional herb for the treatment of insomnia and anxiety. However, the detailed metabolic mechanism of JuB is poorly understood. In this study, a novel method of rapid resolution liquid chromatography-triple quadrupole mass spectrometry was developed and validated for the analysis of JuB. With the method, the degradation kinetics of JuB by rat intestinal flora in vitro was investigated. The analysis was performed with an Agilent Eclipse Plus C18 (2.1 mm × 50 mm, 1.8 mm) column and an aqueous mobile phase (containing 0.1% formic acid) modified by methanol. The analyte was measured by multiple reaction-monitoring (MRM) modes with m/z 1043.3 × m/z 749.2. This method was validated with perfect accuracy, precision and limit of quantitation. It showed that jujuboside B (JuB) degradation started slowly as incubation with rat feces. The rate constant was correlated greatly with the concentration of sample solutions. Furthermore, some metabolites were elucidated with their chromatographic behavior and typical fragment ions. The results might help better interpret the metabolic and pharmacological mechanism of JuB. © The Author [2013]. Published by Oxford University Press. All rights reserved.


Zhang Y.,Tianjin University of Commerce | Xie J.,Tianjin University of Commerce | Xie J.,Tianjin Key Laboratory of Food Biotechnology | Zhang Y.,Tianjin Key Laboratory of Food Biotechnology | And 2 more authors.
International Journal of Food Properties | Year: 2014

Jujuboside A (JuA) is a representative saponin with significant sedative and hypnotic effects. Up to now, there were many arguments on its metabolic mechanism. In this study, a high performance liquid chromatography-tandem mass spectrometry (MS/MS) method was developed for investigating the degradation characteristics of JuA incubated with rat feces. With the method, the degradation kinetics of JuA was studied. The results showed JuA decomposed rapidly. It could decompose nearly 100% in only 4-6 h. The degradation regularity was consistent with the first dynamic process. In addition, seven metabolites were determined and identified to be the serial hydrolysis products of JuA. The results revealed that gastrointestinal microbiota played an indispensable role in the metabolic process of JuA. JuA may be a supplier of sugars under the hydrolysis effect induced by the bacterial enzymes. At the same time, its aglycone was produced as a by-product, which could be easier to be absorbed into the body to perform its specific bioactivities. Copyright © Taylor & Francis Group, LLC.


Wang X.-X.,Tianjin University of Commerce | Wang X.-X.,Tianjin Key Laboratory of Food Biotechnology | Ma G.-I.,Tianjin University of Commerce | Ma G.-I.,Tianjin Key Laboratory of Food Biotechnology | And 4 more authors.
Journal of Ethnopharmacology | Year: 2015

Ethnopharmacological relevance Jujuboside A (JuA) is a main active ingredient of semen ziziphi spinosae, which can significantly reduce spontaneous activity in mammals, increase the speed of falling asleep, prolong the sleeping time as well as improve the sleeping efficiency. In this study, the mechanism and the pathway of the sedative and hypnotic effect of JuA were investigated. Materials and methods After being treated with JuA (in vitro), the rat's small intestine tissues cultures were used to stimulate the brain tissues. Then 27 cytokine levels were detected in the two kinds of tissue culture via liquid protein chip technology; In addition, the cultured hippocampal neurons of rat were treated with JuA, and γ-aminobutyric acid (GABA) receptor subunits (GABAAα1, GABAAα5, GABAAβ1 and GABABR1) mRNAs were evaluated by Real-time PCR. Results The levels of IL-1α, MIP-1α, IL-1β and IL-2 were reduced significantly after 3 h of treating the small intestine tissues with JuA (200 μl/ml), and the concentration change rates, in order, were -59.3%, -3.59%, -50.1% and -49.4%; these cytokines were transmitted to brain tissues 2 h later, which could lead to significant levels of reduction of IL-1α, IFN-γ, IP-10 and TNF-α; the concentration change rates were -62.4%, -25.7%, -55.2% and -38.5%, respectively. Further, the intercellular communication network diagram was mapped out, which could suggest the mechanism and the pathway of the sedative and hypnotic effect of JuA. The results also indicated that JuA (50 μl/ml) increased significantly GABAAα1 receptor mRNAs and reduced GABABR1, mRNAs in hippocampal neurons after 24 h of stimulation; however, all the mRNA transcription levels of GABAAα1,GABAAα5, GABAAβ1 and GABABR1 receptors increased significantly after 48 h. Conclusion JuA performed its specific sedative and hypnotic effect through not only adjusting GABA receptors subunit mRNAs expression, but also down-regulating the secretion of relevant inflammation cytokines on the intestinal mucosal system to affect the intercellular cytokine network between nerve cells in the brain. This mechanism is similar to that of melatonin. © 2014 Elsevier Ireland Ltd. All rights reserved.


Qiao L.,Tianjin University of Commerce | Jiao L.,Tianjin University of Commerce | Pang G.,Tianjin University of Commerce | Pang G.,Tianjin Key Laboratory of Food Biotechnology | And 2 more authors.
Biosensors and Bioelectronics | Year: 2015

A novel taste biosensor based on ligand-receptor interaction was developed through fixing taste-bud tissues of SD rats to a glassy carbon electrode. Using the sodium alginate-starch gel as a fixing agent, taste-bud tissues of SD rats were fixed between two nuclear microporous membranes to make a sandwich-type sensing membrane. With the taste biosensor, the response current induced by capsaicin and gingerol stimulating the corresponding receptors was measured. The results showed that the lowest limit of detection of this biosensor to capsaicin was 1×10-13mol/L and the change rate of response current was the highest at the concentration of 9×10-13mol/L, indicating that the capsaicin receptor was saturated at this point. The lowest limit of detection of this biosensor to gingerol was 1×10-12mol/L, and the gingerol receptor was saturated when the concentration of gingerol was 3×10-11mol/L. It was demonstrated that the interaction curves of capsaicin and gingerol with their respective receptors exhibited high correlation (R2: 0.9841 and 0.9904). The binding constant and dissociation constant of gingerol with its receptor were 1.564×10-11 and 1.815×10-11 respectively, which were all higher than those of capsaicin with its receptor (1.249×10-12 and 2.078×10-12). This study, for the first time, made it possible to quantitatively determine the interaction of the taste receptor and pungent substances with a new biosensor, thus providing a simple approach for monitoring pungent substances and investigating the mechanism of ligand-receptor interaction. © 2015 Elsevier B.V..


Xie J.,Tianjin University of Commerce | Xie J.,Tianjin Key Laboratory of Food Biotechnology | Sang L.,Weifang Peoples Hospital | Zhang Y.,Tianjin University of Commerce | And 3 more authors.
Journal of Liquid Chromatography and Related Technologies | Year: 2015

A new HPLC-MS/MS method was developed and validated for simultaneous determination of stachydrine and leonurine (two main bioactive alkaloids) in Herba Leonuri and its succedaneum-Herba Lagopsis. Chromatographic separation was performed on an Agilent Eclipse Plus C18 (100 mm × 2.1 mm, 3.5 m) and methanol-0.1% formic acid solution (20:80) was used as a mobile phase at a flow rate of 0.2 mL/min. With positive electrospray ionization interface, the two alkaloids were detected and quantified by multiple reaction monitoring mode with the transitions of m/z 144.2.2→58.1 for stachydrine and m/z 312.2→181.1 for leonurine. The limits of detection of stachydrine and leonurine were 2.3 and 1.0 pg, respectively. The applicability of this analytical approach was confirmed by the successful analysis of the samples of the two herbs. The established method was validated to be sensitive and reliable for the determination of stachydrine and leonurine in Herba Leonuri. It was demonstrated that Herba Lagopsis also comprised stachydrine and leonurine, although their contents were all lower than Herba Leonuri samples gathered from the same areas. © 2015 Taylor & Francis Group, LLC.


Zhang Y.,Tianjin University of Commerce | Zhang Y.,Tianjin Key Laboratory of Food Biotechnology | Xie J.,Tianjin University of Commerce | Xie J.,Tianjin Key Laboratory of Food Biotechnology | And 2 more authors.
Analytical Letters | Year: 2010

A new and rapid method was developed for simultaneous determination of mangiferin, neomangiferin, timosaponin A-III, and C in Rhizoma Anemarrhenae using rapid resolution liquid chromatography coupled with triple quadrupole mass spectrometry. The analysis was performed on an Eclipse Plus C18 column (I.D. 4.6×100mm, 3.5μm). Electrospray ionization-tandem interface in the negative mode was employed prior to mass spectrometric detection. Quantitation was based on multiple reaction monitoring (MRM) for determination. Limits of detection (LODs) ranged from 0.7 to 3 pg. The average recoveries ranged from 98.16 to 100.7% with RSDs ≤2.03%. The established method was validated, sensitive,and reliable. © Taylor & Francis Group, LLC.

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