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Zhang F.,Tianjin Huanhu Hospital | Zhang F.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | Zhao X.,Tianjin Medical University | Xu B.,Tianjin Medical University | And 6 more authors.
Analytical and Bioanalytical Chemistry | Year: 2016

Cell membrane chromatography (CMC) is a powerful tool to study membrane protein interactions and to screen active compounds extracted from natural products. Unfortunately, a large amount of cells are typically required for column preparation in order to carry out analyses in an efficient manner. Micro-CMC (mCMC) has recently been developed by using a silica capillary as a membrane carrier. However, a reduced retention of analytes is generally associated with mCMC mostly due to a low ligand (cellular membrane) capacity. To solve this common problem, in this work a silica-based porous layer open tubular (PLOT) capillary was fabricated and, to the best of our knowledge, for the first time applied to mCMC. The mCMC column was prepared by physical adsorption of rabbit red blood cell (rRBC) membranes onto the inner surface of the PLOT capillary. The effects of the PLOT capillaries fabricated by different feed compositions, on the immobilization amount of cellular membranes (represented by the fluorescence intensity of the capillary immobilized with fluorescein isothiocyanate isomer-labeled cellular membranes) and on the dynamic binding capacity (DBC) of verapamil (VP, a widely used calcium antagonist which specific interacts with L-type calcium channel proteins located on cellular membrane of rRBC) have been systematically investigated. The fluorescence intensity of the mCMC column when combined with the PLOT capillary was found to be more than five times higher than the intensity using a bare capillary. This intriguing result indicates that the PLOT capillary exhibits a higher cellular membrane capacity. The DBC of VP in the PLOT column was found to be more than nine times higher than that in the bare capillary. An rRBC/CMC column was also prepared for comparative studies. As a result, mCMC provides similar chromatographic retention factors and stability with common CMC; however, the cellular membrane consumption for mCMC was found to be more than 460 times lower than that for CMC. [Figure not available: see fulltext.] © 2016 Springer-Verlag Berlin Heidelberg Source


Lou Q.,Tianjin Huanhu Hospital | Lou Q.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | Liu S.,Tianjin Huanhu Hospital | Liu S.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | And 4 more authors.
Journal of Clinical Nursing | Year: 2015

Aims and objectives: The primary aim of this study was to examine the correlations between patient and caregiver characteristics with caregiver burden, anxiety and depression in Alzheimer's Disease. Secondary aim was to determine which behavioural and psychological symptoms had the greatest impact on caregiver burden, anxiety and depression in Alzheimer's Disease. Background: Caregivers of individuals with Alzheimer's Disease experience high levels of burden, both psychologically and physically. Previous studies have examined caregiver burden, anxiety and depression separately. However, no paper has examined these three psychological conditions simultaneously. Design: A cross-sectional design. Methods: A total of 310 patients with probable Alzheimer's Disease and their primary caregivers were assessed. Cognitive impairment and neuropsychiatric symptoms were assessed with the Mini Mental State Examination, Montreal Cognitive Assessment, Clock Drawing Test and Neuropsychiatric Inventory, respectively. Caregiver burden, anxiety and depression were assessed with the ZBI, Generalised Anxiety Disorder Scale-7 and Patient Health Questionnaire-9, respectively. Results: All 12 neuropsychiatric symptoms in the Neuropsychiatric Inventory were significantly correlated with caregiver burden, anxiety and depression, with the top three neuropsychiatric predictors being depression, apathy and anxiety. Furthermore, higher levels of caregiver anxiety were associated with a longer duration of being a caregiver. Within caregivers, higher levels of depression were independently associated with higher numbers of additional caregivers, lower educational background and being the spouse of the patient. Higher levels of burden were associated with a longer duration of being a caregiver and being the spouse of the patient. Caregiver burden, anxiety or depression were not significantly correlated with hours/day of caring for the patient. Conclusions: Caregiver burden, anxiety and depression were significantly correlated with different neuropsychiatric symptoms in the Neuropsychiatric Inventory. Relevance to clinical practice: Practitioners are able to identify caregivers at risk for burden, anxiety and depression. Understanding which Neuropsychiatric Inventory symptom is more closely associated with distress in caregivers will help practitioners to be more specific and effective in detecting caregiver distress. © 2015 John Wiley & Sons Ltd. Source


Yan H.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | Li B.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | Wang H.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | Kang J.-M.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases
Chinese Journal of Contemporary Neurology and Neurosurgery | Year: 2014

Due to its special anatomical structures and immune pathophysiological mechanisms, brain damage repair is greatly different from damage repair of other systems. Secondary brain injury and inflammation are closely related. As a "double-edged sword", inflammation scavenges hazardous substances on the early stage of injury, but has side effects on normal brain tissue. The use of immunosuppressive therapy or hypothermia can inhibit immune injury, but the presence of reduced immunity may result in infection and tumorigenesis in the long term. Only reducing the autoimmune attack against brain tissue without affecting other immune capacity of the body will be optimized solution, and this paper will make a review on the research of immune tolerance in the treatment of brain injury with optimized program. Source


Duo L.J.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | Rong J.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | Bin W.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | Hua M.C.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | And 2 more authors.
Oncology Letters | Year: 2015

Thyroid cancer is the most common endocrine malignancy, with an increasing prevalence worldwide. Poorly‑differentiated thyroid cancer (PDTC) is relatively rare and its prognosis is poor. To date, no ideal treatment strategy is available for patients with advanced recurrent PDTC, particularly for patients in crisis. However, partial success in treating thyroid cancer has been achieved with targeted therapy, and advances made in understanding the molecular biology of the tumor. The current study describes the case of a patient diagnosed with PDTC following presentation with hoarseness, orthopnea, and a large right neck mass. A transient partial response to sunitinib malate treatment was achieved for >3 months. In addition, the current study reviewed the relevant literature and discussed the therapeutic value of sunitinib as a more favorable treatment strategy for patients with advanced recurrent PDTC compared with the currently available treatments. Successful treatment with sunitinib, as well as molecular analysis of the tumor, occurred in the present case. Sunitinib was determined to have potential in treating thyroid tumors, however, larger prospective studies are required to validate the findings of the current case study prior to the application of this agent in clinical practice. © 2015, Spandidos Publications. All rights reserved. Source


Jiang R.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | Ma C.-H.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | Zhu Z.-L.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | Li J.-D.,Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases | And 3 more authors.
Chinese Journal of Contemporary Neurology and Neurosurgery | Year: 2014

Objective: To observe a new technology for the detection and enumeration of cerebrospinal fluid (CSF) circulating tumor cells (CTCs) in the diagnosis of non - small cell lung cancer (NSCLC) with meningeal metastasis (MM).Methods: Five cases of NSCLC with MM that were diagnosed by CSF cytology were selected, and 20 ml CSF samples were obtained by lumbar puncture for every patient. The tumor marker immunostaining-fluorescence in situ hybridization (TM-iFISH) technology was adapted to detect enrichment and enumeration of circulating tumor cells in 7.50 ml CSF samples; CSF cytology was checked in 10 ml CSF samples; CSF tumor markers were detected in 2.50 ml CSF samples. All of 5 cases were examined by MRI enhancement scan.Results: TM -iFISH detection found circulating tumor cells numbers ranging 18-1823/7.50 ml. Only 2 cases of patients with CSF cytology examination showed the tumor cells. The results of CSF tumor markers in all samples were higher than normal serum tumor markers detection results. The enhanced MRI scan of 5 cases revealed typical signs of MM.Conclusions: The TM - iFISH test showed certain advantages in the detection of malignant tumor cells in CSF. This technology may be a new method of detection and enumeration of tumor cells in CSF, but more studies are needed to prove its sensitivity and specificity. Source

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