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Zhi L.,Peking Union Medical College | Zhi L.,Tianjin Medical University | Zhi L.,Tianjin Key Laboratory of Cellular and Molecular Immunology | Zhi L.,Key Laboratory of Educational Ministry of China | And 12 more authors.
Oligonucleotides | Year: 2011

G-rich oligonucleotides (GROs) can inhibit cell proliferation by inducing cell cycle arrest at S phase in tumor cell lines. GROs bind specific cellular proteins, such as nucleolin, a crucial protein interacting with P53; however, little is known about the relationship between GROs and P53. In this study, we have shown that GROs inhibited the proliferation of U937 cells (a human monocytic leukemia cell line without P53 expression) by inducing S-phase arrest. We also showed that GRO colocalized with nucleolin in U937 cells. GRO treatment induced alteration of a series of cell cycle regulatory proteins in U937 cells. Increased Cdk2 expression might promote the cells to enter S phase and subsequent decrease of Cdk2 might induce cell cycle arrest in S phase. Transfection of U937 cells with a wild-type p53 gene caused the formation of nucleolin-P53 complex, which alleviated the effect of GRO on leukemia cells. This alleviated effect is probably due to the decreased uptake of GRO. © 2011, Mary Ann Liebert, Inc. 2011.

Liu X.Y.,Ministry of Health Key Laboratory of Hormone and Development | Liu X.Y.,Tianjin Medical University | Ge L.,Tianjin Medical University | Ge L.,Tianjin Key Laboratory of Cellular and Molecular Immunology | And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014

Purpose: In the present study, we constructed an efficient expression system for visfatin and identified the peptide sequences which binding to visfatin. Methods and results: The fusion protein was identified by SDS-PAGE and Western blot. The yield of visfatin was 300 mg/L culture medium with optimal conditions. The recombinant visfatin binds with insulin receptor with a dose-dependent effect. All of the 24 sequence identified by ELISA were able to bind to visfatin specifically. Among the 24 DNA sequences, there were 8 clones of AAKTPTE, 4 clones of ATTVPAS and 4 clones of MSLQQEH. Conclusion: The sequence of AA(X)TPT(X) was the most frequently existed sequence in all of sequences analyzed, which suggests that AA(X)TPT(X) is likely to be the essential motif in peptides which visfatin could bind with. © 2014, Int J Clin Exp Med.All right reserved.

Zhang Y.,Tianjin Medical University | Gao X.,Tianjin Medical University | Zhi L.,Tianjin Medical University | Liu X.,Tianjin Medical University | And 5 more authors.
Journal of Inorganic Biochemistry | Year: 2014

Sliver nanoparticles (NPs) possess wonderful antibacterial activity while ZnO is a promising photocatalyst. Although it has been reported that the photocatalytic activity of Ag/ZnO heterostructure nanoparticles (HNPs) was better than that of pure ZnO NPs, the existing comparisons on their antibacterial activity were all taken between Ag-doped ZnO HNPs and pure Ag NPs (or pure ZnO NPs), and were still controversial. Ag islands on ZnO, another type of Ag/ZnO HNPs, were synthesized by a two-step preparation and characterized in this work. The antibacterial activity of as-synthesized Ag/ZnO HNPs, especially with 5.0 wt.% of Ag, against Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) was enhanced greatly in contrast with that of the simple mixture of Ag and ZnO NPs. The enhancement was found to be mainly due to the obvious increase in the reactive oxidative species (especially superoxide) and the increased damage to plasmid DNA induced by Ag/ZnO HNPs. © 2013 Elsevier Inc.

Wang F.,Tianjin Medical University | Chang G.-M.,Tianjin Medical University | Geng X.,Tianjin Medical University | Geng X.,Tianjin Key Laboratory of Cellular and Molecular Immunology | Geng X.,Key Laboratory of Educational Ministry of China
European Review for Medical and Pharmacological Sciences | Year: 2014

OBJECTIVES: Alternative splicing of human telomerase reverse transcriptase (hTERT) has an important effect on regulating telomerase activity. Exonic splicing enhancers (ESEs) are a family of conserved splicing factors that participate in multiple steps of the splicing pathway. Our aim is to analyze the ESEs for predicting the potential regulatory elements of hTERT mRNA splicing. MATERIALS AND METHODS: Enter the FASTA format of hTERT total sequences or individual exon as the input data in the main interface of ESEfinder3.0 and ESEfinder2.0 program. Analyze the data of output results and compare the differences between ESEfinder3.0 and ESEfinder2.0 program. RESULTS: Five ESEs were predicted in exon 5 to exon 9 of hTERT. They were at position 108 located in hTERT exon 5, at position 92 located in exon 6, at position 22 located in exon 7, at position 73 located in exon 8 and at position 5 located in exon 9. There were no differences between ESEfinder 3.0 and ESEfinder 2.0 in our case. CONCLUSIONS: The identification of these potential ESEs of hTERT might be helpful for the design of antisense oligonucleotides, which could modulate hTERT alternative splicing and inhibit telomerase activity.

Wang F.,Tianjin Medical University | Chang G.,Tianjin Medical University | Geng X.,Tianjin Medical University | Geng X.,Tianjin Key Laboratory of Cellular and Molecular Immunology | Geng X.,Key Laboratory of Educational Ministry of China
PLoS ONE | Year: 2014

Vascular dementia (VaD) is a mental disorder caused by brain damage due to cerebrovascular disease, and incidence of VaD is rising. To date, there is no known effective cure for VaD, so effort in developing an effective treatment for VaD is of great importance. The differentiation plasticity of BMSCs, in conjunction with its weak immunogenicity, makes manipulated BMSCs an attractive strategy for disease treatment. However, BMSCs often display disabled differentiation, premature aging, and unstable proliferation, reducing their neuroprotective function. These problems may be caused by the lack of telomerase activity in BMSCs. Our results show that NGF-TERT co-transfected BMSCs have a better therapeutic effect than BMSCs lacking NGF and TERT expression, demonstrated by significant improvements in learning and memory in VaD rats. The underlying mechanism might be increased expression of NGF, TrkA and SYN in the hippocampal CA1 area, which has potential implication in advancing therapeutics for VaD. © 2014 Wang et al.

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