Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury

Lincheng, China

Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury

Lincheng, China
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Zhang D.,Tianjin First Center Hospital | Liu B.,People's Care | Cao B.,Tianjin University | Cao B.,Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury | And 6 more authors.
International Immunopharmacology | Year: 2017

Pulmonary fibrosis, a progressive and lethal lung disease, is a major therapeutic challenge for which new therapeutic strategies are warranted. Schisandrin B (Sch B) and Glycyrrhizic acid (GA) are the principal active ingredients of Schisandra chinensis and Glycyrrhiza glabra respectively, which have been reported to protect against lung injures. The present study was aimed at exploring the combinatorial therapeutic effects on bleomycin-induced pulmonary fibrosis. Lung fibrotic injuries were induced in mice by a single intratracheal instillation of 5 mg/kg bleomycin (BLM). Then, these mice were administered with Sch B (100 mg/kg) or/and GA (75 mg/kg) for 28 days. BLM-triggered structure distortion, collagen overproduction, excessive inflammatory infiltration, pro-inflammatory cytokine release, and oxidative stress damages in lung tissues were attenuated to a higher degree by combinatorial treatment than by treatment of the individual agents. The expression of TGF-β1 and the phosphorylation of its downstream target, Smad2 were enhanced by BLM, but weakened by Sch B or/and GA. Furthermore, the significant overexpression of NADPH oxidase 4 (NOX4) was observed in BLM-induced pulmonary fibrosis, which was inhibited by Sch B or/and GA. Our study reveals that the synergistic protection by Sch B and GA against BLM-induced pulmonary fibrosis is correlated to its anti-inflammatory, anti-oxidative and anti-fibrotic properties, involving inhibition of TGF-β1/Smad2 signaling pathways and overexpression of NOX4. © 2017 Elsevier B.V.

Ji W.-J.,People's Care | Ji W.-J.,Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury | Ma Y.-Q.,People's Care | Ma Y.-Q.,Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury | And 14 more authors.
Journal of Immunological Methods | Year: 2014

The mononuclear phagocyte system, including circulating monocytes and tissue resident macrophages, plays an important role in acute lung injury and fibrosis. The detailed dynamic changes of mononuclear phagocytes in the circulating, lung alveolar and interstitial compartments in bleomycin-induced pulmonary injury model have not been fully characterized. The present study was designed to address this issue and analyzed their relationships with pulmonary pathological evolution after bleomycin challenge. A total of 100 male C57BL/6 mice were randomly divided to receive bleomycin (2.5mg/kg, n=50) or normal saline (n=50) via oropharyngeal approach, and were sacrificed on days 1, 3, 7, 14 and 21. Circulating monocyte subsets, polarization state of bronchoalveolar lavage fluid (BALF)-derived alveolar macrophages (AMφ) and lung interstitial macrophages (IMφ, derived from enzymatically digested lung tissue) were analyzed by flow cytometry. There was a rapid expansion of circulating Ly6Chi monocytes which peaked on day 3, and its magnitude was positively associated with pulmonary inflammatory response. Moreover, an expansion of M2-like AMφ (F4/80+CD11c+CD206+) peaked on day 14, and was positively correlated with the magnitude of lung fibrosis. The polarization state of IMφ remained relatively stable in the early- and mid-stage after bleomycin challenge, expect for an increase of M2-like (F4/80+CD11c-CD206+) IMφ on day 21. These results support the notion that there is a Ly6Chi-monocyte-directed pulmonary AMφ alternative activation. Our result provides a dynamic view of mononuclear phagocyte change in three compartments after bleomycin challenge, which is relevant for designing new treatment strategies targeting mononuclear phagocytes in this model. © 2013 Elsevier B.V.

Cao B.,Chinese People's Armed Police forces Academy | Chen H.,Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury | Gao Y.,Chinese People's Armed Police forces Academy | Niu C.,Chinese People's Armed Police forces Academy | And 2 more authors.
International Journal of Molecular Medicine | Year: 2015

The need to overcome cancer multidrug resistance (MDR) has fueled considerable interest in the development of novel synthetic antitumor agents with cytotoxicity against cancer cell lines with MDR. In this study, we aimed to investigate CIP-36, a novel podophyllotoxin derivative, for its inhibitory effects on human cancer cells from multiple sources, particularly cells with MDR in vitro. The human leukemia cell line, K562, and the adriamycin-resistant subline, K562/A02, were exposed to CIP-36 or anticancer agents, and various morphological and biochemical properties were assessed by Hoechst 33342 staining under a fluorescence microscope. Subsequently, cytotoxicity, cell growth curves and the cell cycle were analyzed. Finally, the effects of CIP-36 on topoisomerase IIα (Topo IIα) activity were determined. Treatment with CIP-36 significantly inhibited the growth of the K562 and MDR K562/A02 cells. Our data demonstrated that CIP-36 induced apoptosis, inhibited cell cycle progression and inhibited Topo IIα activity. These findings suggest that CIP-36 has the potential to overcome the multidrug resistance of K562/A02 cells by mediating Topo IIα activity.

Liu B.,People's Care | Cao B.,Tianjin University | Cao B.,Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury | Zhang D.,Tianjin First Center Hospital | And 5 more authors.
Toxicology and Applied Pharmacology | Year: 2016

The present study was aimed at exploring the protective effects of Salvianolic acid B (SalB) against paraquat (PQ)-induced lung injury in mice. Lung fibrotic injuries were induced in mice by a single intragastrical administration of 300 mg/kg PQ, then the mice were administrated with 200 mg/kg, 400 mg/kg SalB, 100 mg/kg vitamin C (Vit C) and dexamethasone (DXM) for 14 days. PQ-triggered structure distortion, collagen overproduction, excessive inflammatory infiltration, pro-inflammatory cytokine release, and oxidative stress damages in lung tissues and mortality of mice were attenuated by SalB in a dose-dependent manner. Furthermore, SalB was noted to enhance the expression and nuclear translocation of nuclear factor erythroid 2–related factor 2 (Nrf2) and reduce expression of the reactive oxygen species-generating enzyme Nox4 [NADPH (reduced form of nicotinamide adenine dinucleotide phosphate) oxidase-4]. SalB also inhibited the increasing expression of transforming growth factor (TGF)-β1 and the phosphorylation of its downstream target Smad3 which were enhanced by PQ. These results suggest that SalB may exert protective effects against PQ-induced lung injury and pulmonary fibrosis. Its mechanisms involve the mediation of Nrf2/Nox4 redox balance and TGF-β1/Smad3 signaling. © 2016

Zhou X.,Tianjin University | Zhou X.,Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury | Luo Y.-C.,Tianjin University | Luo Y.-C.,Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury | And 17 more authors.
PLoS ONE | Year: 2013

Background:Emerging evidence shows that anti-inflammatory strategies targeting inflammatory monocyte subset could reduce excessive inflammation and improve cardiovascular outcomes. Functional expression of voltage-gated sodium channels (VGSCs) have been demonstrated in monocytes and macrophages. We hypothesized that mononuclear phagocyte VGSCs are a target for monocyte/macrophage phenotypic switch, and liposome mediated inhibition of mononuclear phagocyte VGSC may attenuate myocardial ischemia/reperfusion (I/R) injury and improve post-infarction left ventricular remodeling.Methodology/Principal Findings:Thin film dispersion method was used to prepare phenytoin (PHT, a non-selective VGSC inhibitor) entrapped liposomes. Pharmacokinetic study revealed that the distribution and elimination half-life of PHT entrapped liposomes were shorter than those of free PHT, indicating a rapid uptake by mononuclear phagocytes after intravenous injection. In rat peritoneal macrophages, several VGSC α subunits (NaV1.1, NaV1.3, NaV1.4, NaV1.5, NaV1.6, NaV1.7, NaVX, Scn1b, Scn3b and Scn4b) and β subunits were expressed at mRNA level, and PHT could suppress lipopolysaccharide induced M1 polarization (decreased TNF-α and CCL5 expression) and facilitate interleukin-4 induced M2 polarization (increased Arg1 and TGF-β1 expression). In vivo study using rat model of myocardial I/R injury, demonstrated that PHT entrapped liposome could partially suppress I/R injury induced CD43+ inflammatory monocyte expansion, along with decreased infarct size and left ventricular fibrosis. Transthoracic echocardiography and invasive hemodynamic analysis revealed that PHT entrapped liposome treatment could attenuate left ventricular structural and functional remodeling, as shown by increased ejection fraction, reduced end-systolic and end-diastolic volume, as well as an amelioration of left ventricular systolic (+dP/dtmax) and diastolic (-dP/dtmin) functions.Conclusions/Significance:Our work for the first time demonstrates the therapeutic potential of VGSC antagonism via liposome mediated monocyte/macrophage targeting in acute phase after myocardial I/R injury. These results suggest that VGSCs in mononuclear phagocyte system might be a novel target for immunomodulation and treatment of myocardial I/R injury. © 2013 Zhou et al.

Li H.,Tianjin University | Li H.,Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard | Fan R.,Tianjin University | Sun M.,Tianjin University | And 4 more authors.
Biochemical and Biophysical Research Communications | Year: 2013

Nspc1 is an identified transcription repressor. However, transiently up-regulated or down-regulated Nspc1 in P19 embryonal carcinoma cells affects expression levels of Oct4, Sox2 and Nanog in a positive correlation. Luciferase activity assays verified that Nspc1 regulates the Oct4 promoter in a dose dependent manner. ChIP assay shows that Nspc1 activates Oct4 by directly binding to the (-1021 to -784) region of Oct4 promoter. Dominant negative analysis indicated the activation is dependent on the retinoid acid response element (RARE). We demonstrated Nspc1 has a positive role in maintaining the pluripotency of P19 cells by directly regulating Oct4. © 2013 Elsevier Inc. All rights reserved.

Zhang Y.-Y.,Tianjin Medical University | Zhang Y.-Y.,Tianjin University | Zhou X.,Tianjin University | Zhou X.,Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury | And 20 more authors.
Journal of Thrombosis and Thrombolysis | Year: 2014

To investigate the relationship between circulating microRNA 223 (miR-223) levels and clopidogrel responsiveness in patients with coronary heart disease. A total of 62 consecutive patients with troponin-negative non-ST elevation acute coronary syndrome (NSTE-ACS) scheduled for elective percutaneous coronary intervention were enrolled. The plasma circulating miR-223 levels were quantified by real-time PCR, and platelet reactivity was determined by platelet reactivity index (PRI), measured by vasodilator-stimulated phosphoprotein (VASP) phosphorylation flow cytometry after 300 mg (for at least 24 h) or 75 mg clopidogel (for at least 5 days) plus aspirin treatment. All subjects were dichotomized according to PRI median (normal-responders: PRI ≤ 56.3 %, n = 31 and low-responders: PRI > 56.3 %, n = 31). Compared with normal-responders, circulating miR-223 level was significantly decreased in low-responders (P = 0.007). In addition, miR-223 level was statistically correlated with PRI (Spearman r = -0.379, P = 0.002). Stepwise binary logistic regression analysis revealed that among factors that potentially influence platelet reactivity (CYP2C19*2/*3 loss-of-function genotypes, use of calcium channel blockers/proton-pump inhibitors, age, diabetes and smoking), decreased circulating miR-223 level was the only independent predictor for the presence of PRI-determined lower responders (OR 0.111, 95 % CI 0.018-0.692, P = 0.019). Our data suggest that circulating miR-223 may serve as a novel biomarker for assessment of clopidogrel responsiveness in troponin-negative NSTE-ACS patients. © 2013 Springer Science+Business Media.

Jiang T.,Tianjin University | Jiang T.,Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury | Zhang G.,Tianjin University | He W.,Tianjin University | And 2 more authors.
Journal of Nanomaterials | Year: 2014

Due to the advantage of controllability on the mechanical property and the degradation rates, electrospun PCL/PTMC nanofibrous scaffold could be appropriate for vascular tissue engineering. However, the tissue response and degradation of electrospun PCL/PTMC scaffold in vivo have never been evaluated in detail. So, electrospun PCL/PTMC scaffolds with different blend ratios were prepared in this study. Mice subcutaneous implantation showed that the continuous degradation of PCL/PTMC scaffolds induced a lasted macrophage-mediated foreign body reaction, which could be in favor of the tissue regeneration in graft. © 2014 Tao Jiang et al.

PubMed | Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Tianjin University, Tianjin Medical University and Chinese People's Armed Police forces Academy
Type: | Journal: Journal of molecular and cellular cardiology | Year: 2015

The differentiation of endothelial progenitor cells (EPCs) plays a pivotal role in endothelial repair and re-endothelialization after vascular injury. However, the underlying mechanisms still remain largely elusive. Here, we investigated the role of the novel C2H2 zinc finger transcription factor ZFP580 in EPC differentiation and the molecular mechanisms behind EPC-mediated endothelial repair.Bone marrow-derived EPCs were isolated, cultured, and identified. EPCs were infected with an adenovirus encoding ZFP580 or Ad-siRNA to silence ZFP580. Fluorescence-activated cell sorting (FACS) analysis was performed to analyze EPC surface makers. The expression of ZFP580, eNOS, VEGFR-2, CD31, CD34, CD45 and vWF was performed by Q-PCR, Western blot and immunostaining. NO donor SNAP or NOS inhibitor L-NAME was used to elucidate the possible molecular mechanism. Tube formation in vitro and angiogenesis assay in vivo were also used in this study.Both ZFP580 and eNOS were displayed dynamic expression during EPC differentiation. Overexpression of ZFP580 enhanced EPC differentiation, while knockdown suppressed it. ZFP580 also enhanced eNOS expression, and eNOS inhibition suppressed differentiation. Upregulation/knockdown of ZFP580 also enhanced/reduced endothelial tube formation from EPC in vitro, and angiogenesis in vivo in response to Matrigel plugs containing EPC.ZFP580 promotes not only the differentiation of EPCs into ECs by increasing the expression of eNOS and the availability of nitric oxide, but also the vessel formation in vitro and in vivo. This might represent a novel mechanism of ZFP580 in EPC differentiation and its therapeutic value in the treatment of vascular disease.

PubMed | Tianjin University, Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury and Central University of Costa Rica
Type: | Journal: Journal of proteomics | Year: 2015

Hypoxic status alters the energy metabolism and induces cell injury in cardiomyocytes, and it further triggers the occurrence and development of cardiovascular diseases. Our previous studies have shown that salidroside (SAL) exhibits anti-hypoxic activity. However, the mechanisms remain obscure. In the present study, we successfully screened 92 different expression proteins in CoCl2-induced hypoxic conditions, 106 different expression proteins in the SAL-mediated anti-hypoxic group were compared with the hypoxic group using quantitative proteomics strategy, respectively. We confirmed that SAL showed a positive protective function involving the acetyl-CoA metabolic, tricarboxylic acid (TCA) cycle using bioinformatics analysis. We also demonstrated that SAL plays a critical role in restoring the TCA cycle and in protecting cardiomyocytes from oxidative injury via up-regulation expressions of PDHE1-B, ACO2, SUCLG1, SUCLG2 and down-regulation of MDH2. SAL also inhibited H9c2 cell apoptosis by inhibiting the activation of pro-apoptotic molecules caspase 3 and caspase 9 as well as activation of the anti-apoptotic molecular Bcl-2. Additionally, SAL also improved mitochondrial membrane potential (m), reduced reactive oxygen species (ROS) and intercellular Ca(2+) concentration ([Ca(2+)]i) accumulation and inhibited the excessive consumption of ATP in H9c2 cells.

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