Key Laboratory of Artificial Cells of Tianjin

Tianjin, China

Key Laboratory of Artificial Cells of Tianjin

Tianjin, China

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Gao W.-g.,Tianjin Medical University | Li T.,Tianjin Third Central Hospital | Yu M.-l.,Key Laboratory of Artificial Cells of Tianjin
Chinese Journal of Tissue Engineering Research | Year: 2012

BACKGROUND: For the past few years, the application of heparin coating technology is an active field in biomedical materials. Coating methods, coating models and coating materials are in great development. OBJECTIVE: To summarize the investigative situation and progression about different coating models in accordance with different methods and protection of heparin active groups. METHODS: A computer-based research was performed in CNKI database, PubMed database and US patent database to search the related articles about the application of heparin coating technology in biomedical materials. The key words were "heparin, coating technology, bond method, medical materials, anticoagulation activity" in Chinese and English. The articles related to the heparin coating technology were selected, and articles in the same field that published recently or in authorized journals were preferred. There were 161 articles after the initial survey. Then 29 articles related to heparin coating technology were involved according to the inclusion criteria. RESULTS AND CONCLUSION: Heparin coating technology can simulate the anticoagulant mechanism of vascular endothelial cells, and improve the biocompatibility and anticoagulation activity through modification of biomedical materials surface. Heparin coating technology will make great development and application by increasing the stability and quantity of bonded heparin and retaining heparin activity on biomaterial surface.


Gao W.-Q.,The Third Central Hospital of Tianjin | Gao W.-Q.,Tianjin Medical University | Li T.,The Third Central Hospital of Tianjin | Yu M.-L.,Key Laboratory of Artificial Cells of Tianjin | And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Preliminary experiments have demonstrated that composite coating of sodium alginate and heparin with multi-aldehyde groups has excellent biocompatibility and hemocompatibility. However, little is know about the optimal condition and pattern of new coating preparation. Objective: To prepare a new composite coating of sodium alginate and heparin, and to optimize the coating preparation conditions. Methods: The optimal coating conditions of sodium alginate (or heparin) were screened by orthogonal experiment, to obtain three coating patterns of sodium alginate/heparin composite. Composite coating was covalently bonded on the polyvinyl chloride surface which was preprocessed by amination/chemical modification. The screening methods of optimal composite coating pattern included coating quantification, protein adhesion test and surface contact angle. Anticoagulation properties of optimum composite coating model were assessed. Results and Conclusion: The optimum coating condition of oxidated sodium alginate in composite coating was 50% concentrated sulphuric acid, 0.05% polyethyleneimine, pH 3.5, reaction temperature 40°C and 2 g/L oxidated sodium alginate. The optimum coating condition of heparin in composite coating was 70% concentrated sulphuric acid, 0.1% polyethyleneimine, pH 3.5, reaction temperature 30 °C and 0.1 g/L low molecular nitrous acid-converted heparin. The quantities of oxidated sodium alginate and heparin in optimum composite coating pattern were (4.07±1.35) and (2.13±0.24) μg/cm2. And the composite coating possesses fine anticoagulant property and biocompatibility.


Gao W.-Q.,Third Central Hospital of Tianjin | Li T.,Third Central Hospital of Tianjin | Yu M.-L.,Key Laboratory of Artificial Cells of Tianjin | Hu X.-M.,Third Central Hospital of Tianjin | Duan D.-W.,Third Central Hospital of Tianjin
Chinese Journal of Biomedical Engineering | Year: 2014

The aim of this work is to prepare a novel composite coating of sodium alginate and dexamethasone. By optimizing the coating mode and conditions, the properties of stability, anticoagulation and controlled release were evaluated. Polyethyleneimine (PEI) was coated on PVC pipelines surface pretreated with acidification, dexamethasone sodium phosphate (DSP) was coated on PEI-coated pipelines (PP) with ionic bond, DSP coated pipelines were created (Group PPD). Pipelines coated OSA-DXM composite were created by ionic bond (Group PPI) or covalent bond (Group PPC). The control group (Group C) was set as well. The optimum coating condition was obtained by the orthogonal test. By evaluating the properties of different coated pipelines including anticoagulation, platelet adhesion, protein adhesion and release in vitro, the optimum coating mode was determined. DSP can be bound to PVC surface in Group PPD, PPI and PPC. The optimum immobilization quantity of DSP on different groups are (3. 33 ± 0. 75), (1. 63 ± 0. 76), (2. 06 ±0. 68) μg/cm2. The platelet adhesion (lO9/L) of Group PPI and PPC (13. 88 ± 1. 89 and 19. 13 ± 3. 40) was significantly reduced than that of Group C (41. 38 ± 3. 20). The protein adhesion (mg/cm2) of Group PPI and PPC (HAS: 29. 86 ± 13. 57 and 46. 67 ± 3. 20, HPF: 34. 99 ± 3. 52 and 45. 67 ± 3. 79) was significantly decreased than that of Group PPD (Has: 68. 73 ± 4. 26, HPF: 72. 54 ± 7. 90). The release property of Group PPC is superior to that of Group PPI. The coating of OSA-DXM composite exhibited improved hemocompatibility. Covalently bound DSP can be controlled released.

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