Tianjin Key Laboratory of Aquatic Science and Technology

Tianjin, China

Tianjin Key Laboratory of Aquatic Science and Technology

Tianjin, China
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Li F.,Tianjin University | Zheng J.,Tianjin University | Zheng J.,Tianjin Key Laboratory of Aquatic Science and Technology | Jia Z.,Tianjin University | And 2 more authors.
Chinese Journal of Environmental Engineering | Year: 2017

Simulative tests were conducted in order to study four typical coexisting algae (Microcystis aeruginosa, Chlorella vulgaris, Oocystis sp., and Phormidium.), the response of growth law and the kinetics under different nitrogen and phosphorus nutrient conditions. The Haihe River in Tianjin was considered as the research object. The results showed that (1) a N/P ratio within 10-40 allowed faster algal growth that was particularly vigorous once the N/P ratio equaled 10; (2) the algal growth-rate increased with the increase in N or P concentrations (CN 2.0-15 mg·L-1 and CP 0.2-1.5 mg·L-1), and it reached an optimal value when the abundance of N and P reached 15 and 1.5 mg·L-1 respectively; (3) Oocystis sp. was the dominant species; (4) Monod kinetic analysis revealed that the maximal increment rate was 0.0508 and the semi saturation-constant was 0.157, indicating a confining role of phosphorus on algal growth in the target river. © 2017, Science Press. All right reserved.

Ma X.,Tianjin University | Zhang X.,Tianjin Chengjian University | Zhang X.,Tianjin Key Laboratory of Aquatic Science and Technology | Wang B.,Tianjin University | And 4 more authors.
Evolutionary Applications | Year: 2014

Evolution has resulted in thousands of species possessing similar metabolic enzymes with identical functions that are, however, regulated by different mechanisms. It is thus difficult to select optimal gene to engineer novel or manipulated metabolic pathways. Here, we tested the ability of molecular evolutionary analysis to identify appropriate genes from various species. We calculated the fraction of synonymous substitution and the effective number of codons (ENC) for nine genes stemming from glycolysis. Our research indicated that an enzyme gene with a stronger selective constraint in synonymous sites would mainly regulate corresponding reaction flux through altering the concentration of the protein, whereas those with a more relaxed selective constraint would primarily affect corresponding reaction flux by changing kinetic properties of the enzyme. Further, molecular evolutionary analysis was investigated for three types of genes involved in succinate precursor supply by catalysis of pyruvate. In this model, overexpression of Corynebacterium glutamicum pyc should result in greater conversion of pyruvate. Succinate yields in two Escherichia coli strains that overexpressed each of the three types of genes supported the molecular evolutionary analysis. This approach may thus provide an alternative strategy for selecting genes from different species for metabolic engineering and synthetic biology. © 2014 The Authors.

Liu Y.,CAS Research Center for Eco Environmental Sciences | Liu Y.,Tohoku University | Liu Y.,Tianjin Key Laboratory of Aquatic Science and Technology | Niu Q.,Shandong University | And 7 more authors.
Bioresource Technology | Year: 2017

A novel single-stage partial nitritation–anammox process equipped with porous functional suspended carriers was developed at 25 °C in a CSTR by controlling dissolved oxygen <0.3 mg/L. The nitrogen removal performance was almost unchanged over a nitrogen loading rate ranging from 0.5 to 2.5 kg NH4 +-N/m3/d with a high nitrogen removal efficiency of 81.1%. The specific activity of AOB and anammox bacteria was of 3.00 g-N/g-MLVSS/d (the suspended sludge), 3.56 g-N/g-MLVSS/d (the biofilm sludge), respectively. The results of pyrosequencing revealed that Nitrosomonas (5.66%) and Candidatus_Kuenenia (4.95%) were symbiotic in carriers while Nitrosomonas (40.70%) was predominant in the suspended flocs. Besides, two specific types of heterotrophic filamentous bacteria in the suspended flocs (Haliscomenobacter) and the functional carrier biofilm (Longilinea) were shown to confer structural integrity to the aggregates. The novel single-stage partial nitritation–anammox process equipped with functional suspended carriers was shown to have good potential for the nitrogen-rich wastewater treatment. © 2017 Elsevier Ltd

Wang S.,Tianjin Chengjian University | Wang S.,Tianjin Key Laboratory of Aquatic Science and Technology | Liu Y.,Tohoku University | Liu Y.,CAS Research Center for Eco Environmental Sciences | And 4 more authors.
Bioresource Technology | Year: 2017

The operation performance of a novel micro-granule based syntrophic system of nitritation and anammox was studied by controlling the oxygen concentration and maintaining a constant temperature of 25 °C. With the oxygen concentration of around 0.11 (<0.15) mg/L, the single-stage nitritation-anammox system was startup successfully at a nitrogen loading rate (NLR) of 1.5 kgN/m3/d. The reactor was successfully operated at volumetric N loadings ranging from 0.5 to 2.5 kgN/m3/d with a high nitrogen removal of 82%. The microbial community was composed by ammonia oxidizing bacteria (AOB) and anammox bacteria forming micro-granules with an average diameter of 0.8 mm and good settleability. Results from pyrosequencing analysis revealed that Ca. Kuenenia and Nitrosomonas were selected and enriched in the community over the startup period, and these were identified as the dominant anammox bacteria and AOB species, respectively. © 2017 Elsevier Ltd

Xiao S.,Tianjin Chengjian University | Xiao S.,Tianjin Key Laboratory of Aquatic Science and Technology | Yin P.,Tianjin Chengjian University | Zhang Y.,Tianjin Chengjian University | Hu S.,Nankai University
Korean Journal of Parasitology | Year: 2017

A total of 60 samples were collected from 35 swimming pools in Beijing, China, and the presence of Cryptosporidium and Giardia were investigated. The results showed that 16.7% and 15.0% of samples were positive for Cryptosporidium oocyst and Giardia cysts, respectively, with a mean concentration of 0.30 oocysts/10 L and 0.27 cysts/10 L. The oocysts and cysts were found to have higher rates of occurrence in August than in May. Genotyping confirmed the presence of Cryptosporidium hominis, C. parvum, and Giardia assemblages A and B, all of which were associated with human infections. The predominant species/assemblages were C. hominis and Giardia assemblage A. Analyses of the relationships between parasite oocysts/cysts, indicator bacteria, and physical-chemical parameters revealed that there was no correlation between 2 parasites and fecal bacterial indicators, whilst there was a significant correlation between protozoa and urea concentration, which indicates that urea concentration rather than fecal bacterial indicators might be an appropriate index for chlorine-resistant protozoa in swimming pools. This study provides useful information to improve the safety of swimming pool water and deduce the risk of protozoan infections. © 2017, Korean Society for Parasitology and Tropical Medicine.

Yuan J.,Tianjin Chengjian University | Yuan J.,Tianjin Key Laboratory of Aquatic Science and Technology | Wang B.,Tianjin Chengjian University | Wang B.,Tianjin Key Laboratory of Aquatic Science and Technology | And 2 more authors.
Desalination and Water Treatment | Year: 2017

Based on the present technology in the water plant, the removal of humic acid and nickel have been enhanced by adding coagulant aid polyaluminium chloride (PAC) or potassium ferrate (K2FeO4). The results show that its pH and the dosage of coagulant aid are the two main factors that have effect on the removal of nickel and humic acid (expressed as total organic carbon, TOC). Charge neutralization was the dominant mechanism by PAC, thus incorporating the humic acid and nickel into flocs more efficiently, producing settleable flocs of greater density, size and strength. When the dose of PAC was 40 mg/L at optimum pH 10, the removal efficiency of nickel and TOC reached 95% and 58.7%, respectively. The removal mechanism of complexes by K2FeO4 can be explained by both oxidation and coagulation. Percentage removals of 90 and 61 for nickel and TOC, respectively, were achieved by the addition of 20 mg/L K2FeO4 at optimum pH 8. It is concluded that the enhanced coagulation through the addition of PAC and K2FeO4, as coagulant and oxygenant, is more effective and efficient than traditional coagulation for economic treatment of micro-polluted humic acid and nickel containing surface water. © 2017 Desalination Publications. All rights reserved.

Wang Y.,Tianjin Key Laboratory of Aquatic Science and Technology | Xiong Z.-H.,Tianjin Key Laboratory of Aquatic Science and Technology | Zhou J.-G.,Tianjin Institute of Urban Construction
Zhongguo Huanjing Kexue/China Environmental Science | Year: 2012

The calyx[4]arene was synthesized and connected to Amberlite XAD-4 resin covalently through a diazotization bound. The structure of Amberlite XAD-4 resin base on calyx[4]arene was characterized using FT-IR, SEM and TG/DTA. The sorption results showed that Amberlite XAD-4 resin base on calyx[4]arene had much better removal to the diclofenac in aqueous solutions than Amberlite XAD-4 resin and calyx[4]arene which were presence alone in aqueous solutions. The removal rate of diclofenac increased rapidly with the calyx[4]arene modified Amberlite XAD-4 resin dosage. In the condition of DCF concentration was 20 mg/L, when the Amberlite XAD-4 resin base on calyx[4]arene dosage came up to 80 mg/L, the removal rate of diclofenac was 92.8% and reached equilibrium, the corresponding amount adsorbed was 34.02 mg/g. Kinetic analyses were conducted using pseudo first-order and second-order models. The linear correlation coefficients and standard deviations of Langmuir and Freundlich isotherms were determined, and the results revealed that Langmuir and Freundlich isotherm were fitted the experimental data well. The thermodynamic parameters calculated indicated, ΔH and ΔG were negative, which predicated adsorption process of diclofenac on f Amberlite XAD-4 resin base on calyx[4]arene resin was exothermic and spontaneous.

Fan X.,Tianjin Institute of Urban Construction | Fan X.,Tianjin Key Laboratory of Aquatic Science and Technology | Zhang X.,Tianjin Institute of Urban Construction | Zhang X.,Tianjin University
Advanced Materials Research | Year: 2013

Heavy metal leaching limits activated carbon from sewage sludge (referred as ACS) to be used for wastewater treatment. Cr and Cd leaching from ACS exceed the permitted values. The leaching content of Cr and Cd decrease much with depositing ACS with chitosan (CS),below the permitted values. This is mainly due to the bind of Cr and Cd with the much group -NH2 of CS. The effects of initial dye concentration, pH and contact time had been studied. The dye adsorbed can act as a supple complexion agent with increasing initial dye concentration, increase the affinity for Cr and Cd. For Cd, the amount leaching of CS/ACS or ACS remains content with pH.The amount leaching of Cr decreases when the pH from 1 to 5 and then increases above the pH of 6.0. The contact time has no important influence on the leaching contents of Cr or Cd. © (2013) Trans Tech Publications, Switzerland.

Fan X.D.,Tianjin University | Fan X.D.,Tianjin Key Laboratory of Aquatic Science and Technology | Zhang X.K.,Tianjin University
Environmental Protection and Sustainable Ecological Development - Proceedings of the Environmental Protection and Sustainable Ecological Development, EPSED 2014 | Year: 2015

Norfloxacin (Nor) as a common representative of antibiotics was examined for its degradation by Biological Activated Carbon (BAC). The objectives of this study are to show the performance of BAC process treating Nor from aqueous solutions. The adsorption and biodegradation in BAC reactor (BAC column A) and the other reactor with new carbon, not inoculated (BAC column B) were carried out. The results showed that immediate breakthrough of norfloxacin (Nor) is observed in the effluent of BAC column B. The Nor removal efficiencies increase with hydraulic retention time in BAC column A. When hydraulic retention time is 2 h, Nor removal efficiency reached 77.26%. FTIR results indicate that the benzene ligand of Nor has been destructed and produced free-OH group, the stretch of O-H and N-H decrease with the peaks at 3000–3500 cm−1 shifting to 3500–3600 cm−1. Biodegradation can play the leading pole for Nor removal in BAC reactors. © 2015 Taylor & Francis Group, London.

Fan X.D.,Tianjin University | Fan X.D.,Tianjin Key Laboratory of Aquatic Science and Technology | Zhang X.K.,Tianjin University
Journal of Residuals Science and Technology | Year: 2015

Food waste residue is an intractable problem worldwide. The current study discussed the potential of adsorbents prepared from food waste residue (AFWR) for heavy metal removal from wastewater. The microstructure of AFWR was characterized, and kinetic studies on the simultaneous adsorption of Pb2+, Cu2+, Zn2+ and Cd2+ ions from aqueous solutions by AFWR were carried out. It was found that the adsorption of all heavy metals followed a pseudo-second-order kinetics, so that there was a competitive adsorption for Pb2+, Cu2+, Zn2+ and Cd2+. According to the experimental results, the adsorption in competition beads with the heavy metals presented an order of Cu2+ > Pb2+ > Zn2+ > Cd2+. © 2015 DEStech Publications, Inc.

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