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Zhao S.,Tianjin Normal University | Jia L.,Tianjin Normal University | Duo L.,Tianjin Normal University | Duo L.,Tianjin Key Laboratory of Animal and Plant Resistance
Bioresource Technology | Year: 2013

In a column experiment with horizontal permeable barriers, the effects of a biodegradable chelator-nitrilotriacetic acid (NTA) on the uptake of heavy metals from municipal solid waste (MSW) compost by Festuca arundinacea and metal leaching were investigated. The use of NTA was effective in increasing Cu, Pb, and Zn uptakes in shoots of two crops of F. arundinacea. In columns with barriers and treated with 20. mmol NTA per kg MSW compost, metal uptakes by the first and second crop of F. arundinacea were, respectively, 3.8 and 4.0. times for Pb, and 1.8 and 1.7. times for Zn greater with the added NTA than without it. Though NTA application mobilized metals, it caused only slight leaching of metals from MSW compost. Permeable barriers positioned between compost and soil effectively reduced metal leaching. NTA-assisted phytoextraction by turfgrass with permeable barriers to cleanup heavy metal contaminated MSW compost should be environmentally safe. © 2012 Elsevier Ltd. Source

Liu L.,Tianjin Normal University | Liu L.,Tianjin Key Laboratory of Animal and Plant Resistance | Du W.,Tianjin Normal University | Luo W.,Tianjin Normal University | And 3 more authors.
Molecular Biotechnology | Year: 2015

Phosphorus (P) is one of the most important nutrient elements for plant growth and metabolism. We previously isolated a P-solubilizing bacterium 9320-SD with the ability to utilize inorganic P and convert it into plant-available P. The present study aims to enhance the P-solubilizing capacity of 9320-SD, as our long-term goal is to develop a more effective P-solubilizing bacterial strain for use as a biofertilizer. In this end, we introduced a bacterial phytase encoding gene into 9320-SD. One randomly selected transformant, SDLiuTP02, was examined for recombinant protein expression and phytase activity, and assessed for its ability to promote plant growth. Our results indicate that SDLiuTP02 is capable of expressing high levels of phytase activity. Importantly, corn seedlings treated with the SDLiuTP02 cell culture exhibited increased rates of photosynthesis, transpiration, and stomatal conductance as well as increased growth rate under laboratory conditions and increased growth rate in pot assays compared to seedlings treated with cell cultures of the parental strain 9320-SD. Field experiments further indicated that application of SDLiuTP02 promoted a greater growth rate in young cucumber plant and a higher foliar chlorophyll level in chop suey greens when compared to 9320-SD treated controls. These results indicate that SDLiuTP02 has the potential to be a more effective P biofertilizer to increase agricultural productivity. © 2015, Springer Science+Business Media New York. Source

Zhang G.,Tianjin Normal University | Zhang G.,Tianjin Key Laboratory of Animal and Plant Resistance | Li X.,Tianjin Agricultural University | Kang Y.,Kochi University | And 3 more authors.
Journal of Food, Agriculture and Environment | Year: 2013

Grassland degradation is commonly associated with changes in soil structure, yet variability in soil structure has rarely been studied in grassland ecosystems. Soil aggregation is a key indicator of soil structure and is correlated with erodibility and water infiltration capacity. The objective of this study was to investigate the influences of grassland degradation on soil aggregate properties in Inner Mongolia, China. R0.25, MWD, WSAP and D were determined to characterize the size distribution of soil aggregates and their stability. Grassland degradation resulted in the breakdown of soil aggregates and decreased soil aggregate stability. R0.25, MWD, WSAP and D tended to decrease with progressing grassland degradation. A negative and high correlation existed between soil bulk density and the evaluation indexes (R0.25, MWD, WSAP and D) of soil aggregate stability. There was also a strong positive correlation between soil organic carbon content and the evaluation indexes. Our findings are in agreement with the notion that soil bulk density and soil organic carbon are the primary factors influencing the size and stability of aggregates. Source

Jiang S.,CAS Qingdao Institute of Oceanology | Li H.,CAS Qingdao Institute of Oceanology | Zhang D.,CAS Qingdao Institute of Oceanology | Zhang H.,CAS Qingdao Institute of Oceanology | And 3 more authors.
Fish and Shellfish Immunology | Year: 2015

C1q proteins serve as pattern recognition receptors and involve in the pathogen recognition and complement pathway activation. In the present study, a novel C1q domain containing protein from Crassostrea gigas (designated CgC1qDC-1) was isolated by liposaccharide-Sepharose 6B affinity chromatography. The coding sequence of CgC1qDC-1 gene was determined by performing a homologous search of eight tryptic peptides identified by MALDI-TOF/TOF-MS against the genome of C.gigas. The coding sequence of CgC1qDC-1 was of 387bp encoding a polypeptide of 128 amino acids containing a typical globular C1q domain. The globular C1q domain possessed eight β strands with a jelly-roll topology structure, which was similar to the structure of human gC1q domain. The mRNA transcripts of CgC1qDC-1 were dominantly expressed in mantle and hemocytes, while low expressed in hepatopancreas, gonad, gill and muscle. The expression level of CgC1qDC-1 increased drastically at 6h after Vibrio splendidus stimulation, and then gradually fell to the normal level at about 24h. ELISA assay quantified that CgC1qDC-1 bound to LPS with high binding affinity (Kd=0.09×10-6M). Moreover, CgC1qDC-1 significantly enhanced the phagocytosis of oyster hemocytes towards Gram-negative bacteria Escherichia coli and V. splendidus. These results collectively indicated that CgC1qDC-1 could serve as pattern recognition receptor and opsonin in the innate immune response against invading Gram-negative bacteria. © 2015 Elsevier Ltd. Source

Zhao S.L.,Tianjin Key Laboratory of Animal and Plant Resistance | Zhao S.L.,Tianjin Normal University | Shang X.J.,Tianjin Key Laboratory of Animal and Plant Resistance | Shang X.J.,Tianjin Normal University | And 2 more authors.
International Journal of Environmental Science and Technology | Year: 2013

In a municipal solid waste (MSW) compost field, Kochia scoparia, an easy-to-grow weed plant, gradually invaded the experiment site and became the dominant species after 4 years’ succession. Ethylenediaminetetraacetic acid (EDTA) solution at five rates 0, 25, 50 mmol L−1, 25 mmol L−1 + 1 g L−1 ammonium sulfate (NH4)2SO4, and 50 mmol L−1 + 1 g L−1 (NH4)2SO4 was added to the tested plant root medium. The effects of EDTA and (NH4)2SO4 on Pb and Cr distribution in K. scoparia were investigated. Results suggested that plant biomass increased greatly with height, showing an “inversion pyramid” pattern in spatial structure. At the level of 50 mmol L−1 EDTA, single additions and combined additions with (NH4)2SO4 increased Pb and Cr concentrations in plant shoots at different heights. Lead and Cr uptakes increased toward the top of the shoot. Combined application of 50 mmol L−1 EDTA and (NH4)2SO4 increased Pb uptakes by 21.6, 19.2, 111.3, 124.3, and 154.0 % in 0–30, 30–60, 60–90, 90–120, and over 120 cm spatial shoots, respectively, as compared to those of controls. The increment for Cr uptake was 244.5, 281.7, 100.0, 77.2, and 187.4 %. The relationship between Pb and Cr concentrations in plant shoots and spatial height was found to be positively linear and statistically significant at 1 % level at 50 mmol L−1 EDTA alone and 25 mmol L−1 EDTA together with (NH4)2SO4. Results presented here indicated that K. scoparia had potential in removal of Pb and Cr from MSW compost with the combined application of EDTA and (NH4)2SO4. © 2013, Islamic Azad University (IAU). Source

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