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Zacharogianni M.,Cell Microscopy Center | Zacharogianni M.,Hubrecht Institute for Developmental Biology and Stem Cell Research | Kondylis V.,Cell Microscopy Center | Kondylis V.,University of Cologne | And 10 more authors.
EMBO Journal | Year: 2011

RNAi screening for kinases regulating the functional organization of the early secretory pathway in Drosophila S2 cells has identified the atypical Mitotic-Associated Protein Kinase (MAPK) Extracellularly regulated kinase 7 (ERK7) as a new modulator. We found that ERK7 negatively regulates secretion in response to serum and amino-acid starvation, in both Drosophila and human cells. Under these conditions, ERK7 turnover through the proteasome is inhibited, and the resulting higher levels of this kinase lead to a modification in a site within the C-terminus of Sec16, a key ER exit site component. This post-translational modification elicits the cytoplasmic dispersion of Sec16 and the consequent disassembly of the ER exit sites, which in turn results in protein secretion inhibition. We found that ER exit site disassembly upon starvation is TOR complex 1 (TORC1) independent, showing that under nutrient stress conditions, cell growth is not only inhibited at the transcriptional and translational levels, but also independently at the level of secretion by inhibiting the membrane flow through the early secretory pathway. These results reveal the existence of new signalling circuits participating in the complex regulation of cell growth. © 2011 European Molecular Biology Organization. Source


Zhao M.L.,Tianjin Normal University | Li D.J.,Tianjin Normal University | Gu H.Q.,Tianjin Institute of Urological Surgery | Guo M.X.,Tianjin Normal University | Zhang Y.T.,Tianjin Normal University
Current Nanoscience | Year: 2012

Multi-walled carbon nanotubes (MWCNTs) prepared by chemical vapor deposition (CVD) were sprayed on C wafers. Then, an 8 nm-thick CNx coating was deposited on MWCNTs by ion beam deposition (IBAD). The cell adhesion and hemocompatibility of MWCNTs with and without CNx coating were investigated using contact-angle measurements, cell- and platelet-adhesion assays, and hemolytic-rate testing. The results showed that mouse fibroblast cells (L929) cultured on MWCNTs with CNx coating displayed a higher cell-adhesion strength, viability, proliferation, and spreading than those on MWCNTs without CNx coating, indicating that CNx coating induced MWCNTs showed an increase in cytocompatibility. Despite MWCNTs with CNx coating displayed higher hemolytic and platelet adsorption rates than those on MWCNTs without CNx coating, its hemolytic rate values below 5% were acceptable. And CNx coating inhibited red blood cell adhesion and deformation. Good cell growth and hemocompatibility on MWCNTs with CNx coating were probably related to the positive contribution of nitrogen functional groups to hydrophilicity and the three-dimensional network configuration. © 2012 Bentham Science Publishers. Source


Wang H.Y.,Tianjin Normal University | Wang N.,Tianjin Normal University | Zhang S.,Tianjin Normal University | Deng X.Y.,Tianjin Normal University | And 3 more authors.
Surface and Coatings Technology | Year: 2013

Based on Density Functional Theory (DFT), using first-principles pseudopotential plane wave method, surface relaxation, surface energy and Density of states (DOS) of metal W on low index surfaces (001), (110) and (111) were calculated. The results showed that: the relaxation and surface energy were |δ14(110)|<|δ14(111)|<|δ14(001)| and Esurf(110) Source


Wang H.T.,Tianjin Medical University | Wang H.T.,Research Group of Evidence based Clinical Oncology | Wang H.T.,Tianjin Key Laboratory of Cancer Prevention and Therapy | Li B.G.,Tianjin Medical University | And 5 more authors.
Tumor Biology | Year: 2014

Liver metastasis fromprostate cancer is uncommon and remains poorly understood. We computer searched the clinical records of all our patients registered into a database to identify patients that presented or developed liver metastases. A total of 27 prostate cancer patients with ultrasound or CT/MRimaging evidence of livermetastases were included in our analysis. The liver metastasis rate from metastatic prostate cancer was 4.29 %. Eight (29.63 %) patients had previously untreated, hormone-naive prostate cancer (synchronous liver metastases at diagnosis of prostate cancer), whereas 19 (70.37 %) patients had already been diagnosed as having hormone-refractory prostate cancer. In the hormone-naive group, the median overall survival after liver metastases diagnosis was 38 months and half of the patients were still alive at the latest follow-up, whereas only 6 months in the hormonerefractory group (p =0.003). High concentration of serum neuron-specific enolase and previous chemotherapy were associated with a significantly poor overall survival after liver metastases in the hormone-refractory group using Kaplan-Meier curves and logrank tests for univariate analysis. Source


Yu S.,University of Rochester | Yu S.,Shanghai JiaoTong University | Zhang C.,University of Rochester | Lin C.-C.,University of Rochester | And 10 more authors.
Prostate | Year: 2011

Background Androgens and the androgen receptor (AR) play critical roles in the prostate development via mesenchymal-epithelial interactions. Smooth muscle cells (SMC), differentiated from mesenchyme, are one of the basic components of the prostate stroma. However, the roles of smooth muscle AR in prostate development are still obscure. Methods We established the smooth muscle selective AR knockout (SM-ARKO) mouse model using the Cre-loxP system, and confirmed the ARKO efficiency at RNA, DNA and protein levels. Then, we observed the prostate morphology changes, and determined the epithelial proliferation, apoptosis, and differentiation. We also knocked down the AR in a prostate smooth muscle cell line (PS-1) to confirm the in vivo findings and to probe the mechanism. Results The AR was selectively and efficiently knocked out in the anterior prostates of SM-ARKO mouse. The SM-ARKO prostates have defects with loss of infolding structures, and decrease of epithelial proliferation, but with little change of apoptosis and differentiation. The mechanism studies showed that IGF-1 expression level decreased in the SM-ARKO prostates and AR-knockdown PS-1 cells. The decreased IGF-1 expression might contribute to the defective development of SM-ARKO prostates. ConclusionS The AR in SMCs plays important roles in the prostate development via the regulation of IGF-1 signal. Prostate 77:517-524, 2011. © 2010 Wiley-Liss, Inc. Source

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