Tianjin Institute of Urological Surgery

Tianjin, China

Tianjin Institute of Urological Surgery

Tianjin, China
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Wang H.Y.,Tianjin Normal University | Wang N.,Tianjin Normal University | Zhang S.,Tianjin Normal University | Deng X.Y.,Tianjin Normal University | And 3 more authors.
Surface and Coatings Technology | Year: 2013

Based on Density Functional Theory (DFT), using first-principles pseudopotential plane wave method, surface relaxation, surface energy and Density of states (DOS) of metal W on low index surfaces (001), (110) and (111) were calculated. The results showed that: the relaxation and surface energy were |δ14(110)|<|δ14(111)|<|δ14(001)| and Esurf(110)

Zhang Y.,Tianjin Normal University | Li D.,Tianjin Normal University | Zhao M.,Tianjin Normal University | Guo M.,Tianjin Normal University | And 3 more authors.
Functional Materials Letters | Year: 2013

Influence of carboxylic functionalization on the cytocompatibility of multiwalled carbon nanotubes (MWCNTs) was investigated in this work. Water contact angle assay showed that the surface of MWCNTs-containing carboxyl (MWCNTs-COOH) became much more hydrophilic compared with pure MWCNTs. In cell-adhesion assays, two cell lines, mouse fibroblast cells (L929) and human umbilical vein endothelial cells (EAHY926) were used to assess the cytocompatibility of materials. The MWCNTs-COOH displayed the improved cell proliferation, viability and adhesion due to the enhanced wettability, indicating their superior cytocompatibility over MWCNTs. The existence of carboxyl groups should be benefit to the adhesion and growth of both cells, which implied that MWCNTs-COOH were helpful for seeding both cells and could be used as the functional surface for the adhesion and growth of cells. © 2013 World Scientific Publishing Company.

PubMed | University of Western Ontario, Tianjin Normal University and Tianjin Institute of Urological Surgery
Type: | Journal: Scientific reports | Year: 2016

Low hydrophilicity of graphene is one of the major obstacles for biomaterials application. To create some hydrophilic groups on graphene is addressed this issue. Herein, COOH

Wang C.,University of Toronto | Wang C.,Tianjin Institute of Urological Surgery | Tao W.,Harbin Medical University | Wang Y.,University of Toronto | And 9 more authors.
European Urology | Year: 2010

Background: Prostate cancer (PCa) is the most common malignancy in males in Western countries. Despite improvements in standard treatments such as surgery, radiotherapy, and chemotherapy, many patients still progress to advanced stages. Recent clinical trials have shown encouraging results regarding the application of angiogenic inhibitors in the treatment of angiogenesis-dependent diseases, paving the way for novel PCa therapies. Objective: To identify new antiangiogenic compounds and examine their therapeutic potential in models of PCa. Design, setting, and participants: We performed a chemical genetic screen in developing zebrafish embryos to identify small molecules inhibiting zebrafish angiogenesis. Transgenic Tg(flk1:EGFP) zebrafish embryos were used in the screening of the Spectrum Collection compound library. Subsequently, the antiangiogenic mechanism of an identified lead compound, rosuvastatin, was studied by conducting endothelial cell function assays and examining antitumor efficacy in a PCa xenograft mouse model. Measurements, results and limitations: Seven lead compounds, including isorotenone, dihydromunduletone, aristolochic acid, simvastatin, mevastatin, lovastatin, and rosuvastatin, were identified to inhibit the growth of the zebrafish intersegmental vessels. Of these seven leads, rosuvastatin was further evaluated for its antiangiogenic mechanism and anticancer efficacy. Rosuvastatin decreased the viability of the human umbilical endothelial cells (HUVECs) (one-half inhibitory concentration: 5.87 μM) by inducing G1 phase arrest and promoting apoptosis. Moreover, rosuvastatin remarkably inhibited the migration of HUVECs and dose-dependently inhibited the HUVEC capillary-like tube formation in vitro. Furthermore, we demonstrated that rosuvastatin suppressed xenografted PPC-1 prostate tumors in nonobese diabetic severe combined immunodeficiency (NOD-SCID) mice associated with decreased microvessel density (MVD) and tumor cell apoptosis. Conclusions: Collectively, our data suggest that rosuvastatin possesses antiangiogenic and antitumor activities and has therapeutic potential for the treatment of PCa. This study represents the first zebrafish antiangiogenic chemical genetic screen to identify a lead compound that targets cancer angiogenesis. © 2010 European Association of Urology. Published by Elsevier B.V. All rights reserved.

Zacharogianni M.,Cell Microscopy Center | Zacharogianni M.,Hubrecht Institute for Developmental Biology and Stem Cell Research | Kondylis V.,Cell Microscopy Center | Kondylis V.,University of Cologne | And 10 more authors.
EMBO Journal | Year: 2011

RNAi screening for kinases regulating the functional organization of the early secretory pathway in Drosophila S2 cells has identified the atypical Mitotic-Associated Protein Kinase (MAPK) Extracellularly regulated kinase 7 (ERK7) as a new modulator. We found that ERK7 negatively regulates secretion in response to serum and amino-acid starvation, in both Drosophila and human cells. Under these conditions, ERK7 turnover through the proteasome is inhibited, and the resulting higher levels of this kinase lead to a modification in a site within the C-terminus of Sec16, a key ER exit site component. This post-translational modification elicits the cytoplasmic dispersion of Sec16 and the consequent disassembly of the ER exit sites, which in turn results in protein secretion inhibition. We found that ER exit site disassembly upon starvation is TOR complex 1 (TORC1) independent, showing that under nutrient stress conditions, cell growth is not only inhibited at the transcriptional and translational levels, but also independently at the level of secretion by inhibiting the membrane flow through the early secretory pathway. These results reveal the existence of new signalling circuits participating in the complex regulation of cell growth. © 2011 European Molecular Biology Organization.

Wang H.T.,Tianjin Medical University | Wang H.T.,Research Group of Evidence based Clinical Oncology | Wang H.T.,Tianjin Key Laboratory of Cancer Prevention and Therapy | Li B.G.,Tianjin Medical University | And 5 more authors.
Tumor Biology | Year: 2014

Liver metastasis fromprostate cancer is uncommon and remains poorly understood. We computer searched the clinical records of all our patients registered into a database to identify patients that presented or developed liver metastases. A total of 27 prostate cancer patients with ultrasound or CT/MRimaging evidence of livermetastases were included in our analysis. The liver metastasis rate from metastatic prostate cancer was 4.29 %. Eight (29.63 %) patients had previously untreated, hormone-naive prostate cancer (synchronous liver metastases at diagnosis of prostate cancer), whereas 19 (70.37 %) patients had already been diagnosed as having hormone-refractory prostate cancer. In the hormone-naive group, the median overall survival after liver metastases diagnosis was 38 months and half of the patients were still alive at the latest follow-up, whereas only 6 months in the hormonerefractory group (p =0.003). High concentration of serum neuron-specific enolase and previous chemotherapy were associated with a significantly poor overall survival after liver metastases in the hormone-refractory group using Kaplan-Meier curves and logrank tests for univariate analysis.

Kondylis V.,Cell microscopy Center | Kondylis V.,University of Cologne | Tang Y.,Cell microscopy Center | Tang Y.,Tianjin Institute of Urological Surgery | And 5 more authors.
PLoS ONE | Year: 2011

Background: In Drosophila, the early secretory apparatus comprises discrete paired Golgi stacks in close proximity to exit sites from the endoplasmic reticulum (tER sites), thus forming tER-Golgi units. Although many components involved in secretion have been identified, the structural components sustaining its organisation are less known. Here we set out to identify novel ER resident proteins involved in the of tER-Golgi unit organisation. Results: To do so, we designed a novel screening strategy combining a bioinformatics pre-selection with an RNAi screen. We first selected 156 proteins exhibiting known or related ER retention/retrieval signals from a list of proteins predicted to have a signal sequence. We then performed a microscopy-based primary and confirmation RNAi screen in Drosophila S2 cells directly scoring the organisation of the tER-Golgi units. We identified 49 hits, most of which leading to an increased number of smaller tER-Golgi units (MG for "more and smaller Golgi") upon depletion. 16 of them were validated and characterised, showing that this phenotype was not due to an inhibition in secretion, a block in G2, or ER stress. Interestingly, the MG phenotype was often accompanied by an increase in the cell volume. Out of 6 proteins, 4 were localised to the ER. Conclusions: This work has identified novel proteins involved in the organisation of the Drosophila early secretory pathway. It contributes to the effort of assigning protein functions to gene annotation in the secretory pathway, and analysis of the MG hits revealed an enrichment of ER proteins. These results suggest a link between ER localisation, aspects of cell metabolism and tER-Golgi structural organisation. © 2011 Kondylis et al.

Wang C.,Tianjin Institute of Urological Surgery | Wang C.,University of Toronto | Tao W.,Harbin Medical University | Chen Q.,Tianjin Institute of Urological Surgery | And 3 more authors.
Prostate | Year: 2010

BACKGROUND. Increasing studies investigating the association between steroid 5-alpha reductase type II gene polymorphism at codon 89 (SRD5A2 V89L) and susceptibility to prostate cancer (PCa) confer inconsistent results. To precisely estimate the relationship with more statistical power, a meta-analysis was performed. METHODS. A comprehensive search was conducted to identify all case-control studies investigating such an association. Odds ratio (OR) and its 95% confidence interval (CI) were used to evaluate the size effect. RESULTS. Twenty-five eligible reports were identified including 8,615 cases/9,089 controls in 33 comparisons. In overall analysis, no significant associations were found in all genetic models. Subgroup analyses by ethnicity revealed that small excess PCa risks were observed in dominant model (OR, 1.11; 95% CI, 1.03-1.19 for (LL + VL) vs. VV; P<0.01; Pheterogeneity=0.49) and L allele frequency comparison (OR, 1.09; 1.03-1.15 for L allele frequency; P<0.01; Pheterogeneity=0.07) in Europeans. Meanwhile, SRD5A2 V89L polymorphism was significantly associated with an increased PCa risk in men aged ≤65 under the co-dominant (OR, 1.70; 95% CI, 1.09-2.66 for LL vs. VV; P=0.02; Pheterogeneity=0.31) and recessive (OR, 1.75; 95% CI, 1.14-2.68 for LL vs. (VV + VL); P=0.01; Pheterogeneity=0.12) models. However, no significant associations were found in Asians and Africans. CONCLUSIONS. Our study suggests SRD5A2 V89L polymorphism could play a lowpenetrant role in PCa risk among Europeans and individuals younger than 65 years. Additional well-designed studies are warranted to validate these findings. © 2009 Wiley-Liss, Inc.

Ni S.,Harbin Medical University | Tao W.,Harbin Medical University | Chen Q.,Harbin Medical University | Liu L.,Harbin Medical University | And 4 more authors.
European Urology | Year: 2012

Context: Laparoscopic nephroureterectomy (LNU) has increasingly been used as a minimally invasive alternative to open nephroureterectomy (ONU), but studies comparing the efficacy and safety of the two surgical procedures are still limited. Objective: Evaluate the oncologic and perioperative outcomes of LNU versus ONU in the treatment of upper urinary tract urothelial carcinoma. Evidence acquisition: A systematic review and cumulative analysis of comparative studies reporting both oncologic and perioperative outcomes of LNU and ONU was performed through a comprehensive search of the Medline, Embase, and the Cochrane Library electronic databases. All analyses were performed using the Review Manager (RevMan) v.5 (Nordic Cochrane Centre, Copenhagen, Denmark) and Meta-analysis In eXcel (MIX) 2.0 Pro (BiostatXL) software packages. Evidence synthesis: Twenty-one eligible studies (1235 cases and 3093 controls) were identified. A significantly higher proportion of pTa/Tis was observed in LNU compared to ONU (27.52% vs 22.59%; p = 0.047), but there were no significant differences in other stages and pathologic grades (all p > 0.05). For patients who underwent LNU, the 5-yr cancer-specific survival (CSS) rate was significantly higher, at 9% (p = 0.03), compared to those who underwent ONU, while the overall recurrence rate and bladder recurrence rate were notably lower, at 15% (p = 0.01) and 17% (p = 0.02), respectively. However, there were no statistically significant differences in 2-yr CSS, 5-yr recurrence-free survival (RFS), 5-yr overall survival (OS), 2-yr OS, and metastasis rates between LNU and ONU (all p > 0.05). Moreover, there were no significant differences between LNU and ONU in terms of intraoperative complications, postoperative complications, and perioperative mortality (all p > 0.05). The results of our study were mainly limited by the retrospective design of most of the individual studies included as well as selection biases based on different management of regional lymph nodes and pathologic characteristics. Conclusions: Our data suggest that LNU offers reliable perioperative safety and comparable oncologic efficacy when compared to ONU. Given that some limitations cannot be overcome, well-designed prospective trials are needed to confirm our findings. © 2012 European Association of Urology.

Cui X.A.,Tianjin Medical University | Liu X.,Tianjin Institute of Urological Surgery | Kong D.L.,Key Laboratory of Bioactive Materials | Gu H.Q.,Tianjin Institute of Urological Surgery
IFMBE Proceedings | Year: 2013

The electrospun collagen (COL)/silk fibroin (SF) complex scaffold was investigated for the fabrication of a biocompatible and biomimetic scaffold for tissue engineering. The COL/SF complex microfibers were prepared via electrospinning COL/SF blend solutions in 1,1,1,3,3,3-hexafluoroiso propanol (HFIP), and then crosslinked by glutaraldehyde (GTA) vapor. The fiber morphology was observed by scanning electron microscopy (SEM) and the structural changes of fibers after crosslinked were analysised by Fourier transform infrared spectra (FTIR). The mechanical property of the scaffolds was examined by tensile testing. To assay the biocompatibility of the matrics, the proliferation of fibroblasts (L929) on the microfibrous scaffolds was investigated by methylthiazol tetrazolium testing (MTT). The results showed that the average diameters of complex fibers ranged from 550 to 1100 nm, increasing with the increase of SF content. The GTA vapor stabilized the microfibers especially the COL component via crosslinking and stabilized the SF component via changing the SF component to β-sheet structure. The mechanical property of the crosslinked fibers was better than that of the uncrosslinked ones, and the highest average ultimate tensile strength 8.7 MPa appeared when SF content was 70%. L929 cells grew and proliferated well on the microfibers, especially on the fibers with SF content of 70%. These results strongly support that the COL/SF microfibrous scaffolds, could be a potential candidate for biomedical applications such as wound dressing and scaffolds for skin tissue engineering. © 2013 Springer-Verlag.

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