PubMed | Tianjin Medical University, Tianjin Huanhu Hospital and Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine
Type: | Journal: Chemico-biological interactions | Year: 2016
Postmenopausal osteoporosis is characterized by a reduction in the number of sinusoidal and arterial capillaries in the bone marrow and reduced bone perfusion. Thus, osteogenesis and angiogenesis are coupled in the process of osteoporosis formation and fracture healing. Naringin is the main ingredient of the root Rhizoma Drynariae, a traditional Chinese medicine, and it has potential effects on promoting fracture healing. However, whether naringin stimulates angiogenesis in the process of bone healing is unclear. Here, we show that naringin promotes fracture healing through stimulating angiogenesis by regulating the VEGF/VEGFR-2 signaling pathway in osteoporotic rats.
Lv J.,Tianjin Medical University |
Lv J.,Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine |
Sun X.,Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine |
Ma J.,Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine |
And 6 more authors.
Biochemical and Biophysical Research Communications | Year: 2015
Schwann cells (SCs) play an essentially supportive role in the regeneration of injured peripheral nerve system (PNS). As Netrin-1 is crucial for the normal development of nervous system (NS) and can direct the process of damaged PNS regeneration, our study was designed to determine the role of Netrin-1 in RSC96 Schwann cells (an immortalized rat Schwann cell line) proliferation and migration. Our studies demonstrated that Netrin-1 had no effect on RSC96 cells proliferation, while significantly promoted RSC96 cells migration. The Netrin-1-induced RSC96 cells migration was significantly attenuated by inhibition of p38 and PI3K through pretreatment with SB203580 and LY294002 respectively, but not inhibition of MEK1/2 and JNK by U0126-EtOH and SP600125 individually. Treatment with Netrin-1 enhanced the phosphorylation of p38 and Akt. QRT-PCR indicated that Netrin-1 and only its receptors Unc5a, Unc5b and Neogenin were expressed in RSC96 cells, among which Unc5b expressed the most. And UNC5B protein was significantly increased after stimulated by Netrin-1. In conclusion, we show here that Netrin-1-enhanced SCs migration is mediated by activating p38 MAPK and PI3K-Akt signal cascades via receptor UNC5B, which suggests that Netrin-1 could serve as a new therapeutic strategy and has potential application value for PNS regeneration. © 2015 Elsevier Inc. All rights reserved.
Ma X.-L.,Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine |
Ma X.-L.,Tianjin Medical University |
Sun X.-L.,Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine |
Du Y.-R.,Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine |
And 6 more authors.
Chinese Traditional and Herbal Drugs | Year: 2013
Objective: Under the microstrain environment, to investigate the impact and mechanisms of osteoblast differentiation of the naringin monomer on marrow stem cells (MSCs) in vitro. Methods: The cyclical stretch microstrain was loaded on silicone rubber membrane with cultured rabbit MSCs using EF3200 mechanical tester with the system of BioDynamic biological reaction tank. The experiment was divided into eight groups, A: the blank control group in conventional cultured environment; B: the group of alone microstrain loading; C: the group cultured in environment containing naringin and the naringin concentrations, respectively were 2, 20, and 200 ng/mL; D: under microstrain environment, the joint application with naringin at concentration of 2, 20, and 200 ng/mL. Flow cytometry was used to test the proliferation index (PI) of MSCs after microstrain loading. The gene expression of the cells in osteocalcin (OCN), osteoblast specific transcription factor (Runx2) and collagen type I (Col I) were assayed by RT-PCR. Results: Under the microstrain environment combined with naringin, MSCs showed obvious polarity and the long axis of cells parallel to the direction of mechanical stimulus direction. The combination of naringin and microstrain stimulation can significantly improve the MSCs proliferation activity. Naringin (200 ng/mL) could improve the gene expression of OCN under the stimulation of 50000 microstrain. Under 50000 microstrain stimulation, naringin at different concentration could significantly enhance the Runx2 gene expression and the effect between the enhancement and the naringin concentration was positive. Under the microstrain stimulation, naringin at low concentration could promote the Col I gene expression, on the contrary, naringin at high concentration could inhibit the Col I gene expression. Conclusion: Under the microstrain environment, naringin monomer could enhance the proliferation of MSCs and promote the osteogenetic differentiation of MSCs.
PubMed | Tianjin Medical University and Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine
Type: Journal Article | Journal: Biochemical and biophysical research communications | Year: 2014
Naringin, the primary active compound of the traditional Chinese medicine Rhizoma drynariae, possesses many pharmacological activities. The present study is an effort to explore the anti-osteoporosis potential of naringin in vivo and in vitro. In vivo, we used ovariectomized rats to clarify the mechanisms by which naringin anti-osteoporosis. In vitro, we used osteoclasts to investigate naringin promotes osteoclasts apoptosis. Naringin was effective at enhancing BMD, trabecular thickness, bone mineralization, and mechanical strength in a dose-dependent manner. The result of RT-PCR analysis revealed that naringin down-regulated the mRNA expression levels of BCL-2 and up-regulated BAX, caspase-3 and cytochrome C. In addition, naringin significantly reduced the bone resorption area in vitro. These findings suggest that naringin promotes the apoptosis of osteoclasts by regulating the activity of the mitochondrial apoptosis pathway and prevents OVX-induced osteoporosis in rats.