Tianjin Institute of Health and Environmental Medicine

Tianjin, China

Tianjin Institute of Health and Environmental Medicine

Tianjin, China
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Huang H.-N.,Central South University | Huang H.-N.,Tianjin Institute of Health and Environmental Medicine | Zhang H.,Tianjin Institute of Health and Environmental Medicine | Wang H.,Tianjin Institute of Health and Environmental Medicine
Chinese Pharmacological Bulletin | Year: 2015

Thalidomide and its analogues (lenalidomide and pomalidomide) are small glutamic acid derivatives with strong immunomodulatory effects, belonging to immunomodulatory drug (IMiD) class. In addition, these thalidomide analogues demonstrate an overlapping and diverse range of biological activities, including anti-angiogenesis and anti-teratogenieity. Importantly, the IMiDs exert anticancer effects such as inhibiting tumor cell proliferation and promoting tumor cell apoptosis and play important roles especially in clinical treatment of multiple myeloma. Recently, the screening and discovery of thalidomide binding protein, CRBN provides new clues to the research of its pharmacological mechanisms and the develop ment of new generation of thalidomide derivatives with less toxic side effects and more antitumor potency. This review briefly discusses the underlying mechanism of thalidomide and their derivatives' action and their new identified target, as well as their contribution to the treatment of multiple myeloma.


PubMed | Tianjin Institute of Health and Environmental Medicine, University of Sichuan and Centers for Disease Control and Prevention
Type: Journal Article | Journal: Nutritional neuroscience | Year: 2016

An intervention study was performed to determine if supplement containing folic acid, vitamin BOne hundred and four participants with hyperhomocysteinemia were recruited in Tianjin, China, aged 55-94 years old. Fifty-seven individuals with hyperhomocysteinemia were included in the intervention group (vitamin B group, which received 800g/day of folate, with 10mg of vitamin BThe BCAT total score and four sub-tests scores (digit copy, Chinese character rotation, digital working memory, and recognition of meaningless figure) of BCAT at 14 weeks significantly increased only for the vitamin B group. Serum total homocysteine (tHcy) levels significantly decreased in the intervention group, while serum concentrations of folate, vitamin BThe results demonstrated that supplement containing folate, vitamin B


Shen Z.,Tianjin Institute of Health and Environmental Medicine | Hou N.,Tianjin Institute of Health and Environmental Medicine | Jin M.,Tianjin Institute of Health and Environmental Medicine | Qiu Z.,Tianjin Institute of Health and Environmental Medicine | And 6 more authors.
Gut Pathogens | Year: 2014

This paper presents a functional nanoparticle-enhanced enzyme-linked immunosorbent assay (FNP-ELISA) for detection of enterohemorrhagic Escherichia coli (EHEC) O157:H7. Immunomagnetic nanoparticles (IMMPs) conjugated with monoclonal anti-O157:H7 antibody were used to capture E. coli O157:H7. Beacon gold nanoparticles (B-GNPs) coated with polyclonal anti-O157:H7 and biotin single-stranded DNA (B-DNA) were then subjective to immunoreaction with E. coli O157:H7, which was followed by streptavidin-horseradish peroxidase (Strep-HRP) conjugated with B-GNPs based on a biotin-avidin system. The solutions containing E. coli O157:H7, IMMPs, B-GNPs, and Strep-HRP were collected for detecting color change. The signal was significantly amplified with detection limits of 68 CFU mL-1 in PBS and 6.8 × 102 to 6.8 × 103 CFU mL-1 in the food samples. The FNP-ELISA method developed in this study was two orders of magnitude more sensitive than immunomagnetic separation ELISA (IMS-ELISA) and four orders of magnitude more sensitive than C-ELISA. The entire detection process of E. coli O157:H7 lasted only 3 h, and thus FNP-ELISA is considered as a time-saving method. © 2014 Shen et al.; licensee BioMed Central Ltd.


PubMed | Tianjin Institute of Health and Environmental Medicine, South China Agricultural University, Henan Institute of Science and Technology, Zhengzhou University and 2 more.
Type: | Journal: Scientific reports | Year: 2016

Bacteriophages have recently been considered as an alternative biocontrol tool because of the widespread occurrence of antimicrobial-resistant Achromobacter xylosoxidans. Herein, we isolated a virulent bacteriophage (phiAxp-1) from a water sample of the Bohai sea of China that specifically infects A. xylosoxidans. Transmission electron microscopy revealed that phage phiAxp-1 belongs to the Siphoviridae. We sequenced the genome of phiAxp-1, which comprises 45,045bp with 64 open reading frames. Most of the proteins encoded by phiAxp-1 have no similarity to sequences in the public databases. Twenty-one proteins with assigned functions share weak homology with those of other dsDNA bacteriophages infecting diverse hosts, such as Burkholderia phage KL1, Pseudomonas phage 73, Pseudomonas phage vB_Pae-Kakheti25, Pseudomonas phage vB_PaeS_SCH_Ab26, Acinetobacter phage IME_AB3 and Achromobacter phage JWX. The genome can be divided into different clusters for the head and tail structure, DNA replication and mazG. The sequence and genomic organization of bacteriophage phiAxp-1 are clearly distinct from other known Siphoviridae phages; therefore, we propose that it is a member of a novel genus of the Siphoviridae family. Furthermore, one-step growth curve and stability studies of the phage were performed, and the specific receptor of phiAxp-1 was identified as the lipopolysaccharide of A. xylosoxidans.


Ma P.,University of Science and Technology of China | Ma P.,Central China Normal University | Luo Q.,Central China Normal University | Chen J.,University of Science and Technology of China | And 5 more authors.
International Journal of Nanomedicine | Year: 2012

Because of its unique magnetic properties, the iron oxide (Fe3O4) nanoparticle has been widely exploited and its application in various fields has promised immense benefits. However, doubts exist over the use of Fe3O4-nanoparticles in human beings. Thus, the aim of the current study was to find out the potential safety range of medical use. Twenty-five Kunming mice were exposed to Fe3O4-nanoparticles via intraperitoneal injection daily for 1 week at doses of 0, 5, 10, 20, and 40 mg/kg. Hepatic and renal tissues were sliced for physiological observation. Injuries were observed in the high-dose groups (20 and 40 mg/kg) compared with the control group (0 mg/kg). Biomarkers of reactive oxygen species, glutathione, malondialdehyde, DNA-protein crosslinks, and 8-hydroxy-2′-deoxyguanosine in the hepatic and renal tissues were detected. Injury to tissues and oxidative damage to cells at the molecular level was found. The safest dose recommended from the results of this study is 5 mg/kg, as we believe this to be an upper limit balancing the benefits and risks for sub-long-term exposure. © 2012 Ma et al, publisher and licensee Dove Medical Press Ltd.


PubMed | Tianjin Institute of Health and Environmental Medicine, University of Minnesota, China Pharmaceutical University, Xuzhou Medical College and 2 more.
Type: Journal Article | Journal: Journal of cellular and molecular medicine | Year: 2016

The development of multidrug resistance (MDR) not only actively transports a wide range of cytotoxic drugs across drug transporters but is also a complex interaction between a number of important cellular signalling pathways. Nitric oxide donors appear to be a new class of anticancer therapeutics for satisfying all the above conditions. Previously, we reported furoxan-based nitric oxide-releasing compounds that exhibited selective antitumour activity in vitro and in vivo. Herein, we demonstrate that bifendate (DDB)-nitric oxide, a synthetic furoxan-based nitric oxide-releasing derivative of bifendate, effectively inhibits the both sensitive and MDR tumour cell viability at a comparatively low concentration. Interestingly, the potency of DDB-nitric oxide is the independent of inhibition of the functions and expressions of three major ABC transporters. The mechanism of DDB-nitric oxide appears to be in two modes of actions by inducing mitochondrial tyrosine nitration and apoptosis, as well as by down-regulating HIF-1 expression and protein kinase B (AKT), extracellular signal-regulated kinases (ERK), nuclear factor B (NF-B) activation in MDR cells. Moreover, the addition of a typical nitric oxide scavenger significantly attenuated all the effects of DDB-nitric oxide, indicating that the cytotoxicity of DDB-nitric oxide is as a result of higher levels of nitric oxide release in MDR cancer cells. Given that acquired MDR to nitric oxide donors is reportedly difficult to achieve and genetically unstable, compound like DDB-nitric oxide may be a new type of therapeutic agent for the treatment of MDR tumours.


Wang S.-Y.,Tianjin Institute of Health and Environmental Medicine | Cui W.-Y.,Thadweik Academy of Medicine | Wang H.,Tianjin Institute of Health and Environmental Medicine
Acta Pharmacologica Sinica | Year: 2015

Aim: To investigate the mechanisms underlying the activation of ATP-sensitive potassium channels (KATP) by iptakalim in cultured rat mesenteric microvascular endothelial cells (MVECs). Methods: Whole-cell KATP currents were recorded in MVECs using automated patch clamp devices. Nucleotides (ATP, ADP and UDP) were added to the internal perfusion system, whereas other drugs were added to the cell suspension on NPC-1 borosilicate glass chips. Results: Application of iptakalim (10 and 100 μmol/L) significantly increased the whole-cell KATP currents, which were prevented by the specific KATP blocker glibenclamide (1.0 μmol/L). The opening of KATP channels by iptakalim depended upon the intracellular concentrations of ATP or NDPs: iptakalim activated KATP channels when the intracellular ATP or NDPs were at 100 or 1000 μmol/L, and was ineffective when the non-hydrolysable ATP analogue ATPγS (1000 μmol/L) was infused into the cells. In contrast, the KATP opener pinacidil activated KATP channels when the intracellular concentrations of ATP or NDPs ranged from 10 to 5000 μmol/L, and even ATPγS (1000 μmol/L) was infused into the cells. Conclusion: Iptakalim activates KATP channels in the endothelial cells of resistance blood vessels with a low metabolic status, and this activation is dependent on both ATP hydrolysis and ATP ligands. © 2015 CPS and SIMM All rights reserved.


PubMed | Tianjin Institute of Health and Environmental Medicine, Northwest University, China, Zhengzhou University, South China Agricultural University and Academy of Military Medical science
Type: Journal Article | Journal: Journal of medical virology | Year: 2016

Citrobacter freundii, a Gram-negative bacterium, causes many opportunistic infections. Bacteriophage phiCFP-1 was isolated and characterized by its ability to lyse the multidrug-resistant clinical C. freundii strain P10159. Transmission electron microscopy showed that the phage has an icosahedral head and a short tail, making it a Podoviridae family member. In a single-step growth experiment, phiCFP-1 exhibited an eclipse period of 20min and a burst size of 100 particles per cell. Its genome assembled as a circular molecule when genomic sequencing was completed. However, based on genome content and organization, it was categorized as a classic T7-related phage, and such phages are known to have linear genomes with direct terminal repeats. With the quick and simple method established herein, the 38,625-bp linear double-stranded DNA with 229-bp direct terminal repeats was accurately identified. The genome contained 43 putative open reading frames and no tRNA genes. Using a proteomics-based approach, seven viral and two host proteins from purified phiCFP-1 particles were identified. Comparative genomics and recombination analyzes revealed close genetic relatedness among phiCFP-1, phiYeO3-12/vB_YenP_AP5 (from Yersinia enterocolitica O3), and phiSG-JL2 (from Salmonella enterica).


PubMed | Tianjin Institute of Health and Environmental Medicine
Type: Journal Article | Journal: Neurochemical research | Year: 2016

Sleep disorders negatively affect cognition and health. Recent evidence has indicated that chromatin remodeling via histone acetylation regulates cognitive function. This study aimed to investigate the possible roles of histone acetylation in sleep deprivation (SD)-induced cognitive impairment. Results of the Morris water maze test showed that 3days of SD can cause spatial memory impairment in Wistar rats. SD can also decrease histone acetylation levels, increase histone deacetylase 2 (HDAC2) expression, and decrease histone acetyltransferase (CBP) expression. Furthermore, SD can reduce H3 and H4 acetylation levels in the promoters of the brain-derived neurotrophic factor (Bdnf) gene and thus significantly downregulate BDNF expression and impair the activity of key BDNF signaling pathways (pCaMKII, pErk2, and pCREB). However, treatment with the HDAC inhibitor trichostatin A attenuated all the negative effects induced by SD. Therefore, BDNF and its histone acetylation regulation may play important roles in SD-induced spatial memory impairment, whereas HDAC inhibition possibly confers protection against SD-induced impairment in spatial memory and hippocampal functions.


PubMed | Tianjin Institute of Health and Environmental Medicine, Weifang Peoples Hospital, Tianjin Medical University and Feinstein Institute for Medical Research
Type: | Journal: Neuroscience | Year: 2017

Stress-induced neural injuries are closely linked to the pathogenesis of various neuropsychiatric disorders and psychosomatic diseases. We and others have previously demonstrated certain protective effects of epigallocatechin-3-gallate (EGCG) in stress-induced cerebral impairments, but the underlying protective mechanisms still remain poorly elucidated. Here we provide evidence to support the possible involvement of PKC and ERK1/2 signaling pathways in EGCG-mediated protection against restraint stress-induced neural injuries in rats. In both open-field and step-through behavioral tests, the restraint stress-induced neuronal impairments were significantly ameliorated by administration of EGCG or green tea polyphenols (GTPs), which was associated with a partial restoration of normal plasma glucocorticoid, dopamine and serotonin levels. Furthermore, the stress-induced decrease of PKC and ERK1/2 expression and phosphorylation was significantly attenuated by EGCG and to a less extent by GTP administration. Additionally, EGCG supplementation restored the production of ATP and the expression of a key regulator of cellular energy metabolism, the peroxisome proliferators-activated receptor- coactivator-1 (PGC-1), in stressed animals. In conclusion, PKC and ERK1/2 signaling pathways as well as PGC-1-mediated ATP production might be involved in EGCG-mediated protection against stress-induced neural injuries.

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