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Yu X.-Y.,Nankai Hospital | Zou C.-L.,Tianjin Medical University | Zhou Z.-L.,Nankai Hospital | Shan T.,Nankai Hospital | And 2 more authors.
World Journal of Gastroenterology | Year: 2014

Aim: To investigate the phasic alteration of intestinal homeostasis in an experimental model of intestinal obstruction. Methods: A rabbit model of intestinal obstruction was established by transforming parts of an infusion set into an in vivo pulled-type locking clamp and creating a uniform controllable loop obstruction in the mesenteric non-avascular zone 8 cm from the distal end of the ileum. The phasic alteration of intestinal homeostasis was studied after intestinal obstruction. The changes in goblet cells, intraepithelial lymphocytes, lamina propria lymphocytes, and intestinal epithelium were quantified from periodic acid-Schiff-stained sections. Ornithine decarboxylase (ODC) activity and serum citrulline levels were measured by high-performance liquid chromatography. Claudin 1 mRNA expression was examined by real-time polymerase chain reaction analysis. Intestinal microorganisms, wet/dry weight ratios, pH values, and endotoxin levels were determined at multiple points after intestinal obstruction. Furthermore, the number and ratio of CD3+, CD4+ and CD8+ T cells were determined by flow cytometry, and secretory IgA levels were measured with an enzyme-linked immunosorbent assay. Results: A suitable controllable rabbit model of intestinal obstruction was established. Intestinal obstruction induced goblet cell damage and reduced cell number. Further indicators of epithelial cell damage were observed as reduced serum citrulline levels and claudin 1 gene expression, and a transient increase in ODC activity. In addition, the wet/dry weight ratio and pH of the intestinal lumen were also dramatically altered. The ratio of Bacillus bifidus and enterobacteria was reversed following intestinal obstruction. The number and area of Peyer's patches first increased then sharply decreased after the intestinal obstruction, along with an alteration in the ratio of CD4/CD8+ T cells, driven by an increase in CD3+ and CD8+ T cells and a decrease in CD4+ T cells. The number of lamina propria lymphocytes also gradually decreased with prolonged obstruction. Conclusion: Intestinal obstruction can induce disruption of intestinal homeostasis. © 2014 Baishideng Publishing Group Inc. All rights reserved. Source


Zhang S.-K.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine | Cui N.-Q.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine | Zhuo Y.-Z.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine | Li D.-H.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine | Liu J.-H.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine
Chinese Journal of Integrative Medicine | Year: 2013

Objective: To investigate the effect of modified Xiaochaihu Decoction (MXD) on transforming growth factor-β1/Sma- and Mad-related proteins (TGF-β1/Smads) signaling pathway in rats with chronic pancreatitis (CP) induced by dibutyltin dichloride. Methods: Thirty healthy male Wistar rats were randomly divided into the normal control group, CP group and CP+MXD-treated group. CP was induced by injection of dibutyltin dichloride (DBTC, 7 mg/kg of body weight) into the right caudal vein, and the control rats were treated with vehicle. MXD was given daily by gavage at a dose of 10 g/kg of body weight, starting from the day after CP induction. After 28-day treatment, the n-benzoyl-tyrosyl para-aminobenzoic acid (NBT-PABA) test was carried out to evaluate exocrine pancreatic function. Then, rats were sacrificed, and pancreatic tissues were harvested for histological evaluation. In addition, the mRNA expression of TGF-β1, TGF-β1 type II receptor (TGFβRII), Smad3 and Smad7 was determined in pancreatic tissues by using real-time polymerase chain reaction. Results: Treatment of CP with MXD improved the PABA recovery, decreased the histological lesion, and reduced the mRNA expression of TGF-β1, TGFβRII and Smad3 (P<0.05). However, MXD had no effect on Smad7 mRNA level. Conclusions: MXD could protect the pancreas against chronic injury and improve pancreatic exocrine function in DBTC induced rat CP model. Its mechanism may involve inhibition of the TGF-β1/Smads signaling pathway. © 2013 Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg. Source


Li D.-H.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine | Wang Y.,Tianjin University of Traditional Chinese Medicine | Lv Y.-S.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine | Liu J.-H.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine | And 3 more authors.
BioMed Research International | Year: 2015

The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy. Copyright © 2015 Di-Hua Li et al. Source


Li D.-H.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine | Lv Y.-S.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine | Liu J.-H.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine | Yang L.,Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine | And 3 more authors.
International Journal of Analytical Chemistry | Year: 2016

A HPLC coupled with evaporative light scattering detection method had been developed for the simultaneous determination of 3,4-dihydroxyphenylethyl alcohol glycoside, salidroside, chlorogenic acid, and liriodendrin in the stem of Sargentodoxa cuneata. With a C18 column, the analysis was performed using acetonitrile and 0.2% formic acid aqueous solution as mobile phase in gradient program at a flow rate of 0.9 mL/min. The optimum drift tube temperature of evaporative light scattering detection was at 105°C with the air flow rate of 2.5 L/min. The calibration curves showed good linearity during the test ranges. This method was validated for limits of detection and quantification, precision, and reproducibility. The recoveries were within the range of 96.39%-104.64%. The relative standard deviations of intraday and interday precision were less than 2.90% and 3.30%, respectively. The developed method can be successfully used to quantify the four analytes in the stem of Sargentodoxa cuneata from various regions in China. © 2016 Di-Hua Li et al. Source

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