Wu J.P.,Tianjin Haihe Hospital
Zhonghua yi xue za zhi | Year: 2010
OBJECTIVE: To describe the clinical characteristics of fatal cases with confirmed A H1N1 influenza so as to improve the diagnosis and treatment of this severe disease. METHODS: We retrospectively analyzed the medical records of 11 fatal cases with A H1N1 influenza in Tianjin between September 1 and November 4 2009. RESULTS: There were 6 males and 5 females with a median age of 25 (21 - 36) years old. Two cases were pregnant women and 3 patients suffered such concurrent disorders as dilated cardiomyopathy, multiple myeloma or atrophic gastritis. Over 3 lung fields were involved on chest imaging studies and the oxygenation index was less than 300 mm Hg at admission. The therapies of oseltamivir and methylprednisolone were administered and mechanical ventilation was initiated within 24 hours. Refractory hypoxemia and a higher level of lactate dehydrogenase were present during treatment. Pneumothorax or mediastinal emphysema occurred in 4 patients, acute renal failure in 1 and pneumopyothorax in 1. The culture of airway secretion at 3 - 7 days after mechanical ventilation showed Staphylococcus aureus in 4 patients and Aspergillus in 2. The progress of disease was so quick that the duration from onset of clinical symptoms to hospitalization was a median of 4 (3 - 6) days and the duration from onset of clinical symptoms to death a median of 12 days. CONCLUSION: The fatal cases with A H1N1 influenza in Tianjin occurred mostly in young individuals and pregnant women. This severe disease had a rapid progression. And bacterial co-infections were quite common. Refractory hypoxemia resulting in respiratory failure was the main mortality reason.
Ning G.,Tianjin Medical University |
Tang L.,Tianjin Haihe Hospital |
Wu Q.,Tianjin Medical University |
Li Y.,Tianjin Medical University |
And 2 more authors.
Regenerative Medicine | Year: 2013
Aim: We aim to explore the repair mechanism after the transplantation of CD34+ human umbilical cord blood cells (HUCBCs) in traumatic spinal cord injury (SCI) in rats. Materials & methods: Wistar rats with SCI were randomly divided into three groups: DMEM injection (group A); CD34+ HUCBC transplantation on the first day after injury (group B); and CD34 + HUCBC transplantation on the sixth day after injury (group C). The Basso, Beattie and Bresnahan scores were used to evaluate motor behavior. At the injured site, the infarct size, blood vessel density, and survival and neural differentiation of transplanted cells were analyzed. Results: It was found that the Basso, Beattie and Bresnahan score in group B was significantly higher than other groups (p < 0.05), and the infarct size and blood vessel density at the injured site were significantly different (p < 0.01). However, the transplanted cells survived at least 3 weeks at the injured site, but did not differentiate into neural cells. Conclusion: These results suggested transplantation of CD34+ HUCBCs during the acute phase could promote the functional recovery better than during the subacute phase after SCI by raising blood vessel density, suggesting the possible clinical application for the treatment of spinal injury. © 2013 Future Medicine Ltd.
Mei Z.,Tianjin Haihe Hospital
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi | Year: 2010
The artificial lung is a technical device for providing life support; it will be put in use when the natural lungs are failing and are not able to maintain sufficient oxygenation of the body's organ systems. From the viewpoint of long-term development, the artificial lung should be permanently implanted in the body, so that it will substitute for the human pulmonary function partially or completely. In this paper, four artificial lung technologies were expounded with reference to the development and research process of artificial lung. They were extracorporeal membrane oxygenation, intravascular artificial lung, implantable artificial lung, and pumpless extracorporeal lung assist. In this paper were described the structure of the four kinds of artificial lung, the working principle, and their advantages, disadvantages and indications. The prospect of artificial lung was evaluated in the light of the data from the existing animal experiments and from the clinical experience of the centers.
Fan Y.,Tianjin Haihe Hospital
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases | Year: 2010
To contrast the massive pulmonary embolism of the animal model had been stabled by inserting the animal's own blood clots or through use of detachable latex balloons. Eighteen healthy sheep were separated as three groups with random. We established the animal lung, PE model by inserting the animal's own blood clots at right lung artery in 6 sheep, and 6 sheep were through used of detachable latex balloons to substitute the blood clots. Six healthy sheep were used as controls. Each sheep was anesthetized. Both sides of the region inguinalis were applied 21 transfixion pins to one femoral artery and two femoral veins on the left side, and one femoral vein on the right side. All vessels were inducted into the 5 F vagina vasorum except one vein for which 12 F was inducted. 50 ml blood was taken from vagina vasorum from each animal and stabilized for 2 h for later use as a body blood clot. Guided and visualized by X-ray, a 5 F pigtail catheter was inserted on the left side to measure arterial pressure, central venous pressure and pulmonary arterial pressure; a 12 F guiding catheter was then inserted through right side femoral vein into the right lung artery to inject 8 ml 350 mg I ultravist at 6 ml/s for pulmonary arteriography, After the animal was stabilized for 30 min, we input their own body blood clots (0.6 ml/kg) using a 12 F guiding catheter or, alternatively, to release a self-made, detachable latex balloon. We measured arterial pressure, central venous pressure, pulmonary arterial pressure, heart rate, blood oxygen saturation, partial pressure of oxygen in artery and partial pressure of carbon dioxide in artery etc. of the animals both immediately before inputting their own body blood clots or detachable latex balloons, immediately afterwards, immediately before interfering with airflow and at 3 min, 6 min, 10 min, 15 min, 20 min, 30 min, 45 min and 60 min after the interference. Heart and breath rates of all 12 sheep increased, blood oxygen saturation and partial pressure of oxygen in artery decreased, and pulmonary arterial pressure increased after inputting their own body blood clots or inserting latex balloons with statistical significance that were compared with controls. Blood oxygen saturation decreased, on average, by more than 25% within 30 min. Two hours after, all 12 experimental animals showed stable. Index of sheep in latex balloons group were increased slowly but stabilized at a lower level in blood clots group with statistical significance. This animal experiment demonstrates that balloon can establish a physico- affection and blood clot can establish a chemical action for massive PE.
Liu X.-J.,Tianjin Haihe Hospital |
Zhao M.,Tianjin Medical University
Asian Pacific Journal of Cancer Prevention | Year: 2014
Chemotherapy continues to be a mainstay of cancer treatment, although drug resistance is a major obstacle. Lipid metabolism plays a critical role in cancer pathology, with elevated ether lipid levels. Recently, alkylglyceronephosphate synthase (AGPS), an enzyme that catalyzes the critical step in ether lipid synthesis, was shown to be up-regulated in multiple types of cancer cells and primary tumors. Here, we demonstrated that silencing of AGPS in chemotherapy resistance glioma U87MG/DDP and hepatic carcinoma HepG2/ADM cell lines resulted in reduced cell proliferation, increased drug sensitivity, cell cycle arrest and cell apoptosis through reducing the intracellular concentration of lysophosphatidic acid (LPA), lysophosphatidic acid-ether (LPAe) and prostaglandin E2 (PGE2), resulting in reduction of LPA receptor and EP receptors mediated PI3K/AKT signaling pathways and the expression of several multi-drug resistance genes, like MDR1, MRP1 and ABCG2. β -cateniin, caspase-3/8, Bcl-2 and survivin were also found to be involved. In summary, our studies in dicate that AGPS plays a role in cancer chemotherapy resistance by mediating signaling lipid me tabolism in cancer cells.