Tianjin Fourth Central Hospital

Tianjin, China

Tianjin Fourth Central Hospital

Tianjin, China
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Wang D.,Tianjin Fourth Central Hospital | Zhang J.,Tianjin Fourth Central Hospital
Neural Regeneration Research | Year: 2012

Following successful establishment of a rat model of spinal cord hemisection injury by resecting right spinal cord tissues, bone marrow stem cells were transplanted into the spinal cord lesions via the caudal vein while maintaining rectal temperature at 34 ± 0.5°C for 6 hours (mild hypothermia). Hematoxylin-eosin staining showed that astrocytes gathered around the injury site and formed scars at 4 weeks post-transplantation. Compared with rats transplanted with bone marrow stem cells under normal temperature, rats transplanted with bone marrow stem cells under hypothermia showed increased numbers of proliferating cells (bromodeoxyuridine-positive cells), better recovery of somatosensory-evoked and motor-evoked potentials, greater Basso, Beattie, and Bresnahan locomotor rating scores, and an increased degree of angle in the incline plate test. These findings suggested that hypothermia combined with bone marrow mesenchymal stem cells transplantation effectively promoted electrical conduction and nerve functional repair in a rat model of spinal cord hemisection injury.


Ye K.,Tianjin Fourth Central Hospital
Chinese Journal of Tissue Engineering Research | Year: 2016

BACKGROUND: Studies have shown that human amniotic mesenchymal stem cells can differentiate into hepatocyte-like cells, suggesting that human amniotic mesenchymal stem cell transplantation provides a new potential for the clinical treatment of liver diseases. OBJECTIVE: To observe the effect of human amniotic mesenchymal stem cell transplantation on the repair of liver ischemia-reperfusion injury repair. METHODS: Sixty Sprague-Dawley rats were randomized into stem cell transplantation, model and control groups. Animal models of liver ischemia-reperfusion injury were made in the rats in the stem cell transplantation and model groups. One hour after modeling, rats in the stem cell transplantation were given injection of human amniotic mesenchymal stem cells (0.5 mL, 106 cells) via the tail vein, while rats in the model and control group were given L-DMEM (0.5 mL) or normal saline (0.5 mL), respectively. Liver function and liver morphology were detected at 1, 2, 3 weeks after transplantation. Meanwhile, RT-PCR detection and western blot assay were also conducted. RESULTS AND CONCLUSION: (1) Liver function: Compared with the control group, levels of aspartate aminotransferase, alanine aminotransferase and malondialdehyde were significantly increased in the model group at different time points after transplantation (P < 0.05), while a significant reduction in the levels of these three indicators was found after cell transplantation as compared with the model group (P < 0.05). (2) Liver morphology: 2 weeks after transplantation, rats in the model group exhibited hepatocyte degeneration and necrosis, and severe fibrosis, but these changes were remarkably alleviated in the stem cell transplantation group. (3) PT-PCR and western blot detection: 2 weeks after transplantation, a significantly higher level of hepatocyte growth factor in the liver tissue and a lower level of α-smooth muscle protein were found in the stem cell transplantation group compared with the model group (P < 0.05). All these experimental findings indicate that human amniotic mesenchymal stem cell transplantation can improve impaired liver function in rats, possibly through regulating hepatocyte growth factor and α-smooth muscle protein expression levels in the liver, and thereby promotes the repair of liver ischemia-reperfusion injury. © 2016, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Liu X.-H.,Tianjin Fourth Central Hospital
Chinese Journal of Tissue Engineering Research | Year: 2016

BACKGROUND: Stem cell transplantation is a promising treatment for type 1 diabetes mellitus. Adipose-derived mesenchymal stem cells have become another hotspot following bone marrow mesenchymal stem cells. OBJECTIVE: To investigate the therapeutic effect of adipose-derived mesenchymal stem cell transplantation on type 1 diabetes in a rat model. METHODS: Forty-five Sprague-Dawley rats were randomized into normal, model and cell transplantation. Animal model of type 1 diabetes was made in the latter two groups through intraperitoneal injection of streptozotocin. Seven days after modeling, rats in the three groups were given intraperitoneal injection of normal saline, serum-free DMEM or adipose-derived mesenchymal stem cell suspension, respectively. Two weeks after injection, body mass, blood glucose level, insulin secretion and PDX-1 mRNA in the pancreatic tissue of rats were monitored and detected in the three groups. RESULTS AND CONCLUSION: After modeling, the body mass of rats were lowered, and increased gradually in the cell transplantation group at 2 weeks after cell transplantation, but it was still decreased in the model group. Compared with the normal group, the fasting blood glucose level was significantly higher in the model group (P < 0.05), but it was reduced significantly after cell transplantation (P < 0.05). Compared with the normal group, the insulin level was reduced significantly in the model group (P < 0.05), but it was increased significantly after cell transplantation (P < 0.05). Highest and lowest PDX-1 mRNA expressions were obtained in the normal and model groups, respectively; and there was a significant difference between groups (P < 0.05). All these findings show that adipose-derived mesenchymal stem cell transplantation relieves hyperglycemia in rats by promoting the expression of PDX-1 in the rat pancreatic tissue. © 2016, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Wang D.,Tianjin Fourth Central Hospital | Fan Y.H.,Hebei North University | Zhang J.J.,Tianjin Fourth Central Hospital
Neural Regeneration Research | Year: 2013

Inhibition of neurite growth, which is in large part mediated by the Nogo-66 receptor, affects neural regeneration following bone marrow mesenchymal stem cell transplantation. The tissue engineering scaffold poly(D,L-lactide-co-glycolic acid) has good histocompatibility and can promote the growth of regenerating nerve fibers. The present study used small interfering RNA to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells and Schwann cells, which were subsequently transplanted with poly(D,L-lactide-co-glycolic acid) into the spinal cord lesion regions in rats. Simultaneously, rats treated with scaffold only were taken as the control group. Hematoxylin-eosin staining and immunohistochemistry revealed that at 4 weeks after transplantation, rats had good motor function of the hind limb after treatment with Nogo-66 receptor gene-silenced cells plus the poly(D,L-lactide-co-glycolic acid) scaffold compared with rats treated with scaffold only, and the number of bone marrow mesenchymal stem cells and neuron-like cells was also increased. At 8 weeks after transplantation, horseradish peroxidase tracing and transmission electron microscopy showed a large number of unmyelinated and myelinated nerve fibers, as well as intact regenerating axonal myelin sheath following spinal cord hemisection injury. These experimental findings indicate that transplantation of Nogo-66 receptor gene-silenced bone marrow mesenchymal stem cells and Schwann cells plus a poly(D,L-lactide-co-glycolic acid) scaffold can significantly enhance axonal regeneration of spinal cord neurons and improve motor function of the extremities in rats following spinal cord injury.


Wang Z.-Q.,The 150th Hospital of Chinese PLA | Zhang J.-J.,Tianjin Fourth Central Hospital
Chinese Journal of Tissue Engineering Research | Year: 2016

BACKGROUND: Human leukocyte antigen F-associated transcription factor 10 (FAT10) is highly expressed in many tumor cells like colon cancer cells, but its relationship with esophageal cancer is less reported. OBJECTIVE: To investigate the effects of siRNA interference technique on the invasion, apoptosis and the characteristics of EC9706 cells, a human esophageal cancer cell line. METHODS: siRNA sequence was designed and synthesized according to the FAT10 mRNA encoding sequence, and the EC9706 cells were transiently transfected. EC9706 cells were divided into three groups: siRNA FAT10 group, negative control group, and blank control group. The expression levels of bcl-2 and FAT10 were detected by RT-PCR and western blot assay, respectively. Cell counting kit-8 assay was used to measure the proliferation of cells in vitro. Flow cytometry was used to observe the changes of cell cycle, cell apoptosis and the expression of CD44+ CD133+. TUNEL staining was used to detect the apoptosis of the cells. Cell invasion in vitro was detected by Transwell invasion assay. RESULTS AND CONCLUSION: RT-PCR and western blot findings showed that compared with the negative control group and blank control group, the expression levels of bcl-2 and FAT10 mRNA and protein were significantly decreased in the siRNA FAT10 group (P < 0. 05); the percentage of CD44+ CD133+ cells was decreased significantly (P < 0. 05); and significantly increased apoptosis rate, and decreased cell proliferation and invasion were also found in the siRNA FAT10 group (P < 0. 05). In conclusion, the specific silencing of FAT10 gene can reduce the invasion of esophageal cancer cells, inhibit cell proliferation, reduce bcl-2 expression, and increase the apoptosis rate. Meanwhile, the proportion of CD44+ CD133+ cells is decreased. © 2016, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Wei Q.,Tianjin Fourth Central Hospital | Yang P.,Jilin University
Journal of Jilin University Medicine Edition | Year: 2017

Objective: To investigate the effects of telmisartan on the expressions of activin A and its receptors in non-infarction area of left ventricle in the heart failure rats after myocardial infarction, and to clarify the mechanism of improvement effect of telmisartan on myocardial collagen deposition. Methods: A total of 27 rats were divided into sham operation group (n = 9), model group (h=20) and telmistartan group (n=10). The left anterior descending coronary arteries of the rats in model group and telmisartan group were ligated, and the left anterior descending coronary arteries of the rats in sham operation group were not ligated. After 5 weeks, there were 6 surviving rats in each group. The rats in sham operation group were treated with 0. 5%CMC (10 mL • kg-1), the rats in model group were treated with 0.5% CMC (10 mL • kg-1), and the rats in telmisartan group were treated with telmisartan (30 mg •kg-1 ). AH the rats were treated with medicines by intragastric administration for 4 weeks. The whole cardiac hypertrophy index and left ventricular hypertrophy index of the rats were measured; the mRNA expression levels of activin A, ActRII A, ActR 0 B, Col I , and Col ID in the non-infarction area of the rats were detected by RT-PCR and the ratio of Col I / ColQI was calculated; the expression intensities of activin A protein in the myocardium tissue in non-infarction area of the rats in various groups were observed by immunohistochemical staining. Results: Compared with sham operation group, the whole cardiac hypertrophy index and left ventricular hypertrophy index of the rats in model group were significantly increased (P<0. 01); the mRNA expression levels of activin A, ActRII A and ActRII B, Col I , Colffl and the ratio of Col I /Coll were also increased (P<0. 01). The immunohistochemical staining results showed that the expression level of activin A protein in noninfarction area of left ventricle of the rats in sham operation group was lower, and the expression level of activin A protein in non- infarction area of left ventricle of the rats in model group was higher. Conclusions Telmisartan may improve the myocardial collagen deposition by inhibiting the expressions of activin A and its receptors during heart failure.


PubMed | Tianjin Nankai Hospital and Tianjin Fourth Central Hospital
Type: Journal Article | Journal: Cell biology and toxicology | Year: 2016

In the present study, we determined the protective role of lutein against A


Wang D.,Tianjin Fourth Central Hospital
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2010

Bone marrow mesenchymal stem cells (BMSCs) play an important role in repairing nerve injury, meanwhile external temperature has significant effect on BMSCs transplantation, proliferation, and differentiation. To investigate the effect of BMSCs transplantation and mild hypothermia on repair of rat spinal cord injury (SCI). Forty-five female adult SD rats (weighing 200-250 g) were made the models of hemitransection SCI and divided randomly into 3 groups according to different treatments: group A (SCI group), group B (BMSCs transplantation group), and group C [BMSCs transplantation combined with mild hypothermia (33-35 degrees C) group]. At 1, 2, 4, 6, and 8 weeks after injury, the function of hind limb was evaluated with Basso Beattie and Bresnahan (BBB) score and inclined plane test. At 4 weeks after injury, histopathology and BrdU immunohistochemistry staining were performed. At 8 weeks after injury, horseradish peroxidase (HRP) retrograde nerve trace and transmission electron microscope (TEM) testing were performed to observe the regeneration of axon. After 4 weeks, the function of hind limb obviously recovered in groups B and C, there were significant differences in BBB score between groups B, C and group A (P < 0.05), between group B and group C (P < 0.05). There was no significant difference (P > 0.05) in tilt angle among 3 groups after 1 and 2 weeks, and there were significant differences (P < 0.05) among 3 groups after 4 weeks. HE staining showed that significant cavity could be seen in group A, little in group B, and no cavity in group C. BrdU immunohistochemistry staining showed that the number of positive cells was 0, 90.54 +/- 6.23, and 121.22 +/- 7.54 in groups A, B, and C, respectively; showing significant differences (P < 0.01) among 3 groups. HRP retrograde neural tracing observation showed that the number of HRP positive nerve fibers was 10.35 +/- 1.72, 43.25 +/- 2.65, and 84.37 +/- 4.59 in groups A, B, and C, respectively, showing significant differences (P < 0.01) among 3 groups. TEM observation showed that a great amount of unmyelinated nerve fibers and myelinated nerve fibers were found in central transverse plane in group C. The BMSCs transplantation play an important role in promotion of recovering the function of hind limb after SCI, and mild hypothermia has synergism effects.


Luo Z.K.,Tianjin Fourth Central Hospital
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2012

To observe the clinical efficacy and safety of Puerarin Injection treatment on angina pectoris. 388 patients with angina pectoris, enrolled to Tianjin Fourth Central Hospital during January 2009 and February 2011 were selected and randomly divided into treatment or control groups with 194 patients of each. Based on the conventional therapy program, one group was given Puerarin Injection as treatment group while, the other was given with Danshen Injection as control group. Clinical efficacy, the attack rate of angina pectoris, oxygen consumption, indices on electrocardiogram, haemorheology and other adverse reactions among the two groups were compared. The total efficacy of the treatment group (88.14%, 171/194) was significantly higher than the control group (61.86%, 120/194) and the difference was statistically significant (P < 0.05). During the treatment, no significant adverse events were noticed in both of the two groups of patients. The Puerarin Injection treatment program on angina pectoris seemed effective and safe.


PubMed | Tianjin Fourth Central Hospital
Type: Journal Article | Journal: Saudi medical journal | Year: 2017

To investigate whether there is a difference in carbohydrate antigen 19-9 (CA19-9), carcinoembryonic antigen (CEA), carbohydrate antigen 72-4 (CA72-4), and neuron-specific enolase (NSE) between diabetic and non-diabetic patients.Methods: A retrospective analysis was performed in 268 type 2 diabetic patients and 95 non-diabetic ones, and their serum levels of CA19-9, CEA, CA72-4, and NSE were compared in our endocrine ward at the Tianjin Fourth Central Hospital, Tianjin, Chinaduring the period from January to June 2015. The diabetic patients were divided into 4 groups based on glycosylated hemoglobin (HbA1c) levels to investigate the relationship between levels of tumor markers and glucose status.Results: Diabetic patients had higher levels of tumor markers than non-diabetic subjects (CA19-9: 13.0 versus 7.25U/mL, p=0.000; CEA: 2.55 versus 2.25 ng/mL, p=0.012; CA72-4: 1.95 versus 1.50U/mL, p=0.001; NSE: 11.64 versus 10.22ng/mL, p=0.000). CA19-9 levels increased in a stepwise manner with poor diabetes status. CEA levels were increased in patients with HbA1c 9% and CA72-4 elevation was predominant in patients with poor glycemic control (HbA1c 11%). NSE levels were not associated with metabolic parameters.Conclusion: Serum levels of CA19-9, CEA, CA72-4, and NSE were elevated in type 2 diabetes; however, only CA19-9, CEA, and CA72-4 levels were associated with hyperglycemia.

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