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Huang H.,Tianjin Medical University | Huang H.,Tianjin Fourth Center Hospital | Liang H.,Tianjin Medical University | Zhan Z.-L.,Tianjin Medical University | And 3 more authors.
Medical Oncology

The stomach is the most common site of gastrointestinal stromal tumors (GISTs), but the surgical outcomes of gastric GISTs in the era of targeted drug therapy are unclear. This study aimed to assess factors associated with adverse outcomes and to analyze the effects of targeted drug therapy on gastric GISTs. The surgical outcomes and follow-up records of consecutive patients with gastric GISTs treated at Tianjin Medical University Cancer Institute & Hospital between June 2002 and December 2008 were reviewed. Eighty-five patients were included. Surgery was undertaken in all patients with curative intent. Imatinib mesylate was administered preoperatively to 6 (7%) patients (neoadjuvant therapy), and the median durations of therapy were 6 months (range 3-17 months). Imatinib mesylate was administered postoperatively to 18 (21%) patients with high-risk lesions (adjuvant therapy) and 19 (22%) patients with recurrent disease, and the median durations of therapy were 22 months (range 6-24 months) and 25 months (range 1-64 months), respectively. Tumor size greater than 10 cm (P = 0.015), high mitotic index (P = 0.021), and no adjuvant imatinib therapy (P = 0.046) were the only significant factors associated with higher recurrence-free survival in multivariate analysis. Large tumors, high mitotic index, and the absence of imatinib treatment are associated with high recurrencefree survival. Adjuvant imatinib therapy of 2 years appears to decrease the recurrence of gastric GISTs. © Springer Science+Business Media, LLC 2011. Source

Qu X.,Tianjin Fourth Center Hospital | Di X.,Tianjin Fourth Center Hospital | Han L.,Tianjin Fourth Center Hospital | Zhang H.-C.,Tianjin Fourth Center Hospital
Chinese Journal of Tissue Engineering Research

Background: How to obtain a sufficient number of cells is one of the key issues in the cell transplantation therapy, and studies have shown that stem cell proliferation can be promoted by reasonable stimulus. Objective:To investigate reduced glutathione effects on biological characteristics of human umbilical cord blood mesenchymal stem cells. Methods: The cells were divided into two groups: the control group consisted of the normal human umbilical cord blood mesenchymal stem cells, and in the experimental group, human umbilical cord blood mesenchymal stem cells were treated with 0.15 g/L reduced glutathione. Results and Conclusion: At days 5, 7, 9, cells treated with 0.15 g/L reduced glutathione showed higher absorbance values than those in the control group (P < 0.05). Flow cytometry showed reduced glutathione had no effects on CD29, CD44, CD45, CD105 expression. Real-time PCR results showed reduced glutathione was capable of promoting extracellular signal-regulated kinase mRNA expression (P < 0.05). Findings from this study showed that 0.15 g/L reduced glutathione can promote the proliferation of humanumbilical cord blood mesenchymal stem cells. Source

Lei X.,Tianjin Fourth Center Hospital | Lei L.,Shanxi Medical University | Zhang Z.,Tianjin Fourth Center Hospital | Cheng Y.,Tianjin Fourth Center Hospital
Molecular Medicine Reports

The present study aimed to investigate the neuroprotective effect of lycopene in a mouse model of bilateral common carotid artery occlusion (BCCAO) and the role of the Nrf2/HO-1 signaling pathway. A total of 60 male C57BL/6 mice, aged 12 weeks and weighing 20-24 g, were used in the present study. The mice were randomly assigned to three groups: Control, BCCAO and BCCAO + lycopene. The neurological score was assessed 24, 48 or 72 h following BCCAO. Hematoxylin and eosin staining, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) were performed to detect neuronal death and survival. The production of glutathione (GSH) and reactive oxygen species were detected to investigate the oxidative stress. The expression levels of nuclear factor erythroid 2-related factor (Nrf2) and Heme oxygenase-1 (HO-1) were determined by western blotting. Lycopene significantly improved the neurological score in the BCCAO mice. It attenuated neuronal apoptosis, as indicated by TUNEL staining, and attenuated the oxidative stress induced by global ischemia. Lycopene increased the expression levels of Nrf2 and HO-1, indicating that the Nrf2/HO-1 signaling pathway may be involved in the neuroprotective effect of lycopene. The present study revealed that lycopene protects the brain from global ischemic injury, which is associated with its antiapoptotic effect and the activation of the Nrf2/HO-1 signaling pathway. Source

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