Tianjin Fourth Center Hospital

Tianjin, China

Tianjin Fourth Center Hospital

Tianjin, China

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Lei X.,Tianjin Fourth Center Hospital | Lei L.,Shanxi Medical University | Zhang Z.,Tianjin Fourth Center Hospital | Cheng Y.,Tianjin Fourth Center Hospital
Molecular Medicine Reports | Year: 2016

The present study aimed to investigate the neuroprotective effect of lycopene in a mouse model of bilateral common carotid artery occlusion (BCCAO) and the role of the Nrf2/HO-1 signaling pathway. A total of 60 male C57BL/6 mice, aged 12 weeks and weighing 20-24 g, were used in the present study. The mice were randomly assigned to three groups: Control, BCCAO and BCCAO + lycopene. The neurological score was assessed 24, 48 or 72 h following BCCAO. Hematoxylin and eosin staining, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) were performed to detect neuronal death and survival. The production of glutathione (GSH) and reactive oxygen species were detected to investigate the oxidative stress. The expression levels of nuclear factor erythroid 2-related factor (Nrf2) and Heme oxygenase-1 (HO-1) were determined by western blotting. Lycopene significantly improved the neurological score in the BCCAO mice. It attenuated neuronal apoptosis, as indicated by TUNEL staining, and attenuated the oxidative stress induced by global ischemia. Lycopene increased the expression levels of Nrf2 and HO-1, indicating that the Nrf2/HO-1 signaling pathway may be involved in the neuroprotective effect of lycopene. The present study revealed that lycopene protects the brain from global ischemic injury, which is associated with its antiapoptotic effect and the activation of the Nrf2/HO-1 signaling pathway.


PubMed | Tianjin Fourth Center Hospital and Shanxi Medical University
Type: Journal Article | Journal: Molecular medicine reports | Year: 2015

The present study aimed to investigate the neuroprotective effect of lycopene in a mouse model of bilateral common carotid artery occlusion (BCCAO) and the role of the Nrf2/HO1 signaling pathway. A total of 60 male C57BL/6 mice, aged 12 weeks and weighing 2024 g, were used in the present study. The mice were randomly assigned to three groups: Control, BCCAO and BCCAO + lycopene. The neurological score was assessed 24, 48 or 72 h following BCCAO. Hematoxylin and eosin staining, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) were performed to detect neuronal death and survival. The production of glutathione (GSH) and reactive oxygen species were detected to investigate the oxidative stress. The expression levels of nuclear factor erythroid 2related factor (Nrf2) and Heme oxygenase1 (HO1) were determined by western blotting. Lycopene significantly improved the neurological score in the BCCAO mice. It attenuated neuronal apoptosis, as indicated by TUNEL staining, and attenuated the oxidative stress induced by global ischemia. Lycopene increased the expression levels of Nrf2 and HO1, indicating that the Nrf2/HO1 signaling pathway may be involved in the neuroprotective effect of lycopene. The present study revealed that lycopene protects the brain from global ischemic injury, which is associated with its antiapoptotic effect and the activation of the Nrf2/HO1 signaling pathway.


Qu X.,Tianjin Fourth Center Hospital | Di X.,Tianjin Fourth Center Hospital | Han L.,Tianjin Fourth Center Hospital | Zhang H.-C.,Tianjin Fourth Center Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: How to obtain a sufficient number of cells is one of the key issues in the cell transplantation therapy, and studies have shown that stem cell proliferation can be promoted by reasonable stimulus. Objective:To investigate reduced glutathione effects on biological characteristics of human umbilical cord blood mesenchymal stem cells. Methods: The cells were divided into two groups: the control group consisted of the normal human umbilical cord blood mesenchymal stem cells, and in the experimental group, human umbilical cord blood mesenchymal stem cells were treated with 0.15 g/L reduced glutathione. Results and Conclusion: At days 5, 7, 9, cells treated with 0.15 g/L reduced glutathione showed higher absorbance values than those in the control group (P < 0.05). Flow cytometry showed reduced glutathione had no effects on CD29, CD44, CD45, CD105 expression. Real-time PCR results showed reduced glutathione was capable of promoting extracellular signal-regulated kinase mRNA expression (P < 0.05). Findings from this study showed that 0.15 g/L reduced glutathione can promote the proliferation of humanumbilical cord blood mesenchymal stem cells.


Chen X.,Tianjin Medical University | Chen X.,Tianjin Fourth Center Hospital | Cao J.,Tianjin Medical University | Cao J.,Tianjin Fourth Center Hospital | And 2 more authors.
National Medical Journal of China | Year: 2016

Objective: To observe the interleukin-17 (IL-17) expression in lung tussiue of patients with stable chronic obstructive pulmonary disease ( COPD) and investigate the clinical significance. Methods: A total of 50 patients receiving lung resections in General Hospital, Tianjin Medical University for lung cancer from October 2010 to October 2011 were included and divided into non-smoking COPD group (NS group) , smoking non-COPD group ( S group) , smoking COPD group ( COPD group) according to smoking status and 2013 COPD diagnosis standard. The expression of IL-17 was detected by immumohistochemical staining. The volume of IL-17 in the airway and lung parenchyma was calculated by immumohistochemical staining scores which was product of dyeing cells positive rate and intensity. Pearson correlation analysis was used to analyze the relationships between expression of IL-17 in the airway and lung parenchyma and forced expiratory volume in 1 second/predicted value ( FEV1 % pred) , carbon monoxide diffusion amount accounting for the percentage of the expected value ( DLCO% pred) , COPD assessment test (CAT) score, body mass index (BMI). Results: The expressions of IL-17 in airway and lung parenchyma in S group (5.6 ±3.1 , 6.4 ±2.5) were higher than those in NS group (1.6 ±1.4, l.4±l.2) (both P < 0.05 ) , while expressions in COPD group (7.3±2.5,7.8±2.1) were further higher than S group ( both P < 0.05). In the S group and COPD group, the expressions of IL-17 in the airway and lung parenchyma were positively correlated with smoking ( r = 0.453 , P = 0.007 and r = 0.573 , P < 0.001 ) ; In the COPD group, the expressions of IL-17 in the airway and lung parenchyma were negatively correlated with FEV1% pred (r = - 0.729, P = 0.001 and r = - 0.489, P = 0.039) , negatively related with DLCO% pred ( r = -0.493, P = 0.038 and r = -0.520, P= 0.027), positively correlated with COPD CAT score (r = 0.730, P = 0.001 and r = 0.644, P = 0.004 ) and negatively related with BMI (r = - 0.653 , P = 0.003 and r = -0.485, P =0.041). Conclusion: Smoking increases inflammation of lung tissue, increases IL-17 expression in airway and lung parenchyma; the expression of IL-17 is closely related to the severity of disease, the quality of life and prognosis in COPD patients. Copyright © 2016 by the Chinese Medical Association.


Huang H.,Tianjin Medical University | Huang H.,Tianjin Fourth Center Hospital | Liang H.,Tianjin Medical University | Zhan Z.-L.,Tianjin Medical University | And 3 more authors.
Medical Oncology | Year: 2012

The stomach is the most common site of gastrointestinal stromal tumors (GISTs), but the surgical outcomes of gastric GISTs in the era of targeted drug therapy are unclear. This study aimed to assess factors associated with adverse outcomes and to analyze the effects of targeted drug therapy on gastric GISTs. The surgical outcomes and follow-up records of consecutive patients with gastric GISTs treated at Tianjin Medical University Cancer Institute & Hospital between June 2002 and December 2008 were reviewed. Eighty-five patients were included. Surgery was undertaken in all patients with curative intent. Imatinib mesylate was administered preoperatively to 6 (7%) patients (neoadjuvant therapy), and the median durations of therapy were 6 months (range 3-17 months). Imatinib mesylate was administered postoperatively to 18 (21%) patients with high-risk lesions (adjuvant therapy) and 19 (22%) patients with recurrent disease, and the median durations of therapy were 22 months (range 6-24 months) and 25 months (range 1-64 months), respectively. Tumor size greater than 10 cm (P = 0.015), high mitotic index (P = 0.021), and no adjuvant imatinib therapy (P = 0.046) were the only significant factors associated with higher recurrence-free survival in multivariate analysis. Large tumors, high mitotic index, and the absence of imatinib treatment are associated with high recurrencefree survival. Adjuvant imatinib therapy of 2 years appears to decrease the recurrence of gastric GISTs. © Springer Science+Business Media, LLC 2011.


PubMed | Tianjin Fourth Center Hospital
Type: Comparative Study | Journal: Orthopaedic surgery | Year: 2011

A femoral compound interlocking intramedullary nail (FCIIN) was designed to treat all types of fractures between the trochanter and epicondyle of both femurs. It could substitute for femoral interlocking intramedullary nails (FIIN) at five points.According to the morphological characteristics of the femoral medullary canal, the nail is designed to accommodate a 1250 mm radius of radian and a 135 neck-shaft angle. Three interlocking holes of 6.5 mm diameter are located at the proximal end of the FCIIN, making crossing of the screws possible. The hole is designed to be vertical (90) or oblique (45 upper or lower). At the tip of the proximal interlocking screws, whose root diameter gradually increases from 3.5 mm to 6.5 mm, a self-tapping cancellous screw is placed. There are two types of distal interlocking screws. One is a fine thread and the other a bolt screw. Two interlocking holes and a recess 4.5 mm in diameter are located at the distal end of the FCIIN. Under biomechanical destructive testing, the proximal interlocking screw device has satisfactory strength and reasonable structure. A total of 47 patients (31 males and 16 females, with an average age of 39.83 years) with femoral fractures were assessed in this study. Fourteen cases were diagnosed as intertrochanteric, 7 as subtrochanteric, 18 as femoral shaft, and 8 as supracondylar fractures. All 47 patients were treated with the FCIIN.Of the 47 patients, anatomic reduction was achieved in 34, good reduction in 11, and forced line reduction in 2 cases. Reduction was excellent or good in 95.87% of the fractures. The removal time of the FCIIN was 12 to 21 months (average, 16.9 months). One patient with an intertrochanteric fracture who had a fixation failure combined with non-union achieved healing with an external fixator at 18 months. Failure to insert the distal interlocking screws occurred in 5 patients but did not affect bone healing.The FCIIN is a useful device in the treatment of a variety of femoral fractures.

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