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Zhang X.,Huazhong Agricultural University | Wang W.,Huazhong Agricultural University | Huang S.,Huazhong Agricultural University | Chen G.,Huazhong Agricultural University | And 3 more authors.
Mitochondrial DNA | Year: 2014

Dojo loach Misgurnus anguillicaudatus, a small-sized freshwater fish species, is not only one of the most important cultured fish in East Asia, but also a promising model animal to study evolutionary biology for polyploidy. We sequenced the complete mitochondrial genomes from the natural diploid and tetraploid individuals of this species. The two different cytotypes loaches share the same length (16,646 bp) and structural organization of mitochondrial genome. The two genomes have 98.3% nucleotide sequence similarity, and the biggest nucleotide sequence divergence between homologous genes is observed in CYTB gene. © 2014 Informa UK Ltd.

Luo Z.,Huazhong Agricultural University | Luo Z.,Tianjin Fishery Research Institute | Bai X.H.,Tianjin Fishery Research Institute | Chen C.F.,Huazhong Agricultural University
Fisheries Science | Year: 2014

In this study, 342 bacterial strains were isolated from the gut of channel catfish Ictalurus punctatus. Two different screening strategies, including the production of antagonistic effects against pathogens and the capacity of secreting exoenzyme, were used to select potential probiotics from the bacterial strains. Among them, 6 isolates showed antagonism against the pathogens, 15 isolates could produce high amylase and protease, and 3 isolates (BHI344, BH0142 and BH03124) had both the two characteristics. Then, BHI344, BH0142, and BH03124 were tested for survival rate in channel catfish bile and at low pH, antimicrobial susceptibilities, and pathogenicity in channel catfish. The results of the obtained assays showed that BHI344 possessed the broadest inhibitory potential, a strong ability of secreting extra-cellular amylase and protease, high viability in simulated gastrointestinal conditions, and sensitivity to most of the used antibiotics and non-pathogenic to channel catfish. Therefore, it could be selected as the most promising probiotics candidate. BHI344 was identified as Bacillus subtilis based on the phenotypic characterization and 16S rRNA gene sequencing. © 2014, Japanese Society of Fisheries Science.

Luo Z.,Tianjin Fishery Research Institute | Bai X.,Tianjin Fishery Research Institute | Yunxia X.U.,Center for Control and Prevention of Aquatic Animal Infection Disease | Zhang Z.,Tianjin Fishery Research Institute | And 3 more authors.
Israeli Journal of Aquaculture - Bamidgeh | Year: 2015

An epizootic was discovered in grass carp (Ctenopharyngodon idellus) in earthen ponds in Ninghe, Tianjin Province, northern China, from May to June 2013. The cumulative mortality was 30% within 25 days, and the diseased fish presented with hemorrhaging in the abdominal wall, ascites in the abdomen and pale liver. Strains named PA131207 and PA131208 were isolated from the moribund fish, and were identified as Pseudomonas aeruginosa according to both biochemical tests and phylogenetic analysis derived from the 16S rRNA gene. The pathogenicity of PA131207 was confirmed by infectivity experiments and histopathological examination. Antimicrobial susceptibility tests showed that PA131207 was resistant to 9 of 15 antimicrobial agents tested. © 2015 Israeli Journal of Aquaculture - Bamidgeh. All rights reserved.

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