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Zhang B.Y.,Tianjin Nankai Hospital | Zhao Z.,Tianjin Entry Exit Inspection and Quarantine Bureau | Jin Z.,Tianjin Nankai Hospital
Genetics and Molecular Research | Year: 2016

Myocarditis is a common cardiovascular disease and frequently occurs in children and teenagers. It is believed to be caused by both endogenous and exogenous factors, among which FAS/FASL gene pair-induced cell apoptosis is a major mechanism of myocardial cell injury. A previous study has detected low expression of microRNA (miR)-98 in myocarditis patients. Therefore, in this study we investigated the functional implications of miR-98 with respect to the disease. We carried out a case-control study including 50 myocarditis patients and 50 healthy individuals. Total RNA was extracted from peripheral blood plasma. Expression levels of miR-98 and the FAS/FASL gene pair were determined by real-time fluorescent quantitative polymerase chain reaction. The interaction between miR-98 and the FAS/FASL pair was visualized by dual-luciferase reporter assay. The expression of the FAS/FASL gene pair was further detected by transfecting with an miR-98 mimic or an miR-98 inhibitor. The content of miR-98 in the peripheral blood of the myocarditis patients was significantly lower than in the healthy individuals. However, the FAS/FASL genes were upregulated by 1.68-fold in the myocarditis patients. miR-98 was shown to interact with the 3′-untranslated region of the FAS/FASL gene pair. The inhibition/facilitation of miR-98 expression in myocardial cells can modulate apoptosis. miR-98 was downregulated in the peripheral blood of myocarditis patients. It may interact with the FAS/FASL gene pair to further modulate cell apoptosis. © FUNPEC-RP.

Wang G.-X.,Hunan Institute of Science and Technology | Lu M.,Jingdezhen Ceramic Institute | Li J.,Tianjin Entry Exit Inspection and Quarantine Bureau | Liu L.-C.,Hunan Institute of Science and Technology | And 3 more authors.
Iranian Polymer Journal (English Edition) | Year: 2013

Random copolymers of P(MMA-co-styrene) were synthesized via single electron transfer-living radical polymerization (SET LRP) at 25 C in N,N-dimethylformamide (DMF) and benzene using CCl4 as initiator and Fe(0) wire/N,N,N′,N′-tetramethyl-1,2-ethanediamine (TMEDA)/hydrazine (NH2NH2) complexes as catalyst in the presence of air. Fe(0) wire-mediated single electron transfer-living radical copolymerization of MMA and styrene represented a robust and versatile technique to synthesize the well-defined copolymers. The copolymerization rate was faster in DMF than in benzene, as determined by the apparent rate constants. The results showed that the copolymerization followed first-order kinetics model in the presence of polar DMF and non-polar benzene. The molecular weights increased linearly with the increase of monomer conversion with a narrow polydispersity index when the conversion was beyond 25 %. The polarity and the quantity of solvent had significant effects on the polymerization, and the apparent rate constants were 1.28 × 10-4, 1.21 × 10-4, and 9.23 × 10-5 s-1 in the order of DMF amount, 5, 10, and 15 mL. The conversion increased from 29.3 to 48.5 % and the polydispersity index (PDI) changed from 1.24 to 1.21 with [CCl4]0/[TMEDA]0 molar ratio changing from 1:0.5 to 1:5. The chain extension experiment demonstrated that the copolymerization exhibited a living characteristic. © 2012 The Author(s).

Cui J.,Tianjin University | Xiao Y.-B.,Tianjin Entry Exit Inspection and Quarantine Bureau | Dai L.,Tianjin University | Zhao X.-H.,Tianjin University | Wang Y.,Tianjin University
Food Analytical Methods | Year: 2013

Concerning the residual organoarsenical feed additives, an effective method has been developed for the separation and determination of organoarsenic species including p-arsanilic acid (ASA), nitarsone (NIT) and roxarsone (ROX) in the food of animal tissue origin by high-performance liquid chromatography coupled to ultraviolet oxidation hydride generation atomic fluorescence spectrometry using a C18 column with 50 mM KH2PO4, 0.1 % v/v trifluoroacetic acid at pH 2.43 as the mobile phase. Accelerated solvent extraction (ASE) as an effective sample preparation method was used to deal with animal meat to extract organoarsenic species. The ASE conditions, including extraction solvent, temperature, static extraction time, flush volume and cycle time, were investigated in terms of extraction yield and species stability. In this paper, aimed to separate these species efficiently, the conditions of the mobile phase and HG system were also investigated. The methodology developed allows us limits of detection and quantification of 0.24, 0.74, 0.41 and 0.72, 2.24, 1.24 ng mL-1 for ASA, NIT and ROX, respectively. This method was used to separate and determine three organoarsenic species in porcine and chicken liver samples that were purchased at a supermarket in China. At the optimized conditions, the ranges of concentrations of the three arsenic species were found to be varied from 3 to 9 ng mL-1. The results of recovery rates and RSDs, which were higher than 94 % and lower than 5 %, respectively, approved it to be a convenient, fast and efficient method for the determination of organoarsenic species in animal tissue. © 2012 Springer Science+Business Media New York.

Wang S.,Tianjin University of Science and Technology | Shi J.,Tianjin University of Science and Technology | Zheng L.,Tianjin Entry Exit Inspection and Quarantine Bureau
Fuhe Cailiao Xuebao/Acta Materiae Compositae Sinica | Year: 2012

CNTs/PLA composites were prepared by solution blending method in the presence of pristine carbon nanotubes(MWCNTs)and carboxylated carbon nanotubes(MWCNTCOOH). The dispersity of CNTs, crystallization morphology, electric conductivity and degradation were studied. The results show that MWCNTCOOH are dispersed more homogeneously in PLA than the pristine MWCNTs by SEM. POM(Polarizing Optical Microscope) results shows that the MWCNTs act as effective nucleating agents and decrease the crystal size. With the addition of a little MWCNTCOOH, the volume resistivity of the nanocomposite decreases by 7 orders of magnitude, and the degradation also increases by MWCNTCOOH.

Fan M.,Jiangnan University | Zhang P.,Jiangnan University | Zhang Y.,Tianjin Entry Exit Inspection and Quarantine Bureau
Chemical Engineering Communications | Year: 2011

The catalytic synthesis of dipropyl carbonate (DPC) from transesterification of dimethyl carbonate and propyl alcohol over NaY zeolite-supported potassium salt catalysts was investigated at atmospheric pressure. K2CO3/NaY and KOH/NaY catalysts exhibited better catalytic activity than other potassium salt catalysts. From infrared resonance (IR), X-ray powder diffraction (XRD), and X-ray photoelectron spectroscopy (XPS), it could be concluded that K2CO3/NaY and KOH/NaY catalysts showed preferable order degree of zeolite structure, which was associated with higher dispersal of the active species K2O and extraction for part of the non-framework silicon and aluminum of the system after alkali treatment. And the partial extraction of the non-framework silicon and aluminum allowed the reactants and the products to pass through the pore of the catalysts easily and facilitated catalytic performance for DPC synthesis. © Taylor & Francis Group, LLC.

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