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Ma C.-H.,Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory | Guo Z.,Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory
Journal of Interventional Radiology (China) | Year: 2014

Objective: To investigate the inhibitory effect of Endostar combined with cryoablation on lung adenocarcinoma cell line A549 in BALB/c nude mice, and to discuss its interaction mechanisms. Methods: The lung adenocarcinoma A549 model in BALB/c nude mice were established. When the largest diameter of tumor reached 1.0 cm, a total of 24 mice were randomly and equally divided into 4 groups: control group, Endostar group, cryoablation group and cryoablation plus Endostar group. The largest diameter and the vertical diameter of the tumors were measured at different points of time after treatment. At the 21st day, the mice were sacrificed and the tumors were removed and the rate of tumor cell apoptosis, the microvessel density (MVD) and the expression level of vascular endothelial growth factor (VEGF) were determined by using immunohistochemistry method. The results were statistically analyzed. Results: The tumor growth velocity of the control group, Endostar group, cryoablation group and cryoablation plus Endostar group was (236.68 ± 51.23)%, (220.02 ± 30.61)%, (159.46 ± 29.33)% and (103.34 ± 25.50)%, respectively (P < 0.01). The rate of apoptosis of the four groups was (21.67 ± 2.34)%, (22.17 ± 1.47)%, (38.33 ± 1.37)% and (49.17 ± 1.72)%, respectively (P < 0.01). The MVD and the expression levels of VEGF of the cryoablation plus Endostar group were significantly lower than those of the other three groups (P < 0.01). Statistical analysis revealed that a positive correlation existed between the express of VEGF and MVD. Conclusion: Endostar can obviously enhance the therapeutic efficacy of cryoablation on lung adenocarcinoma A549 in BALB/c nude mice. The underlying mechanisms may be the Endostar-inhibited angiogenesis through down-regulating the expression of VEGF, and the cooperative effect of Endostar and cryoablation on the promotion of tumor cell apoptosis.


Zhang Y.,Huanhu Hospital | Zhang Y.,Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory | Gao C.,State Oceanic Administration | Zhao W.,Huanhu Hospital | And 11 more authors.
RSC Advances | Year: 2014

Porous chitosan/gelatin (CS/Gel) foams with ordered porous structures were prepared by a unidirectional freeze-drying method. The CS/Gel foams had ordered porous structures with high porosities. The porosity of the foam depended on the concentration of the CS/Gel preparation solution and the CS/Gel ratio. The CS/Gel concentration affected the compressive strength of the porous CS/Gel foams and higher concentrations resulted in better strength. The CS/Gel ratio affected both the compressive strength and the degradation rate of the CS/Gel foam. Low CS/Gel ratios resulted in foams with higher compressive strength and higher degradation rates. The CS/Gel foams had a high adsorption capacity for aqueous Pb2+ and Co2+ ions and fetal bovine serum. In addition, gentamicin loaded CS/Gel foams had different release rates which depended on the composition and porous structure of the foams. The CS/Gel foams should be useful as scaffolds for cell cultures or drug releasing implants. This journal is © the Partner Organisations 2014.


Zhang Y.,Huanhu Hospital | Zhang Y.,Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory | Yan W.,Huanhu Hospital | Yan W.,Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory | And 7 more authors.
Carbohydrate Polymers | Year: 2015

Ordered porous zeolite/chitosan (Zel/Chi) monoliths were prepared by a unidirectional freeze-drying method, and their properties and structures were characterized by various instrumental methods. The metal ion adsorption and the drug release performance of the porous Zel/Chi monoliths were also studied. The release rate of cefalexin from drug-loaded Zel/Chi monoliths depended on the composition and porous structure of the monoliths. The metal ion adsorption capacity of the Zel/Chi monoliths was related to the concentration of the metal ions, the adsorption time and the Zel/Chi ratio. An experimentally maximum adsorption of 89 mg/g was achieved for Cu2+ ions. The Zel/Chi monoliths with adsorbed Cu2+ ions effectively catalyzed the reduction of 4-nitrophenol to 4-aminophenol and had good recyclability. They were easily recovered by simply removing them from the reaction system and rinsing them with water. © 2014, Elsevier Ltd. All rights reserved.


Zhang Y.,Huanhu Hospital | Zhang Y.,Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory | Yan W.,Huanhu Hospital | Yan W.,Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory | And 4 more authors.
RSC Advances | Year: 2014

A facile and efficient approach was developed to synthesize Ag/Cu@Fe 3O4 hybrid nanoparticles using l-lysine as a linker. The morphology, composition and crystal structure of the prepared Ag/Cu@Fe 3O4 hybrid nanoparticles were characterized by Fourier Transform infrared spectroscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy and powder X-ray diffraction. The magnetic properties of the nanoparticles were determined with a vibrating sample magnetometer. The catalytic activity of the Ag/Cu@Fe3O4 hybrid nanoparticles for the reduction of 4-nitrophenol was also studied. The Ag/Cu@Fe3O4 hybrid nanoparticles are efficient catalysts and can easily be recovered from the reaction system because of their magnetic properties. In addition the Ag/Cu@Fe3O4 catalyst had good reusability. This cost effective and recyclable catalyst provides a new material for use in environmental protection applications. © the Partner Organisations 2014.


Zheng Y.T.,Tianjin Medical University | Kang J.M.,Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory | Liu B.L.,Tianjin Medical University | Fan W.J.,Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory | And 3 more authors.
Chinese Medical Journal | Year: 2014

Background Many researches demonstrate that the secondary brain injury which is caused by autoimmune attack toward brain antigens plays an important role in surgical brain injury (SBI). Although traditional immunosuppression can reduce autoimmune attack, it will lower the body immunity. Immune tolerance, by contrast, not only does not lower the body immunity, but also could lighten autoimmunity. This study used thymus tolerance to develop an immune system that is tolerant to autologous cerebrospinal fluid (CSF) and autologous brain tissue so that autoimmune injury can be suppressed following the disruption of the blood-brain barrier, thereby reducing brain damage. Methods Eighty experimental rabbits were divided into five groups by random number table method: 16 in SBI group (group A), 16 in SBI+CSF drainage group (group B), 16 in SBI+CSF drainage+PBS injection group (group C), 16 in SBI+CSF drainage+CSF intrathymic injection group (group D), and 16 in SBI+brain homogenate intrathymic injection group (group E). Rabbits' CSF was drained in group B; was drained and injected PBS into thymus in group C; was drained and injected CSF into thymus in group D; and was injected brain homogenate in group E. Half of the rabbits in each group were phlebotomized on 1st, 3rd, 7th, and 14th days to observe the changes in IL-l, TGF-β by ELISA test, and CD4CD25 regulatory T cells ratio by flow cytometry, and in other animals brain tissues were taken on 7th day for exploring FasL expression by RT-PCR. The least significant difference (LSD) test was used to make paired comparisons; P <0.05 was considered statistically significant. Results The levels of FasL, TGF-β, and the ratios of CD4CD25 regulatory T cells in groups D and E were apparently higher than those in other three groups (P <0.05). Likewise, the levels of IL-1 in these two groups were lower than the other three groups (P <0.05). Moreover, the ratios of CD4CD25 regulatory T cells and the levels of TGF-β in groups B and C were higher than those in group A, but the level of IL-1 was lower than that in group A (P <0.05). There was no significant difference between groups B and C, and groups D and E. Conclusion Thymic injection of CSF and brain homogenate may be able to reduce inflammation after SBI, so thymus immune tolerance may be a useful therapy to treat SBI.

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