Tianjin Cancer Hospital and Institute

Tianjin, China

Tianjin Cancer Hospital and Institute

Tianjin, China
SEARCH FILTERS
Time filter
Source Type

Dong S.,Guangdong Academy of Medical science | Zhang X.-C.,Guangdong Academy of Medical science | Cheng H.,Sun Yat Sen University | Zhu J.-Q.,Tianjin Cancer Hospital and Institute | And 4 more authors.
Cancer Chemotherapy and Pharmacology | Year: 2012

Purpose Epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) therapy is considered as one of the most important treatments for patients with advanced non-small-cell lung cancer (NSCLC). However, not all patients benefit from this therapy because of primary or acquired resistance, both of which are usually caused by the activation of alternative signaling pathways. Thus, a combination of different signaling pathway inhibitors is a promising strategy. We used the mammalian target of rapamycin (mTOR) inhibitor everolimus in combination with gefitinib in NSCLC cell lines to analyze the efficacy of this combination regimen and the underlying molecular mechanism. Methods Acquired gefitinib-resistant cell lines, together with EGFR wild-Type and mutant primary gefitinib-resistant NSCLC cell lines, were treated with everolimus alone, gefitinib alone, or the combination of the two drugs, and the effects were evaluated using cell proliferation assays. The effects of everolimus and gefitinib on the EGFR pathway in NSCLC cell lines were determined by Western blot analysis. Results Combined treatment resulted in synergistic antitumor effects in gefitinib-resistant cells A549 and H1975. The combination index (CI) of cells increased with increasing dose of everolimus. Everolimus demonstrated no apparent inhibition of phosphorylated Akt (p-Akt) and phosphorylated p44/42 MAPK (p-MAPK) in H1650 cells. Additionally, in gefitinib-resistant cell lines, the combination of gefitinib and everolimus not only showed stronger inhibition of phosphorylated mTOR and phosphorylated p70S6K expression than either drug alone but also reduced the levels of p-Akt and p-MAPK in both cell lines. Conclusions Our data showed that the combination of everolimus and gefitinib exhibits dose-dependent synergism in primary and acquired gefitinib-resistant NSCLC cells. Thus, a preclinical rationale exists for the use of everolimus to enhance the efficacy of gefitinib in EGFRTKI- resistant patients with NSCLC. © Springer-Verlag 2012.


Su Y.-J.,Tianjin Cancer Hospital and Institute | Xu F.,Tianjin Cancer Hospital and Institute | Yu J.-P.,Tianjin Cancer Hospital and Institute | Yue D.-S.,Tianjin Cancer Hospital and Institute | And 2 more authors.
Chinese Medical Journal | Year: 2010

Background S100A8 and S100A9 are two members of the S100 protein family characterized by the presence of two Ca2+-binding sites of the EF-hand type. Previous studies suggested that the whole S100 family displays significant functions in tumor growth, progression and invasion. This study aimed to determine the expression of the two indices of the family, S100A8 and S100A9, in lung cancer tissues and normal lung tissues and its correlation with clinical features. Methods A total of 60 cases with a variety of clinical data that were diagnosed with different histological subtypes of lung cancer were investigated. Semi-quantitative reverse transcriptase-PCR (Sq-Rt-PCR) and immunohistochemical staining of cancer, adjacent and peripheral lung tissues were executed to distinguish the expression patterns of S100A8 and S100A9 and to further clarify their correlation with clinical features. Results Immunohistochemical staining of both proteins showed a significant up-regulation in lung cancer tissue (S100A8, S100A9, P <0.0001), and PCR revealed that the levels of S100A8 and S100A9 expression were significantly higher in lung cancer tissues (S100A8 P=0.002/0.004; S100A9 P=0.022/0.026). The higher expression was found to be correlated with the clinical characteristics of adenocarcinoma, inflammation and stage IV lesion. Conclusions S100A8, S100A9 up-regulation was found in the lung adenocarcinoma and end stage lung cancer tissue, the correlation of which with their higher expression in inflammatory lung tissues may indicate the collaborative effect of inflammation on the progression of cancer.


Chen J.,Tianjin Cancer Hospital and Institute
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi | Year: 2012

Temperature scanning tests and uniaxial tensile tests at room temperature under different strain rates for three kinds of existing low-temperature outside fixed thermoplastic medical polymer resin materials (A, B and C) were conducted on dynamic mechanical analyzer and micro material testing machine to contrast their mechanical properties. It is shown that the glass transition temperatures of these three materials are all around 65 degrees C, but material C has the widest range of shaping temperature. Stress-strain responses of the three materials are rate-dependent and material C shows better plasticity.


PubMed | Tianjin Cancer Hospital and Institute
Type: Journal Article | Journal: Chinese medical journal | Year: 2010

S100A8 and S100A9 are two members of the S100 protein family characterized by the presence of two Ca2+-binding sites of the EF-hand type. Previous studies suggested that the whole S100 family displays significant functions in tumor growth, progression and invasion. This study aimed to determine the expression of the two indices of the family, S100A8 and S100A9, in lung cancer tissues and normal lung tissues and its correlation with clinical features.A total of 60 cases with a variety of clinical data that were diagnosed with different histological subtypes of lung cancer were investigated. Semi-quantitative reverse transcriptase-PCR (Sq-Rt-PCR) and immunohistochemical staining of cancer, adjacent and peripheral lung tissues were executed to distinguish the expression patterns of S100A8 and S100A9 and to further clarify their correlation with clinical features.Immunohistochemical staining of both proteins showed a significant up-regulation in lung cancer tissue (S100A8, S100A9, P<0.0001), and PCR revealed that the levels of S100A8 and S100A9 expression were significantly higher in lung cancer tissues (S100A8 P=0.002/0.004; S100A9 P=0.022/0.026). The higher expression was found to be correlated with the clinical characteristics of adenocarcinoma, inflammation and stage IV lesion.S100A8, S100A9 up-regulation was found in the lung adenocarcinoma and end stage lung cancer tissue, the correlation of which with their higher expression in inflammatory lung tissues may indicate the collaborative effect of inflammation on the progression of cancer.


Wang J.-j.,Tianjin Cancer Hospital and Institute | Zhang Y.-z.,Tianjin Cancer Hospital and Institute | Yu Y.,Tianjin Cancer Hospital and Institute | Wang X.-f.,Tianjin Cancer Hospital and Institute | And 3 more authors.
Journal of Leukemia and Lymphoma | Year: 2011

Objective To improve the understanding of chronic myelomonocytic leukemia associated with Sweet ′s syndrome. Methods Retrospective analysis of a case of chronic myelomonocytic leukemia associated with skin herpes was reported. Skin biopsy was performed. DA and CAG regiment were administrated. Results Sweet ′s syndrome was diagnosed by skin biopsy. Corticosteroids therapy alone was not effective. Complete remission was achieved by CAG regiment and skin rash has been effectively controlled. Three months later Sweet ′s syndrome relapsed and chronic myelomonocytic leukemia developed into acute myelomonocytic leukemia. Conclusion Chronic myelomonocytic leukemia associated with Sweet ′s syndrome is rare but implies a quick progression to acute myelomonocytic leukemia.


PubMed | Tianjin Cancer Hospital and Institute
Type: Journal Article | Journal: Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi | Year: 2012

Temperature scanning tests and uniaxial tensile tests at room temperature under different strain rates for three kinds of existing low-temperature outside fixed thermoplastic medical polymer resin materials (A, B and C) were conducted on dynamic mechanical analyzer and micro material testing machine to contrast their mechanical properties. It is shown that the glass transition temperatures of these three materials are all around 65 degrees C, but material C has the widest range of shaping temperature. Stress-strain responses of the three materials are rate-dependent and material C shows better plasticity.

Loading Tianjin Cancer Hospital and Institute collaborators
Loading Tianjin Cancer Hospital and Institute collaborators