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Liu X.,Fifth Central Hospital of Tianjin | Liu X.,Tianjin Binhai Neurological Institute | Li G.,Fifth Central Hospital of Tianjin | Su Z.,Fifth Central Hospital of Tianjin | And 10 more authors.
Oncology Reports | Year: 2013

microRNAs are regarded as promising drugs for glioma gene therapy. However, conventional administration routes, such as oral administration and intravenous infusion, present low efficiency due to the blood-brain barrier and intercellular retention, thereby limiting their application. Recent studies showed poly(amido amine) (PAMAM) was a candidate carrier due to its high solubilization, delayed release and low toxicity. In the present study, U251 human brain glioma cells were transfected with the miR-7 gene using PAMAM as the vector to determine the transfection efficiency and therapeutic effects in vivo and in vitro. We found that PAMAM exhibited higher transfection efficiency and longer duration of action compared with liposome delivery, and miR-7 efficiently silenced some genes involved in the epidermal growth factor receptor (EGFR) pathway and achieved favorable effects in treating glioma in vivo and in vitro. These investigations provide a basis for developing high-efficiency micromolecular drug delivery. © 2013 Spandidos Publications Ltd. All rights reserved. Source


Liu X.,Tianjin Binhai Neurological Institute | Chen L.,Tianjin Binhai Neurological Institute | Jiang Z.,The Fifth Central Hospital of Tianjin | Wang J.,Tianjin Binhai Neurological Institute | And 6 more authors.
Experimental and Therapeutic Medicine | Year: 2013

Following emergence of the tumor stem cell theory, the increasing number of related studies demonstrates the theory's growing importance in cancer research and its potential for clinical applications. Few studies have addressed the in vitro or in vivo properties of glioma stem cells from a Han Chinese population. In the present study, surgically obtained glioblastoma tissue was classifed into two subtypes, CD133+ and CD133-. The hierarchy, invasiveness, growth tolerance under low nutrient conditions and colony forming abilities of the tissue samples were analyzed. Additionally, the characteristics of tumor cells transplanted subcutaneously or re-transplanted into nude mice were observed. The results demonstrated that CD133+ glioblastoma cells derived from Han Chinese glioma specimens were more prone to primitive cell differentiation and more invasive than CD133- glioblas-toma cells, leading to increased tumor malignancy compared with CD133- cells. The tumor formation rates of CD133+ and CD133- cells in mice were 26/30 and 2/30, respectively. A comparison of tumor subtypes demonstrated that CD133+ glioblastoma cells had a lower incidence of cell apoptosis in the tumor tissue and higher protein expression levels of Oct4, Sox2, PCNA, EGFR, Ang2, MMP2 and MMP9 compared wit h C D133 - cells. Flow cytometry revealed that in the CD133+ and CD133- glioblastoma cell-induced tumors, the percentage of CD133+ cells was 2.47±0.67 and 0.44±0.14%, respectively. The tumor formation rates following the re-transplantation o f C D13 3 + o r C D133 - tumors into nude mice were 10/10 a nd 4/10, respectively. These findings suggest that the CD133+glioblastoma cell subpopulation has a stronger malignant cell phenotype than the CD133- subpopulation and that its recurrence rate is increased compared with the primitive tumorigenic rate following in vivo transplantation. Source

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