Tianjin Agricultural College
Tianjin Agricultural College
Yang H.-P.,Tianjin Agricultural College |
Que S.,Qinghai Normal University |
Shi Y.-P.,CAS Lanzhou Institute of Chemical Physics |
Ban L.-T.,Tianjin Agricultural College
Journal of Chemical and Pharmaceutical Research | Year: 2014
A thorough investigation of the flower part of Gentiana veithiorum afforded one new triterpene, 3□, 11β-dihydroxyurs-12-en-28-oic acid (1), as well as nine known triterpenoids, ursolic acid (2), 3β, 28-dihydroxyurs-12-ene, (3), Urs-12-en-28-hydroxy-3β-O- palmitate (4), α-amyrin (5), 2α, 3β-dihydroxyurs-12-en-28-oic acid (6), oleanolic acid (7), Lup-28-hydroxy -3β-O- palmitate (8), lupeol (9), 3-epi-betulinic (10).The structures of the new compound were characterized by means of spectroscopic methods in cluding 1D, 2D NMR, ESI-MS and HRESI- MS, and the known ones were established on the basis of com- paring their NMR data with those of the corresponding compounds in the literature.Above all compounds were found in this plant for the first time. © 2014, Journal of Chemical and Pharmaceutical Research. All rights reserved.
Zhu H.,Tianjin Agricultural College |
Chen C.,Tianjin Agricultural College |
Wei Z.,Tianjin Agricultural College |
Tian L.,Tianjin Agricultural College
Asian Journal of Chemistry | Year: 2013
Manganese(II), nickel(II), copper(II) and zinc(II) complexes were prepared by the reactions of the correspondence metal acetate and the hydrazone ligands (L1-L3) derived from 1-phenyl-3-methyl-4-acyl-pyrazole and benzoyl hydrazine. The ligands (L1-L3) and metal complexes were characterized by elemental analysis and spectroscopic techniques such as 1H NMR and IR. Antimicrobial activity studies of (L 1-L3) and the metal complexes against Escherichia coli and Bacillus subtilis were carried out by using disc diffusion method. The results indicate that the free ligands are more or less inactive against the two bacteria, the antibacterial abilities of the ligands become more pronounced when they are coordinated to the metal ions. The copper(II) complex of the benzoic acid [1-(5-methyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4- yl)ethylidene] hydrazide (L1) shows a signiûcant inhibition to the growth of the two tested bacteria.
Yang H.,Academy of Military Medical science |
Yang H.,Tianjin Agricultural College |
Pang W.,Academy of Military Medical science |
Lu H.,Academy of Military Medical science |
And 3 more authors.
Journal of Agricultural and Food Chemistry | Year: 2011
The metabonomics changes of plasma and brain tissue after dietary supplementation with blueberry extracts (BBE) and cyanidin-3-O-galactoside from blueberry (BBM) in aged mice were investigated by 1H NMR technique. The mice received intragastric administration of BBE (200 mg/kg/day), BBM (50 mg/kg/day), and saline water (0.9%) for 6 weeks, respectively, in the BBE, BBM, and control groups. At the end of the experiment, plasma and brain samples were collected for NMR analysis. The results demonstrated that the level of choline in plasma from BBE and BBM groups were obviously elevated relative to the control group, whereas the levels of lactate and phosphocholine in plasma were remarkably reduced. Compared with those in the control group, the levels of choline and GABA in the brain from the BBE group were obviously increased, whereas glutamate and phosphocholine in the BBE group were significantly decreased. The level of taurine in the brain from the BBM group was particularly higher than that in the control group. These results indicated supplementation with BBE or BBM might induce similar changes of endogenous plasma and brain metabolic profiles in aged mice. © 2011 American Chemical Society.
Lu S.,China Agricultural University |
Lu S.,Tianjin Agricultural College |
Yan Z.,China Agricultural University |
Chen Q.,China Agricultural University |
Zhang F.,China Agricultural University
Journal of Plant Nutrition | Year: 2012
Conventional practices of nitrogen (N) and phosphorus (P) application were evaluated based on nutrient balance calculation and soil N and P level from 916 surveyed orchards in North China, in order to assess the potential environmental risks. The results showed that excessive N and P application was common, with the average input rates of 588.4 kg N ha-1, and 156.7 kg P ha-1, respectively, which were 2.5-3.0 folds higher than the fruit N and P demand. High proportions of surplus N and P were found in grape, apple, pear and peach orchards in the plain regions with high economic returns. Nitrogen surplus reduced soil carbon (C)/N ratio in the plain, plateau and mountain regions. High soil Olsen-P level in the 40% of surveyed orchards, predominantly distributed in grape and peach orchards, was over the environmental threshold. Controlling N and P fertilization is the key to maintain sustainable fruit production in North China. © 2012 Copyright Taylor and Francis Group, LLC.
Gai Y.,CAS Qingdao Institute of Oceanology |
Gai Y.,University of Chinese Academy of Sciences |
Wang L.,CAS Qingdao Institute of Oceanology |
Song L.,CAS Qingdao Institute of Oceanology |
And 3 more authors.
Fish and Shellfish Immunology | Year: 2010
Insulin growth factor binding proteins (IGFBPs), characterized by the conserved insulin binding (IB) domains, are important components of endocrine system and play key roles in metabolism and growth. In the present study, the full-length cDNA of a single IB domain protein (designated EsSIBD) was identified from Chinese mitten crab Eriocheir sinensis based on expressed sequence tag (EST) sequence. The 1187 bp EsSIBD cDNA contained a 321 bp open reading frame (ORF) encoding a putative protein of 106 amino acids, a 5′-untranslated region (UTR) of 189 bp, and a 3′-UTR of 677 bp. Multiple sequence alignment presented ten conserved cysteine residues critical for the fundamental structure and function of IB domain. BLAST analysis revealed that EsSIBD shared high similarity with previously known IB domains of IGFBPs with the identities ranging from 40% to 46%. The sequence similarity and domain conservation indicated that EsSIBD was a potential member of the IGFBP family. Phylogenic analysis presented that EsSIBD was closer to IGFBP7 than to the other IB domain containing proteins, suggesting its functional similarity with the endocrine factor IFGBP7. The mRNA expression of EsSIBD in different tissues including hepatopancreas, gill, gonad, muscle, heart and haemocytes, and the temporal expression in haemocytes challenged by Listonella anguillarum were measured by real-time RT-PCR. EsSIBD mRNA transcripts could be detected in all examined tissues with the highest expression level in gill. The EsSIBD mRNA expression in haemocytes was sensitive to L. anguillarum stimulation and it was up-regulated from 3 to 24 h after challenge. The structure conservation and functional similarity to IFGBPs, and its sensitivity to L. anguillarum stimulation collectively implied that EsSIBD was probably involved in endocrine and immune systems of Chinese mitten crab, and provided insight into the cross-talk between the invertebrate endocrine and immune system. © 2009 Elsevier Ltd. All rights reserved.
PubMed | Tianjin Hospital, Yantai Economic and Technological Development Area Hospital, Tianjin Medical University, Academy of Military Medical science and Tianjin Agricultural College
Type: | Journal: Journal of Alzheimer's disease : JAD | Year: 2016
Blueberry (BB) can provide a wide range of antioxidant benefits for AD. There have amounts of evidences shown that BB extracts could improve brain functions. However, the details are still unknown.In the present study, we aimed to investigate the possible mechanism involved in the improvement of learning and memory capacity from BB extracts in AD.APP/PS1 transgenic mice were fed BB extracts for 16 weeks. The capacity of learning and memory was assessed by Morris water maze (MWM) test, and long-term potentiation (LTP) was determined to evaluate hippocampal neuronal plasticity at the end of administration. Pathological changes in the brain were observed, and the expressions of brain-derived neurotrophic factor (BDNF) and extracellular signal-related kinase (ERK1/2) were determined to explore the mechanism of BB extract-induced benefits.AD mice exhibited more difficulties to learn and remember the exact position of platform in the MWM test. The data showed that AD mice lacked effective learning in the platform search. In contrast, AD mice exhibited better performance both in the training phase and probe test of MWM after the BB treatment. Moreover, LTP was enhanced and the neuron loss was alleviated with BB treatment, while we did not find any obvious effect on the elimination of amyloid-. In the AD mice, the expression of ERK1/2 was significantly increased (p<0.05), while the level of BDNF was decreased (p<0.05).BB treatment was beneficial for the improvement of learning and memory of AD, and these effects might be related to the regulation of BDNF.
Shan H.,Tianjin Agricultural College |
Shan H.,Tianjin University |
Wang T.,Tianjin University |
Yang Y.,Tianjin Agricultural College
Jixie Qiangdu/Journal of Mechanical Strength | Year: 2011
The design of the magnetic circuit is a very important step in the whole design steps of MR(magnetorheological) clutch. The magnetic circuit material selection, magnetic circuit structure and electromagnetic coil about the magnetic circuit design are systematical introduced. Based on the analysis of influence factors to magnetic circuit design, the method of magnetic circuit design to avoid the magnetic saturation was put forward. And the magnetic circuit Ohm's law was applied in calculating the magnetometive force of magnetic circuit. The calculation procedures were described in detail. The method of the calculation of electro-magnetic coil parameters was presented. Finally, application of the research results to the magnetic circuit design of disc-type MR clutch was completed.
Cheng Z.,Texas A&M University |
Cheng Z.,Tianjin Agricultural College |
Buentello A.,Texas A&M University |
Gatlin D.M.,Texas A&M University
Aquaculture | Year: 2011
Both arginine and glutamine play important roles in tissue repair, cell replication and collagen synthesis which impact animal growth and survival. Thus, a 7-week feeding trial was conducted to determine the effects of dietary arginine and glutamine on growth performance, innate immune responses and intestinal structure of juvenile red drum, Sciaenops ocellatus (6.9 ± 0.04. g, initial average weight). Protein in all experimental diets was derived from menhaden fishmeal and dehulled, solvent-extracted soybean meal to provide isonitrogenous (44% crude protein) and isolipidic (10%) diets. The basal diet contained 0.14% glutamine and 1.72% arginine on a dry-matter basis, which exceeds the established dietary requirement of red drum. Experimental diets were prepared by supplementing the basal diet with either arginine at 1% of dry weight, glutamine at 1%, glutamine at 2%, or both arginine and glutamine at 1%, with adjustments in glycine to maintain equal nitrogen among all diets.Feed efficiency was significantly (P< 0.05) improved by supplementation of glutamine at 2% and the combination of both arginine and glutamine at 1% of diet. Neutrophil oxidative radical production in fish fed the glutamine and/or arginine-supplemented diets was significantly (P= 0.03) higher compared with that of fish fed the basal diet, with a synergistic effect observed in fish fed the combined arginine-glutamine diet. Significantly (P< 0.05) higher serum lysozyme activity also was observed in fish fed the diet supplemented with 1% of both arginine and glutamine. Extracellular superoxide anion production by red drum macrophages was significantly (P< 0.05) higher for fish fed diets with glutamine at either 1 or 2% and the 1% arginine-glutamine combination compared to fish fed the basal diet. Similarly, macrophages from fish fed the diet with glutamine at 2% and the arginine-glutamine combination at 1% produced significantly higher amounts of intracellular superoxide anion.Morphometric analyses - i.e., measurements of enterocyte, microvillus and fold heights - demonstrated positive effects of both dietary glutamine and arginine in different portions (proximal, mid and distal) of the gastrointestinal tract of red drum. In general, fish fed the diet supplemented with 2% glutamine had the greatest increases in magnitude of the chosen structures, although those fed the diet with arginine at 1% also yielded improved scores for some enteric portions. Therefore, results from the present study establish the importance of both dietary arginine and glutamine supplementation in improving feed efficiency, as well as eliciting positive changes to several components of the innate immune system and intestinal functionality of red drum. © 2011 Elsevier B.V.
Yan Z.-L.,Tianjin Agricultural College |
Shi J.,Tianjin Agricultural College
Asian Journal of Chemistry | Year: 2012
Ce 0.9Pr 0.1O 2-d mixed oxide was prepared by citrate sol-gel, decomposition of nitrate and coprecipitation methods. The effect of preparation methods on catalytic activity for CO has been investigated. The mixed oxide prepared by citrate sol-gel method has the best CO oxidation activity.
Liu X.,Tianjin University of Science and Technology |
Li X.,Tianjin University of Science and Technology |
Zhang X.,Tianjin Agricultural College |
Wang S.,Tianjin Agricultural College
Genome | Year: 2010
Thermosensitive genic male-sterile (TGMS) rice is widely used in hybrid rice production. Because of its specific temperature requirement, it can be used only in a narrow rice-growing zone in Asia. A newly discovered TGMS line, G20S, has an opposite phenotype compared with normal TGMS lines. G20S is completely sterile when the temperature is lower than 29.5 8C. Thus, it can be widely used in a larger area. Genetic analysis indicated that the sterility of G20S was controlled by a single recessive gene that was named tms6(t). An F2 population from the cross between G20S and Jing226 was developed and used for molecular mapping of the tms6(t) gene. The simple sequence repeat technique, combined with bulked segregant analysis, was used to screen markers linked to the target gene, and linkage markers such as RM216, RM2504, RM3152, and RM4455 were identified. These markers are all located on chromosome 10; therefore, tms6(t) was subsequently mapped on chromosome 10. Co-segregation analysis using the F2 population showed that two of these markers, RM3152 and RM4455, were closely linked to tms6(t) at distances of 3.00 cM and 1.10 cM, respectively. Additionally, integration of the genetic and physical maps showed that there were 15 BAC/PAC clones overlapping between RM3152 and RM4455, and tms6(t) was mapped to an interval of 1455 kb.