Sun B.,Shandong University |
Xu H.,Tianjin Academy of Traditional Chinese Medicine |
Zhang G.,First Peoples Hospital of Zibo City |
Sun H.,Shandong University |
Hou G.,Shandong University
Oncology Reports | Year: 2013
Basic fibroblast growth factor (bFGF) plays an important role in tumor angiogenesis. Several studies have reported that bFGF may influence cell apoptosis through different signaling pathways. The aim of the present investigation was to study the effect of bFGF on the activities of protein kinase B (PKB)/survivin and cell apoptosis in hepatocellular carcinoma cells (Bel-7402). We treated Bel-7402 cells with bFGF and wortmannin [phosphatidylinositol 3-kinase (PI3K)-specific inhibitor] separately to observe the expression of PKB and survivin detected with RT-PCR and western blotting. The cell cycle and apoptosis were assayed with flow cytometry. We found a significant increase in PKB expression in the group treated with 25 ng/ml bFGF for 10 min (P<0.05), and this effect was significantly inhibited by pretreatment with wortmannin (200 nM) for 1 h. After treatment with 10 ng/ml bFGF, the expression of survivin mRNA in Bel-7402 cells increased significantly, and reached the peak at 16 h (P<0.05); however, this effect could be significantly inhibited by pretreatment with wortmannin (200 mM) in a time-dependent manner. Following incubation with 25 ng/ml bFGF for 10 min, the apoptosis rate and M phase were significantly decreased and S phase cells increased compared with the wortmannin (200 nM)-treated group. When this group was pretreated with wortmannin (200 nM) for 1 h, the apoptosis rate and S phase were significantly increased, M phase cells decreased. The results revealed that wortmannin could induce high apoptosis rates in hepatocellular carcinoma cells, and bFGF could inhibit the cell apoptosis induced by wortmannin. These findings indicate that bFGF could rapidly activate the PKB activities, enhance the expression of survivin and the proliferation of hepatocellular carcinoma cells via the PI3K pathway, thus it may serve as a novel molecule for early targeting therapy of hepatocellular carcinoma.
Wang H.,Tianjin Nankai Hospital |
Qian Z.,Tianjin Hospital |
Zhao H.,International Travel Healthcare Center |
Zhang X.,Tianjin Nankai Hospital |
And 7 more authors.
Molecular Medicine Reports | Year: 2015
Hepatocellular carcinoma (HCC) is one of the most common tumor types, and is the third leading cause of cancer mortalities worldwide. A large number of patients with HCC are diagnosed at a late stage when the curative treatment of surgical resection and liver transplantation are no longer applicable. Sorafenib has been proved to improve overall survival in advanced HCC; however, drug resistance is common. The present study reported that the CSN5 is correlated with sorafenib resistance of the HCC cell line HepG2/S. Following silencing of CSN5, resistance to sorafenib was reversed, and multi-drug-resistance proteins, including as adenosine triphosphate binding cassette (ABC) B1, ABCC2 and ABCG2 as well as CDK6, cyclin D1 and B-cell lymphoma 2 were downregulated. In addition, it was demonstrated that the integrin beta-1, transforming growth factor-β1 and nuclear factor-κB pathways were modified by CSN5.
Zuo C.,Tianjin Police Hospital |
Tan X.,Nankai University |
Jia S.,Tianjin Police Hospital |
Zhang M.,Tianjin Police Hospital |
Zhang D.,Tianjin Academy of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine | Year: 2014
OBJECTIVE: To observe the effects and mechanisms of Bushenhuoxue on desmin and nephrin expression in mice podocytes, and to investigate its effects on wt1 expression in Wilms' tumor.METHODS: Adriamycin (ADR) was used to induce focal segmental glomerulous sclerosis (FSGS) in mice. Bushenhuoxue was used to treat FSGS for 6 weeks. We measured body mass and right renal mass, and determined serum albumin (ALB) levels, protein content in urine, and urinary protein and albumin creatinine ratio (UACR). Changes in renal tissue morphology were evaluated by microscopy. wt1 and nephrin expression in podocytes were detected using immunofluorescence. Expression levels of desmin, wt1 and nephrin mRNAs in renal tissue were determined using reverse transcription polymerase chain reaction assays.RESULTS: Protein levels in urine and UACR were significantly increased in FSGS model mice compared with Bushenhuoxue-treated and control mice. Body mass and ALB levels were decreased in FSGS mice compared with control and Bushenhuoxue-treated mice. Expression of the wt1 protein was observed in control mice. Compared with controls, wt1 expression levels were reduced in Bushenhuoxue-treated mice, and to a greater extent in FSGS mice. Nephrin protein expression was widespread in FSGS mice, and significantly reduced in control and Bushenhuoxue mice. Expression levels of wt1 and nephrin mRNAs in FSGS mice were lower compared with those in control and Bushenhuoxue-treated mice. Desmin mRNA levels in FSGS mice were reduced compared with those in control and Bushenhuoxue-treated mice.CONCLUSION: Bushenhuoxue ameliorated albuminuria in FSGS mice; this was possibly related to the up-regulation of wt1 and nephrin, and down-regulation of desmin. © 2014 JTCM. All rights reserved.
Zhang H.-Y.,Xinjiang Medical University |
Zhao Y.,Xinjiang Medical University |
Xie C.,Xinjiang Medical University |
Cheng Y.-T.,Xinjiang Medical University |
Wang C.-C.,Tianjin Academy of Traditional Chinese Medicine
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: Currently, the treatment of proximal humeral fractures mainly contains joint preservation (conservative treatment, open reduction and internal fixation) and arthroplasty, but how to choose the treatment is still controversial. OBJECTIVE: To evaluate and compare the clinical outcomes of joint preservation versus arthroplasty in the treatment of displaced 3-or 4-part humeral fractures in randomized controlled trials using meta-analysis. METHODS: Medline (January 1966 to December 2013), PubMed (January 1980 to December 2013), Embase (January 1990 to December 2013), Science (January 1990 to December 2013), Springer (January 1990 to December 2013), China National Knowledge Infrastructure (1994 to 2013), and Wanfang database (1982 to 2013) were searched for randomized controlled trials addressing joint preservation and arthroplasty for 3-or 4-part proximal humeral fractures. Articles meeting the inclusion criteria were included. The related data were extracted and loaded onto Comprehensive Meta-Analysis Software for meta-analysis. RESULTS AND CONCLUSION: Seven articles with 320 patients (165 patients undergoing joint preservation and 155 patients receiving arthroplasty) were accepted in this mete-analysis and all of them were high-quality English researches by modified Jadad Scale. Meta-analysis results displayed that the random-effects mean Constant score across all types was 55.9 (95%CI: 50.7-61.1; P < 0.001). Constant score was higher in the joint preservation group than in the arthroplasty group (P < 0.01). The study displayed significant heterogeneity (I2=88%, Q statistic=107.6, Q=13; P < 0.001). In the meta-analysis, Constant scores were decreased with increasing rate of male to female and rate of tuberosity resorption. These results suggested that 3-or 4-part proximal humeral fractures demonstrate improved Constant scores when treated with joint-preserving options compared with arthroplasty. Moreover, age, fracture pattern, gender ratio and complication rate are significant predictors of the Constant score. Given the observed heterogeneity and variance in treatment techniques in the included studies, more randomized controlled trial studies are needed to definitively recommend joint-preserving techniques versus arthroplasty for treatment of 3- or 4-part proximal humeral fractures.
Zhang Y.,Tianjin Academy of Traditional Chinese Medicine |
Li H.,Tianjin Academy of Traditional Chinese Medicine |
Qiao S.-F.,Tianjin Academy of Traditional Chinese Medicine
Journal of Clinical Dermatology | Year: 2013
A 64-year-old female had suffered from muscle pain and weakness for 4 months, generalized erythemas and vesiculo-bullous lesions on the forearms and dysphagia for 2 months. Pathological examination showed vesicles below epidermis and negative for direct immunofluorescence staining. Esophageal biopsy revealed esophageal carcinoma. She died 2 months later. The diagnosis was dermatomyositis and esophageal carcinoma. The clinical features of this case are summarized and literatures on vesiculo-bullous dermatomyositis are reviewed.
Gu A.-K.,Tianjin Academy of Traditional Chinese Medicine |
Xiao Y.,Tianjin Academy of Traditional Chinese Medicine |
Ji H.-A.,Tianjin Academy of Traditional Chinese Medicine
Journal of Clinical Dermatology | Year: 2013
A case of syringoid eccrine carcinoma is reported. A 56-year-old woman presented a painful, skin-colored plaque on the left ear with a size of 4 cm x 3 cm for 10 years. The lesion was firm, smooth, tender, and fixed to the underlying tissue, without ulceration. Histopathology revealed clusters of basaloid tumor cells in the middle and lower dermis and subcutaneous adipose tissue, arranged in cords, nests and tadpole-like form, with tubulocystic formation and fibrous stroma proliferation, and separated from the tumor cells. Either keratinous cyst or sieve-like structure was not seen. Immunohistochemistry study showed positive staining for epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA), cytokeratin (CK)5.2, CK7, CK5/6 and S-100, and negative staining for thyroid transcription factor-1(TTF-1), CK20 and gross cystic disease fluid protein 15 (GCDFP 15). The diagnosis was made as syringoid eccrine carcinoma.
Wang J.P.,Tianjin Academy of Traditional Chinese Medicine
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2013
To observe the clinical effect and efficacy of Xiaozhi Capsule (XZC), a Chinese medicine preparation for tonifying Gan-Shen, invigorating Pi to dissipate dampness (TGSIPDD) on total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and endothelin (ET) in treating patients with hyperlipidemia. Totally 120 primary hyperlipidemia patients were randomly assigned to the treatment group (80 cases) and the control group (40 cases). Those in the treatment group took XZC, while those in the control group took Xuezhikang Capsule (XZKC). The serum TC, TG, HDL-C, LDL-C, and ET were detected and evaluated after 8 weeks of treatment. In the treatment group TC was reduced by 25.60%, TG by 33.70%, LDL-C by 32.90%, and ET by 11.02%, while HDL-C was elevated by 24.20%. In the control group, TC was reduced by 24.80%, TG by 33.50%, LDL-C by 31.30%, and ET by 12.05%, while HDL-C was elevated by 20.90%. There was statistical difference in the two groups when compared with before treatment (P < 0.01). But there was no statistical difference in the aforesaid indices between the two groups after treatment (P > 0.05). The integrals for main symptoms after treatment obviously decreased in the two groups, showing statistical difference when compared with before treatment in the same group (P < 0.01). But there was no statistical difference in the aforesaid indices between the two groups (P > 0.05). After 8 weeks of treatment, symptoms such as vertigo, heavy sensation of head, palpitation, chest distress, dry mouth and thirsty were obviously improved after treatment. There was statistical difference in the improvement of tinnitus after treatment in the treatment group (P < 0.01). The total effective rate was 86.25% in the treatment group and 82.50% in the control group, showing no statistical difference (P > 0.05). XZC showed certain effects on each blood lipid index and ET of hyperlipidemia patients. It had better improvement of clinical symptoms with reliable efficacy.
Li Q.,Tianjin Medical University |
Li Q.,Tianjin University |
Bao J.-M.,Tianjin University |
Li X.-L.,Tianjin Academy of Traditional Chinese Medicine |
And 2 more authors.
Chinese Medical Journal | Year: 2012
Background Astragalus polysaccharides (APS), the main active extract from Astragalus membranaceus (a traditional Chinese medicinal herb), is associated with a variety of immunomodulatory activities. The purpose of the present study was to examine the effect of APS on the function of Treg cells in the tumor microenvironment of human hepatocellular carcinoma (HCC) and to identify the pharmacologic mechanism of APS responsible for the anti-chemotactic activity in CD4 +CD25 highTreg cells in tumor site of HCC. Methods The prevalence of Treg in fresh tissue samples from 31 patients with HCC after radicalhepatectomy was detected. CD4, CD25 and CD127 were selected as Treg cell makers to phenotype cell populations. The expression of FOXp3 mRNA was also analyzed. The migration and proliferation of Treg cells were observed. Interleukin (IL)-4, IL-10, IFN-γ and SDF-1 in cell supernatant were detected. For all tests, functions of Treg cells were evaluated after treatment with APS. Results APS can inhibit the growth and proliferation of CD4 +CD25 +Treg cells in vitro in a dose- and time-dependent manner. APS may inhibit CD4 +CD25 +Treg cells through restoring the cytokine imbalance and reducing the expression of FOXp3 in local HCC microenvironments. SDF-1 played an important role in there recruitment of Treg cells into the tumor microenvironment of HCC. APS might have inhibiting effects on Treg cell migration by blocking SDF-1 or its receptor through the CXCR4/CXCL12 pathway. Conclusions The increase in numbers of tumor associated Treg cells might play a role in modulation of the immune response against HCC. APS can restore the cytokine balance in the tumor micro environment and suppress the expression of FOXp3 mRNA to inhibit the immune suppressive effects of Treg cells. The application of APS in the tumor microenvironment might act to enhance the anti-tumor effects of the immunotherapy-based methods, and consequently to increase the survival rate in HCC.