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Liu M.,China Institute of Technology | Ning B.,China Institute of Technology | Qu L.,Huazhong Agricultural University | Peng Y.,China Institute of Technology | And 5 more authors.
Sensors and Actuators, B: Chemical | Year: 2012

In this work, we demonstrate the development of a highly sensitive and selective surface plasmon resonance (SPR) immunosensor for label-free detection of ractopamine (RCT) as low as 0.12 ppb (ng mL -1) direct from pork liver samples. RCT is a kind of the β-adrenergic agonist with N-alkylphenyl substituent, and the development of high performance sensing methods for RCT is a critical issue because of its vital role in human metabolism. To construct the immunosensor system, the biosensor surface was formed by immobilization of ractopamine-ovalbumin (RCT-OVA) conjugate onto an Au-thiolate self-assembled SPR sensor chip by covalent amide binding, and the detection of RCT was performed based on indirect competitive inhibition principle. Detailed works were carried out focusing on optimizing the conditions that affect immunosensitivity, including immobilization buffer, immunobinding buffer, RCT-OVA concentration, antibody concentration, activation time, regeneration buffer, etc. A preferable way to determine the optimal antibody concentration was performed comparing with that used in most other reported researches. Under the optimal conditions, the biosensor exhibits a linear detection range of 0.28-4.29 ng mL -1, LOD of 0.12 ng mL -1 and IC 50 of 1.17 ng mL -1. It improved that the developed SPR immunosensor is more sensitive for detection of RCT against other reported ones. © 2011 Elsevier B.V. All rights reserved.


PubMed | Nankai University, Shanxi Agricultural University, Howard University, China Institute of Technology and 2 more.
Type: | Journal: BMC plant biology | Year: 2016

It is widely accepted that cultivated rice (Oryza sativa L.) was domesticated from common wild rice (Oryza rufipogon Griff.). Compared to other studies which concentrate on rice origin, this study is to genetically elucidate the substantially phenotypic and physiological changes from wild rice to cultivated rice at the whole genome level.Instead of comparing two assembled genomes, this study directly compared the Dongxiang wild rice (DXWR) Illumina sequencing reads with the Nipponbare (O. sativa) complete genome without assembly of the DXWR genome. Based on the results from the comparative genomics analysis, structural variations (SVs) between DXWR and Nipponbare were determined to locate deleted genes which could have been acquired by Nipponbare during rice domestication. To overcome the limit of the SV detection, the DXWR transcriptome was also sequenced and compared with the Nipponbare transcriptome to discover the genes which could have been lost in DXWR during domestication. Both 1591 Nipponbare-acquired genes and 206 DXWR-lost transcripts were further analyzed using annotations from multiple sources. The NGS data are available in the NCBI SRA database with ID SRP070627.These results help better understanding the domestication from wild rice to cultivated rice at the whole genome level and provide a genomic data resource for rice genetic research or breeding. One finding confirmed transposable elements contribute greatly to the genome evolution from wild rice to cultivated rice. Another finding suggested the photophosphorylation and oxidative phosphorylation system in cultivated rice could have adapted to environmental changes simultaneously during domestication.


Meng Z.,Shanghai JiaoTong University | Shi H.,Tianjin Academy of Agricultural science
International Journal of Clinical and Experimental Medicine | Year: 2016

This study was to analysis the correlation between genetic polymorphisms in antioxidant enzyme genes and the susceptibility factors to hepatocellular carcinoma (HCC) using meta analysis. Electronic searches were conducted in Pubmed, Embase and Cochrane library. Retrieval keywords included antioxidant enzyme, superoxide dismutases, catalase, glutathione peroxidase, and hepatocellular carcinoma. Retrieval strategy was: ((antioxidant enzyme) OR (superoxide dismutases) OR catalase OR (glutathione peroxidase)) AND (hepatocellular carcinoma) OR HCC) AND polymorphism (OR gene OR based OR Allele * OR Genotyp * OR susceptibility). Selection criteria, data extraction and quality evaluation, and statistical analysis of articles were performed. For MnSOD, there were 2351 cases, including 1048 patients and 1303 control. For CAT, there were 2166 cases, including 987 patients and 1179 control. For GPX1, there were 1437 cases, including 614 patients and 823 control. The recessive model (Ala/Ala vs. Val/Val/Val + Ala) of MnSOD was associated with the risk of HCC (OR = 1.49,[95% CI: 1.04, 2.15]) (P = 0.03). MnSOD (allele model[Ala vs. Val], codominant model[Ala/Ala vs. Val/Val], codominant model[Val/Ala vs. Val/Val], and dominant model[Ala/Ala + Val/Ala vs. Val/Val]), CAT (262 C > T) (allele model[T vs. C], codominant model[TT vs. CC], codominant model[CT vs. CC], dominant model[TT + CT vs. CC], and recessive model[TT vs. CT + CT]), and GPX1 (Pro198Leu) (allele model[Leu vs. Pro], codominant model[Leu/Leu vs. Pro/Pro], codominant model[Leu/ Pro vs. Pro/Pro], dominant model[Leu/Leu + Leu/Pro vs. Pro/Pro] and recessive model[Leu/Leu vs. Pro/Pro + Leu/Pro]) were no significant correlation with the incidence of HCC (P > 0.05). The implicit model (Ala/Ala vs. Val/ Val/Val + Ala) of MnSOD was significantly correlated with the incidence of HCC. However, CAT (262 C > T) and GPX1 (Pro198Leu) were no significant correlation with the incidence of HCC. © 2016, E-Century Publishing Corporation. All rights reserved.


Weeda S.,Virginia State University | Zhang N.,China Agricultural University | Zhao X.,Tianjin Academy of Agricultural Science | Ndip G.,Virginia State University | And 4 more authors.
PLoS ONE | Year: 2014

Melatonin is a ubiquitous molecule and exists across kingdoms including plant species. Studies on melatonin in plants have mainly focused on its physiological influence on growth and development, and on its biosynthesis. Much less attention has been drawn to its affect on genome-wide gene expression. To comprehensively investigate the role(s) of melatonin at the genomics level, we utilized mRNA-seq technology to analyze Arabidopsis plants subjected to a 16-hour 100 pM (low) and 1 mM (high) melatonin treatment. The expression profiles were analyzed to identify differentially expressed genes. 100 pM melatonin treatment significantly affected the expression of only 81 genes with 51 down-regulated and 30 up-regulated. However, 1 mM melatonin significantly altered 1308 genes with 566 up-regulated and 742 down-regulated. Not all genes altered by low melatonin were affected by high melatonin, indicating different roles of melatonin in regulation of plant growth and development under low and high concentrations. Furthermore, a large number of genes altered by melatonin were involved in plant stress defense. Transcript levels for many stress receptors, kinases, and stress-associated calcium signals were up-regulated. The majority of transcription factors identified were also involved in plant stress defense. Additionally, most identified genes in ABA, ET, SA and JA pathways were up-regulated, while genes pertaining to auxin responses and signaling, peroxidases, and those associated with cell wall synthesis and modifications were mostly down-regulated. Our results indicate critical roles of melatonin in plant defense against various environmental stresses, and provide a framework for functional analysis of genes in melatonin-mediated signaling pathways. © 2014 Weeda et al.


Xiao H.-B.,China Agricultural University | Chen Q.,Tianjin Academy of Agricultural science | Wang J.-M.,Chinese Academy of Agricultural Sciences | Oxley L.,University of Waikato | Ma H.-Y.,Henan Agricultural University
Journal of Integrative Agriculture | Year: 2015

From 1985, an increasing gap has emerged between the official statistical measures of meat production and meat consumption in China, which has raised concerns from many researchers using such data. In this paper we report the results of 428 observations (survey of 107 urban and rural households×4 quarters) from 7 provinces conducted in 2010, and compare them with the official statistical data from the National Bureau of Statistics of China (NBSC). We conclude that the main reason for the discrepancy is due to the underreporting of consumption, which is due mainly to the omission of consumption away from home. © 2015 Chinese Academy of Agricultural Sciences.


Liu B.,Chinese Academy of Agricultural Sciences | Liu B.,Tianjin Academy of Agricultural science | Yan F.,Henan Agricultural University | Chu D.,Qingdao Agricultural University | And 8 more authors.
International Journal of Biological Sciences | Year: 2012

In China, Bemisia tabaci Q (commonly known as biotype Q) has rapidly displaced B (commonly known as biotype B) in the past 6 years. The mechanisms underlying such phenomenon have been studied extensively in recent years; however, we have not come to a definitive con-clusion yet. In the present study, the differences in host suitability between B and Q whitefly adults to five host plants (cabbage, cotton, cucumber, poinsettia, and tomato) were evaluated based on their respective feeding behaviors using a direct-current electrical penetration graph (DC-EPG) system. Pair-wise comparisons of B. tabaci B and Q feeding on each of the five host plants clearly indicate that Q feeds better than B on tomato, cotton and poinsettia, while B feeds better than Q on cabbage and cucumber. The EPG parameters related to both phloem and non-phloem phases confirm that cabbage and cucumber are best suited to B, while to-mato, cotton, and poinsettia are best suited to Q. Our present results support the contention that host suitability and adult feeding behavior contribute to the competitive displacement of biotype B by biotype Q. The discrepancy between field (previous studies) and laboratory results (this study), however, suggests that 1) whitefly displacement is apparently contributed by multiple factors; and 2) factor(s) other than the host plant suitability may play a vital role in dictating the whitefly biotypes in the field. © Ivyspring International Publisher.


Hu Y.-C.,TU Munich | Song Z.-W.,Tianjin Academy of Agricultural science | Lu W.-L.,Tianjin Academy of Agricultural science | Poschenrieder C.,TU Munich | Schmidhalter U.,TU Munich
Pedosphere | Year: 2012

Nine districts covering the main greenhouse vegetable areas in Tianjin Municipality of the North China Plain were selected for the soil investigation in 2010 to survey the current soil nutrient status (soil available N, P and K), acidification and salinization due to excessive input of fertilizers in greenhouses in Tianjin. The study showed that, in particular, soil available P content increased with the age of greenhouses. In contrast, our results did not reveal higher K accumulation and lowered pH in the greenhouse soils compared with cultivation in open fields. Over-fertilization, causing high NO3- accumulation, most likely resulted in salinity problems in the greenhouses. Ninety percent of the investigated greenhouse soils had electrical conductivity values of saturated paste extracts of 2-10 d S m-1, which might affect the yields of vegetable crops like green bean, pepper, cabbage, carrot, eggplant, lettuce, spinach, celery, cucumber and tomato. The findings of our survey of the current fertility and salinity problems in greenhouse soils suggest that there is an urgent need to improve the farmers' practices and strategies in fertilization management in greenhouses of China. Because education and the agricultural technical extension services may play a more important role in avoiding overuse of fertilizers, we suggest that current nutrient management practices should be improved in the near future through training of local farmers in farmers' schools and through strengthening the agricultural extension services with practical techniques. © 2012 Soil Science Society of China.


Yang L.,Nankai University | Han H.,Nankai University | Liu M.,Nankai University | Zuo Z.,Tianjin Academy of Agricultural science | And 5 more authors.
Plant Cell, Tissue and Organ Culture | Year: 2013

Salt stress has attracted increasing attention due to its toxic ability to restrict plant growth, and the photorespiration pathway has been shown to develop improved plant tolerance to abiotic stress. In this study, an Arabidopsis photorespiratory pathway gene serine: glyoxylate aminotransferase (SGAT), named as AtAGT1, was successfully overexpressed in duckweed (Lemna minor) to investigate the salinity defense capability in three transgenic overexpressed (OE) lines. Increased SGAT activity and decreased endogenous serine levels in these transgenic plant lines under salt stress resulted in enhanced protection against root abscission, higher maximum quantum yield of photosystem II (Fv/Fm), increased defense from cell damage as a result of improved cell membrane integrity, a decrease of reactive oxygen species (ROS) accumulation, and a strengthened antioxidant system. The salt tolerance in these transgenic OE lines indicates that the improvement of photorespiration stimulated the antioxidant system to scavenge ROS. The change of serine level also suggests the role of serine during salt stress. This transgenic engineering in duckweed not only introduced salt tolerance to this aquatic plant but also reveals a significant role of photorespiration during salinity stress. © 2013 Springer Science+Business Media Dordrecht.


Zhang Y.,Nankai University | Sun Y.,Tianjin Academy of Agricultural science | Zhang J.,Nankai University | Xu J.,Nankai University | Wu Y.,Nankai University
Concurrency Computation | Year: 2014

SUMMARYIn high-speed network monitoring, the ever-growing traffic calls for a high-performance solution for the computation of frequent items. The increasing number of cores in the current commodity multi-core processors opens up new opportunities in parallelization. In this paper, we present a novel precision integrated framework (PRIF) that exploits the great parallel capability of multi-cores to speed up the famous frequent algorithm. PRIF equally distributes the input data stream into sub-threads that use the optimized weighted frequent algorithm to track local frequent items. The items with frequency increments exceeding a pre-defined threshold are sent to a merging thread which is able to return the global continuous ε-deficient frequent items. The theoretical correctness and complexity analyses are presented. Experiments with real and synthetic traces confirm the theoretical analyses and demonstrate the excellent performance as well as the effects of parameters and data skewness. The results show that PRIF is able to provide continuous frequent items and near-linear speedup at the cost of greater memory use. Copyright © 2013 John Wiley & Sons, Ltd. Copyright © 2013 John Wiley & Sons, Ltd.


Chen Y.,Tianjin Academy of Agricultural Science | Xiao C.-P.,Nankai University | Chen X.-Y.,Tianjin Academy of Agricultural Science | Yang L.-W.,Tianjin Academy of Agricultural Science | And 4 more authors.
Journal of Molecular Catalysis B: Enzymatic | Year: 2014

In this work, a novel method to prepare cross-linked enzyme aggregates (CLEA) in a water-in-oil emulsion was reported. Spherical CLEA of recombinant trehalose synthase (TreS) from Meiothermus rubber was obtained through the emulsion based process. By varying the experimental parameters, the optimum conditions for the preparation of spherical CLEA were determined. The optimum catalytic temperature and thermal stability of spherical CLEA were enhanced significantly compared with the free enzyme. Spherical CLEA was also more tolerant to metal ions and chemical denaturants. Optical and scanning electron microscopy analysis showed that instead of the amorphous clumping shape of conventionally prepared CLEA, spherical CLEA has a more structured ball-like appearance with a size of 20-60 μm in diameter that could be easily separated. © 2013 Elsevier B.V.

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