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Zhang Y.,Nankai University | Sun Y.,Tianjin Academy of Agricultural science | Zhang J.,Nankai University | Xu J.,Nankai University | Wu Y.,Nankai University
Concurrency Computation

SUMMARYIn high-speed network monitoring, the ever-growing traffic calls for a high-performance solution for the computation of frequent items. The increasing number of cores in the current commodity multi-core processors opens up new opportunities in parallelization. In this paper, we present a novel precision integrated framework (PRIF) that exploits the great parallel capability of multi-cores to speed up the famous frequent algorithm. PRIF equally distributes the input data stream into sub-threads that use the optimized weighted frequent algorithm to track local frequent items. The items with frequency increments exceeding a pre-defined threshold are sent to a merging thread which is able to return the global continuous ε-deficient frequent items. The theoretical correctness and complexity analyses are presented. Experiments with real and synthetic traces confirm the theoretical analyses and demonstrate the excellent performance as well as the effects of parameters and data skewness. The results show that PRIF is able to provide continuous frequent items and near-linear speedup at the cost of greater memory use. Copyright © 2013 John Wiley & Sons, Ltd. Copyright © 2013 John Wiley & Sons, Ltd. Source

Yang L.,Nankai University | Han H.,Nankai University | Liu M.,Nankai University | Zuo Z.,Tianjin Academy of Agricultural science | And 5 more authors.
Plant Cell, Tissue and Organ Culture

Salt stress has attracted increasing attention due to its toxic ability to restrict plant growth, and the photorespiration pathway has been shown to develop improved plant tolerance to abiotic stress. In this study, an Arabidopsis photorespiratory pathway gene serine: glyoxylate aminotransferase (SGAT), named as AtAGT1, was successfully overexpressed in duckweed (Lemna minor) to investigate the salinity defense capability in three transgenic overexpressed (OE) lines. Increased SGAT activity and decreased endogenous serine levels in these transgenic plant lines under salt stress resulted in enhanced protection against root abscission, higher maximum quantum yield of photosystem II (Fv/Fm), increased defense from cell damage as a result of improved cell membrane integrity, a decrease of reactive oxygen species (ROS) accumulation, and a strengthened antioxidant system. The salt tolerance in these transgenic OE lines indicates that the improvement of photorespiration stimulated the antioxidant system to scavenge ROS. The change of serine level also suggests the role of serine during salt stress. This transgenic engineering in duckweed not only introduced salt tolerance to this aquatic plant but also reveals a significant role of photorespiration during salinity stress. © 2013 Springer Science+Business Media Dordrecht. Source

Liu M.,China Institute of Technology | Ning B.,China Institute of Technology | Qu L.,Huazhong Agricultural University | Peng Y.,China Institute of Technology | And 5 more authors.
Sensors and Actuators, B: Chemical

In this work, we demonstrate the development of a highly sensitive and selective surface plasmon resonance (SPR) immunosensor for label-free detection of ractopamine (RCT) as low as 0.12 ppb (ng mL -1) direct from pork liver samples. RCT is a kind of the β-adrenergic agonist with N-alkylphenyl substituent, and the development of high performance sensing methods for RCT is a critical issue because of its vital role in human metabolism. To construct the immunosensor system, the biosensor surface was formed by immobilization of ractopamine-ovalbumin (RCT-OVA) conjugate onto an Au-thiolate self-assembled SPR sensor chip by covalent amide binding, and the detection of RCT was performed based on indirect competitive inhibition principle. Detailed works were carried out focusing on optimizing the conditions that affect immunosensitivity, including immobilization buffer, immunobinding buffer, RCT-OVA concentration, antibody concentration, activation time, regeneration buffer, etc. A preferable way to determine the optimal antibody concentration was performed comparing with that used in most other reported researches. Under the optimal conditions, the biosensor exhibits a linear detection range of 0.28-4.29 ng mL -1, LOD of 0.12 ng mL -1 and IC 50 of 1.17 ng mL -1. It improved that the developed SPR immunosensor is more sensitive for detection of RCT against other reported ones. © 2011 Elsevier B.V. All rights reserved. Source

Xiao H.-B.,China Agricultural University | Chen Q.,Tianjin Academy of Agricultural science | Wang J.-M.,Chinese Academy of Agricultural Sciences | Oxley L.,University of Waikato | Ma H.-Y.,Henan Agricultural University
Journal of Integrative Agriculture

From 1985, an increasing gap has emerged between the official statistical measures of meat production and meat consumption in China, which has raised concerns from many researchers using such data. In this paper we report the results of 428 observations (survey of 107 urban and rural households×4 quarters) from 7 provinces conducted in 2010, and compare them with the official statistical data from the National Bureau of Statistics of China (NBSC). We conclude that the main reason for the discrepancy is due to the underreporting of consumption, which is due mainly to the omission of consumption away from home. © 2015 Chinese Academy of Agricultural Sciences. Source

Weeda S.,Virginia State University | Zhang N.,China Agricultural University | Zhao X.,Tianjin Academy of Agricultural science | Ndip G.,Virginia State University | And 4 more authors.

Melatonin is a ubiquitous molecule and exists across kingdoms including plant species. Studies on melatonin in plants have mainly focused on its physiological influence on growth and development, and on its biosynthesis. Much less attention has been drawn to its affect on genome-wide gene expression. To comprehensively investigate the role(s) of melatonin at the genomics level, we utilized mRNA-seq technology to analyze Arabidopsis plants subjected to a 16-hour 100 pM (low) and 1 mM (high) melatonin treatment. The expression profiles were analyzed to identify differentially expressed genes. 100 pM melatonin treatment significantly affected the expression of only 81 genes with 51 down-regulated and 30 up-regulated. However, 1 mM melatonin significantly altered 1308 genes with 566 up-regulated and 742 down-regulated. Not all genes altered by low melatonin were affected by high melatonin, indicating different roles of melatonin in regulation of plant growth and development under low and high concentrations. Furthermore, a large number of genes altered by melatonin were involved in plant stress defense. Transcript levels for many stress receptors, kinases, and stress-associated calcium signals were up-regulated. The majority of transcription factors identified were also involved in plant stress defense. Additionally, most identified genes in ABA, ET, SA and JA pathways were up-regulated, while genes pertaining to auxin responses and signaling, peroxidases, and those associated with cell wall synthesis and modifications were mostly down-regulated. Our results indicate critical roles of melatonin in plant defense against various environmental stresses, and provide a framework for functional analysis of genes in melatonin-mediated signaling pathways. © 2014 Weeda et al. Source

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