Wang J.,Tianjin 4th Center Hospital |
Cao W.,Tianjin Hospital |
Niu F.,Tianjin Hospital
Journal of Toxicological Sciences | Year: 2015
Insulin-like growth factor-1 (IGF-1), with an age-related decline, regulates the prolif-eration and survival of multiple cell types, particularly stimulates cartilage matrix synthesis, and inhib-its matrix degradation. The present study was to investigate the regulatory role of IGF-1 against hydrogen peroxide(H2O2)-induced mitochondrial dysfunction and apoptosis in murine chondrocytic ATDC5 cells. We firstly determined mitochondrial dysfunction and apoptosis in ATDC5 cells which were exposed to H2O2. We then constructed an IGF-1-overexpressed adenovirus (IGF-1-Ad) harboring the IGF-1 coding sequence, and investigated the regulatory role of the overexpressed IGF-1 against the H2O2-induced mitochondrial dysfunction and apoptosis in ATDC5 cells. It was demonstrated that H2O2 treatment promoted the mitochondrial dysfunction, and further reduced the viability and induced apoptosis of ATDC5 cells. However, the IGF-1 overexpression by adenovirus inhibited the H2O2-induced mitochondrial dysfunction and further inhibited the H2O2-promoted apoptosis in ATDC5 cells. In conclusion, the present study found that oxidative stress promoted mitochondrial dysfunction and induced apoptosis in the murine chondro-cytic ATDC5 cells, and the adenoviral vector-expressed IGF-1 protected the murine chondrocytic ATDC5 cells against such mitochondrial dysfunction and apoptosis. This study implies the protective role of IGF-1 against the oxidative stress in murine chondrocytic ATDC5 cells and demonstrates the promising anti-oxidative stress effect of the recombinant IGF-1-Ad against oxidative stress in chondrocytic cells. © 2015, Japanese Society of Toxicology. All rights reserved.
Wang Y.-R.,Tianjin 4th Center Hospital |
Yan J.-X.,Peoples Hospital of Wuqing |
Wang L.-N.,Second Peoples Hospital of Tianjin
Journal of Cancer Research and Therapeutics | Year: 2014
Objective: The aim of this study is to investigate whether or not serum carcino-embryonic antigen (CEA), alpha fetoprotein (AFP) and carbohydrate antigen 19-9 (CA19-9) can be used as potential biomarkers for colorectal cancer (CRC) diagnosis. Materials and Methods: Forty-six patients with pathology or cytology confirmed CRC and 36 cases with non-malignant colorectal disease (NMCD) were included in this retrospective study. The serum level of CEA, AFP and CA19-9 were arrayed and recorded for the two groups. The diagnosis sensitivity, specificity and area under the receiver-operating characteristic (ROC) curve for serum CEA, AFP and CA19-9 were calculated by the STATA-10.0 software. Results: We found that serum CEA, AFP and CA19-9 level in CRC patients were significantly higher than in NMCDs cases (all P < 0.05). The diagnosis sensitivity of CRC for CEA, AFP and CA19-9 were 80.43%, 73.91% and 69.57%; the diagnosis specificity of CRC for CEA, AFP and CA19-9 were 75.00%, 69.44% and 61.11%. The areas under the ROC curves for CEA, AFP and CA19-9, were 0.88, 0.78 and 0.77 respectively. Conclusion: These findings suggest that serum CEA, AFP and CA19-9 may be a useful biomarker for diagnosis of colorectal carcinoma against NMCD. © 2014, Medical Knowledge. All rights reserved.
Wang J.,Tianjin 4th Center Hospital |
Liu P.,Tianjin 4th Center Hospital
Journal of the Pakistan Medical Association | Year: 2015
Objectives: To evaluate and compare the long-term outcome of unstable thoracolumbar burst fractures treated using anterior, posterior or combined anterior and posterior approaches. Methods: The prospective randomised controlled study was conducted at the Tianjin 4th Centre Hospital, Tianjin, China, and comprised patients of unstable thoracolumbar burst fracture operated between July 2004 and July 2006 and followed up for five years. The patients were divided randomly into three groups of anterior, posterior and combined anterior and posterior approaches. Clinical data was analysed using SPSS 17. Results: Of the 66 cases in the study, 45(68.18%) were male and 21(31.8%) were female, with overall age ranging from 19 to 69 years. There were 22(33.3%) patients in the anterior group, 23(35%) in the posterior group, and 21(32%) in the combined anterior and posterior group. Comparison was made between two respective groups. The combined group was found to have the longest operation time (p<0.02; p<0.01, respectively), larger blood loss (p<0.006; p<0.005, respectively), longer hospital stay (p<0.01; p<0.003, respectively) and higher hospitalisation costs (p<0.004; p<0.001, respectively). The postoperative kyphotic angle was significantly smaller than preoperative one in all groups (p<0.01; p<0.02; p<0.01). Conclusion: The anterior approach or combined anterior and posterior approach were better options in managing unstable thoracolumbar burst fracture, while the latter should be used only for the burst fracture with a significant posterior column injury. © Pakistan Medical Association. All rights reserved.
Liu Y.,Tianjin 4th Center Hospital
Pakistan Journal of Medical Sciences | Year: 2012
Objective: To investigate the incidence of vascular injury in lumbar spine surgery and determine the strategy for its diagnosis and treatment. Methodology: We retrospectively reviewed 1159 patients in our institution treated with lumbar spine surgery from September 2003 to November 2009. Primary outcomes measured included intraoperative vascular complications, operative blood loss and operative mortality. Results: The incidence of vascular injury is significantly higher in anterior exposure (9.1%) than posterior exposure (0.29%) (p < 0.05) and 84.4% of vascular injury involved in L4-5 segment. Nineteen patients experienced vascular injury in two-level or multi-level exposure and 26 in one-level exposure, no significant difference between two groups was found (p > 0.05). One patient died and others got favourable results. Conclusion: L4-5 segment has high incidence of vascular injury especially when anterior exposure is performed, and spine surgeons should pay more attention to the fatal complication.
Shi L.,Tianjin 4th Center Hospital
Pakistan Journal of Medical Sciences | Year: 2013
Objective: To analyse the early outcome of main arterial injuries with delayed treatment in extremity trauma and help vascular surgeons in determining proper treatment strategy for such injuries. Methodology: Forty-three patients with delayed treatment of main arterial injuries during May 2003 and January 2008 were reviewed retrospectively. Results: In 43 patients, injuries were caused by blunt trauma in 26 cases and penetrating trauma in 17 cases. The maximum ischaemia time was 38 hours and the minimum was 13 hours. Eight patients underwent primary amputations and four patients underwent secondary amputation. There was no perioperative mortality, while wound infection occurred in five patients, followed by graft occlusion in four patients, arteriovenous fistulae in two patients and pseudoaneurysm in one patient. Conclusion: The delayed intervention in main arterial injuries is associated with higher risk of amputation, while the suitable surgical techniques may decrease the risk of limb loss. Viable limbs should be revascularized in otherwise stable patients even with long periods of ischaemia.
Liu S.,Tianjin 4th Center Hospital
Pakistan Journal of Medical Sciences | Year: 2014
Objective: To evaluate the clinical outcome of emergency and elective operation of splenectomy with periesophagogastric devascularization in treating upper gastrointestinal hemorrhage resulted from portal hypertension. Methods: We retrospectively reviewed 219 patients of upper gastrointestinal hemorrhage resulted from portal hypertension treated using emergency or elective operation between Jul 2002 and Aug 2010. The clinical data were collected and analyzed. Results: In the group of elective operation, four patients with grade B and three with grade C died, and in the group of emergency operation, two patients with Grade B and four with Grade C died. The Grade C patients treated using emergency operation presented with a higher mortality than those treated using elective operation, but no significant difference was found (p > 0.05). In the two groups, no patients with Grade A died. 17 cases (11.1%) suffered from complications in the group of elective operation and 11 cases (16.7%) in emergency operation (p > 0.05). The complication rate in patients with Grade C is significantly higher than that in patients with Grade A or B in each group (p < 0.05). The hospital stay and cost in group of elective operation are significantly higher than those in group of emergency operation (p < 0.05). Conclusion: The patients with Grade A or B treated using emergency operation have similar clinical outcomes as those treated using elective operation, but emergency operation may result in higher rate of death and complication in patients with Grade C.
Wang L.,Tianjin 4th Center Hospital
Pakistan Journal of Medical Sciences | Year: 2014
Objective: To evaluate the characterization of normal prevertebral soft tissue width of cervicothoracic region using magnetic resonance images. Methods: Prevertebral soft tissue width in the cervicothoracic region of 165 patients was measured using magnetic resonance images (MRI). The mean and standard deviation of these measurements were calculated and Spearman's correlation analysis was conducted to study the influences of age, sex and vertebral level on prevertebral soft tissue width. Results: The prevertebral soft tissue width in 165 subjects were measured and documented, and the value at each level is significantly different from those at adjacent level (p < 0.05). In addition, the width of prevertebral soft tissue in female and younger is significantly smaller than those in male and elders (p > 0.05). Conclusion: Vertebral level, sex and age have significant influence on prevertebral soft tissue width.
Zhang T.,Tianjin 4th Center Hospital |
Liu C.,Tianjin 4th Center Hospital
Pakistan Journal of Medical Sciences | Year: 2016
Objective: To determine whether continuing midwifery care has more benefits than standard maternity care in vaginal birth after cesarean (VBAC). Methods: This study was conducted on women in labour who had history of previous cesarean section and received vaginal birth in obstetrical department of our hospital from May 2013 to November 2014. The included patients were divided randomly into observation group and control group. The women in labour allocated to the observation group received continuing midwifery care, and those to control group received standard maternity care in all the stages of labour. The duration of labor stage together with the rate of fetal distress, neonatal asphyxia, vaginal birth and postpartum bleeding were compared between the two groups. Results: Ninety-six participants were included in the current study, forty-eight in each group. The length of labor was significantly longer (p<0.05), the vaginal birth rate was significantly lower (p<0.05) and the postpartum hemorrhage rate was significantly higher (p<0.05) in the control group than the observation group. In addition, the rate of fetal distress and neonatal asphyxia were higher in the control group, but there was no significant difference between the two groups (p>0.05). Conclusion: The continuing midwifery care has more benefits than the standard maternity care in vaginal birth after cesarean (VBAC). © 2016, Professional Medical Publications. All Rights Reserved.
Wang F.,Tianjin 4th Center Hospital
Pakistan Journal of Medical Sciences | Year: 2013
Objective: To analyze our experiences in patients with traumatic retroperitoneal hematoma, and highlight the problems in diagnosis and treatment to facilitate the surgeons to make decision. Methodology: One hundred and eight patients of traumatic retroperitoneal hematoma treated in our institution from May 2008 to Jun 2012 were reviewed retrospectively. The data including patient's age, type of injury, hospital stays, type of treatment, injured organs and mortality rate were collected. Results: In 108 patients, seventy-seven patients were male and 31 were female with a mean age of 36.5 years; eighty-seven patients sustained blunt trauma and 21 penetrating injury. Centro-medial hematoma was found in 31 patients, lateral hematoma in 36 patients, pelvic hematoma in 35 and pelvic-central hematoma in six patients. Eighty-three patients were treated surgically and 25 patients were treated conservatively. Six patients died and the mortality rate is 6.5%. Wound infection occurred in five patients, deep vein thrombosis in one patient, gastric fistula in two patients and duodenal fistula in one patient. Conclusion: Traumatic retroperitoneal hematoma is life-threatening condition, early diagnosis and correct treatment is of upmost importance. Mandatory exploration should be performed in cases of retroperitoneal hematomas resulting from penetrating injury, but the selection of treatment mode in blunt injury depends on the anatomical position of hematoma, visceral injury and the hemodynamic status of the patients.
Zhan Y.,Tianjin 4th Center Hospital |
Tian D.,Tianjin 4th Center Hospital
Turkish Neurosurgery | Year: 2012
AIM: To determine whether translaminar facet screws can provide stability equivalent to pedicle screws in two-level anterior lumbar interbody fusion. MATERIAL and METHODS: We performed a biomechanical study using 12 fresh human lumbar spines and tested intact spine, stand-alone two-level ALIF and anterior fusion augmented with pedicle screws or translaminar facet screws under 400N compressive preloads and 7.5Nm moments in flexion, extension, axial rotation and lateral bending, and measured the range of motion and the stiffness of operative level. RESULTS: We found two-level ALIF had significantly reduced range of motion, greater stiffness at operative segments in flexion, axial rotation and lateral bending (p<0.05), but decreased stiffness and increased range of motion in extension (p<0.05), when compared to intact spine. The two augmented ALIF constructs have significantly reduced range of motion (p<0.05) and increased stiffness at operative level (p<0.05), when compared to intact spine or stand-alone two-level ALIF. There is no significant difference of range of motion and stiffness at operative level between the two augmented constructs in all loading directions (p>0.05). CONCLUSION: Translaminar facet screws can provide stability equivalent to pedicle screws, and we recommend translaminar facet screws as perfect alternatives to pedicle screws in two-level ALIF.