Yu Z.-H.,Tianjin Medical University |
Yu Z.-H.,Tianin Key Laboratory of Cancer Prevention and Therapy |
Yu Y.,Tianjin Medical University |
Yu Y.,Tianin Key Laboratory of Cancer Prevention and Therapy |
And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: With the development of genetic engineering and tumor molecular biology, gene therapy for tumors has become a new treatment modality. OBJECTIVE: To explore the effect of the KDR gene silencing on the proliferation and invasion capacity of breast cancer MCF-7 cells. METHODS: Interfering RNA (siRNA) sequences for small molecule KDR gene was designed and transferred into human breast cancer MCF-7 cells. Then, RT-PCR and western blot assay were used to detect the KDR mRNA and protein expression. Flow cytometry, cell counting kit-8 test and Transwell test were employed to detect the cell cycle, proliferative capacity and invasion capacity of breast cancer MCF-7 cells after the KDR gene silencing. RESULTS AND CONCLUSION: After 48 hours of KDR silencing, the mRNA and protein expressions of KDR in MCF-7 cells were decreased obviously; MCF-7 cells arrested at G0/G1 stage and the number of cells at S stage was reduced. Cell proliferation was inhibited significantly. The amount of cells passing through the filtering membrane became less. After KDR gene silencing, the proliferation and invasion of breast cancer MCF-7 cancer stem cells were inhibited remarkably, indicating that KDR gene silencing may be a new target for the effective treatment of breast cancer. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research, All Right Reserved.