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Kepenekci I.,Plant Protection Central Research Institute | Tulek A.,Thrace Agricultural Research Institute | Alkan M.,Plant Protection Central Research Institute | Hazir S.,Adnan Menderes University
Pakistan Journal of Zoology | Year: 2013

The biocontrol efficiency of three Turkish isolates of the entomopathogenic nematodes Steinernema carpocapsae, S. feltiae and Heterorhabditis bacteriophora were evaluated against the last instar of potato tuber moth (PTM) Phthorimaea operculella under laboratory conditions. To determine optimum nematode application rate and temperature, the experiments were conducted with 100, 500 and 1000 IJs at 10, 15 and 25oC. Temperature and nematode concentration had a significant effect on P. operculella larval mortality. S. carpocapsae and H. bacteriophora species displayed generally increased virulence in parallel with rising temperature and the number of infective juveniles applied. At 25°C and 1000 IJs concentration, the larval mortality was 96 and 80% for S. carpocapsae and H. bacteriophora, respectively. However, S. feltiae did not exhibit more than 40% mortality at any temperature or concentration, except when the nematodes were applied in infected insect host cadavers. At 25°C, infected cadaver applications showed 97, 83 and 67% mortality for S. carpocapsae, H. bacteriophora and S. feltiae, respectively. Our results indicate that P. operculella larvae are quite susceptible to entomopathogenic nematode infection and, in particular, S. carpocapsae blacksea strain has a high level of potential to control this pest. Copyright 2013 Zoological Society of Pakistan.

Tulek A.,Thrace Agricultural Research Institute | Erturk S.,Plant Protection Central Research Institute | Kepenekci I.,Gaziosmanpasa University | Atay T.,Gaziosmanpasa University
Egyptian Journal of Biological Pest Control | Year: 2015

The three native entomopathogenic nematode (EPN) species; Steinernema feltiae, S. carpocapsae and Heterorhabditis bacteriophora, isolated from Turkey, were tested for the control of the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) under laboratory conditions. The study was conducted at the Department of Entomology, Plant Protection Central Research Institute, Ankara, Turkey in 2013. Suspensions of the nematodes were applied at three different concentrations (500, 1000 and 2000IJs insect-1 or approximately 78, 157 and 314IJcm-2) and incubated at three different temperatures (15, 20 and 25°C). Mortality rate of the beetle’s adults was assessed one week post application. Mortality rates were significantly higher at all the nematode treatments than in the control at 20 and 25°C. S. carpocapsae species showed highest mortality rates (37.95 and 54.06%) at the higher doses of (1000 and 2000IJs insect-1), respectively at 25°C. © 2016, Egyptian Society for Biological Control of Pests. All rights reserved.

Kepenekci I.,Gaziosmanpasa University | Oksal E.,Directorate of Apricot Research Station | Saglam H.D.,Ahi Evran University | Atay T.,Gaziosmanpasa University | And 2 more authors.
Egyptian Journal of Biological Pest Control | Year: 2015

Potato (Solanum tuberosum L.) is one of the most important vegetable crops in Turkey as well in the world. Potato plants are attacked by a large number of insect pests such as aphids, beetles, leafhoppers and lepidopterous pests under both field and storage conditions. The Colorado potato beetle (CPB) [Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)] and the potato tuber moth (PTM) [Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)] are the most destructive pests in potatoes grown areas. Many pesticides that used to control the potato pests are very toxic to environment and human. An alternative approach to the commercial pesticides is using biological agents like fungi. Purpureocillium lilacinum was described more than a century ago and is a commonly occurring fungus in soil. P. lilacinum (syn: Paecilomyces lilacinus) is the most widely tested fungus for the control of nematodes, aphids and insects. The fungus was determined in Turkey but its molecular identification has not been done yet. In this study, a Turkish isolate of P. lilacinum was identified by classical (morphologic and morphometric properties) and molecular methods. Also, the study concerned with the effect of different conidial concentrations of the fungal isolate on mortality rates of CPB and PTM at different temperatures. P. lilacinumwas found to be most effective on the last larval instar of CPB and PTM (33.2 and 43.3% mortality, respectively) on 10th day of treatment with the fungal concentration of 108cfu ml-1at 25°C. This fungus developed on all dead larvae and was highly susceptible at the tested temperatures, but its activity decreased at 15°C. Findings of this study showed a potential of the fungus as a bioagent against the CPB and the PTM. © 2016, Egyptian Society for Biological Control of Pests. All rights reserved.

Trials were conducted on the Thrace Agricultural Research Institute, Turkey in 2013. Three rice cultivars grown in Turkey (Halilbey and the advanced lines, Osmancik/Rus and Kral/Diana from the Turkish national breeding program) and two resistant cultivars from the International Rice Research Institute (Asahi and Rinaldo Bersani) were used to determine the effects of rice white-tip nematode, Aphelenchoides besseyi, on yield and yield components of rice. Infested plots that were created with a combination of infected seeds and supplemental inoculation with A. besseyi at the seedling stage and uninfested plots were compared. Yield was significantly reduced (P < 0.01) in nematode-infested plots in comparison with the control. The cultivars Halilbey, Rinaldo Bersani, Osmancik/Rus, Kral/Diana, and Asahi exhibited 36.2%, 18.1%, 19.3%, 28.2%, and 7.9% yield reduction, respectively. Nematode infection also reduced other agronomic traits including paddy and milled rice 1,000-kernel weight by 4.5% and 6.6%, respectively, and milled rice yield by 7.1%.

Comertpay G.,Eastern Mediterranean Agricultural Research Institute | Baloch F.S.,Abant Izzet Baysal University | Derya M.,Cukurova University | Andeden E.E.,Cukurova University | And 3 more authors.
Genetics and Molecular Research | Year: 2016

Effective breeding programs based on genetic diversity are needed to broaden the genetic basis of rice (Oryza sativa L.) in Turkey. In this study, 81 commercial varieties from seven countries were studied in order to estimate the genomic relationships among them using nine inter-primer binding site (iPBS)-retrotransposon and 17 simple-sequence repeat (SSR) markers. A total of 59 alleles for the SSR markers and 96 bands for the iPBS-retrotransposon markers were detected, with an average of 3.47 and 10.6 per locus, respectively. Each of the varieties could be unequivocally identified by the SSR and iPBS-retrotransposon profiles. The iPBS-retrotransposon- and SSR-based clustering were identical and closely mirrored each other, with a significantly high correlation (r = 0.73). A neighbor-joining cluster based on the combined SSR and iPBSretrotransposon data divided the rice varieties into three clusters. The population structure was determined using the STRUCTURE software, and three populations (K = 3) were identified among the varieties studied, showing that the diversity harbored by Turkish rice varieties is low. The results indicate that iPBS-retrotransposon markers are a very powerful technique to determine the genetic diversity of rice varieties. © FUNPEC-RP.

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