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Bilgin O.,Namik Kemal University | Korkut K.Z.,Namik Kemal University | Baser I.,Namik Kemal University | Daglioglu O.,Namik Kemal University | And 3 more authors.
Pakistan Journal of Botany | Year: 2011

In this study which was performed with 20 durum wheat genotypes at three locations during two crop seasons. The relative large variations were observed for the characters except test weight and days to 50% flowering. The highest heritability and expected genetic advance values were estimated in plant height (92.8%-9.84) and 1000 grain weight (90.2%-5.96). The lowest value of heritability and expected genetic advance were obtained for grain yield (51.4%-0.94) and test weight (63.8%-2.41). Grain yield was positive and significantly correlated with grain weight/spike, test weight, plant height, grains/spike, spikelets/spike, 1000 grain weight and spike length whereas it showed negative and important correlation coefficient with days to 50% flowering. The highest direct positive effect (0.3857) on yield was computed for grain weight/spike. On the other hand, days to 50% flowering and spike length had negative direct effect on yield. Although grain weight/spike should be emphasised as reliable selection criteria, grains/spike, 1000 grain weight, plant height and earliness might be considered as indirect selection criterion for improvement in durum wheat yield potential.


Tulek A.,Thrace Agricultural Research Institute | Erturk S.,Plant Protection Central Research Institute | Kepenekci I.,Gaziosmanpaşa University | Atay T.,Gaziosmanpaşa University
Egyptian Journal of Biological Pest Control | Year: 2015

The three native entomopathogenic nematode (EPN) species; Steinernema feltiae, S. carpocapsae and Heterorhabditis bacteriophora, isolated from Turkey, were tested for the control of the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) under laboratory conditions. The study was conducted at the Department of Entomology, Plant Protection Central Research Institute, Ankara, Turkey in 2013. Suspensions of the nematodes were applied at three different concentrations (500, 1000 and 2000IJs insect-1 or approximately 78, 157 and 314IJcm-2) and incubated at three different temperatures (15, 20 and 25°C). Mortality rate of the beetle’s adults was assessed one week post application. Mortality rates were significantly higher at all the nematode treatments than in the control at 20 and 25°C. S. carpocapsae species showed highest mortality rates (37.95 and 54.06%) at the higher doses of (1000 and 2000IJs insect-1), respectively at 25°C. © 2016, Egyptian Society for Biological Control of Pests. All rights reserved.


Kepenekci I.,Gaziosmanpaşa University | Oksal E.,Directorate of Apricot Research Station | Saglam H.D.,Ahi Evran University | Atay T.,Gaziosmanpaşa University | And 2 more authors.
Egyptian Journal of Biological Pest Control | Year: 2015

Potato (Solanum tuberosum L.) is one of the most important vegetable crops in Turkey as well in the world. Potato plants are attacked by a large number of insect pests such as aphids, beetles, leafhoppers and lepidopterous pests under both field and storage conditions. The Colorado potato beetle (CPB) [Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)] and the potato tuber moth (PTM) [Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)] are the most destructive pests in potatoes grown areas. Many pesticides that used to control the potato pests are very toxic to environment and human. An alternative approach to the commercial pesticides is using biological agents like fungi. Purpureocillium lilacinum was described more than a century ago and is a commonly occurring fungus in soil. P. lilacinum (syn: Paecilomyces lilacinus) is the most widely tested fungus for the control of nematodes, aphids and insects. The fungus was determined in Turkey but its molecular identification has not been done yet. In this study, a Turkish isolate of P. lilacinum was identified by classical (morphologic and morphometric properties) and molecular methods. Also, the study concerned with the effect of different conidial concentrations of the fungal isolate on mortality rates of CPB and PTM at different temperatures. P. lilacinumwas found to be most effective on the last larval instar of CPB and PTM (33.2 and 43.3% mortality, respectively) on 10th day of treatment with the fungal concentration of 108cfu ml-1at 25°C. This fungus developed on all dead larvae and was highly susceptible at the tested temperatures, but its activity decreased at 15°C. Findings of this study showed a potential of the fungus as a bioagent against the CPB and the PTM. © 2016, Egyptian Society for Biological Control of Pests. All rights reserved.


Trials were conducted on the Thrace Agricultural Research Institute, Turkey in 2013. Three rice cultivars grown in Turkey (Halilbey and the advanced lines, Osmancik/Rus and Kral/Diana from the Turkish national breeding program) and two resistant cultivars from the International Rice Research Institute (Asahi and Rinaldo Bersani) were used to determine the effects of rice white-tip nematode, Aphelenchoides besseyi, on yield and yield components of rice. Infested plots that were created with a combination of infected seeds and supplemental inoculation with A. besseyi at the seedling stage and uninfested plots were compared. Yield was significantly reduced (P < 0.01) in nematode-infested plots in comparison with the control. The cultivars Halilbey, Rinaldo Bersani, Osmancik/Rus, Kral/Diana, and Asahi exhibited 36.2%, 18.1%, 19.3%, 28.2%, and 7.9% yield reduction, respectively. Nematode infection also reduced other agronomic traits including paddy and milled rice 1,000-kernel weight by 4.5% and 6.6%, respectively, and milled rice yield by 7.1%.


Kepenekci I.,Plant Protection Central Research Institute | Tulek A.,Thrace Agricultural Research Institute | Alkan M.,Plant Protection Central Research Institute | Hazir S.,Adnan Menderes University
Pakistan Journal of Zoology | Year: 2013

The biocontrol efficiency of three Turkish isolates of the entomopathogenic nematodes Steinernema carpocapsae, S. feltiae and Heterorhabditis bacteriophora were evaluated against the last instar of potato tuber moth (PTM) Phthorimaea operculella under laboratory conditions. To determine optimum nematode application rate and temperature, the experiments were conducted with 100, 500 and 1000 IJs at 10, 15 and 25oC. Temperature and nematode concentration had a significant effect on P. operculella larval mortality. S. carpocapsae and H. bacteriophora species displayed generally increased virulence in parallel with rising temperature and the number of infective juveniles applied. At 25°C and 1000 IJs concentration, the larval mortality was 96 and 80% for S. carpocapsae and H. bacteriophora, respectively. However, S. feltiae did not exhibit more than 40% mortality at any temperature or concentration, except when the nematodes were applied in infected insect host cadavers. At 25°C, infected cadaver applications showed 97, 83 and 67% mortality for S. carpocapsae, H. bacteriophora and S. feltiae, respectively. Our results indicate that P. operculella larvae are quite susceptible to entomopathogenic nematode infection and, in particular, S. carpocapsae blacksea strain has a high level of potential to control this pest. Copyright 2013 Zoological Society of Pakistan.


Onemli F.,Namik Kemal University | Gucer T.,Thrace Agricultural Research Institute
Helia | Year: 2010

This present investigation was undertaken to determine some plant characters In six wild Helianthus genotypes and their test hybrids in 2007 and 2008. Helianthus petiolarls spp. petlolaris (E-142), Helianthus neglectus (E-017) and four wild Helianthus annuus (E-060, E-173, E-174 and E-175) were used as the material. The morphological characters observed in the wild Helianthus genotypes were plant height, head diameter, lateral number, number of days to 50% flowering, flowering period length, and presence of anthocyanin in the cotyledons. In the second year, test hybrids of E-017, E-142, E-173 and E-175 with the female Une 2453-A were evaluated for seed yield, seed weight and some morphological traits. The results showed that plant height, head diameter and lateral number for these wild genotypes ranged from 63 to 171 cm, 2.4 to 8 cm and 43 to 324.3, respectively. The wild sunflower genotypes needed 82-105 days after emergence to reach 50% flowering and had flowering periods of 67-91 days. E-142 (Helianthus petlolarls spp. petiolaris) and its test hybrid with the female line 2453A were similarly in the early group for days to 50% flowering. E-017 (Helianthus neglectus) and its test hybrids were similarly late for days to 50% flowering. Interestingly, although E-173 had medium plant height, its test hybrid had the shortest plants.


Onemli F.,Namik Kemal University | Gucer T.,Thrace Agricultural Research Institute
Helia | Year: 2010

Response of six wild sunflower genotypes including Helianthus petiolaris spp. petiolaris (E-142), Helianthus neglectus (E-017) and Helianthus annuus (E-060, E-173, E-174 and E-175) to drought stress imposed at the seedling growth stage was investigated in vivo. Plant height, number of leaves per plant, shoot fresh weight, root fresh weight, shoot dry weight and root dry weight were determined. Results indicated that the E-175 genotype belonging to Helianthus annuus was less affected by water stress conditions as compared to the other genotypes. Helianthus petiolaris spp. petiolaris (E-142) showed the highest sensitivity and had the lowest fresh and dry masses under drought conditions. In addition, this study showed that the number of leaves and root weight were the best selection criteria to determine drought resistance at the early vegetative stage. Water losses of the resistant genotypes In their roots and shoots in drought stress conditions were more than those of the sensitive genotypes.


Onemli F.,Namik Kemal University | Gucer T.,Thrace Agricultural Research Institute
Helia | Year: 2010

The objectives of this study were to determine self-fertility and restoration capability of cytoplasmic male fertility of some wild sunflower genotypes. The evaluated genotypes were Helianthus petlolarls spp. petlolarls (E-142), Helianthus neglectus (E-017) and Helianthus annuus (E-060, E-173, E-174 and E-175). For hybridization, the female inbred line 2453-A was used. Results showed that self fertility ratios of wild sunflower genotypes varied between 22.4 and 66.1%. The highest level of self fertility was observed in Helianthus petlolarls spp. petlolarls (E-142), while Helianthus neglectus had the lowest values. The E-060 and E-174 genotypes did not restore cytoplasmic male sterility in the hybrids tested.


Yildirim K.,Gaziosmanpaşa University | Yildirim K.,Middle East Technical University | Boylu B.,Middle East Technical University | Atici E.,Middle East Technical University | And 2 more authors.
Journal of Plant Diseases and Protection | Year: 2012

Rust and powdery mildew diseases of wheat are among the oldest and most harmful diseases confining the yield of wheat worldwide. Development of resistant wheat cultivars with durable or race-nonspecific resistance genes is the main objective for many breeding programs. One of these genes, Lr34/Yr18/Pm38, was found to confer partial and durable resistance against rust pathogens as well as powdery mildew. This multiple disease resistant locus was recently found to comprise a single gene, which encodes an adenosine triphosphate-binding cassette transporter (ABCtransporter) of the pleiotropic drug resistance (PDR) subfamily. Differences in the alleles of resistant and susceptible cultivars enabled to develop six allele-specific markers. In this study, 46 different Turkish bread wheat cultivars were screened using these specific markers, which indicated that 13 cultivars carry the resistance allele of that gene. To compare this molecular finding with phenotypic observations, a field trial with artificial inoculation of Puccinia recondita was performed. Interestingly, all cultivars having the resistance allele were found to be susceptible. Although Lr34 has mediated resistance against leaf rust for over 40 years and has been used globally as an effective component of rust resistance in many breeding programs, this adult plant resistance is ineffective in the studied Turkish bread wheat cultivars. © Eugen Ulmer KG, Stuttgart.


PubMed | Thrace Agricultural Research Institute, Eastern Mediterranean Agricultural Research Institute, Cukurova University and Abant Izzet Baysal University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Effective breeding programs based on genetic diversity are needed to broaden the genetic basis of rice (Oryza sativa L.) in Turkey. In this study, 81 commercial varieties from seven countries were studied in order to estimate the genomic relationships among them using nine inter-primer binding site (iPBS)-retrotransposon and 17 simple-sequence repeat (SSR) markers. A total of 59 alleles for the SSR markers and 96 bands for the iPBS-retrotransposon markers were detected, with an average of 3.47 and 10.6 per locus, respectively. Each of the varieties could be unequivocally identified by the SSR and iPBS-retrotransposon profiles. The iPBS-retrotransposon- and SSR-based clustering were identical and closely mirrored each other, with a significantly high correlation (r = 0.73). A neighbor-joining cluster based on the combined SSR and iPBS-retrotransposon data divided the rice varieties into three clusters. The population structure was determined using the STRUCTURE software, and three populations (K = 3) were identified among the varieties studied, showing that the diversity harbored by Turkish rice varieties is low. The results indicate that iPBS-retrotransposon markers are a very powerful technique to determine the genetic diversity of rice varieties.

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