The Third Peoples Hospital Of Yunnan Province

Kunming, China

The Third Peoples Hospital Of Yunnan Province

Kunming, China
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Zheng S.,The Third Peoples Hospital Of Yunnan Province | Yang J.,The Third Peoples Hospital Of Yunnan Province | Tang Y.-M.,Kunming Medical University
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Studies have shown that the main functions of mesenchymal stem cells include direct participation in wound healing, growth factor secretion, promoting angiogenesis, immune regulation and inflammation, anti-oxidative stress, which can be used to treat a variety of acute and chronic diseases. OBJECTIVE: To review advances in mesenchymal stem cells in the inflammatory immunomodulation. METHODS: A computer-based search of Wanfang, CNKI and PubMed databases was performed for articles concerning advances in mesenchymal stem cells in the inflammatory immunomodulation published from January 2005 to August 2015. The search terms were “stem cells, mesenchymal stem cells, immune regulation, inflammation, immune cells, inflammatory factors, treatment” in Chinese and English, respectively. Finally, 40 articles were included in result analysis. RESULTS AND CONCLUSION: Because of their immunomodulation and muti-directional differentiation, mesenchymal stem cells garner increasing attentions. In addition, mesenchymal stem cells can be harvested from different tissues and have good in vitro amplification capability, which have a broad prospect in the clinical use, including tissue repair and anti-inflammation. As the most promising cells used clinically, mesenchymal stem cells show their superiority in the treatment of many diseases, especially in inflammations induced by immune modulation imbalance. We believe that mesenchymal stem cells will play an important role in the future cell biotherapy. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Zhou L.,The Third Peoples Hospital Of Yunnan Province | Yang H.-L.,The Third Peoples Hospital Of Yunnan Province | Cao X.-J.,Nanjing Medical University
Chinese Journal of Tissue Engineering Research | Year: 2016

BACKGROUND: Due to complicated and special lumbar spinal stenosis disease and different degrees of lumbar degeneration in the elderly, it requires very careful choice of repair methods and repair technology. OBJECTIVE: To investigate the indications and prognosis effect of the treatments of elderly lumbar spinal stenosis by selective decompression of lumbar root canal under direct vision and laminectomy. METHODS: Clinical data of 167 elderly patients with lumbar spinal stenosis were retrospectively analyzed. A total of 82 patients were treated with selective decompression of lumbar root canal as the selective decompression group, with a clear nerve root compression but without central canal stenosis. A total of 85 patients were treated with laminectom and treated with pedicle screw fixation as the whole lamina cut group, with distinct central canal stenosis. The indications were analyzed between the two groups. Clinical effects were assessed using Japanese Orthopaedic Association and Oswestry Disability Index before and after surgery. RESULTS AND CONCLUSION: (1) All patients were operated successfully. There was no serious complication during treatment. (2) Japanese Orthopaedic Association and Oswestry Disability Index were significantly improved at last follow-up in two groups compared with preoperation (P < 0.05). No significant difference in Japanese Orthopaedic Association and Oswestry Disability Index was detected between the two groups at various time points (P > 0.05). Clinical symptoms disappeared or relieved at last follow-up in two groups. The prognosis in two groups had significantly improved. (3) Results indicated that laminectomy and pedicle screw fixation in elderly patients with lumbar spinal stenosis and central canal stenosis obtained significant effects. For elderly patients with lumbar spinal stenosis but without central canal stenosis, selective decompression of lumbar root canal under direct vision was given, and it is effective and reliable. The two methods are suitable for different patients, and are effective and reliable way to repair. © 2016, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


PubMed | The Peoples Hospital of Wenshan Prefecture, Sun Yat Sen University, Kunming Medical University and the Third Peoples Hospital of Yunnan Province
Type: Journal Article | Journal: Journal of the renin-angiotensin-aldosterone system : JRAAS | Year: 2015

The renin-angiotensin-aldosterone system is important to the development of atrial fibrillation (AF). A lot of research has focused on the relationship between angiotensin-converting enzyme (ACE) insertion (I) /deletion (D) gene polymorphisms and AF, with inconsistent results. A meta-analysis was carried out to find the correlation between ACE I/D gene polymorphisms and AF.Data were extracted from articles published before September 2013 on ACE I/D polymorphisms and AF in Embase, PubMed, WanFangData, and China National Knowledge Infrastructure.The recessive model found that ACE I/D gene polymorphisms were related to AF (odds ratio (OR) = 1.61, 95% confidence interval (CI) = 1.16-1.72). Subgroup analysis showed a significant association in the recessive model for Asian (OR = 1.40, 95% CI = 1.19-1.80) and Caucasian (OR = 1.42, 95% CI = 1.01-1.99) populations.ACE I/D gene polymorphisms and AF are significantly related to ethnicity. Individuals with the ACE D/D genotype appear to be at higher risk of AF.


Yin Y.,The Third Peoples Hospital of Yunnan Province | Fan N.,The Third Peoples Hospital of Yunnan Province | Liu X.,The Third Peoples Hospital of Yunnan Province
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2015

Exfoliation syndrome (XFS), one of the most common causes of glaucoma, represents an age related, complex, multifactorial and late-onset disease worldwide. The etiopathogenesis involves both genetic and environmental factor. However, the exact etiopathogenesis of XFS is still unclear. The purpose of this review was to discuss the recent research progress of the molecular genetics of XFS. Some candidate genes linked to XFS include lysyl oxidase-like 1 (LOXL1) gene, clusterin (CLU) gene, contactin associated protein-like 2 (CNTNAP2) gene, apolipoprotein E (ApoE) gene, matrix metallo proteinases (MMPs) gene, glutathione S-transferase (GST) gene, transforming growth factor-β1, (TGF-β1) gene, tumor necrosis factor-α (TNF-α) gene and so on. These genes may be modifying genes for the development of XFS. Copyright © 2015 by the Chinese Medical Association.


He Y.,Harbin Medical University | Han L.,Harbin Medical University | Li W.,The Third Peoples Hospital of Yunnan Province | Shu X.,University of Houston | And 4 more authors.
Gene | Year: 2012

The calcium-sensing receptor (CaSR) is involved in maintaining calcium homeostasis via the regulation of parathyroid hormone (PTH) secretion. The associations between serum calcium concentrations, parathyroid hormone (PTH) level and CaSR polymorphism A986S have been studied, but results are inconsistent. Therefore, we performed a meta-analysis to clarify the role of this polymorphism on this topic. Weighted mean difference (WMD) and 95% confidence interval (CI) were calculated with random-effects model or fixed-effects model based on the heterogeneity analysis. Overall 2820, 1135 and 3149 healthy individuals were included for total calcium concentration, ionized calcium concentration and PTH level meta-analyses, respectively. Most of the individuals in this meta-analysis were healthy women. Healthy individuals with the AS. +. SS genotype had significantly higher total and ionized calcium concentrations than those with the AA genotype. However, there was no statistical significance concerning serum PTH level. The pooled WMD for total calcium concentration was 0.028 (95% CI: 0.012-0.045, P = 0.001); for ionized calcium concentration was 0.016 (95% CI: 0.013-0.020, P < 0.0001); and for PTH level was - 0.027 (95% CI: -0.360-0.306, P = 0.874). In conclusion, our meta-analysis indicates that the CaSR A986S polymorphism might be associated with total and ionized calcium concentrations in healthy individuals. © 2011 Elsevier B.V.


PubMed | Northwestern University, Harvard University and The Third Peoples Hospital of Yunnan Province
Type: Journal Article | Journal: Journal of Alzheimer's disease : JAD | Year: 2016

Microglia are immune cells of the brain that display a range of functions. Most of our knowledge about microglia biology and function is based on cells from the rodent brain. Species variation in the complexity of the brain and differences in microglia response in the primate when compared with the rodent, require use of adult human microglia in studies of microglia biology. While methods exist for isolation of microglia from postmortem human brains, none allow culturing cells to high passage. Thus cells from the same case could not be used in parallel studies and multiple conditions. Here we report a method, which includes use of growth factors such as granulocyte macrophage colony stimulating factor, for successful culturing of adult human microglia from postmortem human brains up to 28 passages without significant loss of proliferation. Such cultures maintained their phenotype, including uptake of the scavenger receptor ligand acetylated low density lipoprotein and response to the amyloid- peptide, and were used to extend in vivo studies in the primate brain demonstrating that inhibition of microglia activation protects neurons from amyloid- toxicity. Significantly, microglia cultured from brains with pathologically confirmed Alzheimers disease displayed the same characteristics as microglia cultured from normal aged brains. The method described here provides the scientific community with a new and reliable tool for mechanistic studies of human microglia function in health from childhood to old age, and in disease, enhancing the relevance of the findings to the human brain and neurodegenerative conditions.


PubMed | Kunming University, The First Peoples Hospital of Yunnan Province, Kunming Medical University, Southwest University and The Third Peoples Hospital of Yunnan Province
Type: Journal Article | Journal: Oncotarget | Year: 2016

Ectopic glucose-6-phosphate dehydrogenase (G6PD) expression may contribute to tumorigenesis in cervical cancer associated with high-risk human papillomavirus (HR-HPV 16 and 18) infections. Here, we demonstrate that microRNA-1 (miR-1) in association with AGO proteins targets G6PD in HR-HPV-infected human cervical cancer cells. miR-1 inhibited expression of a reporter construct containing a putative G6PD 3-UTR seed region and suppressed endogenous G6PD expression. Down-regulation of miR-1 increased G6PD expression in cervical cancer cells. Regression analysis revealed that miR-1 levels correlate negatively with the clinicopathologic features in HR-HPV 16/18-infected cervical cancer patients. miR-1 overexpression inhibited proliferation and promoted apoptosis in cervical cancer cells and reduced xenograft tumor growth in nude mice. Conversely, sponge-mediated miR-1 knockdown markedly increased viability and reduced apoptosis in cervical cancer cells and supported neoplasm growth. Restoration of G6PD expression partially reversed the effects of miR-1 overexpression both in vitro and in vivo. In addition, co-transfection of G6PD siRNA and miR-1 sponge partially reversed miR-1 sponge-induced reductions in cell viability and neoplasm growth. These results suggest that miR-1 suppresses the development and progression of HR-HPV 16/18-infected cervical cancer by targeting G6PD and may be a promising novel therapeutic candidate.


PubMed | CAS Kunming Institute of Zoology and The Third Peoples Hospital of Yunnan Province
Type: Journal Article | Journal: Journal of human genetics | Year: 2016

In the past decades, the Tai people are increasingly being focused by genetic studies. However, a systematic genetic study of the whole Tai people is still lacking, thus making the population structure as well as the demographic history of this group uninvestigated from genetic perspective. Here we extensively analyzed the variants of hypervariable segments I and II (HVS-I and HVS-II) of mitochondrial DNA (mtDNA) of 719 Tai samples from 19 populations, covering virtually all of the current Tai peoples residences. We observed a general close genetic affinity of the Tai people, reflecting a common origin of this group. Taken into account the phylogeographic analyses of their shared components, including haplogroups F1a, M7b and B5a, our study supported a southern Yunnan origin of the Tai people, consistent with the historical records. In line with their diverse cultures and languages, substantial genetic divergences can be observed among different Tai populations that could be attributable to assimilation of maternal components from neighboring populations. Our study further implied the advent of rice agriculture in Mainland Southeast Asia at 5 kya (kilo years ago) had greatly promoted the population expansion of the Tai people.


PubMed | The Third Peoples Hospital of Yunnan Province, Kunming Medical University and Kunming Childrens Hospital
Type: | Journal: Oncology reports | Year: 2017

Pediatric head and neck cancers account for overall 12% of all pediatric cancers. Despite recent advances in therapeutic modalities, children with tumor metastasis have poor prognosis. Therefore, there is an unmet need for new and effective treatment modalities for pediatric head and neck cancers. The present study describes a simple and efficient method for fabrication of cationic lipidpolymer hybrid nanoparticles(CLPNs) for codelivery of cisplatin(CDDP) and DNA(CDDP/DNA CLPNs) for the therapy of childhood head and neck cancers. CDDP/DNA CLPNs were prepared by the modified double emulsion solvent evaporation method with selfassembly. CDDPloaded CLPNs(CDDP CLPNs), CDDP-loaded polymeric nanoparticles(PNPs)(CDDP PNPs), and DNAloaded Lipofectamine2000(DNA LIPO) were also prepared for comparison. The results illustrated that the concentration of the cationic lipid has influence on the characteristics of CLPNs. Invitro anticancer effect, invitro transfection efficiency, invivo antitumor and gene delivery efficacy of CDDP/DNA CLPNs have advantages over other formulations tested. In conclusion, outstanding delivery ability of CLPNs for both CDDP and DNA could combine the therapeutic efficiency of both drug and gene for the treatment of pediatric rhabdomyosarcoma(RMS).


PubMed | Cancer Hospital of Yunnan Province, Kunming Medical University and The Third Peoples Hospital of Yunnan Province
Type: Journal Article | Journal: Molecular medicine reports | Year: 2016

The present study aimed to explore the association between single nucleotide polymorphisms (SNPs) in the hepatocyte nuclear factor4 (HNF4) gene and the incidence of type 2 diabetes in the Chinese Bai population in Dali city, China. The polymerase chain reactionrestriction fragment length polymorphism method was used to analyze four SNPs (rs4810424, rs1884613, rs1884614 and rs2144908) in the HNF4 gene in 44 patients with type 2 diabetes and 87 healthy controls in Chinese Bai individuals. The haploid type was subsequently built to assess its association with the incidence of type 2 diabetes in the Bai population in Dali city. No significant differences were observed between the genotype and allele frequencies of the four SNPs in the HNF4 gene and type 2 diabetes mellitus (P>0.05). However, the frequency of haplotype, CCTA, built by rs4810424, rs1884613, rs1884614 and rs2144908 was significantly higher in the type 2 diabetes mellitus group compared with the control group (2=8.34, P=0.004). The four polymorphisms, rs4810424, rs1884613, rs1884614 and rs2144908, in the HNF4 gene were not the susceptible loci for type 2 diabetes in the Bai population of Dali city, however, the haplotype, CCTA, built from the four SNPs may increase the risk of type 2 diabetes in this population.

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