Third Peoples Hospital of Nantong

Nantong, China

Third Peoples Hospital of Nantong

Nantong, China
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Qiao J.,Third Peoples Hospital of Nantong | Dou Z.-H.,Third Peoples Hospital of Nantong | Wu F.,Nantong University | Meng G.-L.,Nantong University | And 2 more authors.
Chinese Pharmacological Bulletin | Year: 2014

Aim: To investigate the effects and mecha of ganoderma lucidum polysaccharides and metformin on pathological changes of thoracic aorta in diabetic rats and the mechanisms.Methods: SD rats were fed with high fat diet for 4 weeks and injected with strepto-zotocin (30mg · kg-1) to replicate type 2 diabetic model. The diabetic rats were randomly into diabetes group, ganoderma lucidum polysaccharides group(600 mg · kg-1) ,metformin group(600 mg · kg-1) combination group (ganoderma lucidum polysaccharides 300 mg · kg-1 + metformin 300 mg · kg-1) and normal control group. After 12 weeks' treatment, the levels of fasting serum glucose, the activity of catalase(CAT), glutathione peroxidase (GSH-Px), total cholesterol (TC) and triglyceride (TG) in serum were detected. Pathological changes of thoracic aorta were observed by HE staining. Immunohistochemy and Western blot were used to detect thoracic aorta VEGF protein expression.Results: Combination group could lower fasting serum glucose and blood fat significantly, meanwhile the activity of CAT and GSH-Px in serum was improved. The expression of VEGF in thoracic aorta was repressed. The result of HE staining suggested that the lipid deposits in aortic endothelium in combination group were less than those in the model group.Conclusions: Ganoderma lucidum polysaccharides combined with metformin has an obvious prevention on pathological changes of thoracic aorta in diabetic rats. The possible mechanism may be related to repressing oxidative stress of thoracic aorta, regulating the dyslipidemia, and the down regulation of the expression of VEGF in thoracic aorta.


Qiao J.,Third Peoples Hospital of Nantong | Dou Z.-H.,Third Peoples Hospital of Nantong | Wu F.,Nantong University | Meng G.-L.,Nantong University | And 2 more authors.
Chinese Pharmacological Bulletin | Year: 2014

Aim: To study the effects of ganoderma lucidum polysaccharides and metformin on myocardial fibrosis of type 2 diabetic rats and its mechanism. Methods: SD rats were fed with high fat diet for 4 weeks, and then were injected with streptozotocin (30mg • kg-1) to replicate type 2 diabetic model. The diabetic rats were randomized into normal control group, diabetes group, ganoderma lucidum polysaccharides group (600 mg • kg-1), metformin group (600 mg • kg-1), and combination group (ganoderma lucidum polysaccharides 300 mg • kg-1 + metformin 300 mg • kg-1). After 12 weeks' treatment, the levels of fasting serum glucose were determined and the extent of myocardial fibrosis was observed by Picro-sirius red staining. The contents of AGEs in serum were determined by fluorescence spectrophotometer. The activities of CAT and GSH-Px in myocardium were detected. Immunohistochemical method and Western blot were used to detect myocardial tissue AGEs and CTGF protein expression. Results: Combination group could repress patho-proceeding of myocardial fibrosis efficiently, improve the activity of CAT and GSH-Px in myocardium and lower the concentration of AGEs in serum, as well as reduce the expression of AGEs and CTGF in myocardium. Conclusions: Ganoderma lucidum polysaccharides and metformin could prevent myocardial fibrosis. The possible mechanism may be related to repressing oxidative stress of myocardium, lowering serum AGEs and down regulating AGEs and CTGF of myocardium.


Zhang X.,Nantong University | Cheng S.,Nantong University | Ding H.,Nantong University | Wu H.,Nantong University | And 2 more authors.
Proceedings - 2016 8th International Conference on Information Technology in Medicine and Education, ITME 2016 | Year: 2017

In this paper, a method for removing ultrasound medical image noise is proposed. This method improves the original median filter by designing a suit of directional templates. In order to obtain local directions, a filter, which combines Gaussion blur with direction parameter, is designed. Then, directional templates based on local directions mentioned above are used as the filter for reducing spark noise. At last, the finial denoising result of each pixel is obtained from the filtered image or the original noise-polluted image by judging their differences. If the difference between two corresponding pixels of the two images is less than a threshold, the current pixel value of the noise-polluted image is kept. Otherwise, the one of the filtered image is used. The proposed method preserves the contour and the texture areas, which increases visual effects. Experiment results show the proposed method achieves sound performance on the synthetic images created by Field II. © 2016 IEEE.


Qiao J.,Third PeopleS Hospital of Nantong | Dou Z.-H.,Third PeopleS Hospital of Nantong | Wu F.,Jiangsu University | Meng G.-L.,Jiangsu University | And 2 more authors.
Chinese Pharmacological Bulletin | Year: 2016

Aim To discuss the effects and mechanism of Ganoderma lucidum polysaccharides and metformin on myocardial structure and hemodynamics in type 2 diabetic rats. Methods High fat diet combined with intraperitoneal injection of low dose streptozotocin 30 mg • kg"1 was applied to establish rat model of type 2 diabetes mellitus. The diabetic rats were randomly into normal control group, diabetes group, ganoderma lucidum polysaccharides group(600 mg • kg-1) , metformin group (600 mg • kg-1), combination group ( ganoderma lucidum polysaccharides 300 mg 'kg-1 + metformin 300 mg • kg-1). After 12 weeks' treatment,the levels of fasting serum glucose were determined and the hemodynamic parameters ( LVSP, LVEDP, dp/dfmax, - dp/dtmax) were determined. Collagen volume fraction (CVF) was detected by Van Gieson. Immunohisto- chemical method and Western blot were used to detect myocardial tissue MMP-2 protein expression. Results The fasting blood glucose was significantly decreased in the combined treatment group. Combined medication could significantly improve hemodynamic parameters in diabetic rats; reduced LVEP and raised LVEDP, dp/ dtmax and -dp/dtmax. CVF was significantly decreased in combination group. The expression of MMP-2 in my-ocardial tissue was significantly inhibited. Conclusions The combination of Ganoderma lucidum polysaccharide and metformin can significantly improve the hemodynamic parameters in type 2 diabetic rats, and have a preventive effect on diabetic cardiomyopathy. The mechanism may be related to the down regulation of the expression of MMP-2.


Shi Y.,Third Peoples Hospital of Nantong | Chen X.,Third Peoples Hospital of Nantong
Pharmaceutical Care and Research | Year: 2014

Objective: To explore contents and methods of pharmaceutical care by clinical pharmacists with optimized pharmaceutical care process as an entry point.Methods: The purposes, forms, contents, focuses and efficiency of optimized pharmaceutical care process were described, and then typical cases were analyzed in detail.Results: Through the implementation of optimized pharmaceutical care process, patients, medical staffs and clinical pharmacists all benefitted quite substantially from it.Conclusion: Optimized pharmaceutical care process could be used by clinical pharmacists as an entry point to carry out pharmaceutical care, and is also a reflection of the value of clinical pharmacists.


Su Y.-M.,Nantong University | Li J.,Jiangnan University | Guo Y.-F.,Jiangnan University | Cai F.,Third Peoples Hospital Of Nantong | And 4 more authors.
Clinical Laboratory | Year: 2015

Background: MicroRNAs are small, single-stranded, non-protein-coding RNAs of about 22 nucleotides. Micro- RNA molecules have been identified to play key roles in a broad range of physiologic and pathologic processes. Polymorphisms in the corresponding sequence space are likely to make a significant contribution to phenotypic variation. A T/C genetic variant (rsll614913) in the pre-miR-196a2 sequence could alter mature miR-196a expression and target mRNA binding. The aim of the present study is to evaluate the relationship between this polymorphism and atrial fibrillation (AF). Methods: A total of 123 participants were enrolled, 65 AF patients were confirmed with electrocardiogram (ECG) or dynamic electrocardiography, 58 normal individuals were assigned to the control group. Genotypes of the pre- miR-196a2 were distinguished using the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Results: The distribution of the pre-miR-196a2 genotypes (TT, TC, and CC) was 15.38%, 46.15%, and 38.46% in the AF group and 39.66%, 46.55%, and 13.79% in the controls, respectively (p = 0.0011). Compared with the TT genotype, the C allele carriers (TC+CC genotypes) had a 3.968-fold increased risk of AF (adjusted OR = 3.968, 95% CI = 1.633 - 9.644, p = 0.002). AF patients with the TC+CC genotype had greater left atrial dimension than did patients with the TT genotype (42.10 ± 8.74 vs. 35.13 ± 8.16, p = 0.0224). Conclusions: Our data support that the pre-miR-196a2 polymorphism is associated with AF, and the C allele is a risk factor for AF.


Zhang J.,Nantong University | Jiang W.,Third Peoples Hospital of Nantong | Liu W.,Nantong University | Wu J.-J.,Nantong University | And 5 more authors.
Future Oncology | Year: 2016

Aim: We investigated the effects of TORC1/2 kinase inhibitors on colorectal cancer (CRC) cell lines. Materials & methods: Using selective TORC1/2 inhibitors, rapamycin and PP242, we assessed their effect on the growth of CRC cells in vitro and tumor growth in vivo. Results: Rapamycin and PP242 inhibit proliferation and induce apoptosis of CRC cells. They also enhance proapoptotic effect of conventional chemo drug doxorubicin in CRC cells in vitro. When combined with doxorubicin, rapamycin and PP242 almost completely inhibit tumor growth in vivo. Rapamycin and PP242 inhibit phosphorylation of Akt, ribosomal S6 kinase, 4EBP1 and mTOR. Conclusion: Our study suggests rapamycin and PP242 may be a useful therapeutic agent and inhibiting mTOR signaling pathway represents a new targeted therapy for CRC. © 2016 Future Medicine Ltd.


Sun L.,Nantong University | Qiang R.,Third Peoples Hospital of Nantong | Yang Y.,Nantong University | Jiang Z.-L.,Nantong University | And 5 more authors.
PLoS ONE | Year: 2014

The present study was conducted to clarify whether treatment with L-serine can improve the brain repair and neurorestoration of rats after permanent middle cerebral artery occlusion (pMCAO). After pMCAO, the neurological functions, brain lesion volume, and cortical injury were determined. GDNF, NGF, NCAM L1, tenascin-C, and Nogo-A levels were measured. Proliferation and differentiation of the neural stem cells (NSCs) and proliferation of the microvessels in the ischemic boundary zone of the cortex were evaluated. Treatment with L-serine (168 mg/kg body weight, i.p.) began 3 h after pMCAO and was repeated every 12 h for 7 days or until the end of the experiment. L-Serine treatment: 1) reduced the lesion volume and neuronal loss; 2) improved the recovery of neurological functions; 3) elevated the expression of nerve growth-related factors; and 4) facilitated the proliferation of endogenous NSCs and microvessels activated after pMCAO and increased the number of new-born neurons. 5) D-cycloserine, an inhibitor of serine hydroxymethyltransferase, blunted the effects of L-serine on NSC proliferation, differentiation, microvascular proliferation. In conclusions, L-serine treatment in pMCAO rats can reduce brain injury and facilitate neurorestoration which is partly associated with the improvement of proliferation of NSCs and microvessels, reconstruction of neurovascular units and resultant neurorepair. The effects of L-serine on endogenous NSC proliferation and microvascular proliferation are partly mediated by the action of L-serine as a substrate for the production of one-carbon groups used for purine and pyrimidine synthesis and modulation of the expression of some nerve growth-related factors. © 2014 Sun et al.


Zhang J.-D.,Third Peoples Hospital of Nantong | Cai C.-P.,Third Peoples Hospital of Nantong
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2012

Background: To study diabetic retinopathy (DR) related risk factors is very important in the prevention of DR. Platelet endothelial cell adhesion molecule-1 (PECAM-1) is an important mediator that mediates high blood glucose-induced vascular diseases in diabetic patients. However, its mechanism is still below understood. Objective: This clinical study was to investigate the effect of serum level changes of PECAM-1 on DR in type 2 diabetic patients. Methods: Fifty-four patients with type 2 diabetes were enrolled from the endocrinology department of the Third People's Hospital of Nantong City. Fundus examination was performed using the ophthalmoscope and fundus fluorescence angiography (FFA) on all the patients, and these patients were grouped as the non-DR (NDR) group (18 cases), non-proliferative DR(NPDR) group (20 cases) and proliferative DR group (PDR) (16 cases) based on the DR staging criterion of the Chinese Medical Association (1987 version). Eighteen age- and gender-matched normal subjects served as the normal control group. Peripheral blood was collected, and serum PECAM-1 levels were assayed using ELISA. Serum HbA1c levels were detected using the high performance liquid colorimetric(HPLC) method. The correlation of serum PECAM-1 level with serum HbA1c level was analyzed. All results were compared among the groups. Results: The serum PECAM-1 levels were (10.907 ± 2.792), (7.024 ± 2.377), (5.231 ± 1.816) and(3.817± 1.045) μg/L, respectively, in the PDR group, NPDR group, NDR group and normal control group, showing a significant difference among the 4 groups (F= 12.630, P = 0.02). Serum PECAM-1 content was significantly higher in the PDR group when compared with the NPDR group, NDR group and normal control group (P<0.05). The serum HbA1c levels were (12.596 ± 3.148)%, (9.118 ± 3.356)%, (5.491 ± 1.017)% and (4.992 ± 0.725)% in the PDR group, NPDR group, NDR group and normal control group, respectively, with a significant difference among these 4 groups (F = 7.130, P = 0.015), and those in the PDR group and NPDR group were significantly elevated in comparison with the NDR group and normal control group (P<0.05). Significantly positive correlations were seen between serum PECAM-1 level and HbA1c level in the PDR group, NPDR group and NDR group (r = 0.799, P<0.01; r = 0.647, P<0.01; r = 0.685, P<0.01). Significantly more patients with a disease course of ≥ 10 years were in the NPDR group in comparison with the PDR group (P = 0.023). Conclusions: Increase of serum PECAM-1 level is closely associated with blood glucose level, and it is an important factor in the pathogenesis and development of DR. These results imply that control of blood glucose is crucial for the prevention of DR in patient with type 2 diabetes. Copyright © 2012 by the Chinese Medical Association.


Hu B.-Y.,Nantong University | Liu X.-J.,Third Peoples Hospital of Nantong | Qiang R.,Third Peoples Hospital of Nantong | Jiang Z.-L.,Nantong University | And 4 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacological relevance Ginseng, the root of Panax ginseng C.A. Meyer, is a traditional medicinal herb that has been widely used in Asia for the treatment of many diseases through its effects of reinforcing vitality, strengthening the bodily resistance to pathogenic factors, engendering body liquids and allaying thirst, relieving uneasiness of the body and mind and benefiting intelligence, reducing body weight and prolonging life. Ginsenosides are the most important biologically active substances in ginseng. Many reports have suggested that ginsenosides could exert prominent neuroprotective and neurotrophic effects, promote neural stem/progenitor cell (NSC) proliferation and promote neurite outgrowth and neuronal network formation. The present study aimed to investigate whether treatment with ginsenosides could facilitate NSC proliferation in the hippocampal formation after traumatic brain injury (TBI) and contribute to the recovery of neurological functions including learning and memory. Materials and methods The modified Feeneys method was used to induce a TBI in rats. Ginseng total saponins (GTS) were treated intraperitoneally twice a day for 1 week after the TBI. The neurological functions, morphology of the hippocampus, expression of nerve growth-related factors and number of NSCs in the hippocampal formation ipsilateral to the trauma were determined. Results We determined 1) GTS (5-80 mg/kg) treatment after a TBI improved the recovery of neurological functions, including learning and memory, and reduced cell loss in the hippocampal area. The effects of GTS at 20, 40, 60, and 80 mg/kg were better than the effects of GTS at 5 and 10 mg/kg. 2) GTS treatment (20 mg/kg) after a TBI increased the expression of NGF, GDNF and NCAM, inhibited the expression of Nogo-A, Nogo-B, TN-C, and increased the number of BrdU/nestin positive NSCs in the hippocampal formation. Conclusions GTS treatment in rats after a TBI alleviated the secondary brain injury and ameliorated the neurological functions with an effective dose limit of 5-80 mg/kg. GTS regulated the expression of nerve growth-related factors and improved the proliferation of neural stem/progenitor cells, which might facilitate neural regeneration and tissue repair, and might contribute to the recovery of neurological functions, including learning and memory. These effects of GTS might provide a foundation for the use of ginseng as a medicinal herb to enhance intelligence, reduce the aging process and prolong life in the traditional medicine. © 2014 Elsevier Ireland Ltd.

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