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Li Q.,Chongqing Medical University | Lv F.,Chongqing Medical University | Yao G.,Third Military Medical University | Li Y.,Chongqing Medical University | Xie P.,Chongqing Medical University
Acta Radiologica | Year: 2014

Background: To assess the diagnostic performance of 64-section computed tomography angiography (CTA) for detection of cerebral aneurysms by comparison with the new gold standard three-dimensional (3D) digital subtraction angiography (DSA). Material and Methods: A total of 128 patients who underwent both 64-section CTA and 3D DSA for suspected intracranial aneurysms were included. The location, size, and shape of the aneurysm were assessed and compared with 3D DSA results. The sensitivity, specificity, positive and negative predictive values of 64-section CTA were calculated by using 3D DSA as reference standard. Results: According to the reference standard, a total of 145 aneurysms were detected at 3D DSA in 118 patients. Of these 145 aneurysms, 64-section CTA detected 139 aneurysms in 118 patients. The sensitivity of CTA for detection of aneurysm was 95.8% on a per-aneurysm basis. All missed aneurysms were non-causative very small aneurysms in patients harboring multiple aneurysms. On a per-patient basis, the sensitivity, specificity, positive and negative predictive values were 100%. Conclusion: Compared with the new gold standard 3D DSA, 64-section CTA offers high sensitivity and specificity for detection of intracranial aneurysms. It could be readily used as a screening imaging method for detection of intracranial aneurysms. © The Foundation Acta Radiologica 2013.

Li P.,Chongqing Medical University | Zhou C.,Chongqing Medical University | Xu L.,Chongqing Medical University | Xiao H.,Third Military Medical University
International Journal of Medical Sciences | Year: 2013

Objective: To investigate the relationship between hypoxia and in vitro "stemness" of cancer stem cells (CSCs). Methods: U87 cells, U251 cells and primary glioma cells (n=3) experienced hypoxia. Transmission electron microscopy was done to detect the ultrastructure of these cancer cells; MTT assay to detect the cell growth; flow cytometry to detect cell cycle and CD133 expression; Transwell chamber assay was carried out to detect the cell migration; colony-forming assay to detect the colony-forming efficiency; real-time quantitative PCR and Western blot were carried out to detect the mRNA and protein expression of markers of stem cells and their differentiation, respectively. Results: Hypoxia maintained the undifferentiated state of primary glioma cells, slowed down the growth of glioma cells which were in a relatively quiescent stage, increased the colony forming efficiency and migration of glioma cells, and elevated the expression of markers of stem cells, but the expression of markers for stem cell differentiation was reduced after hypoxia treatment. Conclusion: Hypoxia may induce the dedifferentiation of differentiated glioma cells which then acquire the stemness. © Ivyspring International Publisher.

Li J.,PLA Air Force Aviation University | Wang K.,Third Military Medical University | Yang Y.,PLA Air Force Aviation University
Mathematical and Computer Modelling | Year: 2011

In this paper, a hepatitis B virus (HBV) infection model with logistic hepatocyte growth is investigated, and the basic reproduction number of HBV determining the extinction and the persistence of infection is obtained. When the basic reproduction number is less than one, the infection becomes extinct eventually; when the basic reproduction number is greater than one, the infection becomes persistent in vivo. Hopf bifurcation surface determining the stability of the chronic infection equilibrium is found, and the effect of all the parameters on stability of the chronic infection equilibrium is also discussed. © 2011.

Yoshikai N.,Nanyang Technological University | Wei Y.,Third Military Medical University
Asian Journal of Organic Chemistry | Year: 2013

Pyrroles, indoles, and carbazoles are among the most important families of nitrogen-containing heterocycles that occur frequently in natural products, pharmaceuticals, agrochemicals, and other functional molecules. Consequently, improved syntheses of these compounds continue to interest synthetic chemists. This Focus Review describes recent advances in synthetic methods for producing these privileged heterocycles that feature transition-metal-catalyzed C-H activation approaches. Because of the common five-membered pyrrole core, some of the C-H activation approaches are applicable to two or more of the pyrrole, indole, and carbazole skeletons. The reactions discussed here not only serve as atom- and step-economical alternatives to the existing synthetic methods, but also show the latest developments in organometallic chemistry and homogeneous catalysis. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Tianjin Chasesun Pharmaceutical Co. and Third Military Medical University | Date: 2011-03-21

The use of Kukoamine A and Kukoamine B in the preparation of drugs for the prevention and treatment of sepsis and autoimmune disease is disclosed. Bacterial endotoxin/lipopolysaccaride (LPS) and unmethylated DNA (CpG DNA) of bacteria, the major pathogen-associated molecular patterns in sepsis and autoimmune disease, are specifically targeted, while the disclosed use directionally isolates lead compounds from traditional Chinese medicine. These measures can overcome the major defects of uncertainty of pharmacological material basis and drug targets of extracts and constituents of traditional Chinese medicine. The disclosed use can help in developing a safe, effective and quality controllable drug for prevention and treatment of sepsis and autoimmune disease so as to help solve the present lack of effective drugs in clinical treatment.

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