Third Hospital of Xiamen

Xiamen, China

Third Hospital of Xiamen

Xiamen, China
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Liu J.-M.,Zhangzhou Normal College | Lin L.-P.,Zhangzhou Normal College | Liu Z.-B.,Third Hospital of Xiamen | Yang M.-L.,Zhangzhou Normal College | And 4 more authors.
Journal of Fluorescence | Year: 2012

The labelling reagent CdSe@CdS-QDs-Cys (QDs-Cys) with the grain diameter of 4.5 nm was synthesized by modifying CdSe@CdS quantum dots (QDs) with cysteine (Cys). At the same time, QDs-Cys-AbIgE, a phosphorescent quantum dot probe, was developed based on the labelling reaction between -COOH of QDs-Cys and -NH 2 of goat anti human IgE antibody (Ab IgE). This probe with excellent biocompatibility and high specificity could not only emit strong and stable room temperature phosphorescence (RTP), but also could carry out specific immunoassay (IA) with immunoglobulin E (IgE), causing the RTP of the system to sharply enhance. Thus, a new solid substrate room temperature phosphorescence immunoassay (SSRTPIA) for the determination of IgE was established. The limit of quantification (LOQ) of the method was 0.12 fg spot -1, corresponding concentration was 3.0×10 -13 g mL -1 and sampling quantity was 0.40 μL spot -1. This highly selective, sensitive and accurate SSRTPIA has been applied to determine IgE in biological samples and diagnose diseases, and the results agreed well with those obtained by enzyme-link immunoassay (ELISA). Meanwhile, the mechanisms of QDs-Cys labelling Ab IgE and the determination of IgE by SSRTPIA were also discussed. © 2011 Springer Science+Business Media, LLC.

Lin J.-M.,Zhangzhou Normal University | Huang Y.-Q.,Zhangzhou Normal University | Huang Y.-Q.,Fujian Medical University | Liu Z.-B.,Third Hospital of Xiamen | And 3 more authors.
RSC Advances | Year: 2015

Formaldehyde (HCHO) could reduce Ag+ to Ag on the surface of gold nanorods (AuNRs) to form Au core-Ag shell nanorods (Au@AgNRs) in a AuNRs-Ag+-HCHO system, which caused the dielectric function to change, the longitudinal plasmon absorption band (LPAB) of AuNRs to redshift (ΔλLPAB) and the color of the solution to change obviously. Thus, a responsive, simple, sensitive and selective AuNRs colorimetric sensor for the determination of HCHO has been developed based on the linear relationship between ΔλLPAB and the concentration of HCHO. The limit of detection (LOD) of this sensor is 6.3 × 10-11 (g mL-1), which is much lower than that of surface-enhanced Raman spectroscopy (SERS), showing its high sensitivity. What's more, the sensor has been applied to the detection of HCHO in water samples with the results agreeing well with resonance fluorescence spectrometry, showing its great practicality. Furthermore, the morphological changes of AuNRs and Au@AgNRs were characterized by transmission electron microscopy (TEM) and the sensing mechanism for the detection of HCHO was also discussed. © The Royal Society of Chemistry 2015.

Liu J.-M.,Zhangzhou Normal College | Huang X.-M.,Zhangzhou Normal College | Huang X.-M.,China Institute of Technology | Liu Z.-B.,Third Hospital of Xiamen | And 7 more authors.
Journal of Fluorescence | Year: 2011

Using Pb 2+ as ion perturber, phenosafranine (PF) and fluorescein isothiocyanate (FITC) could emit strong and stable room temperature phosphorescence (RTP) signal on the filter paper, respectively. When they were mixed, the phenomenon that the RTP signal of PF and FITC enhanced significantly was found. And 1.12 ag DNA spot -1 (sample volume was 0.40 μL, corresponding concentration was 2.8∈×∈10 -15 g mL -1) could cause the RTP signal of both PF and FITC to enhance sharply. The content of DNA was proportional to the ΔI p of PF and FITC in the system at 634 and 659 nm. Thus, a new solid substrate room temperature phosphorimetry (SSRTP) for the determination of trace DNA was established by using FITC-PF as double-luminescent phosphorescence probe. The detection limit (LD) of this method calculated by 3S b/k was 14 zg DNA spot -1 for PF and 18 zg DNA spot -1 for FITC, respectively, showing high sensitivity. It has been applied to the determination of trace DNA in practical samples and the analysis results were in accordance with those of fluorescence probe. The reaction mechanism of SSRTP for the determination of trace DNA was also discussed. © 2010 The Author(s).

Tan G.-W.,Xiamen University | Lan F.-L.,Third Hospital of Xiamen | Gao J.-G.,Third Hospital of Xiamen | Jiang C.-M.,Third Hospital of Xiamen | And 10 more authors.
Cancer Investigation | Year: 2012

Previously, we developed an orthotopic xenograft model of human glioblastoma multiforme (GBM) with high EGFR expression and invasiveness in Balb/c nu/nu nude mice. Now we also developed the same orthotopic xenograft model in transgenic nude mice with green fluorescent protein (GFP) expression. The present orthotopic xenografts labeled by phycoerythrin fluorescing red showed high EGFR expression profile, and invasive behavior under a bright green-red dual-color fluorescence background. A striking advantage in the present human GBM model is that the change of tumor growth can be observed visually instead of sacrificing animals in our further antitumor therapy studies. Copyright © 2012 Informa Healthcare USA, Inc.

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