Third Hospital of Wuhan

Wuhan, China

Third Hospital of Wuhan

Wuhan, China
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Chen Y.,Third Hospital of Wuhan | Lin L.,Guangzhou University of Chinese Medicine
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To establish the HPLC fingerprint of flavonoids of the six clinical frequently used Chinese materia medica for regulating Qi flow, such as Citri grandis, C. grands, Citri Reticulatae Pericarpium, Citri Reticulatae Pericarpium Viride, Aurantii Fructus, and Aurantii Fructus Immaturus from Citrus, and analysis differences in the fingerprints to provide scientific basis for profile-effect research and clinical reasonable use. Method: HPLC was performed on a C 18 column with methanol-water (with acetic acid), to establish HPLC fingerprints of the six kinds of medicinal herbs on the same chromatograph condition. Result: The six frequently used Chinese materia medica were divided into naringin type and hesperidin type according to the method of phytochemotaxonomy. Based on the retention time of chromatograph peaks, C. grandis and C. grands had fifteen common peaks; Citri Reticulatae Pericarpium, Citri Reticulatae Pericarpium Viride, Aurantii Fructus and Aurantii Fructus Immaturus had ten common peaks. All herbs had five common peaks. Compared with mutual model, the holistic similarity of chromatograms of C. grandis and C. grands was in the range of 0.9285-0.9962. The degree of similarity was high. For Citri Reticulatae Pericarpium, Citri Reticulatae Pericarpium Viride and Aurantii Fructus Immaturus, it was in the range of 0.9221-0.9973 and high. But the similarity of Aurantii Fructus was only in 0.4547-0.7733 with the mutual model. Conclusion: The established fingerprints of flavonoids of the six common traditional Chinese medicines can be used to compare the differences intuitively. Meanwhile, the peak height and peak areas of characteristic peaks are different remarkably, but whether it is connected with the different function of regulating Qi flow of the six medical materials in clinical use, is still needed to be studied.

Fan L.,Wuhan University of Technology | Wang L.,Wuhan University of Technology | Gao S.,Wuhan University of Technology | Wu P.,Wuhan University of Technology | And 4 more authors.
Carbohydrate Polymers | Year: 2011

In order to develop a wound healing material possessing anticoagulant activity, antimicrobial activity and moisture absorbability and moisture-retention capacity, carboxymethyl κ-carrageenan (CMKC) was synthesized by the reaction of κ-carrageenan with monochloroacetic acid. The products were characterized by FT-IR, 13C NMR, degree of substitution (DS) and molecular weight. Anticoagulant activity of CMKC was investigated by APTT, TT and PT assays. The results showed that CMKC with a low DS promoted anticoagulant activity in comparison with κ-carrageenan, but as the DS further increased from 0.42 to 1.09, the activity decreased. Antibacterial activity was evaluated and we found that the introduction of carboxymethyl groups conferred antibacterial activity onto κ-carrageenan. Results indicated that CMKC exhibited good antimicrobial properties against Escherichia coli and Staphylococcus aureus, and the antibacterial activity of CMKC enhanced as the DS increased. CMKC displayed better moisture-absorption and water-retention ability than κ-carrageenan and as the DS increased, these properties of CMKC increased. © 2011 Elsevier Ltd. All rights reserved.

Fan L.,Wuhan University of Technology | Jiang L.,Wuhan University of Technology | Xu Y.,Wuhan University of Technology | Zhou Y.,Wuhan University of Technology | And 4 more authors.
Carbohydrate Polymers | Year: 2011

Sodium alginate sulfates prepared from sodium alginate through reaction with an uncommon sulfating agent (N (SO3Na)3) which was synthesized by sodium bisulfite and sodium nitrite in aqueous solution. The factors that could affect the degree of substitution (DS) of sodium alginate sulfates were investigated in detail. A sodium alginate sulfate with DS of 1.87 was obtained under optimal conditions. The structures of the derivatives were characterized by FTIR and 13C NMR. FTIR spectra showed the characteristic absorptions of sulfate ester bonds at 1249 cm-1 and 873 cm-1. The in vitro coagulation assay of human plasma containing the sodium alginate sulfates was determined with respect to activated partial thromboplastin time (APTT), thrombin time (TT) and prothombin time (PT). These activities strongly depended on the DS, molecular weight (Mw) and the concentration of sodium alginate sulfates. The introduction of sulfate groups to hydroxyl groups greatly prolonged the APTT and TT. Low S% and concentration sodium alginate sulfates showed little anticoagulant activity. The high DS and concentration could inhibit the activity of IIa and Xa to prolong APTT and TT. The low molecular weight resulted in higher anti-factor Xa activity to promote anticoagulant activity. Generally, the introducing of sulfate groups could not increase PT, it had little effect on coagulation factors in the extrinsic pathway. © 2010 Elsevier Ltd. All rights reserved.

Gong H.,Huazhong University of Science and Technology | He Z.,Huazhong University of Science and Technology | Peng A.,Third Hospital of Wuhan | Zhang X.,Huazhong University of Science and Technology | And 5 more authors.
Scientific Reports | Year: 2014

Protein misfolding and aggregation are associated with more than twenty diseases, such as neurodegenerative diseases and metabolic diseases. The amyloid oligomers and fibrils may induce cell membrane disruption and lead to cell apoptosis. A great number of studies have focused on discovery of amyloid inhibitors which may prevent or treat amyloidosis diseases. Polyphenols have been extensively studied as a class of amyloid inhibitors, with several polyphenols under clinical trials as anti-neurodegenerative drugs. As oxidative intermediates of natural polyphenols, quinones widely exist in medicinal plants or food. In this study, we used insulin as an amyloid model to test the anti-amyloid effects of four simple quinones and four natural anthraquinone derivatives from rhubarb, a traditional herbal medicine used for treating Alzheimer's disease. Our results demonstrated that all eight quinones show inhibitory effects to different extent on insulin oligomerization, especially for 1,4-benzoquinone and 1,4-naphthoquinone. Significantly attenuated oligomerization, reduced amount of amyloid fibrils and reduced hemolysis levels were found after quinones treatments, indicating quinones may inhibit insulin from forming toxic oligomeric species. The results suggest a potential action of native anthraquinone derivatives in preventing protein misfolding diseases, the quinone skeleton may thus be further explored for designing effective anti-amyloidosis compounds.

Lei X.,Renmin University of China | Liu M.,Third Hospital of Wuhan | Yang Z.,Renmin University of China | Ji M.,Renmin University of China | And 2 more authors.
Digestive Diseases and Sciences | Year: 2012

Background: Thymoquinone (TQ), an active ingredient of the seed oil extract of Nigella sativa Linn, has previously been shown to possess antitumor, antioxidant, and anti-inflammatory bioactivity. Whether TQ has any effect on colitis remains controversial. Aim: The aim of this study was to determine whether treatment with TQ prevents and ameliorates colonic inflammation in a mouse model of inflammatory bowel disease. Methods: C57BL/6 murine colitis was induced by the administration of dextran sodium sulfate (DSS) (3 % W/V) in the drinking water supplied to the mice for 7 consecutive days. The mice with colitis were treated with 5, 10, or 25 mg/kg TQ orally, and changes in body weight and macroscopic and microscopic colitis scores were examined. In addition, biochemical analyses were conducted. Results: The treatment of mice with TQ prevented and significantly reduced the appearance of diarrhea and body weight loss. These results were associated with amelioration of colitis-related damage, as measured by macroscopic and microscopic colitis scores. In addition, there was a significant reduction in colonic myeloperoxidase activity and malondialdehyde levels and an increase in glutathione levels. Conclusions: These results indicate that TQ administration can prevent and improve murine DSS-induced colitis. These findings suggest that TQ could serve as a potential therapeutic agent for the treatment of patients with inflammatory bowel disease. © 2012 Springer Science+Business Media, LLC.

Li J.,Third Hospital of Wuhan | Wang X.,Third Hospital of Wuhan | Zhang Z.,Third Hospital of Wuhan | Zou J.,Third Hospital of Wuhan | And 2 more authors.
Current Medical Research and Opinion | Year: 2014

Purpose: To investigate the distribution of statin therapy correlated gene polymorphism allele CYP2D6*10 (C188T) in Ningxia Hui nationality and to discuss the relationship between the gene polymorphism allele CYP2D6*10 (C188T) and the lipid-lowering efficacy of simvastatin and hyperlipidemia. Materials and methods: One hundred and fifty healthy people (80 males and 70 females) and 200 hyperlipidemia patients (105 males and 95 females) were recruited at Ningxia People's Hospital. Genotypes were determined by using allele-specific amplification (ASA-PCR) in 150 healthy controls and 200 patients with hyperlipidemia. Relationships between genotypes and lipid levels and the lipid-lowering efficacy of simvastatin were analyzed. Results: The distribution frequency of the CYP2D6*10 allele was 47.6% in the Ningxia Hui nationality. There was no significant correlation between the CYP2D6*10 allele and hyperlipidemia. At 8 weeks after treatment with simvastatin, total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels were more obviously decreased with the CC genotype than the CT and TT genotypes, with significant differences. Conclusion: Distribution of the gene polymorphism of CYP2D6*10 is ethnically and geographical different. The allele T of CYP2D6*10 is not related to hyperlipidemia. CC expression seemed to increase the lipid-lowering effects of simvastatin. The hyperlipidemia patients with CC genotype should take lower doses of simvastatin than those with CT/TT genotype in the Ningxia Hui population. © 2014 Informa UK Ltd.

Maixner D.W.,University of Georgia | Yan X.,University of Georgia | Yan X.,Third Hospital of Wuhan | Gao M.,University of Georgia | And 2 more authors.
Anesthesiology | Year: 2015

Background: Neuroinflammation and dysfunctional glial glutamate transporters (GTs) in the spinal dorsal horn are implicated in the genesis of neuropathic pain. The authors determined whether adenosine monophosphate-activated protein kinase (AMPK) in the spinal dorsal horn regulates these processes in rodents with neuropathic pain. Methods: Hind paw withdrawal responses to radiant heat and mechanical stimuli were used to assess nociceptive behaviors. Spinal markers related to neuroinflammation and glial GTs were determined by Western blotting. AMPK activities were manipulated pharmacologically and genetically. Regulation of glial GTs was determined by measuring protein expression and activities of glial GTs. Results: AMPK activities were reduced in the spinal dorsal horn of rats (n = 5) with thermal hyperalgesia induced by nerve injury, which were accompanied with the activation of astrocytes, increased production of interleukin-1β and activities of glycogen synthase kinase 3β, and suppressed protein expression of glial glutamate transporter-1. Thermal hyperalgesia was reversed by spinal activation of AMPK in neuropathic rats (n = 10) and induced by inhibiting spinal AMPK in naive rats (n = 7 to 8). Spinal AMPKα knockdown (n = 6) and AMPKα1 conditional knockout (n = 6) induced thermal hyperalgesia and mechanical allodynia. These genetic alterations mimicked the changes of molecular markers induced by nerve injury. Pharmacological activation of AMPK enhanced glial GT activity in mice with neuropathic pain (n = 8) and attenuated glial glutamate transporter-1 internalization induced by interleukin-1β (n = 4). Conclusions: These findings suggest that enhancing spinal AMPK activities could be an effective approach for the treatment of neuropathic pain. © 2015, the American Society of Anesthesiologists, Inc. Wolters Kluwer Health, Inc. All Rights Reserved.

Bo C.,Chinese Academy of Sciences | Bo C.,Third Hospital of Wuhan | Ningbei Y.,Chinese Academy of Sciences
Journal of Craniofacial Surgery | Year: 2014

Background: Surgeons need references to undertake cleft lip repairs. We aimed to establish a three-dimensional model of upper lip muscles. Methods: We examined specimens from 2 adult cadaver heads and 8 adult cadaver lips, obtaining serial sections in the axial, sagittal, and coronal planes. Sections were stained to observe the philtrum, Cupid bow, vermilion, and nostril sill. Reconstruction was done with three-dimensional software (eg, 3D-DOCTOR, MicroMR). Results: Parallel circular muscle fibers existed between modioli. The orbicularis oris deep layer contained fan muscle fibers inclining inward. Some ended at the anterior nasal crest. Others migrated to the depressor septum, crossed the midline, and migrated to the nasalis muscle. At the nostril floor, the depressor septum muscle bundle and ipsilateral orbicularis oris overlapped the nasalis muscle and the contralateral orbicularis oris. This construction shaped the nostril sill. The levator labii superioris alaeque nasi, levator labii superioris, and zygomaticus minor crossed the nasolabial groove and migrated to the superficial orbicularis oris, entering the outer edge of the nasal alar to the upper lip near the vermilion border and philtrum ridge, shaping Cupid bow. Contralateral deep orbicularis oris muscle fibers crossed the philtrum dimple to the lateral philtrum ridge (axial plane). Superficial reticular muscle fibers of the levator labii superioris, zygomaticus minor, zygomaticus major, and orbicularis oris inserted into the medial philtrum ridge (coronal plane). They intersected to form the philtrum ridge. Conclusions: A three-dimensional upper lip muscular system model was established that can be referenced for cleft lip repair and lip operations. Copyright © 2014 by Mutaz B. Habal, MD.

Huang F.,Huazhong University of Science and Technology | Xiong X.,Third Hospital of Wuhan | Wang H.,Third Hospital of Wuhan | You S.,Huazhong University of Science and Technology | Zeng H.,Huazhong University of Science and Technology
Acta Biochimica et Biophysica Sinica | Year: 2010

Leptin is a peptide hormone primarily involved in the regulation of food intake and energy expenditure. Recent studies have suggested that leptin is one of the risk factors for cardiovascular diseases including atherosclerosis and hypertension. Vascular smooth muscle cells (VSMCs) play a vital role in arterial intimal thickening and vascular remodeling. In this study, we investigated the effect of leptin on VSMC cell-cycle regulation and the possible pathway. We found that leptin stimulated VSMC proliferation and increased cell progression to S and G2/M phases. The expression of cyclinD1, phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2), and nuclear factor (NF)-κBp65 was increased. Treatment of the cells with leptin antagonist triple mutant attenuated the leptininduced ERK1/2 and NF-κB activation. These results suggested that leptin stimulated VSMC proliferation by promoting transition from G1 to S phase and ERK1/2 and NF-κB pathway might contribute to this procession.

Wang X.,Third Hospital of Wuhan | Ma L.,Third Hospital of Wuhan | Wu J.-H.,Third Hospital of Wuhan
Pakistan Journal of Pharmaceutical Sciences | Year: 2014

Fructus Hordei Germinatus is widely used in treating hyperprolactinemia as a kind of Chinese traditional herb in China. However, its active composition of curing hyperprolactinemia remains unclear. This study investigates the activity of total alkaloids of F. H. Germinatus (AFH) in hyperprolactinemia rats. High-dose, middle-dose and low-dose AFH were administered into the stomach of hyperprolactinemia rats for 30 days. It revealed that high-dose AFH had obvious curative effect in treating hyperprolactinemia. It could regulate serum E2, P, PRL, FSH, LH levels to normal, decrease the pituitary prolactin positive cell number, mRNA expression level and inhibit the hyperplasia of mammary gland in hyperprolactinemia model rats effectively. The F. H. Germinatus contained total alkaloids 42. 74±0. 08mg hordenine equivalent (HE)/g the sample using acid dye colorimetry method. F. H. Germinatus should be developed as an anti-hyperprolactinemia product deeply.

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