Shijiazhuang, China
Shijiazhuang, China

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Zhang L.-M.,Capital Medical University | Ren L.,Third Hospital of Shijiazhuang | Zhao Z.-Q.,Third Hospital of Shijiazhuang | Zhao Y.-R.,Capital Medical University | And 2 more authors.
Medicine (United States) | Year: 2017

Rationale: Bacterial meningitis (BM) has been recognized as a rare complication of spinal surgery. However, there are few reports on the management of postoperative BM in patients who have undergone spinal surgery. The initial approach to the treatment of patients suspected with acute BM depends on the stage at which the syndrome is recognized, the speed of the diagnostic evaluation, and the need for antimicrobial and adjunctive therapy. Patient concerns: Here, we report the case of a patient with lumbar spinal stenosis and underwent a transforaminal lumbar interbody fusion at L4-L5. The dura mater was damaged intraoperatively. After the surgery, the patient displayed dizziness and vomiting. A CSF culture revealed Pseudomonas aeruginosa infection. Diagnoses: The patient was diagnosed with postoperative BM. Interventions: Antibiotic was administered intravenously depends on the organism isolated. Nevertheless, the patient's clinical condition continued to deteriorate. The patient underwent 2 open revision surgeries for dural lacerations and cyst debridement repair. Outcomes: The patient's mental status returned to normal and her headaches diminished. The patient did not have fever and the infection healed. Lessons: Surgical intervention is an effective method to treat BM after spinal operation in cases where conservative treatments have failed. Further, early surgical repair of dural lacerations and cyst debridement can be a treatment option for selected BM patients with complications including pseudomeningocele, wound infection, or cerebrospinal fluid leakage. © 2017 the Author(s). Published by Wolters Kluwer Health, Inc.


Gao F.,Hebei Medical University | Zhou X.-T.,Third Hospital of Shijiazhuang | Zhang Z.-F.,Hebei Medical University | Guo Y.,Hebei Medical University
Chinese Journal of Cancer Prevention and Treatment | Year: 2012

To investigate the relationship between single nucleotide polymorphisms of -460T/C in the promoter region of VEGF gene and the susceptibilitiy of lung cancer in Hebei province. This hospital-based case-control study included 200 lung cancer patients and 204 healthy controls. Genomic DNA was extracted by using proteinase K digestion followed by a salting out procedure. Polymorphisms of VEGF gene were analyzed by PCR-restriction fragment length polymorphism analysis (PCR-RFLP) and primer-introduced restriction analysis PCR (PIRA-PCR). The frequency of the VEGF-460 C/T SNP C allele in lung cancer patients (27.5%) was significantly higher than that in controls (20.1%, χ2=6.109, P=0.013). The frequencies of three genotypes (T/T and C/T+C/C) in controls were 63.2% and 36.7% respectively, and in lung cancer patients were 52.2% and 47.5% respectively. There was significant difference between the two groups (χ2=4.445, P=0.029).compared with individuals with the T/T genotype, individuals with the T/C+C/C genotype had significantly higher risk to develop lung cancer.compared with individuals with the T/T genotype, the T/C+C/C genotype increased the risk in developing lung cancer in non-smokers. When stratified by tumor histology, compared with individuals with the T/T genotype, individuals with the T/C+C/C genotype carried an increased risk of lung cancer in squamous cell carcinomas and small cell lung carcinomas. VEGF-460T/C SNP is associated with the risk of lung cancer.


Ji W.Q.,Hebei Medical University | Liu S.Y.,Hebei Medical University | Dai J.,Third Hospital of Shijiazhuang | Yang T.,Hebei Medical University | And 3 more authors.
Chinese Medical Journal | Year: 2014

Background Dyslipidemia caused by liver injury is a significant risk factor for cardiovascular complications. Previous studies have shown that hydrogen sulfide (H2S) protects against multiple cardiovascular disease states in a similar manner as nitric oxide (NO), and NO/endothelial nitric oxide synthase (eNOS) pathway is the key route of NO production. The purpose of this study was to investigate whether H2S can ameliorate the high blood pressure and plasma lipid profile in Nw-nitro-L-argininemethyl ester (L-NAME)-induced hypertensive rats by NO/eNOS pathway.Methods Thirty-six 4-week old Sprague-Dawley (SD) male rats were randomly assigned to 6 groups (n=6): control group, L-NAME group, control + glibenclamide group, control + NaHS group, L-NAME + NaHS group, and L-NAME + NaHS + glibenclamide group. Measurements were made of plasma triglycerides (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), total cholesterol (CHO), glutamic-pyruvic transaminase (ALT) levels after 5 weeks. Then measurements of NO level and proteins expression of eNOS, P-eNOS, AKT, P-AKT were made in liver tissue.Results After 5 weeks of L-NAME treatment, the blood pressure, plasma TG ((1.22±0.12) mmol/L in L-NAME group vs. (0.68±0.09) mmol/L in control group; P <0.05) and LDL ((0.54±0.04) mmol/L in L-NAME group vs. (0.28±0.02) mmol/L in control group; P <0.05) concentration were significantly increased, and the plasma HDL ((0.26±0.02) mmol/L in L-NAME group vs. (0.69±0.07) mmol/L in control group; P <0.05) concentration significantly decreased. Meanwhile the rats treated with L-NAME exhibit dysfunctional eNOS, diminished NO levels ((1.36±0.09) mmol/g protein in L-NAME group vs. (2.34±0.06) mmol/g protein in control group; P <0.05) and pathological changes of the liver. H2S therapy can markedly decrease the blood pressure ((37.25±4.46) mmHg at the fifth week; P <0.05), and ameliorate the plasma TG ((0.59±0.06) mmHg), LDL ((0.32±0.04) mmHg), and HDL ((0.46±0.03) mmHg) concentration in L-NAME + NaHS group (all P <0.05). H2S therapy can also restore eNOS function and NO bioavailability and attenuate the pathological changes in the liver in L-NAME-induced hypertensive rats.Conclusion H2S protects the L-NAME-induced hypertensive rats against liver injury via NO/ eNOS pathway, therefore decreases the cardiovascular risk. © 2014, Chinese Medical Association. All rights reserved.


Liu C.,Hebei Medical University | Zhou X.,Third Hospital of Shijiazhuang | Gao F.,Hebei Medical University | Qi Z.,Hebei Medical University | And 2 more authors.
Cancer Gene Therapy | Year: 2015

The aim of the study is to study the correlation of genetic polymorphism of vascular endothelial growth factor (VEGF) gene with susceptibility to primary lung cancer. A total of 414 patients with primary lung cancer and 338 healthy volunteers were enrolled in this case-control study from September 2008 to October 2011. Gene identification with PCR-RFLP (polymerase chain reaction-based restriction fragment length polymorphism) was used to detect in white blood cells from the subjects the single-nucleotide polymorphisms (SNP) of VEGF gene, including +405G/C, -460 T/C, -1154G/A, -2578C/A sites. Association of genotypes or haplotypes with susceptibility of lung cancer was analyzed with unconditional logistic regression adjusted by gender and age. Smoking was significantly associated with increased risk of lung cancer. Gene phenotypic analysis demonstrated that C allele of +405G/C in VEGF gene was significantly associated increased risk of lung cancer in males (P=0.0094, odds ratio=1.634.3), as that with carrying GCTC haplotype (odds ratio=1.349), whereas carrying GACG had decreased risk for lung cancer (odds ratio=0.044). No relationship existed between 460 T/C, -1154G/A, -2578C/A alleles of VEGF gene and risk of lung cancer. VEGF gene polymorphism may have a role in the development of lung cancer. © 2015 Nature America, Inc. All rights reserved.


Li T.,Hebei Medical University | Niu L.,Third Hospital of Shijiazhuang | Li M.,Hebei Medical University | Liu Y.,Hebei Medical University | And 3 more authors.
Molecular Medicine Reports | Year: 2015

The nuclear transcription factor Krüppel-like factor 4 (KLF4) has an important role in cellular biological processes. However, the influence of KLF4 on collagen metabolism remains to be elucidated. In the present study, the effects and underlying mechanism of action of KLF4 on collagen metabolism was investigated in human hepatic stellate cells (HSC), by downregulating KLF4 expression using small interfering RNA (siRNA). The effects of KLF4 silencing by three predesigned siRNAs (siRNA1-3) were evaluated using both reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting in the human LX2 HSC line. The mRNA expression levels of KLF4 were decreased by ∼34, 40, and 69% in the siRNA1, siRNA2, and siRNA3 groups, respectively, as compared with the control group. These results were concordant with the protein expression levels of KLF4, as determined by western blot analysis. In the siRNA3 group, the quantity of type I and type III collagen, and the expression levels of collagen metabolism proteins including matrix metalloproteinase-1 (MMP-1) and tissue inhibitors of metalloproteinases-1 (TIMP-1), were determined using both RT-qPCR and western blotting. Both the mRNA and protein expression levels of type I and type III collagen were significantly decreased in the siRNA3 group, as compared with the control group. The mRNA and protein expression levels of TIMP-1 were also significantly reduced in the siRNA3-treated cells, whereas the mRNA and protein expression levels of MMP-1 were significantly upregulated. Furthermore, KLF4 gene silencing significantly decreased the expression levels of numerous cytokines, including transforming grow factor-β1, tumor necrosis factor-α, and interleukin-1β. The results of the present study provide evidence of siRNA-mediated silencing of KLF4 expression, which may promote extracellular matrix (ECM) degradation, and inhibition of ECM synthesis. Therefore, KLF4 may be a promising target for the development of novel antifibrotic therapies.


PubMed | Third Hospital of Shijiazhuang and Hebei Medical University
Type: Journal Article | Journal: Chinese medical journal | Year: 2014

Dyslipidemia caused by liver injury is a significant risk factor for cardiovascular complications. Previous studies have shown that hydrogen sulfide (H2S) protects against multiple cardiovascular disease states in a similar manner as nitric oxide (NO), and NO/endothelial nitric oxide synthase (eNOS) pathway is the key route of NO production. The purpose of this study was to investigate whether H2S can ameliorate the high blood pressure and plasma lipid profile in Nw-nitro-L-argininemethyl ester (L-NAME)-induced hypertensive rats by NO/eNOS pathway.Thirty-six 4-week old Sprague-Dawley (SD) male rats were randomly assigned to 6 groups (n = 6): control group, L-NAME group, control + glibenclamide group, control + NaHS group, L-NAME + NaHS group, and L-NAME + NaHS + glibenclamide group. Measurements were made of plasma triglycerides (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), total cholesterol (CHO), glutamic-pyruvic transaminase (ALT) levels after 5 weeks. Then measurements of NO level and proteins expression of eNOS, P-eNOS, AKT, P-AKT were made in liver tissue.After 5 weeks of L-NAME treatment, the blood pressure, plasma TG ((1.220.12) mmol/L in L-NAME group vs. (0.680.09) mmol/L in control group; P < 0.05) and LDL ((0.540.04) mmol/L in L-NAME group vs. (0.280.02) mmol/L in control group; P < 0.05) concentration were significantly increased, and the plasma HDL ((0.260.02) mmol/L in L-NAME group vs. (0.690.07) mmol/L in control group; P < 0.05) concentration significantly decreased. Meanwhile the rats treated with L-NAME exhibit dysfunctional eNOS, diminished NO levels ((1.360.09) mmol/g protein in L-NAME group vs. (2.340.06) mmol/g protein in control group; P < 0.05) and pathological changes of the liver. H2S therapy can markedly decrease the blood pressure ((37.254.46) mmHg at the fifth week; P < 0.05), and ameliorate the plasma TG ((0.590.06) mmHg), LDL ((0.320.04) mmHg), and HDL ((0.460.03) mmHg) concentration in L-NAME + NaHS group (all P < 0.05). H2S therapy can also restore eNOS function and NO bioavailability and attenuate the pathological changes in the liver in L-NAME-induced hypertensive rats.H2S protects the L-NAME-induced hypertensive rats against liver injury via NO/ eNOS pathway, therefore decreases the cardiovascular risk.


PubMed | Third Hospital of Shijiazhuang, Dongying Peoples Hospital of Shandong Province and Hebei Medical University
Type: Journal Article | Journal: Surgical oncology | Year: 2016

This study was conducted to investigate the clinical significance of Slit2 and Robo1 expression in prognosis of patients with brain gliomas.Human brain tissue samples were collected from normal glial tissues (control), low- and high-grade glioma tissues. Slit2 and Robo1 expression levels in cells were assessed by an immunohistochemistry (IHC), and population of the Slit2- and Robo1-presenting patients was examined. The Slit2 and Robo1 mRNA expression levels in three types of the brain cells was determined by RT-PCR.Slit2Slit2 and Robo1 expression levels serve as a biomarker with utility in grading gliomas as well as predicting patient survival. The change in Slit2 expression is more reliable and effective than Robo1 expression in predicting a poor prognosis of brain glioma patients.


PubMed | Third Hospital of Shijiazhuang, Hebei Medical University and Hospital for Special Surgery
Type: | Journal: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA | Year: 2016

The present study was undertaken to evaluate the effect of tibial slope (TS) changes on the femorotibial articular contact kinematics in subjects undergoing posterior cruciate-retaining total knee arthroplasty (CRTKA).Eighteen knees in nine patients with medial osteoarthritis who underwent CRTKA using the same size prosthesis were analysed preoperatively and 2years after TKA. TS changes were calculated on lateral radiographs taken before and after TKA. Knees were classified into two groups according to the change in TS obtained by subtracting the post-operative value from the preoperative value: group 1 (>3) and group 2 (<3). The femorotibial articular contact kinematics of knees during weight-bearing flexion were compared between the two groups by two-dimensional/three-dimensional registration.Group 1 showed a continuous posterior translation of the medial femoral condyle during the process of knee flexion, whereas in group 2 the medial femoral condyle experienced paradoxical anterior motion from 20 to 90 of knee flexion. The lateral femoral condyle continuously moved posteriorly in both groups.A greater reduction in TS after TKA compared with preoperative TS reduces paradoxical medial femoral condylar movement. This may contribute to improved patient satisfaction after CR TKA.III.


PubMed | Fourth Hospital of Shijiazhuang, Hebei University of Science and Technology and Third Hospital of Shijiazhuang
Type: Journal Article | Journal: Advances in clinical and experimental medicine : official organ Wroclaw Medical University | Year: 2015

As the major neurotransmitter in the mammalian central nervous system (CNS), excessive extracellular glutamate (Glu) can activate the Glu receptors and neuronal calcium (Ca2+) overload, then produce neurotoxicity, which is a common pathway for neuronal injury or death, and is associated with acute and chronic neurodegenerative diseases. Therefore, it has been a therapeutic strategy to investigate neuroprotective effects against Glu-induced neurotoxicity for treating both acute and chronic forms of neurodegeneration. Resveratrol (Res), as a naturally occurring polyphenol mainly found in grapes and red wine, has shown a neuroprotective effect in a variety of experimental models for neurodegenerative diseases in vitro and in vivo. This review will focus on the neuroprotective effect of Res against Glu-induced excitotoxicity in neurodegenerative diseases by blocking different Glu receptors and Ca2+ ion channels.


Zhang L.,Third Hospital of Shijiazhuang | Song G.,Capital Medical University | Chen L.,Third Hospital of Shijiazhuang | Jiao L.,Capital Medical University | And 2 more authors.
Journal of Vascular Surgery | Year: 2016

The persistent primitive hypoglossal artery (PPHA) is a rare fetal variant of carotid-basilar anastomosis that increases the risk of ischemia and embolic infarction within the posterior cerebral circulation in patients with carotid stenosis proximal to the origin of the PPHA. A man presented with severe stenosis of the right internal carotid artery with extension to the origin of a PPHA. The area of stenosis was at a high position, which contraindicated carotid endarterectomy. Therefore, stenting was performed with proximal reversal of flow embolic protection. The unique anatomic and technical challenges associated with this case are reviewed in detail. Copyright © 2016 by the Society for Vascular Surgery. Published by Elsevier Inc.

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