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Mianyang, China

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Long X.,Chongqing Medical University | Wang F.,Tibet Autonomous Region in Chengdu Office Hospital | Huang C.-Q.,Third Hospital
Chinese Journal of Evidence-Based Medicine | Year: 2010

Objective: To assess the efficacy of Tongxinluo for diabetic kidney disease. Methods: we conducted a systematic review of randomized controlled trials (RCTs) in which Tongxinluo was used to treat diabetic kidney disease. And we screened relevant studies according to predefined inclusion and exclusion criteria, evaluated the quality of the included studies, and performed meta-analyses by using The Cochrane Collaboration's Revman 5.0 software. Results A total of 11 RCTs were enrolled in the review. The results of meta-analysis showed that Tongxinluo was better on attenuating 24 hour urinary protein,BUN and UAER; Tongxinluo was not superior to no treatment on the improvement of Scr and Ccr; Tongxinluo was better than no treatment on the Regulation of blood lipids, such as TC, TG, LDL-C. However, Tongxinluo might have similar effects on the improvement of HDL-C; Tongxinluo was better than no treatment on the improvement of FBG, but xuezhikang was not superior to no treatment on the improvement of P2BG and HbA1c. Tongxinluo was better than no treatment in decreasing plasma endothelin (ET). No significant adverse effects or Allergic reactions were reported. Conclusion: The evidence currently available shows that Tongxinluo has some effect and is relatively safe in treating patients with diabetic kidney disease.Due to a high risk of selection bias and detection bias in the included studies, the evidence is insufficient to determine the effect of Tongxinluo. Further large-scale trials are required to define the role of xuezhikang in the treatment of DKD. © 2010 Editorial Board of Chin J Evid-based Med.


Chen S.-D.,Third Hospital | Chen S.-D.,Peking University | Zhao R.-S.,Third Hospital
Chinese Journal of New Drugs | Year: 2016

Objective: To administrate the drug application for outpatients with chronic kidney disease (CKD) based on Chronic Disease Management Model, in order to improve the safety and effectiveness of drug therapy. Methods: For CKD patients who visited Peking University Third Hospital Renal Division from May 2015 to October 2015, clinical pharmacists together with the clinician in Department of Nephrology administrated patients' drug therapy, and filled their medication records including patient demographic information, disease diagnosis, laboratory parameters, and Medication Reconciliation list. Patients' medication-related problems (MRPs) were assessed in Chronic Disease Management Model guidance, and SPSS 21.0 was used for statistical analysis. Results: Totally 116 patients were included in the study. Sixty-two were male; mean age was (62±16.9) years (range 18~88 years). Forty-eight percent of the patients had stage 3 CKD, 29% stage 4, and 23% stage 5. Ninety-one patients (78%) had hypertension; 29 patients (25%) had diabetes mellitus; 62 patients (53%) had hyperuricemia. The average number of drugs used per patient was (4.5±2.6) species (range 1~13 species), and 59 patients (51%) took more than five species including drugs for hypertension, cardiovascular disease, hyperuricemia, renal anemia and renal bone disease. Sixty-five medication-related problems were found, involving drug safety, effectiveness, suitability, and compliance. Conclusion: Treatment of outpatients with chronic kidney disease is complicated, clinical pharmacist can administrate the drug application for outpatients effectively based on Chronic Disease Management Model. © 2016, Chinese Journal of New Drugs Co. Ltd. All right reserved.


Li H.,Third Hospital | Guo H.-Y.,Third Hospital | Han J.-S.,Third Hospital | Wang J.-L.,Peking University | And 3 more authors.
Journal of Minimally Invasive Gynecology | Year: 2011

Study Objective: To describe our experience with endoscopic removal of cesarean scar pregnancy. Design: Retrospective study (Canadian Task Force classification II-3). Setting: Tertiary-care university hospital. Patients: Twenty-one patients with cesarean scar pregnancy. Interventions: All the patients underwent removal of pregnancy mass at hysteroscopy or combined with laparoscopy. Nine patients received a methotrexate injection before the operation, and 13 underwent uterine artery embolization before surgery. Measurements and Main Results: Clinical data, serum β-human chorionic gonadotropin concentration, findings of ultrasound or magnetic resonance imaging examinations, therapeutic options, operative time, operative blood loss, and duration of hospitalization time were recorded. The mean serum β-human chorionic gonadotropin concentration at diagnosis was 53350.4 IU/ L. Seventeen patients underwent hysteroscopy, which failed in 2, and the other 4 patients underwent hysteroscopy combined with laparoscopy. Mean operative time was 51.4 minutes, and mean blood loss was estimated at 48.1 mL. A gestational mass can be removed at hysteroscopy, with rapid recovery and a high success rate. If a cesarean scar pregnancy mass grows toward the bladder and abdominal cavity, hysteroscopy combined with laparoscopy is more appropriate. Preoperative uterine artery embolization can decrease blood loss substantially during the operation. No patients underwent hysterectomy. Conclusions: Endoscopy seems to be the optimal surgical management in patients with a cesarean scar pregnancy and who desire to preserve the uterus and fertility. However, further study is warranted. © 2010 AAGL.


Pei Z.-H.,Third Hospital | Chen B.-Y.,PLA Fourth Military Medical University | Tie R.,PLA Fourth Military Medical University | Zhang H.-F.,PLA Fourth Military Medical University | And 5 more authors.
Cardiovascular Toxicology | Year: 2011

It has been reported that exposure to infrasound causes cardiac dysfunction. Allowing for the key role of apoptosis in the pathogenesis of cardiovascular diseases, the objective of this study was to investigate the apoptotic effects of infrasound. Cardiac myocytes cultured from neonatal rats were exposed to infrasound of 5 Hz at 130 dB. The apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling. Also, the expression levels of a series of apoptosis-related proteins were detected. As a result, infrasound induced apoptosis of cultured rat cardiac myocytes in a time-dependant manner. The expression of proapoptotic proteins such as Bax, caspase-3, caspase-8, caspase-9, and FAS was significantly up-regulated, with concomitant down-regulated expression of antiapoptotic proteins such as Bcl-x, and the inhibitory apoptosis proteins family proteins including XIAP, cIAP-1, and cIAP-2. The expression of poly (ADP-ribose) polymerase and β-catenin, which are the substrate proteins of caspase-3, was significantly decreased. In conclusion, infrasound is an apoptotic inducer of cardiac myocytes. © 2011 Springer Science+Business Media, LLC.


Wang L.,Huazhong University of Science and Technology | Wang L.,Third Hospital | Gong F.,Huazhong University of Science and Technology | Dong X.,Third Hospital | And 2 more authors.
Molecular and Cellular Biochemistry | Year: 2010

It has been reported that Nur77 over-expresses in arteriosclerotic lesions and has both pro- and anti-proliferative effects on vascular smooth muscle cells (VSMCs). We investigated the physiological function of Nur77 on proliferation in VSMCs and the effects of atorvastatin on the expression of Nur77. Platelet-derived growth factor (PDGF), a key growth factor mediating VSMC proliferation in atherogenesis and post-angioplasty restenosis, was employed to induce the transcriptional regulation of Nur77 expression in VSMCs and rat carotid artery post-angioplasty restenosis models were used to investigate the effect of atorvastatin on the expression of Nur77 by immunohistochemistry, RT-PCR, and western blot methods. In cell models, we found that PDGF-B induced Nur77 mRNA expression and protein expression through ERK-MAPK-dependent signaling pathways, and atorvastatin attenuated the expression of Nur77 induced by PDGF-B. In the rat model, our data showed Nur77 was up-regulated in neointima, but down-regulated by atorvastatin. Our results indicate that Nur77 promotes VSMC proliferation, and down-regulation of Nur77 by atorvastatin suggests a novel therapy strategy for atherogenesis based on suppression of VSMC proliferation. © 2010 Springer Science+Business Media, LLC.


Xie P.,Third Hospital | Huang J.-M.,Third Hospital | Lin H.-Y.,Third Hospital | Wu W.-J.,Third Hospital | Pan L.-P.,Third Hospital
International Urology and Nephrology | Year: 2013

Purpose: To compare the performance of the Chronic Kidney Disease Epidemiology Collaboration (CDK-EPI) equation, the 24-h creatinine clearance rate (24hCCr), Cockroft-Gault (C-G) formula, the abbreviated Modification of Diet in Renal Disease (aMDRD) equation, the modified Modification of Diet in Renal Disease (mMDRD) equation in determining glomerular filtration rate (GFR) in Chinese patients with chronic kidney disease (CKD) and detect the most proper method to measure GFR in clinical practice. Methods: One hundred and fifty-four patients with CKD were enrolled in the present study. 99mTc- diethylene triamine pentaacetic acid (99mTc-DTPA) plasma clearance method measured by dual plasma sampling method (rGFR) was considered as the reference standard. GFR was estimated simultaneously using five methods: (1) CDK-EPI equation (eGFR1); (2) 24hCCr (eGFR2); (3) C-G formula (eGFR3); (4) abbreviated MDRD equation (eGFR4); (5) mMDRD equation (eGFR5). The comparison of correlation, regression, bias, precision, accuracy, limit of agreement, and receiver-operating characteristics (ROC) for detecting CKD (with a GFR cutoff of 60 mL min-1 1.73 m-2) among the methods was analyzed to identify the most suitable method. Results: All the equations correlated well with rGFR, and the correlation coefficient of CDK-EPI equation was the highest (reGFR1 = 0.922, P < 0.001). The Bland-Altman analysis showed that the limit of agreement for CDK-EPI equation was -25.5 to 30.3 mL min -1 1.73 m-2, which was the least range among the tested equations. The CDK-EPI equation represented the best capability in precision (14.24 mL min-1 1.73 m-2). The ROC curve showed the best performance in detecting CKD. The accuracy within ±30 % of CDK-EPI equation, 24hCCr, and mMDRD equation was 72.08, 69.48, and 70.13 %, respectively, and no statistical significant difference was found (P > 0.05). However, CDK-EPI equation had the highest accuracy when compared with the other two equations (P < 0.05). And its performance on bias showed no statistically significant difference compared with other four equations. Conclusions: Although its bias and accuracy did not overmatch the other four equations in our patient group, the CDK-EPI equation outperformed the other equations based on creatinine in correlation, precision, limit of agreement, and detecting CKD, and it is very simple, time-saving, and cost-effective. So we recommend intensely that the CDK-EPI equation is the most suitable method in determining GFR in Chinese patients with CKD and can be applied generally in clinical practice. © 2012 Springer Science+Business Media Dordrecht.


Jia J.,Fudan University | Zhang F.,Fudan University | Hu Y.-S.,Fudan University | Wu Y.,Fudan University | And 7 more authors.
Experimental Brain Research | Year: 2010

This study was designed to investigate the neuroprotective eVect of treadmill pre-training against the over-release of glutamate resulting from cerebral ischemia. Sprague-Dawley rats underwent 2 weeks of treadmill runtraining before cerebral ischemia was performed by middle cerebral artery occlusion. The level of glutamate in brain extracellular Xuid was detected before, during and after ischemia/reperfusion. The expression of metabotropic glutamate receptor-1 (mGluR1) mRNA in striatum was examined after ischemia for 80 min and reperfusion for 240 min. Neurological defect score and brain infarction volumes were measured. The treadmill pre-training signiWcantly suppressed the release of glutamate, and reduced the expression of mGluR1 mRNA at 59% (P < 0.01) and 62% (P < 0.05), respectively, when compared with the ischemia group. The neurological defect score and infarction volume were signiWcantly improved by 75% (P < 0.01) and 74% (P < 0.01), respectively, in the pre-training group, when compared to the ischemia group. Treadmill pre-training has a signiWcant neuroprotective function against ischemia/ reperfusion injury, by suppressing glutamate release resulting from cerebral ischemia, and this eVect may be mediated by downregulation of mGluR1. © 2010 Springer-Verlag.


Zhao X.-F.,Northwest University, China | Huang J.-J.,Northwest University, China | Li Q.,Northwest University, China | Wei L.-S.,Northwest University, China | And 4 more authors.
Journal of Molecular Recognition | Year: 2013

The development of new approaches to study the affinity between ligands and G-protein-coupled receptors proves to be of growing interest for pharmacologists, chemists, and biologists. The aim of this work was to determine the binding of seven drugs to β2-adrenoceptors by frontal analysis using immobilized receptor stationary phase. The dissociation constants (Kd) were determined to be (3.16 ± 0.09) × 10 -4 M for salbutamol, (4.29 ± 0.12) × 10-4 M for terbutaline, (6.19 ± 0.16) × 10-4 M for methoxyphenamine, (2.11 ± 0.07) × 10-4 M for tulobuterol, (1.82 ± 0.11) × 10-4 M for fenoterol, (9.75 ± 0.24) × 10-6 M formoterol, and (9.84 ± 0.26) × 10-5 M for clenbuterol. These results showed a good correlation with the data determined by radioligand binding assay. Further investigations revealed that the dissociation constant mainly attributed to the number of hydrogen bonds in the structures of ligands. This study indicates that affinity chromatography using immobilized receptor stationary phase can be used for the direct determination of drug-receptor binding interactions and has the potential to become a reliable alternative for quantitative studies of ligand-receptor interactions. Copyright © 2013 John Wiley & Sons, Ltd.


PubMed | Third Hospital
Type: Journal Article | Journal: Pathology, research and practice | Year: 2012

The present study was designed to investigate the clinicopathological correlation between the expression of KLF5 and MMP-9, which are associated with extracellular matrix degradation and cartilage degeneration in human knee osteoarthritis (OA). Tibiofemoral joint samples from 20 patients with OA, treated with surgery alone, were divided into two groups: 0=no change (NC, n=17), and severe changes with a higher mean score ( 3) (SC, n=29). The latter group contains samples with severe damages in cartilages and subchondral bones at medial tibial plateaux. The expression of the proteins was detected by immunofluorescence and quantitative RT-PCR, respectively. Neurovascular invasion was evaluated by protein gene product (PGP) 9.5 and CD34-positive staining and scanning electron microscopy, respectively. Safranin O staining showed that the sections from the SC group had increased cartilage degeneration. The number of vascular invasions in the SC group (16/29, 55.2%) was higher than that in NC controls (2/17, 11.7%, P<0.05). The expression of KLF5 and MMP-9 increased, and was co-localized in the same cells of SC cartilages. The severity of cartilage degeneration and vascular invasion was associated with upregulation of the two protein expressions and was significantly different between SC and NC samples (P<0.05). Taken together, the expression of KLF5 and MMP-9 may be involved in cartilage degeneration, contributing to human OA.


PubMed | Third Hospital
Type: Journal Article | Journal: Cardiovascular toxicology | Year: 2011

It has been reported that exposure to infrasound causes cardiac dysfunction. Allowing for the key role of apoptosis in the pathogenesis of cardiovascular diseases, the objective of this study was to investigate the apoptotic effects of infrasound. Cardiac myocytes cultured from neonatal rats were exposed to infrasound of 5Hz at 130dB. The apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling. Also, the expression levels of a series of apoptosis-related proteins were detected. As a result, infrasound induced apoptosis of cultured rat cardiac myocytes in a time-dependant manner. The expression of proapoptotic proteins such as Bax, caspase-3, caspase-8, caspase-9, and FAS was significantly up-regulated, with concomitant down-regulated expression of antiapoptotic proteins such as Bcl-x, and the inhibitory apoptosis proteins family proteins including XIAP, cIAP-1, and cIAP-2. The expression of poly (ADP-ribose) polymerase and -catenin, which are the substrate proteins of caspase-3, was significantly decreased. In conclusion, infrasound is an apoptotic inducer of cardiac myocytes.

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