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Kang L.-H.,Chongqing Medical University | Liu M.-J.,Chongqing Medical University | Xu W.-C.,Chongqing Medical University | Cui J.-J.,Chongqing Medical University | And 4 more authors.
Saudi Medical Journal

Objectives: To investigate the molecular epidemiology of pneumococcal isolates in Chongqing, China. Methods: In this cross-sectional study, 51 invasive Streptococcus pneumoniae (S. pneumoniae) strains were from children with invasive pneumococcal disease (IPD) and 32 carriage strains from healthy children from January 2010 to December 2013 at the Children’s Hospital of Chongqing Medical University, Chongqing, China. Multilocus sequence typing was used to identify the sequence types (STs). Capsular serotypes were determined by multiplex polymerase chain reaction. Drug susceptibility and resistance was determined by minimum inhibitory concentrations. Results: In this study, 11 serotypes were identified among the 83 S. pneumoniae clinical isolates tested. Prevalent serotypes were 19A (20.4%), 6A/B (20.4%), 19F (15.7%), 14 (14.5%), and 23F (10.8%). Serotype 19F was the most frequent carriage strain, and serotype 19A was the most frequent invasive strain. The ST983 was the most prevalent ST for carriage strains, and ST320 was the most prevalent ST for invasive strains. For gene analysis, psaA (99.5%) and piaA (98.6%) were present and much conserved in all pneumococci tested. The cps2A and pcsB genes were more frequent in invasive isolates than carriage strains. Antimicrobial resistance rates of invasive pneumococcal isolates to erythromycin, penicillin, meropenem, cefotaxime, and clindamycin were higher than the carriage isolates from children. Conclusion: Our epidemiological evidence shows that 19A, 6A/B, 19F, 14, and 23F remain the most prevalent serotypes, which can be targeted by PCV13. Genotypes and drug resistance varied between carriage and invasive strains. The PsaA and PiaA may be good protein vaccine candidates. © 2016, Saudi Arabian Armed Forces Hospital. All rights reserved. Source

Zhu Q.N.,Zunyi Medical College | Xie H.M.,Third Affiliated Hospital of Zunyi Medical College | Zhang D.,Zunyi Medical College | Liu J.,Zunyi Medical College | And 2 more authors.

Background. Significant physiological changes occur during pregnancy and lactation. Intrahepatic cholestasis of pregnancy (ICP) is a liver disease closely related to disruption of bile acid homeostasis. The objective of this study was to examine the regulation of bile acid synthesis and transport in normal pregnant and lactating rats. Materials andMethods. Livers from timed pregnant SD rats were collected on gestational days (GD) 10, 14 and 19, and postnatal days (PND) 1, 7, 14 and 21. Total bile acids were determined by the enzymatic method, total RNA was isolated and subjected to real time RT-PCR analysis. Liver protein was extracted for western-blot analysis. Results. Under physiological conditions hepatic bile acids were not elevated during pregnancy but increased during lactation in rats. Bile acid synthesis rate-limiting enzyme Cyp7a1 was unchanged on gestational days, but increased on PND14 and 21 at mRNA and protein levels. Expression of Cyp8b1, Cyp27a1 and Cyp7b1 was also higher during lactation. The mRNA levels of small heterodimer partner (SHP) and protein levels of farnesoid X receptor (FXR) were increased during pregnancy and lactation. Bile acid transporters Ntcp, Bsep, Mrp3 and Mrp4 were lower at gestation, but increased during lactation. Hepatic Oatp transporters were decreased during pregnancy and lactation. Conclusion. Hepatic bile acid homeostasis is maintained during normal pregnancy in rats, probably through the FXR-SHP regulation. The expression of bile acid synthesis genes and liver bile acid accumulation were increased during lactation, together with increased expression of bile acid efflux transporter Bsep, Mrp3 and Mrp4. © 2013 Zhu et al. Source

Yang X.-Z.,Third Affiliated Hospital of Zunyi Medical College | Chen L.-Y.,Third Affiliated Hospital of Zunyi Medical College
World Chinese Journal of Digestology

AIM: To explore the effect of laparoscopic-assisted surgery for perforated peptic ulcer to provide evidence for the clinical treatment of such disease. METHODS: One hundred and six patients with perforated peptic ulcer were enrolled in this study from February 2010 to October 2013 at our hospital. The patients were randomly assigned to receive traditional laparotomy (control group) or laparoscopic-assisted surgery (treatment group), with 53 patients in each group. The operative time, blood loss, flatus time and other perioperative indicators, as well as pain score, painkiller utilization and complication rate were recorded and compared between the two groups. RESULTS: The treatment group had significantly lower postoperative blood loss, ambulation time, flatus time, hospitalization time and time to recovery of bowel sounds than the control group (42.0 mL ± 12.1 mL vs 156.0 mL ± 34.6 mL, 10.4 h ± 2.9 h vs 38.7 h ± 9.7 h, 16.8 h ± 2.3 h vs 40.6 h ± 6.9 h, 6.6 d ± 2.1 d vs 12.8 d ± 3.5 d, 18.4 h ± 5.6 h vs 45.6 h ± 7.7 h, P < 0.05). The pain scores on postoperative days 1, 2 and 7 were significantly lower in the treatment group than in the control group (3.1 ± 0.4 vs 5.3 ± 0.3, 2.4 ± 0.6 vs 4.4 ± 0.4, 1.6 ± 0.2 vs 3.2 ± 0.3, 11.3% vs 43.4%, P < 0.05). The incidences of wound infection and inflammation were significantly lower in the treatment group than in the control group (P < 0.05). CONCLUSION: The effect of laparoscopic-assisted surgery is superior to that of traditional laparotomy in the management of perforated peptic ulcer. © 2014 Baishideng Publishing Group Co., Limited. All rights reserved. Source

Deng J.,Third Affiliated Hospital of Zunyi Medical College | She R.F.,Guizhou Provincial Peoples Hospital | Huang W.L.,Third Affiliated Hospital of Zunyi Medical College | Yuan C.,Third Affiliated Hospital of Zunyi Medical College | Mo G.,Third Affiliated Hospital of Zunyi Medical College
Genetics and Molecular Research

A previous experiment demonstrated that fibroin protein and chitosan mixed in proper proportion presented good physical and chemical properties and biological characteristics, which can make up for their respective disadvantages. To observe the growth of bone marrow mesenchymal stem cells (BMSCs) on these fibroin protein/ chitosan 3D scaffolds, induced rabbit BMSCs were seeded on fibroin protein/chitosan scaffolds. The cell adhesion rate was measured, and cell growth was observed under an inverted microscope and a scanning electron microscope. The cell adhesion rate increased with time. The inverted microscope observations showed that the cells on fibroin protein/chitosan scaffolds could not be seen clearly. As time passed, the number of cells around the stent increased and some cells stretched inside the scaffolds. Electron microscopy showed active cell growth and normal proliferation, and the granular and filamentous matrix substances could be seen around cells. The microfilaments of cell and scaffold materials were tightly connected. The cells not only grew on the surface of the adherent material, but also stretched inside of the materials. These results indicated that the fibroin protein/ chitosan mixed scaffolds have good biocompatibility. © FUNPEC-RP www.funpecrp.com.br. Source

Deng J.,Third Affiliated Hospital of Zunyi Medical College | She R.,Guizhou Provincial Peoples Hospital | Huang W.,Third Affiliated Hospital of Zunyi Medical College | Dong Z.,Third Affiliated Hospital of Zunyi Medical College | And 2 more authors.
Journal of Materials Science: Materials in Medicine

Bone marrow-derived mesenchymal stem cells (BMSCs) were seeded in a three-dimensional scaffold of silk fibroin (SF) and chitosan (CS) to repair cartilage defects in the rabbit knee. Totally 54 rabbits were randomly assigned to BMSCs + SF/CS scaffold, SF/CS scaffold and control groups. A cylindrical defect was created at the patellofemoral facet of the right knee of each rabbit and repaired by scaffold respectively. Samples were prepared at 4, 8 and 12 weeks post-surgery for gross observation, hematoxylin-eosin and toluidine blue staining, type II collagen immunohistochemistry, Wakitani histology. The results showed that differentiated BMSCs proliferated well in the scaffold. In the BMSCs + SF/CS scaffold group, the bone defect was nearly repaired, the scaffold was absorbed and immunohistochemistry was positive. In the SF/CS scaffold alone group, fiber-like tissues were observed, the scaffold was nearly degraded and immunohistochemistry was weakly positive. In the control group, the defect was not well repaired and positive immunoreactions were not detected. Modified Wakitani scores were superior in the BMSCs + SF/CS scaffold group compared with those in other groups at 4, 8 and 12 weeks (P < 0.05). A SF/CS scaffold can serve as carrier for stem cells to repair cartilage defects and may be used for cartilage tissue engineering. © 2013 Springer Science+Business Media New York. Source

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