Third Affiliated Hospital of Xinxiang Medical College

Xinxiang, China

Third Affiliated Hospital of Xinxiang Medical College

Xinxiang, China
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Tan Z.,General Hospital of Air Force | Zhang Y.,General Hospital of Air Force | Chen W.,General Hospital of Air Force | Gong W.,General Hospital of Air Force | And 2 more authors.
American Journal of Otolaryngology - Head and Neck Medicine and Surgery | Year: 2015

Objective To study the role of total facial nerve decompression in preventing further recurrence of facial palsy in Melkersson Rosenthal syndrome (MRS). Methods Total facial nerve decompression was performed on nine patients with recurrent facial palsy in MRS, and prednisolone treatment was given to 6 cases who declined surgery. They were incorporated into surgery group and control group, respectively. Patients in surgery group and control group were followed up for 5.4 ± 1.4 years (range, 4 to 8 years) and 6.0 ± 1.4 years (range, 4 to 8 years), respectively. Results Further episodes of facial palsy affected none of 9 cases (0.0%) in surgery group, while they affected 3 of 6 cases (50.0%) in control group, with significant difference (p < 0.05). Conclusions Total facial nerve decompression was effective to prevent further episodes of facial palsy in MRS. © 2015 Published by Elsevier Inc.


Chen J.,Guilin Medical University | Duan Y.,Third Affiliated Hospital of Xinxiang Medical College | Zhang X.,Guilin Medical University | Ye Y.,Guangxi Medical University | Ge B.,Guilin Medical University
Food and Function | Year: 2015

Genistein is an estrogenic soy-derived compound belonging to the isoflavone class and shows anti-cancer effects. However, the specific cell apoptosis mechanisms of genistein have not been fully understood. In this study, we investigated the specific cell apoptosis mechanisms of genistein and the potential involvement of the IGF1R-Akt-Bcl-2 and Bax-mediated pathways in human breast cancer cells in vitro. MCF-7 human breast cancer cells were treated with various concentrations of genistein, and cell proliferation was evaluated by the MTT assay. Morphological changes in treated cells were examined by Hoechst 33258 staining, and treated cells were examined by flow cytometry. The levels of IGF-1R, p-Akt, Bcl-2, and Bax protein expression and Bcl-2 and Bax mRNA expression were evaluated by western blot and RT-PCR, respectively. Genistein inhibited the proliferation of MCF-7 cells and induced cell apoptosis, as determined by Hoechst staining and flow cytometry analysis. Furthermore, genistein induced the inactivation of IGF-1R and p-Akt and downregulated the Bcl-2/Bax protein ratio. These results suggest that genistein inhibited cell proliferation by inactivating the IGF-1R-PI3 K/Akt pathway and decreasing the Bcl-2/Bax mRNA and protein expressions. Our findings help elucidate the mechanisms by which genistein may contribute to the prevention of breast cancer carcinogenesis. This journal is © The Royal Society of Chemistry 2015.


Tian J.,Guilin Medical University | Duan Y.X.,Third Affiliated Hospital of Xinxiang Medical College | Bei C.Y.,Guilin Medical University | Chen J.,Guilin Medical University
Hormone and Metabolic Research | Year: 2013

Breast cancer is a leading cause of cancer death among women, and the failure of normal apoptosis has been proved in the development of breast cancer. The phytoestrogen, calycosin, is extracted from Chinese medical herb Radix astragali. We recently reported that calycosin successfully stimulated proliferation of ER-positive MCF-7 human breast cancer cells at low concentration. In the present study, we assessed the proapoptotic function of calycosin in MCF-7 cells at high concentration in vitro, as well as the possible mechanism of its effect. MCF-7 cells were treated with different concentrations of calycosin, and then detected by MTT assay for cellular viability, Hoechst assay, and flow cytometry for apoptosis. RASD1 is identified as a Ras-family member and a regulator in MAPK-mediated cascade leading to cell proliferation or apoptosis. To provide insight into the functions of RASD1 signaling pathway in calycosin-induced apoptosis, the expression of Bcl-2, Bax, and RASD1 in calycosin-treated cells were determined by Western blot assay. The results showed that high concentrations of calycosin significantly suppressed the proliferation of MCF-7 cells and promoted cell apoptosis. Moreover, compared with control group, the expression of Bcl-2 decreased with calycosin in MCF-7 cells, while Bax increased, which was significantly correlated with elevated expression of RASD1. Together, we present evidence that at relatively high concentration calycosin triggered cell apoptosis through the mitochondrial apoptotic pathway by upregulating RASD1. And for the first time, this study revealed that calycosin may have potential as a therapeutic agent for the treatment of breast cancer. © Georg Thieme Verlag KGStuttgart. New York.


Li W.L.,Xinxiang Medical University | Wang L.L.,Third Affiliated Hospital of Xinxiang Medical College | Luo Q.Y.,Third Affiliated Hospital of Xinxiang Medical College
The Scientific World Journal | Year: 2013

To develop a new facile protocol for the synthesis of 2′- aminobenzothiazolo-arylmethyl-2-naphthol derivatives, N-bromosuccinimide (NBS) was used as an efficient catalyst for the one-pot synthesis of 2′-aminobenzothiazolo-arylmethyl-2-naphthols in excellent yields from β-naphthol (1 mmol), aromatic aldehydes (1 mmol), and 2-aminobenzothiazole (1 mmol) at 60°C under solvent-free conditions. © 2013 Wei Lin Li et al.


PubMed | North Sichuan Medical College and Third Affiliated Hospital of Xinxiang Medical College
Type: Journal Article | Journal: PloS one | Year: 2013

To explore spleen hemodynamic alteration in liver fibrosis with dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), and to determine how to stage liver fibrosis with spleen DCE-MRI parameters.Sixteen piglets were prospectively used to model liver fibrosis staged by liver biopsy, and underwent spleen DCE-MRI on 0, 5th, 9th, 16th and 21st weekend after modeling this disease. DCE-MRI parameters including time to peak (TTP), positive enhancement integral (PEI), maximum slope of increase (MSI) and maximum slope of decrease (MSD) of spleen were measured, and statistically analyzed to stage this disease.Spearmans rank correlation tests showed that TTP tended to increase with increasing stages of liver fibrosis (r=0.647, P<0.001), and that PEI tended to decrease from stage 0 to 4 (r=-0.709, P<0.001). MSD increased slightly from stage 0 to 2 (P>0.05), and decreased from stage 2 to 4 (P<0.05). MSI increased from stage 0 to 1, and decreased from stage 1 to 4 (all P>0.05). Mann-Whitney tests demonstrated that TTP and PEI could classify fibrosis between stage 0 and 1-4, between 0-1 and 2-4, between 0-2 and 3-4, or between 0-3 and 4 (all P<0.01). MSD could discriminate between 0-2 and 3-4 (P=0.006), or between 0-3 and 4 (P=0.012). MSI could not differentiate between any two stages. Receiver operating characteristic analysis illustrated that area under receiver operating characteristic curve (AUC) of TTP was larger than of PEI for classifying stage 1 and 2 (AUC=0.851 and 0.783, respectively). PEI could best classify stage 3 and 4 (AUC=0.903 and 0.96, respectively).Spleen DCE-MRI has potential to monitor spleen hemodynamic alteration and classify liver fibrosis stages.


Wang F.,Xinxiang Central Hospital | Liu S.Y.,Third Affiliated Hospital of Xinxiang Medical College | Du T.P.,Xinxiang Central Hospital | Chen H.,Xinxiang Central Hospital | And 2 more authors.
Experimental and Therapeutic Medicine | Year: 2014

An effective treatment for hepatic fibrosis is not available clinically. Nuclear factor (NF)-κB plays a central role in inflammation and fibrosis. The aim of the present study was to investigate the effect of an NF-κB inhibitor, BAY-11-7082 (BAY), on mouse liver fibrosis. The effects of BAY on hepatic stellate cell (HSC) activation were measured in the lipopolysaccharide-activated rat HSC-T6 cell line. In addition, the therapeutic effect of BAY was studied in vivo using a model of hepatic fibrosis induced by carbon tetrachloride (CCl4) in mice. BAY effectively decreased the cell viability of activated HSC-T6 cells and suppressed HSC-T6 activation by downregulating the expression of collagen I and α-smooth muscle actin. BAY significantly inhibited the phosphorylation of phosphatidylinositol 3-kinase (PI3K) and serine/threonine kinase-protein kinase B (Akt) in activated HSC-T6 cells. In addition, administration of BAY attenuated mouse liver fibrosis induced by CCl4, as shown by histology and the expression of profibrogenic markers. BAY also significantly decreased the levels of serum alanine aminotransferase in this model of hepatic fibrosis. Therefore, the results of the present study demonstrate that BAY attenuates liver fibrosis by blocking PI3K and Akt phosphorylation in activated HSCs. Thus, BAY demonstrates therapeutic potential as a treatment for hepatic fibrosis.


Yang W.,Third Affiliated Hospital of Xinxiang Medical College | Zhao J.,Third Affiliated Hospital of Xinxiang Medical College | Han Y.,Third Affiliated Hospital of Xinxiang Medical College | Yang H.,Third Affiliated Hospital of Xinxiang Medical College | And 4 more authors.
American Journal of Otolaryngology - Head and Neck Medicine and Surgery | Year: 2015

Objective The study aimed to report long-term outcomes of facial nerve schwannomas (FNS) with favorable facial nerve function by observation, and to discuss about the relationship between initial tumor size and tumor growth. Methods 21 facial nerve schwannoma cases with favorable facial nerve function were managed by observation. They were divided into larger size group (size ≥10 mm) and smaller size group (size < 10 mm) according to initial tumor size. Results They were followed up for 6.4 ± 1.7 years. 18 of 21 cases (85.7%) maintained House-Brackmann Grade III or better. Growth rate of the tumors in larger size group was 72.7%, much higher than 10% in smaller size group (p < 0.05). Conclusions Observation was feasible for most FNS with favorable facial nerve function, and growth rate of the tumors was associated with tumor size. Copyright © 2015 Published by Elsevier Inc. All rights reserved.


PubMed | Zhengzhou University and Third Affiliated Hospital of Xinxiang Medical College
Type: Journal Article | Journal: Oncotarget | Year: 2016

Adoptive immunotherapy using cytokine-induced killer (CIK) cells is a promising cancer treatment, but its efficacy is restricted by various factors, including the accumulation of myeloid-derived suppressor cells (MDSCs). In this study, we determine whether chemotherapeutic drugs that reduce MDSC levels enhance the efficacy of CIK cell therapy in the treatment of solid tumors. Fifty-three patients were included in this study; 17 were diagnosed with metastatic renal cell carcinoma (MRCC), 10 with advanced pancreatic cancer (PC), and 26 with metastatic melanoma (MM). These patients were divided into two groups: CIK cell therapy alone and CIK cell therapy combined with chemotherapy. Combining CIK cell therapy and chemotherapy increased 1-year survival rates and median survival times in MRCC and PC patients, but not in MM patients. The disease control rate did not differ between treatment groups for MRCC or MM patients, but was higher in PC patients receiving combined treatment than CIK cell treatment alone. These data suggest that addition of MDSC-decreasing chemotherapy to CIK cell therapy improves survival in MRCC and PC patients.


PubMed | Zhengzhou University and Third Affiliated Hospital of Xinxiang Medical College
Type: | Journal: Journal of hematology & oncology | Year: 2016

Advanced pancreatic cancer (PC) has very poor prognosis with present treatments, thus necessitating continued efforts to find improved therapeutic approaches. Both preclinical and preliminary clinical data indicate that cytokine-induced killer (CIK) cells are an effective tool against various types of solid tumors. Here, we conducted a study to determine whether CIK cell-based therapy (CBT) can improve the outcomes of advanced PC.Eighty-two patients with advanced PC, whose predicted survival time was longer than 3 months, were analyzed retrospectively. Of all the patients, 57 individuals were receiving chemotherapy, while the remaining 25 individuals were treated with CBT.The overall survival analysis was based on 48 deaths in the 57 patients in the chemotherapy group (84.2%) and 18 deaths in the 25 patients in the CBT group (72.0%). In the CBT group, the median overall survival time was 13.5 months, as compared to 6.6 months in the chemotherapy group (hazard ratio for death, 0.39; 95% confidence interval, 0.23 to 0.65; p<0.001). The survival rate was 88.9% in the CBT group versus 54.2% in the chemotherapy group at 6 months, 61.1% versus 12.5% at 12 months, and 38.9% versus 4.2% at 18 months. The disease control rate was 68.0% in the CBT group and 29.8% in the chemotherapy group (p<0.001).These results from this retrospective analysis appeared to imply that CBT might prolong survival in these high-risk PC patients. Prospective study is needed to corroborate this observation.


PubMed | Third Affiliated Hospital of Xinxiang Medical College
Type: Comparative Study | Journal: American journal of otolaryngology | Year: 2015

The study aimed to report long-term outcomes of facial nerve schwannomas (FNS) with favorable facial nerve function by observation, and to discuss about the relationship between initial tumor size and tumor growth.21 facial nerve schwannoma cases with favorable facial nerve function were managed by observation. They were divided into larger size group (size 10mm) and smaller size group (size <10mm) according to initial tumor size.They were followed up for 6.41.7years. 18 of 21 cases (85.7%) maintained House-Brackmann Grade III or better. Growth rate of the tumors in larger size group was 72.7%, much higher than 10% in smaller size group (p<0.05).Observation was feasible for most FNS with favorable facial nerve function, and growth rate of the tumors was associated with tumor size.

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