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Chen J.,Guilin Medical University | Duan Y.,Third Affiliated Hospital of Xinxiang Medical College | Zhang X.,Guilin Medical University | Ye Y.,Guangxi Medical University | Ge B.,Guilin Medical University
Food and Function | Year: 2015

Genistein is an estrogenic soy-derived compound belonging to the isoflavone class and shows anti-cancer effects. However, the specific cell apoptosis mechanisms of genistein have not been fully understood. In this study, we investigated the specific cell apoptosis mechanisms of genistein and the potential involvement of the IGF1R-Akt-Bcl-2 and Bax-mediated pathways in human breast cancer cells in vitro. MCF-7 human breast cancer cells were treated with various concentrations of genistein, and cell proliferation was evaluated by the MTT assay. Morphological changes in treated cells were examined by Hoechst 33258 staining, and treated cells were examined by flow cytometry. The levels of IGF-1R, p-Akt, Bcl-2, and Bax protein expression and Bcl-2 and Bax mRNA expression were evaluated by western blot and RT-PCR, respectively. Genistein inhibited the proliferation of MCF-7 cells and induced cell apoptosis, as determined by Hoechst staining and flow cytometry analysis. Furthermore, genistein induced the inactivation of IGF-1R and p-Akt and downregulated the Bcl-2/Bax protein ratio. These results suggest that genistein inhibited cell proliferation by inactivating the IGF-1R-PI3 K/Akt pathway and decreasing the Bcl-2/Bax mRNA and protein expressions. Our findings help elucidate the mechanisms by which genistein may contribute to the prevention of breast cancer carcinogenesis. This journal is © The Royal Society of Chemistry 2015. Source


Tian J.,Guilin Medical University | Duan Y.X.,Third Affiliated Hospital of Xinxiang Medical College | Bei C.Y.,Guilin Medical University | Chen J.,Guilin Medical University
Hormone and Metabolic Research | Year: 2013

Breast cancer is a leading cause of cancer death among women, and the failure of normal apoptosis has been proved in the development of breast cancer. The phytoestrogen, calycosin, is extracted from Chinese medical herb Radix astragali. We recently reported that calycosin successfully stimulated proliferation of ER-positive MCF-7 human breast cancer cells at low concentration. In the present study, we assessed the proapoptotic function of calycosin in MCF-7 cells at high concentration in vitro, as well as the possible mechanism of its effect. MCF-7 cells were treated with different concentrations of calycosin, and then detected by MTT assay for cellular viability, Hoechst assay, and flow cytometry for apoptosis. RASD1 is identified as a Ras-family member and a regulator in MAPK-mediated cascade leading to cell proliferation or apoptosis. To provide insight into the functions of RASD1 signaling pathway in calycosin-induced apoptosis, the expression of Bcl-2, Bax, and RASD1 in calycosin-treated cells were determined by Western blot assay. The results showed that high concentrations of calycosin significantly suppressed the proliferation of MCF-7 cells and promoted cell apoptosis. Moreover, compared with control group, the expression of Bcl-2 decreased with calycosin in MCF-7 cells, while Bax increased, which was significantly correlated with elevated expression of RASD1. Together, we present evidence that at relatively high concentration calycosin triggered cell apoptosis through the mitochondrial apoptotic pathway by upregulating RASD1. And for the first time, this study revealed that calycosin may have potential as a therapeutic agent for the treatment of breast cancer. © Georg Thieme Verlag KGStuttgart. New York. Source


Tan Z.,General Hospital of Air Force | Zhang Y.,General Hospital of Air Force | Chen W.,General Hospital of Air Force | Gong W.,General Hospital of Air Force | And 2 more authors.
American Journal of Otolaryngology - Head and Neck Medicine and Surgery | Year: 2015

Objective To study the role of total facial nerve decompression in preventing further recurrence of facial palsy in Melkersson Rosenthal syndrome (MRS). Methods Total facial nerve decompression was performed on nine patients with recurrent facial palsy in MRS, and prednisolone treatment was given to 6 cases who declined surgery. They were incorporated into surgery group and control group, respectively. Patients in surgery group and control group were followed up for 5.4 ± 1.4 years (range, 4 to 8 years) and 6.0 ± 1.4 years (range, 4 to 8 years), respectively. Results Further episodes of facial palsy affected none of 9 cases (0.0%) in surgery group, while they affected 3 of 6 cases (50.0%) in control group, with significant difference (p < 0.05). Conclusions Total facial nerve decompression was effective to prevent further episodes of facial palsy in MRS. © 2015 Published by Elsevier Inc. Source


Li W.L.,Xinxiang Medical University | Wang L.L.,Third Affiliated Hospital of Xinxiang Medical College | Luo Q.Y.,Third Affiliated Hospital of Xinxiang Medical College
The Scientific World Journal | Year: 2013

To develop a new facile protocol for the synthesis of 2′- aminobenzothiazolo-arylmethyl-2-naphthol derivatives, N-bromosuccinimide (NBS) was used as an efficient catalyst for the one-pot synthesis of 2′-aminobenzothiazolo-arylmethyl-2-naphthols in excellent yields from β-naphthol (1 mmol), aromatic aldehydes (1 mmol), and 2-aminobenzothiazole (1 mmol) at 60°C under solvent-free conditions. © 2013 Wei Lin Li et al. Source


Zhang K.,Third Affiliated Hospital of Xinxiang Medical College | Guo Q.,Third Affiliated Hospital of Xinxiang Medical College | Wang W.,Third Affiliated Hospital of Xinxiang Medical College | Li J.,Yellow River Technical College Affiliated Hospital of Zhengzhou | Li Q.,Yellow River Technical College Affiliated Hospital of Zhengzhou
Life Science Journal | Year: 2013

Objective To investigate the occurrence of biological structure change of the eye and its risk factors diabetic retinopathy. Methods In our hospital in January 2009 to December 2012, 100 cases were treated of diabetic patients with retinopathy study group, and at the same period 100 cases of diabetic retinopathy in patients were selected with non-control group. The two groups of patients with clinical data were analyzed retrospectively. Results Both corneal diameter and corneal curvature was not statistically significant (P>0.05); axial length and anterior chamber depth comparison with a significant difference (P<0.05). Diabetic retinopathy through single factor Logistic regression analysis, axial length, anterior chamber depth, duration of time are risk factors for diabetic retinopathy, (OR = 12.241,8.213,9.631, P<0.05). After other factors Logistic regression analysis was not statistically significant (P> 0.05). Conclusions Patients with diabetic retinopathy single course of time the main factors and Creactive protein, glycated hemoglobin, and axial length and anterior chamber depth, and axial length, anterior chamber depth, duration of time are risk factors for diabetic retinopathy. Diabetic retinopathy has a variety of factors, clinical should be taken seriously enough, as soon as the intervention of the relevant factors, thereby reducing diabetic retinopathy happen. Source

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