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Chen S.,Third Affiliated Hospital of Liaoning Medical College
Journal of Research in Medical Sciences | Year: 2014

Granulomatous mastitis (GM) is a rare breast disease with unknown etiology. Clinical management strategies for GM include surgery, antibiotics, and steroid treatments. As patients with GM often respond to steroids, GM is thought to be an autoimmune disease. Here we describe a case of trauma‑induced GM that presented as autoimmune disease but was successfully treated by surgery without steroids. The patient showed no sign of recurrence for 11 months. This case provides useful information on both the underlying mechanisms and clinical management of GM. © 2014, Isfahan University of Medical Sciences(IUMS). All rights reserved. Source


Liu J.-S.,First Affiliated Hospital of Liaoning Medical College | Wang Y.-D.,Third Affiliated Hospital of Liaoning Medical College | Li Y.-X.,First Affiliated Hospital of Liaoning Medical College | Ha M.-W.,First Affiliated Hospital of Liaoning Medical College | Liu Z.-L.,First Affiliated Hospital of Liaoning Medical College
Tumor | Year: 2012

Objective: To observe the induced effect of low dose of tunicamycin on endoplasmic reticulum stress (ERS) of A549 human lung adenocarcinoma cells in vitro and the change of Bcl-2 associated athanogene 1 (BAG-1) protein expression, as well as the changes of cisplatin (DDP) sensitivity and the BAG-1 protein expression. Methods: The expressions of GRP78 (a marker protein in ERS) and BAG-1 proteins in A549 cells treated with low dose of tunicamycin were detected by Western blotting. The change of half inhibitory concentration (IC 50) of DDP for A549 cells after pretreatment with tunicamycin was measured by MTT method, and the DDP sensitivity was determined by flow cytometry (FCM). The Western blotting was carried out to detect the expressions of BAG-1 and procaspase-12 proteins in A549 cells induced by DDP after pretreatment with tunicamycin. Results: The ERS in A549 cells could be induced by pretreatment with 1.25 μg/mL tunicamycin for 8 h, which displayed that the expression levels of GRP78 and BAG-1 proteins were both up-regulated (P <0.05). The ERS could increase the DDP sensitivity in the A549 cells (P <0.05). Before ERS occurred, all of three doses of DDP (1.25, 2.5 and 5 μg/mL) could up-regulate the expression level of BAG-1 protein (P <0.05), and this effect was more obvious in 1.25 μg/mL DDP-treated A549 cells. When ERS occurred, the expression levels of BAG-1 protein in all of three doses of DDP (1.25, 2.5 and 5 μg/mL)-treated A549 cells were down-regulated in a dosedependent manner as compared with that in the A549 cells without DDP treatment (P <0.05). The DDP dose of 2.5 and 5 μg/mL could induce apoptosis by ERS pathway, and the expression levels of BAG-1 and procaspase-12 proteins were both down-regulated during this process (P <0.05). Conclusion: BAG-1 may be one of the important regulatory factors in both pathways of ERS- and cisplatin-induced apoptosis of lung cancer cells, and ERS-related apoptotic pathway may be one of the important pathways of cisplatininduced apoptosis of lung cancer cells. Copyright © 2012 by TUMOR. Source


Zhang X.-L.,First Affiliated Hospital of Liaoning Medical College | Zheng S.-L.,First Affiliated Hospital of Liaoning Medical College | Dong F.-R.,First Affiliated Hospital of Liaoning Medical College | Wang Z.-M.,Third Affiliated Hospital of Liaoning Medical College
Journal of International Medical Research | Year: 2012

OBJECTIVE: To study the effect of nimodipine on hippocampal regional cerebral blood flow (rCBF) and proinflammatory cytokines in rats with experimental vascular dementia. METHODS: Male Sprague Dawley rats were randomly divided into four groups (n = 15/group): sham operated controls (group A); focal cerebral ischaemia (group B); vascular dementia (group C); and vascular dementia treated with 20 mg/kg nimodipine daily (group D). The Morris water maze test evaluated learning and memory, and magnetic resonance perfusion-weighted imaging was used to measure rCBF. Hippocampal levels of nuclear factor-κB (NF-κB), tumour necrosis factor-α (TNF-α) and interleukin 1b (IL-1β) were measured. RESULTS: Compared with group C, rats in group D demonstrated significantly improved learning ability and significantly increased hippocampal rCBF. The levels of NF-kB, TNF-α and IL-1β were significantly lower in group D than in group C. Hippocampal nerve cell morphology was abnormal in group C but near normal in group D. CONCLUSIONS: Nimodipine improved the symptoms of cognitive impairment, increased rCBF, reduced hippocampal cytokine levels and alleviated neuronal injury in the hippocampus of rats with experimental vascular dementia. © 2012 Field House Publishing LLP. Source


Wang G.,Liaoning Medical University | Wang G.,Third Affiliated Hospital of Liaoning Medical College | Ren G.,Liaoning Medical University | Cui X.,Liaoning Medical University | And 7 more authors.
Molecular Medicine Reports | Year: 2016

The interaction between the host and human cytomegalovirus (HCMV) is important in determining the outcome of a viral infection. The HCMV RL13 gene product exerts independent, inhibitory effects on viral growth in fibroblasts and epithelial cells. At present, there are few reports on the interactions between the HCMV RL13 protein and human host proteins. The present study provided direct evidence for the specific interaction between HCMV RL13 and host nucleoside diphosphate linked moiety X (nudix)-type motif 14 (NUDT14), a UDP-glucose pyrophosphatase, using two-hybrid screening, an in vitro glutathione S-transferase pull-down assay, and co-immunoprecipitation in human embryonic kidney HEK293 cells. Additionally, the RL13 protein was shown to co-localize with the NUDT14 protein in the HEK293 cell membrane and cytoplasm, demonstrated using fluorescence confocal microscopy. Decreasing the expression level of NUDT14 via NUDT14-specific small interfering RNAs increased the number of viral DNA copies in the HCMV-infected cells. However, the overexpression of NUDT14 in a stably expressing cell line did not affect viral DNA levels significantly in the HCMV infected cells. Based on the known functions of NUDT14, the results of the present study suggested that the interaction between the RL13 protein and NUDT14 protein may be involved in HCMV DNA replication, and that NUDT14 may offer potential in the modulation of viral infection. Source


Zhang Y.,Third Affiliated Hospital of Liaoning Medical College | Liu L.,First Affiliated Hospital of Liaoning Medical College | Li C.,First Affiliated Hospital of Liaoning Medical College | Ai H.,Third Affiliated Hospital of Liaoning Medical College
Cancer Biomarkers | Year: 2014

BACKGROUND: The expressions of leptin and its receptor (ObR) have been observed in human endometrial cancer (EC) cells, and leptin can promote the proliferation of EC cells. However, the correlation between leptin and ObR expressions in EC and the clinicopathology of EC is still unclear. OBJECTIVE: This study investigated the correlation between the expressions of leptin and ObR in EC and the clinicopathology of EC. METHODS: Immunohistochemistry was used in this study. The correlation between the expressions of leptin and ObR in EC and the clinicopathology of EC was analyzed. RESULTS: In the EC specimens, the expressions of leptin and ObR were positively correlated with the invasiveness of the cancer and the obesity of patients, but inversely correlated with histological grade. The percentages of leptin and ObR were significantly higher in the patients with lymph node metastasis. Positive expression of leptin and ObR was associated with poorer prognosis (3-year survival rate). Moreover, the expression of leptin and ObR was associated with positive expression of estrogen receptor. CONCLUSIONS: Leptin and ObR are overexpressed in EC, and their expressions are associated with malignancy, invasion, and metastasis of EC. Thus, leptin and ObR may be important indicators in EC. © 2014 - IOS Press and the authors. All rights reserved. Source

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