Feng H.-X.,Third Affiliated Hospital of Inner Mongolian Medical College |
Xin Y.,Third Affiliated Hospital of Inner Mongolian Medical College |
Hao Y.-Q.,Third Affiliated Hospital of Inner Mongolian Medical College |
Kang C.-Y.,Third Affiliated Hospital of Inner Mongolian Medical College
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: In recent years, pentoxifylline has been found to have a wide range of anti-fibrosis capacity. However, there are few studies explore the suppress effect of pentoxifylline on fibroblasts in human keloid, and the maximum inhibitory concentration remains poorly understood. OBJECTIVE: To study the effect of pentoxifylline on proliferation activity of human keloid fibroblasts and to select the maximum inhibitory concentration. METHODS: Human keloid fibroblasts were used as original cells, passaged till the 5 thto the 8thgenerations, and then divided into the experimental and control groups. Pentoxifylline with concentrations of 0.1, 0.25, 0.5, 1.0, 2.0 and 3.0 g/L were added to the experimental group. The effects of different concentrations of pentoxifylline on proliferation of keloid fibroblasts were detected by MTT chromometry. RESULT AND CONCLUSION: Compared with the control group, the inhibitory effect of pentoxifylline on the proliferation of keloid fibroblasts was more evident in the experimental group (P < 0.05). The inhibition rates of pentoxifylline on proliferation of keloid fibroblasts showed apparently time-and dose-effect relationships within the concentration of 0.1-2.0 g/L, which presented a greatest level at 96 hours after culture. The maximum inhibitory rate was 53.37%, and the concentration was 2.0 g/L in the experimental group. Consequently, pentoxifylline plays a notable inhibitory role in the proliferation of human keloid fibroblasts with concentration of 2.0 g/L at 96 hours after culture.