Thermo Fisher Scientific

Waltham, MA, United States

Thermo Fisher Scientific

Waltham, MA, United States

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Patent
Thermo Fisher Scientific | Date: 2016-09-29

A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, I553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is I553V, this is not in combination with D551S.


Patent
Thermo Fisher Scientific | Date: 2017-03-08

An operating value of a first laser parameter of a laser device in a laser absorption spectrometer is optimised. The wavelength of laser device emitted light is adjusted by the first or a second laser parameter. The laser absorption spectrometer comprises a light intensity detector measuring the laser light intensity from the laser device. For each of multiple values of the first laser parameter: the light intensity detector measures light intensity obtained across a range of second laser parameter values, and an extremum in the light intensity measure and a peak position for the extremum are identified. A range of first laser parameter values is identified within the values of the first laser parameter for which there is a continuous trend in changes to the identified peak position with changes to the first laser parameter. The first laser parameter operating value is set to be within the identified range.The laser device is either a mid-infrared LD or the difference frequency generated in a PPNL (250) using the output of two near infrared telecommunication laser diodes (210,220) having for example a DFB (220). The wavelength sweep is realized by a corresponding control of temperature and bias current provided to the LD while the absorption peaks of the isotopes to be detected should be in the middle of the individual sweep ranges.


Patent
Thermo Fisher Scientific | Date: 2017-01-18

Monomethine dyes that have no or minimal fluorescence in buffer or in the presence of single stranded DNA or RNA, but strongly fluorescence in the presence of double-stranded DNA. In one embodiment, the dye is useful in quantitative RT-PCR.


Patent
Thermo Fisher Scientific | Date: 2017-01-18

A composition for the removal of protein contaminants from a solution containing target nucleic acid, which composition comprises: (i) a polyacid, which comprises a polycarboxylic acid, a polyphosphonic acid or a polycarboxylate or polyphosphonate salt; and (ii) a solid phase having a surface which comprises a functionalised silica, wherein the solid phase comprises silica, wherein the solid phase optionally comprises particles, wherein the particles are optionally approximately spherical and have a diameter in the range of from 3 to 15 m, and wherein the particles optionally have pores with a diameter of from 10 to 100 nm.


Patent
Thermo Fisher Scientific | Date: 2017-01-04

An electrostatic trap such as an orbitrap is disclosed, with an electrode structure. An electrostatic trapping field of the form U(r, , z) is generated to trap ions within the trap so that they undergo isochronous oscillations. The trapping field U(r, , z) is the result of a perturbation W to an ideal field U(r, , z) which, for example, is hyperlogarithmic in the case of an orbitrap. The perturbation W may be introduced in various ways, such as by distorting the geometry of the trap so that it no longer follows an equipotential of the ideal field U(r, , z), or by adding a distortion field (either electric or magnetic). The magnitude of the perturbation is such that at least some of the trapped ions have an absolute phase spread of more than zero but less than 2 radians over an ion detection period T_(m).


Patent
Thermo Fisher Scientific | Date: 2017-04-19

A pipette (1, 21) comprising a cylinder (10) with a piston (11) movable inside the cylinder for aspiring and dispensing liquid, and a handle portion (2) for gripping the pipette, wherein the pipette (1, 21) further comprises an imaging device element (16) for obtaining images that assist the use of the pipette.


The invention relates to a cap (16) for closing a reagent bottle and a gripper for handling an inner part of the closing cap (2). According to the invention, the inner part of the closing cap (2) has a recess (6) for gripping the cap (2) by applying a vacuum. The invention relates also to a system for handling closures, a method for removing a closing cap from a reagent bottle, and a related gripper.


Patent
Thermo Fisher Scientific | Date: 2017-04-19

A data processing device according to the invention comprises a processor unit adapted to process a plurality of initial data vectors provided by a chromatograph and/or a mass spectrometer, the processing being carried out in one, two or more processing steps producing items of processed data, and a storage unit adapted to save and retrieve initial data vectors and/or items of processed data, in particular processed data vectors or identified compounds, and/or items of additional data, in particular properties of the sample introduced in the mass spectrometer. Each item of processed data and/or additional data is connected to at least one initial data vector, and wherein the processor unit is adapted to group, select and/or modify initial data vectors and/or items of processed data according to one or more items of additional data. The invention further pertains to a mass spectrometry setup and a method for identifying and/or quantifying peptides and/or proteins and/or metabolites.


Patent
Thermo Fisher Scientific | Date: 2017-01-13

This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3-5 exonuclease domain and/or a sequence non-specific dsDNA binding domain.


Patent
Thermo Fisher Scientific | Date: 2017-01-13

This disclosure relates to antibodies that bind thermophilic family B DNA polymerases. Related uses, methods, and compositions are also provided.

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