Waltham, MA, United States
Waltham, MA, United States

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Patent
Thermo Fisher Scientific | Date: 2016-09-29

A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, I553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is I553V, this is not in combination with D551S.


Patent
Thermo Fisher Scientific | Date: 2016-11-29

A sample preparation and analysis system. The system 10 includes a sample preparation system 12 and a sample analysis system 14. The sample preparation system 12 prepares samples in accordance with an assay that is selected from a database containing a plurality of unique assays. The sample analysis system 14 includes an analyzer 110 that is dynamically reconfigurable based on the selected assay so as to analyze the prepared sample in accordance with that selected assay. A data communication link 27 communicates data from the sample preparation system 12 to the sample analysis system 14 to reconfigure the analyzer 110 in accordance with the selected assay.


Patent
Thermo Fisher Scientific | Date: 2016-08-30

An operating value of a first laser parameter of a laser device in a laser absorption spectrometer is optimized. The wavelength of laser device emitted light is adjusted by the first or a second laser parameter. The laser absorption spectrometer comprises a light intensity detector measuring the laser light intensity from the laser device. For each of multiple values of the first laser parameter: the light intensity detector measures light intensity obtained across a range of second laser parameter values, and an extremum in the light intensity measure and a peak position for the extremum are identified. A range of first laser parameter values is identified within the values of the first laser parameter for which there is a continuous trend in changes to the identified peak position with changes to the first laser parameter. The first laser parameter operating value is set to be within the identified range.


Patent
Thermo Fisher Scientific | Date: 2016-07-01

A method for producing from host cells a heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state. The method cultured the host cells in a culture medium comprising a reducing agent; and recovered the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from the host cells or from the culture medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells.


A method for generating a mass spectrum of sample ions using a multi-collector mass spectrometer is disclosed. The mass spectrometer includes a spatially dispersive mass analyser to direct the sample ions into a detector chamber. The method includes generating sample ions of a first ion species A, a second ion species B, and a third ion species C, wherein the ions of species A have a different nominal mass to the ions of species B and C, and further wherein the ions of species B have the same nominal mass as the ions of species C. The sample ions of the species A, B and C are directed to travel through the mass analyser and towards detectors in the detector chamber, the sample ions being deflected during their travel. The ions of species B and C are scanned across a master aperture defined in a master mask of a master detector, while the ions of species A pass through a lead aperture defined in a lead mask of a lead detector. A lead signal is generated representing the ion intensity received at the lead detector from the ions of species A, and generating a master signal representing the ion intensity received at the master detector whilst the ions of species B and C are scanned across the master aperture. During scanning, ions of the species A are detected by the lead detector while ions of the species B but not C, then both species B and species C, and then species C but not B are detected by the master detector.


Patent
Thermo Fisher Scientific | Date: 2016-09-09

An elemental mass spectrometer uses a mass filter to select ions from ions received from an ion source and transmit the selected ions. A reaction or collision cell receives the transmitted ions and reacts or collides these with a gas to provide product ions thereby. A mass analyzer receives the product ions, analyzes them and provides at least one output based on detection of the analyzed ions. The elemental mass spectrometer is operated to provide a first output from the mass analyzer measuring ions within a first analysis range of mass-to-charge ratios including a desired mass-to-charge ratio, M, to provide a second output from the mass analyzer measuring ions within a second analysis range of mass-to-charge ratios including a mass-to-charge ratio at least 0.95 atomic mass units lower than the desired mass-to-charge ratio, (Mi), i0.95 and to correct the first output on the basis of the second output.


Patent
Thermo Fisher Scientific | Date: 2016-07-22

A method of imaging analyte elements in an organic sample includes providing the sample as a layer on a substrate and reacting the sample on the substrate to produce one or more volatile products that leave the sample while the one or more elements remain in the sample. A majority of the sample layer by weight is removed from the substrate by the reaction and the remaining sample layer is enriched in the one or more elements which are not spatially disturbed by the reaction. The method including subsequently detecting the one or more elements in the concentrated sample layer using an imaging elemental analyzer.


Patent
Thermo Fisher Scientific | Date: 2017-03-08

An operating value of a first laser parameter of a laser device in a laser absorption spectrometer is optimised. The wavelength of laser device emitted light is adjusted by the first or a second laser parameter. The laser absorption spectrometer comprises a light intensity detector measuring the laser light intensity from the laser device. For each of multiple values of the first laser parameter: the light intensity detector measures light intensity obtained across a range of second laser parameter values, and an extremum in the light intensity measure and a peak position for the extremum are identified. A range of first laser parameter values is identified within the values of the first laser parameter for which there is a continuous trend in changes to the identified peak position with changes to the first laser parameter. The first laser parameter operating value is set to be within the identified range.The laser device is either a mid-infrared LD or the difference frequency generated in a PPNL (250) using the output of two near infrared telecommunication laser diodes (210,220) having for example a DFB (220). The wavelength sweep is realized by a corresponding control of temperature and bias current provided to the LD while the absorption peaks of the isotopes to be detected should be in the middle of the individual sweep ranges.


Patent
Thermo Fisher Scientific | Date: 2017-01-18

Monomethine dyes that have no or minimal fluorescence in buffer or in the presence of single stranded DNA or RNA, but strongly fluorescence in the presence of double-stranded DNA. In one embodiment, the dye is useful in quantitative RT-PCR.


Patent
Thermo Fisher Scientific | Date: 2017-01-18

A composition for the removal of protein contaminants from a solution containing target nucleic acid, which composition comprises: (i) a polyacid, which comprises a polycarboxylic acid, a polyphosphonic acid or a polycarboxylate or polyphosphonate salt; and (ii) a solid phase having a surface which comprises a functionalised silica, wherein the solid phase comprises silica, wherein the solid phase optionally comprises particles, wherein the particles are optionally approximately spherical and have a diameter in the range of from 3 to 15 m, and wherein the particles optionally have pores with a diameter of from 10 to 100 nm.

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