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Chaisi M.E.,University of Pretoria | Chaisi M.E.,National University of Lesotho | Janssens M.E.,Institute of Tropical Medicine | Janssens M.E.,Therapeutic Systems | And 4 more authors.
PLoS ONE | Year: 2013

A quantitative real-time PCR (qPCR) assay based on the cox III gene was evaluated for the simultaneous detection and discrimination of Theileria species in buffalo and cattle blood samples from South Africa and Mozambique using melting curve analysis. The results obtained were compared to those of the reverse line blot (RLB) hybridization assay for the simultaneous detection and differentiation of Theileria spp. in mixed infections, and to the 18S rRNA qPCR assay results for the specific detection of Theileria parva.Theileria parva, Theileria sp. (buffalo), Theileria taurotragi, Theileria buffeli and Theileria mutans were detected by the cox III assay. Theileria velifera was not detected from any of the samples analysed. Seventeen percent of the samples had non-species specific melting peaks and 4.5% of the samples were negative or below the detection limit of the assay. The cox III assay identified more T. parva and Theileria sp. (buffalo) positive samples than the RLB assay, and also detected more T. parva infections than the 18S assay. However, only a small number of samples were positive for the benign Theileria spp. To our knowledge T. taurotragi has never been identified from the African buffalo, its identification in some samples by the qPCR assay was unexpected.Because of these discrepancies in the results, cox III qPCR products were cloned and sequenced. Sequence analysis indicated extensive inter- and intra-species variations in the probe target regions of the cox III gene sequences of the benign Theileria spp. and therefore explains their low detection. The cox III assay is specific for the detection of T. parva infections in cattle and buffalo. Sequence data generated from this study can be used for the development of a more inclusive assay for detection and differentiation of all variants of the mildly pathogenic and benign Theileria spp. of buffalo and cattle. © 2013 Chaisi et al. Source

Therapeutic Systems | Date: 2013-02-06

Light-based medical devices, namely, a light emitting diode (LED) device for increasing the human bodys own production of collagen.

News Article | October 24, 2011
Site: www.xconomy.com

It’s getting tiring to hear that the United States can’t. Can’t innovate anymore. Can’t create jobs. But if you look around Massachusetts I think you’ll come to the conclusion that if anyone can, it’s probably people under 30 (plus or minus). I’m sitting in an old mill building in Holyoke. There are almost 100,000 students centered up and down the Connecticut River Valley. There’s a conference going on called Idea Mill on entrepreneurship and innovation. Brendan Ciecko, Ben Einstein, and his friends who created this event believe they can catalyze a reinvention of this place. They’re under 30. They probably can. On October 24 MassChallenge, the globally encompassing, Massachusetts-centric $1 million startup competition and accelerator will have its final event. If you look around the room that night you’ll see hundreds of young entrepreneurs who believe they can do a startup in Massachusetts and create jobs and innovation. My organization, the National Collegiate Inventors and Innovators Alliance, is thrilled that three of the MassChallenge finalists are ventures started with our grants. Therapeutic Systems is working to shift the daily experience of people with autism with its sophisticated but discrete pressure vest that provides a “hug-like” sensation to the wearer. Brian Mullen believes he can not only make a cash positive business but get through the medical reimbursement process as a young entrepreneur and manufacture the product in Massachusetts. He’s under 30. He probably can. SmarterShade is developing a product to electronically darken a window with the flip of a switch. Will McLeod has never moved an innovative product from prototype to manufacturing to product. But he’s under 30. He probably can. Finally, Sanergy thinks they can transform the sanitation system in extremely poor neighborhoods in Kenya and throughout the world. They’ve got one of their pay toilets in operation. In a week or so they’ll have 10 times that. By early next year hopefully 10 times that. Can David Auerbach and his young team create a profitable global business based on people paying a few cents per use? They probably can. Those of us who can no longer even fake that we’re young have increasingly despaired for the trajectory of this country in recent years. Who’s going to create the jobs and the Next Big Thing? Show up at an event like Idea Mill or MassChallenge and you’ll quickly run into the people who can. Joseph Steig directs VentureWell for NCIIA and is also CFO of Long River Ventures. Follow @

Tamura A.,National Defense Medical College | Matsunobu T.,National Defense Medical College | Tamura R.,National Defense Medical College | Kawauchi S.,Therapeutic Systems | And 2 more authors.
Brain Research | Year: 2016

Photobiomodulation (PBM) is a noninvasive treatment that can be neuroprotective, although the underlying mechanisms remain unclear. In the present study, we assessed the mechanism of PBM as a novel treatment for noise-induced hearing loss, focusing on the nuclear factor (NF)-κB signaling pathway. Sprague–Dawley rats were exposed to 1-octave band noise centered at 4 kHz for 5 h (121 dB). After noise exposure, their right ears were irradiated with an 808 nm diode laser beam at an output power density of 165 mW/cm2 for 30 min a day for 5 consecutive days. Measurement of the auditory brainstem response revealed an accelerated recovery of auditory function in the groups treated with PBM compared with the non-treatment group at 4, 7, and 14 days after noise exposure. Immunofluorescent image analysis for inducible nitric oxide synthase and cleaved caspase-3 showed lesser immunoreactivities in outer hair cells in the PBM group compared with the non-treatment group. However, immunofluorescent image analysis for NF-κB, an upstream protein of inducible nitric oxide synthase, revealed greater activation in the PBM group compared with the naïve and non-treatment groups. Western blot analysis for NF-κB also showed stronger activation in the cochlear tissues in the PBM group compared with the naïve and non-treatment groups (p<0.01, each). These data suggest that PBM activates NF-κB to induce protection against inducible nitric oxide synthase-triggered oxidative stress and caspase-3-mediated apoptosis that occur following noise-induced hearing loss. © 2016 Elsevier B.V. Source

Reynolds S.,Virginia Commonwealth University | Lane S.J.,Virginia Commonwealth University | Mullen B.,Therapeutic Systems
The American journal of occupational therapy : official publication of the American Occupational Therapy Association | Year: 2015

Deep pressure stimulation has been used in therapeutic practice because of the assumption that it changes physiological arousal. The purpose of this study was to test the effects of deep pressure stimulation, applied with a Vayu Vest (Therapeutic Systems), on both autonomic arousal and performance in a normative adult sample. A repeated-measures, repeated-baseline design was used with participants completing a performance test before and after deep pressure application. A convenience sample of 50 adults participated in the study. Results showed that wearing the Vayu Vest for even short periods of time reduced sympathetic arousal and non-stimulus-driven electrical occurrences. Concomitant increases in parasympathetic arousal were found. Performance improvements were noted after wearing the Vayu Vest, potentially because of changes in arousal. We conclude that deep pressure stimulation is capable of eliciting changes in autonomic arousal and may be a useful modality in diagnostic groups seen by occupational therapy practitioners. Copyright © 2015 by the American Occupational Therapy Association, Inc. Source

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