Yang Q.,Uniformed Services University of the Health Sciences |
Dalgard C.,Uniformed Services University of the Health Sciences |
Eidelman O.,Uniformed Services University of the Health Sciences |
Jozwik C.,Uniformed Services University of the Health Sciences |
And 3 more authors.
Journal of Carcinogenesis | Year: 2013
Background : Cardiac glycosides such as digitoxin have been shown to directly cause apoptotic death of cancer cells both in vitro, and in vivo. However, the mechanism connecting cardiac glycoside action to apoptosis is not known. It has been reported that compounds resembling digitoxin are able to reduce c-MYC expression. Furthermore, it has been previously shown that the transcription of c-MYC depends on nuclear factor of activated T-cells (NFAT) binding sites in the c-MYC promoter. We have therefore hypothesized that NFAT might mediate digitoxin effects on c-MYC mRNA message. Materials and Methods : We have chosen to study this process in HeLa cells where structurally intact c-MYC genes in 8q24 co-localize with human papilloma virus 18 at all integration sites. Results : Here we show that within the 1 st h following treatment with digitoxin, a significant reduction in c-MYC mRNA occurs. This is followed by a precipitous loss of c-MYC protein, activation of caspase 3, and subsequent apoptotic cell death. To test the NFAT-dependence mechanism, we analyzed the effects of digitoxin on NFAT isoform-dependent auto-activation of a NFAT-luciferase expression system. Drug dependent effects on expression varied according to each of the four canonical NFAT isoforms (1, 2, 3 or 4). The most digitoxin-sensitive NFAT isoform was NFAT1. Using c-MYC chromatin immune precipitation, we find that digitoxin inhibits interaction of NFAT1 with the proximal c-MYC promoter. Conclusions : These results suggest that the carcinotoxic activity of digitoxin includes suppression of NFAT-driven c-MYC expression. © 2012 Warren.
Landrum C.,Amec Foster Wheeler |
Castrignano A.,Crea - Tec |
Zourarakis D.,The United States Information Technology Office |
Mueller T.,Deere & Company
Agricultural Water Management | Year: 2016
Understanding the variation of soil moisture patterns over time can support field investigative efforts specific to managing irrigation input, environmental risk assessment, or water resource planning. The objective of this study was to apply an integrated suite of traditional (e.g. Vachaud's time stability analysis) and less common techniques (e.g. confusion matrix, Castrignanò’s average of the differences, cross correlogram analysis, and polygon kriging) to gain deeper insight into the temporal persistence of soil moisture patterns, especially for site-specific water management purposes. This study used soil moisture estimates generated in previous work that was carried out in a field in Central Kentucky for three dates ranging from permanent wilting point up to field capacity. The results obtained from this study provide richer evidence of time stable soil moisture patterns and lend greater insight into the controlling factors of spatial and temporal variation, including soil moisture status, soil physicochemical properties, and landscape position, that otherwise would not be attainable using a single metric alone. © 2016 Elsevier B.V.
Mateik D.,The United States Information Technology Office
Proceedings ACM SIGUCCS User Services Conference | Year: 2010
Exposing faculty to one another's instructional challenges and accomplishments and enabling them to network has been the motivation behind an annual conference at the University of Maryland for nearly 20 years. The instructional technology experiences of three intrepid early adopters were showcased in the University's first half-day Teaching with Technology symposium in 1993. By 1999 the symposium blossomed into a full-day conference where presentations by 12 instructors continued to emphasize different forms of technology in the classroom, but, oddly enough, integrated only minimal use of technology in the presentations themselves. As technology in the classroom became ubiquitous, the sponsoring conference hosts believed it was time to change the focus of the conference from technology in teaching to innovation in teaching and learning. This paper will briefly trace lessons learned in the evolution of the Innovations in Teaching and Learning Conference . It will additionally focus on our recent integration of technologies such as Twitter, lecture capture tools, QR codes and streamed broadcasting, which enable us to model uses of instructional technology tools and to make the conference available to a broader audience. © 2010 ACM.
Osnes B.,University of Colorado at Boulder |
Gammon M.,The United States Information Technology Office
Sustainability | Year: 2013
Are brief, single-issue videos an effective way to illuminate issues of sustainability online? Can web-based performance ignite positive social change? This article tracks an attempt by Beth Osnes and Mark Gammon to answer those questions. This article is primarily a methodological reflection of our pilot effort and recommendations for future attempts to accurately assess the effectiveness of web-based performance for social change.
Donald C.,The United States Information Technology Office
Proceedings ACM SIGUCCS User Services Conference | Year: 2011
In this paper, I describe the process the Office of Information Technology (OIT) followed in creating our Computing @ newsletter and other print materials while meeting our university branding requirements. I also describe our methodology for teaching these same processes to members of our faculty, staff, and graduate departments. It is not my intention to provide a word for word example of my presentation. Since the materials I will be referencing are all available online, it seems unnecessary to reproduce them in their entirety here. I will go into each section in greater detail as I speak about our specific experiences in regards to these topics.
Zourarakis D.P.,The United States Information Technology Office
American Society for Photogrammetry and Remote Sensing Annual Conference 2010: Opportunities for Emerging Geospatial Technologies | Year: 2010
In Kentucky's Eastern Coal Fields physiographic region, mining and reclamation activities often result in stream modification, potentially leading to the creation of new ponds and reservoirs. Incorporation of these changes as part of updates to the National Hydrography Dataset is proceeding slowly. The 2001-2005 Kentucky Landscape Census modernization of the NLCD01 demonstrated the extremely dynamic characteristic of the landscape in that region of the state where major land cover changes are due to resource extraction. Timely, in-situ monitoring and assessment of waterbodies created or modified in permitted mining operations would prove costly for government agencies. This paper explores the use of multitemporal change analysis, based on Landsat 5 TM and aerial, multispectral imagery for the detection of new waterbodies in areas affected by mining activities.
Kelly W.R.,U.S. National Institute of Standards and Technology |
Pratt K.W.,U.S. National Institute of Standards and Technology |
Guthrie W.F.,U.S. National Institute of Standards and Technology |
Martin K.R.,The United States Information Technology Office
Analytical and Bioanalytical Chemistry | Year: 2011
Origin and early history of Die Methode des Eichzusatzes or The Method of Standard Addition with primary emphasis on its origin, early design, dissemination, and usage of terms is discussed. Die Methode des Eichzusatzes or literally 'the method of calibration addition' was first used and described by Hans Hohn in 1937 in his book Chemische Analysen mit dem Polarographen. Polarography was a very popular and much used technique well into the 1960s until the advent of modern spectroscopy. The literal English translation of Eichzusatzes into calibration addition comes from joining the verb, eichen, and the noun, Zusatz, which mean to calibrate and addition, respectively. The two-level design described by Kolthoff and Lingane was the design originated by Hohn and subsequently used by all early polarographers.
Palumbo J.,The United States Information Technology Office |
Becchi M.,The United States Information Technology Office |
Way S.,American University of Washington
SIGUCCS'12 - ACM Proceedings of the SIGUCCS Annual Conference | Year: 2012
American University's Office of Information Technology (OIT) training unit provides classroom-based and online training to over 1,800 staff, students, and faculty attendees annually. This group develops, schedules, markets, and delivers the training. The training unit also evaluates the effectiveness of the training and provides detailed reporting for the university's staff performance management program. In 2011, the OIT training unit was invited to partner with the University's Human Resources department and other campus trainers to select a vendor and implement an online Learning Management System for full-time university staff, and faculty with supervisory responsibilities. The training partners went through a lengthy assessment process, chose a vendor, assigned system roles, attended extensive administrator training, and then the hard work began! Although some of the training partners shared similar technological tools and processes, generally each group had a distinct, and well-established, method for managing the administration, marketing, and assessment of their training program. Combining these methods, while maintaining each group's autonomy proved challenging. This presentation will discuss how the team leveraged the learning management technology to meet our shared goals, culminating in a successful system launch in February, 2012. We will explore how we selected the technical components we would utilize, and how we developed a common language and unified processes. We will also discuss the process of branding our new system as "ULearn," how we created a ULearn portal, and how we marketed the system. Lastly, we will explore how we are fostering a "ULearn More" environment by empowering our customers to navigate their own learning path. Copyright © 2012 ACM.
Xin J.,The United States Information Technology Office
Resource: Engineering and Technology for Sustainable World | Year: 2012
The Subscription Management System (SMS) developed at University of Florida/Institute of Food and Agricultural Sciences (UF/IFAS) is a distributed subscription and Extension clientele management system. This centralized system contains information about Extension program areas, newsletters, and clientele profiles. Extension agents can communicate with their clientele with personalized and relevant information tailored to a group of clients. The system provides Extension faculty with reports on clientele activities and their profiles to better understand their interests and to improve the delivery of Extension programs. The Distance Diagnostic and Identification System (DDIS) allows growers, Master Gardeners, Extension agents, and specialists at UF/IFAS and other locations to collaborate on the diagnosis of infestations, diseases, and other agricultural problems. EDIS, an Extension publication system at UF/IFAS, provides a wide range of information at users' fingertips. 0.