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Wang Y.,Jilin University | Li H.,The Tumor Hospital of Jilin Province | Bao H.,Jilin University | Jin Y.,Jilin University | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

The aim of this study was to study the expression level of interferon-γ (IFN-γ) and monocyte chemoattractant protein-1 (MCP-1) in peripheral blood and its auxiliary diagnostic value in active tuberculosis. A chemiluminescence enzyme immunoassay method was used to detect the levels of IFN-γ and MCP-1 in peripheral blood. Then the receiver operating characteristic curve were drawn to determine the threshold of IFN-γ and MCP-1 for diagnosis of active tuberculosis and to evaluate their diagnostic performance. The specific IFN-γ and MCP-1 levels in the active tuberculosis group were significantly higher than those in the non-tuberculous pulmonary disease group (P < 0.01) and those in the healthy control group (P < 0.01). The IFN-γ levels in the healthy control group and the non-tuberculous respiratory disease group showed no statistically significant difference (P > 0.05), but the MCP-1 levels in the non-tuberculous respiratory disease group were significantly higher than those of the healthy control group (P < 0.05). The specific IFN-γ and MCP-1 level cut off values were 256 pg/ml and 389 pg/ml as an active tuberculosis diagnostic standard. The sensitivities of IFN-γ and MCP-1 were 57.3% and 92.8%, respectively; specificities were 80% and 80.7%, respectively; the positive predictive values were 76.9% and 84.9%, respectively; negative predictive values were 61.7% and 78.7%, respectively; and accuracy rates were 76.9% and 84.9%, respectively. Compared with the detection of IFN-γ, we observed a better diagnostic performance of MCP-1 in peripheral blood in active tuberculosis. MCP-1 may become one of the active tuberculosis auxiliary diagnostic targets. © 2015, E-Century Publishing Corporation. All rights reserved.

Cui Y.,Changchun University of Science and Technology | Zhao X.,The Tumor Hospital of Jilin Province | Wang L.,Changchun University of Science and Technology | Zhang J.,Changchun University of Science and Technology | Zhang W.,Changchun University of Science and Technology
Kuei Suan Jen Hsueh Pao/Journal of the Chinese Ceramic Society | Year: 2015

The microcapsule loaded with DNA was prepared with solid nanosilica microspheres as templates. Silane coupling agent was used as a glue to absorb DNA on the surface of SiO2. Amino groups on the surface can improve the electrostatic attraction between SiO2 and DNA. PEI and PAA were absorbed on the microspheres alternately due to the electrostatic attraction to form DNA loaded microspheres encapsulated with polymer layers. The process of layer was characterized by layer self-assembly via the zeta potential measurements. The multi-layer polymer enveloped microcapsules were obtained with DNA loaded after dissolving SiO2. According the analysis by transmission electron microscopy, particle size measurement, electrophoresis retardarce and gene transfection experiments, the microcapsules appear a hollow structure with spherical morphology and well-dispersion, which can block the DNA and realize gene transfection. ©, 2015, Chinese Ceramic Society. All right reserved.

He D.,Jilin University | He D.,Academy of Military Medical science of PLA | Sun L.,The Tumor Hospital of Jilin Province | Li C.,Academy of Military Medical science of PLA | And 10 more authors.
Viruses | Year: 2014

Oncolytic virotherapy has been an attractive drug platform for targeted therapy of cancer over the past few years. Viral vectors can be used to target and lyse cancer cells, but achieving good efficacy and specificity with this treatment approach is a major challenge. Here, we assessed the ability of a novel dual-specific anti-tumor oncolytic adenovirus, expressing the hemagglutinin-neuraminidase (HN) gene from the Newcastle disease virus under the human telomerase reverse transcriptase (hTERT) promoter (Ad-hTERTp-E1a-HN), to inhibit esophageal cancer EC-109 cells in culture and to reduce tumor burden in xenografted BALB/c nude mice. In vitro, infection with Ad-hTERT-E1a-HN could inhibit the growth of EC-109 cells significantly and also protect normal human liver cell line L02 from growth suppression in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Ad-hTERT-E1a-HN also effectively and selectively decreased the sialic acid level on EC-109 cells, but not on L02 cells. Furthermore, Ad-hTERT-E1a-HN was shown to induce the apoptosis pathway via acridine orange and ethidium bromide staining (AO/EB staining), increase reactive oxygen species (ROS), reduce mitochondrial membrane potential and release cytochrome c. In vivo, xenografted BALB/c nude mice were treated via intratumoral or intravenous injections of Ad-hTERT-E1a-HN. Although both treatments showed an obvious suppression in tumor volume, only Ad-hTERT-E1a-HN delivered via intratumoral injection elicited a complete response to treatment. These results reinforced previous findings and highlighted the potential therapeutic application of Ad-hTERT-E1a-HN for treatment of esophageal cancer in clinical trials. © 2014 by the authors; licensee MDPI, Basel, Switzerland.

Liu D.,The Tumor Hospital of Jilin Province | Yan L.,The Tumor Hospital of Jilin Province | Wang L.,The Tumor Hospital of Jilin Province | Tai W.,The Tumor Hospital of Jilin Province | And 2 more authors.
Oncology Letters | Year: 2014

Although cisplatin (DDP) has been reported to be a promising antitumor therapy for non‑small cell lung cancer (NSCLC), the effectiveness of the treatment remains limited due to an inherent tumor resistance to DDP. Genistein (GEN) is an abundant, naturally occurring isoflavonoid found in soy products that has been demonstrated to increase the anti‑neoplastic activity of certain chemotherapy drugs in multiple tumor types. In the present study, DDP in combination with GEN was selected as a potential treatment to suppress tumor growth and simultaneously reduce the doses of the two drugs required for the treatment of NSCLC. Cell growth inhibition, apoptosis, cell cycle distribution and receptor signaling assays were conducted. In the in vivo study, DDP and GEN, either alone or in combination, were used to treat a xenograft model of the A549 cells. It was found that the combination of low concentrations of DDP and GEN induced significantly greater growth inhibition (P< 0.01) and increased apoptosis in the A549 cells compared with either agent alone. In addition, DDP in combination with GEN could significantly suppress tumor growth in vivo compared with either agent alone. Combination treatment significantly suppresses constitutive phosphorylation of AKT and phosphoinositide‑3 kinase, which may contribute to the inhibition of tumor growth. Overall, the present data suggested that GEN can increase the anti‑neoplastic activity of DDP and that a combination of GEN and DDP is a potential drug candidate for the treatment of NSCLC. © 2014, Spandidos Publications. All rights reserved.

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