Li Y.-H.,Affiliated Hospital of Jining Medical College |
Li Y.-H.,The Peoples Hospital Of Jimo |
Li J.-E.,The Peoples Hospital Of Jimo |
Liu W.-W.,The Third Peoples Hospital Of Qingdao
International Journal of Clinical and Experimental Medicine | Year: 2017
Glaucoma is a chronic ocular disorder that occurs due to the degeneration of retinal ganglion cells (RGCs). Mesenchymal stem cells (MSCs) have emerged to be potential candidates to regenerate new cells and restore vision. There is an immediate need to find an efficient stem cell based therapy to enhance the secretion of neurotrophic factors (NFs) and promote long term cell survival. There are numerous mechanisms behind the regeneration of cells induced by the secretion of NFs that needs to be validated yet. DPSCs and BMSCs were isolated from the Sprague-Dawley rats and engineered into lentiviral constructs with BDNF and GDNF. The secretion of NFs by the engineered MSCs was quantified by ELISA and then transplanted into glaucoma rat model. The effect of NF secretion by the engineered MSCs on the neurite outgrowth was observed. The expression of myelin Nogo-A and OMgp, was investigated by RT-PCR and western blot analyses. The intraocular pressure in the eye of glaucoma rat model was reduced following the transplantation of the engineered stem cells. DPSCs were more efficient than BMSCs in secretion of neurotrophic factors (BDNF and GDNF) and enhanced the neurite outgrowth. Western blot analyses showed that Nogo-A and OMgp expression was significantly reduced. DPSCs are an alternate source of MSCs that are more efficient than BMSCs. The engineered MSCs secrete significantly higher amount of neurotrophic factors than the normal MSCs. The regeneration of RGCs following the optic nerve injury is associated with the reduced expression of Nogo-A and OMgp. © 2017, E-Century Publishing Corporation. All rights reserved.
PubMed | The Third Peoples Hospital of Qingdao
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2016
The aim of the present study was to examine the clinical merits of joint application of -sodium aescinate and mannitol for the treatment of early swelling of upper limb trauma after surgery. We verified whether the expression of serum aquaporin 1 (AQP-1) was involved in swelling mechanism. A total of 102 patients with swelling after upper limb trauma surgery were enrolled into the study and divided randomly into 3 groups (n=34 cases per group). Group A was treated with -sodium aescinate; group B was treated with with mannitol and group C was treated with both -sodium aescinate and mannitol. The expression level of AQP-1, and clinical effects and complications before and after treatment were compared. The time of swelling subsidence in group C was significantly shorter than that of the other two groups and differences were statistically significant (P<0.05). The recovery ratio and total efficiency in group C were significantly higher than those in other two groups and differences were statistically significant (P<0.05). Three and seven days after treatment, the AQP-1 levels in group A and group C were decreased and AQP-1 level decreased further with time. Differences of comparison within groups were statistically significant (P<0.05), although the differences of comparison between the groups showed no statistical significance (P>0.05). We also compared the AQP-1 level in group B before and after treatment, and the differences were not statistically significant (P>0.05). When the complication incidence in the 3 groups was compared, no statistical significance was detected (P>0.05). We concluded that the joint use of -sodium aescinate and mannitol in treating early swelling after upper limb trauma surgery produced satisfactory outcomes. This might be related to reduction of the AQP-1 level.
PubMed | The No 8 Peoples Hospital Of Qingdao, Qingdao University and The Third Peoples Hospital of Qingdao
Type: Journal Article | Journal: Molecular medicine reports | Year: 2015
Gastric cancer is one of the most frequent malignancies and a leading cause of cancer-related mortality worldwide. MicroRNAs (miRs), a class of small noncoding RNAs, have been shown to be critical in tumorigenesis. In the present study, the expression levels of miR132 were analyzed in gastric cancer samples using quantitative reverse transcriptionpolymerase chain reaction. In addition, the cell viability, proliferation and invasion abilities were determined in two gastric cancer cell lines, NCIN87 and MGC803, that were transfected with miR132 mimics or antisense oligos. It was found that miR132 expression was significantly upregulated in gastric cancer tissues when compared with adjacent noncancerous tissues. At the molecular level, the data demonstrated that miR132 inhibits the protein levels of retinoblastoma 1 (RB1) by targeting the 3untranslated region. Furthermore, reintroduction of RB1 markedly attenuated the proliferative roles of miR132 overexpression. Therefore, the present results indicate that the miR132/RB1 regulatory axis may be a potential novel diagnostic and therapeutic target for the treatment of gastric cancer.
PubMed | The Chen Group, Qingdao Hiser Medical Group and The Third Peoples Hospital of Qingdao
Type: Journal Article | Journal: Pakistan journal of medical sciences | Year: 2016
To detect the risk factors for deep venous thrombosis (DVT) in patients after neurosurgery.Three hundred and seventy-six patients treated in the department of neurosurgery of our hospital from February 2013 to November 2015 were reviewed retrospectively. The clinical data including age, gender, hospital stay, operation time, occupation type, hypertension, coronary heart disease, diabetes, smoking status, drinking status, postoperative exercises, malignant tumor, and postoperative hormone or dehydrating agent were collected.In this study, 52 patients were included in the DVT group and 295 patients in the Non-DVT group. There was significant difference in age, hypertension, occupation type, malignant tumors, operation time, smoking status, and postoperative exercises between the two groups (p<0.05). However, there was no significant difference in gender, drinking status, coronary heart disease, diabetes, hospital stay, and postoperative hormone or dehydrating agent (p>0.05). In multivariate analysis, age, malignant tumor, hypertension were independent risk factors, while physical labour and postoperative exercises were protective factor for DVT.The postoperative patients with older age, malignant tumor or hypertension should be paid high attention to prevent DVT, and postoperative exercises should be selected as precautionary measures.
Guo C.,Qingdao Municiple Hospital |
Chen G.,The Third Peoples Hospital of Qingdao |
Ge R.,Qingdao University
Molecular Immunology | Year: 2015
Interleukin-23 (IL-23) and IL-17 are involved in the pathogenesis of allergic rhinitis (AR). However, the roles of IL-23 and IL-17 in ovalbumin (OVA)-induced AR remain unclear. Therefore in this study we aim to investigate the precise roles of IL-23 and IL-17 in a mouse model of OVA-induced AR. We found that during OVA-induced AR, eosinophil and goblet cells in the nose were significantly decreased in IL-23-deficient, but not in IL-17-deficient mice. However, there was no difference in the serum IgE and IgG1 levels between IL-23-deficient or IL-17-deficient and wild-type mice. Moreover, IL-4 levels in lymph node cell culture supernatants were significantly decreased in IL-23-deficient, but not IL-17-deficient, compared with wild-type mice. Furthermore, OVA-induced AR developed similarly in wild-type mice transferred with either IL-23-deficient BM cells or wild-type BM cells. These findings suggest that IL-23, but not IL-17 is crucial for the development of OVA-induced AR, and IL-23 neutralization may be a potential approach for treatment of OVA-induced AR in humans. © 2015 Elsevier Ltd.
PubMed | Qingdao University, Qingdao Municiple Hospital and The Third Peoples Hospital of Qingdao
Type: Journal Article | Journal: Molecular immunology | Year: 2015
Interleukin-23 (IL-23) and IL-17 are involved in the pathogenesis of allergic rhinitis (AR). However, the roles of IL-23 and IL-17 in ovalbumin (OVA)-induced AR remain unclear. Therefore in this study we aim to investigate the precise roles of IL-23 and IL-17 in a mouse model of OVA-induced AR. We found that during OVA-induced AR, eosinophil and goblet cells in the nose were significantly decreased in IL-23-deficient, but not in IL-17-deficient mice. However, there was no difference in the serum IgE and IgG1 levels between IL-23-deficient or IL-17-deficient and wild-type mice. Moreover, IL-4 levels in lymph node cell culture supernatants were significantly decreased in IL-23-deficient, but not IL-17-deficient, compared with wild-type mice. Furthermore, OVA-induced AR developed similarly in wild-type mice transferred with either IL-23-deficient BM cells or wild-type BM cells. These findings suggest that IL-23, but not IL-17 is crucial for the development of OVA-induced AR, and IL-23 neutralization may be a potential approach for treatment of OVA-induced AR in humans.
Wang J.-L.,Shandong University |
Wang J.-L.,Qingdao University |
Wu D.-W.,Shandong University |
Cheng Z.-Z.,Qingdao University |
And 3 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014
Objective: This study evaluated the expression level of high mobility group box-B1 (HMGB-1) and matrix metalloproteinase-9 (MMP-9) in non-small cell lung cancer (NSCLC) inmorder to reveal any relation with development and prognosis. Methods: NSCLC and normal tissues were selected from 30 patients at age of 30- 73, and used for RT-PCR and Western blot analyses of HMGB-1. A total of 100 paraffin embedded NSCLC tissues were also isolated from patients through surgical resection, and used for detection of HMGB-1 byimmunohistochemistry. In addition, 50 samples were also applied for MMP-9 detection, and 30 normal tissues were considered as controls. Correlation analysis of HMGB-1 and MMP-9 was carried out by Pearsons correlation coefficient. Results: The average expression level of HMGB-1 in NSCLC patients was significantly higher than in normal lung tissues. In addition, patients in III-IV period exhibit significantly higher positive rate of HMGB- 1 when compared with I-II period cases. Furthermore, a positive correlation with HMGB-1 was found in the expression of MPP-9. Conclusion: HMGB-1 was highly expressed in NSCLC, which may become a prognostic and predictive marker for NSCLC. Besides, MPP-9 was positively correlated with HMGB-1.
PubMed | The Third Peoples Hospital of Qingdao
Type: Comparative Study | Journal: Minerva medica | Year: 2015
The aim of this analysis was to investigate the clinical characteristics and prognosis of patients with serum alpha-fetoproteinpositive gastric cancer (AFPGC) in order to improve the diagnosis and treatment.A retrospective analysis was performed on the clinical characteristics and survival data of patients with gastric cancer in our hospital between March 2007 and September 2012, to compare the clinical characteristics of patients with serum AFPGC to those of patients with serum AFP-negative gastric cancer. A Cox regression model was used to explore the prognosis factors for gastric cancer.The 106 patients with serum AFPGC accounted for 8.5% (106/1253) of all the patients during the same period. There were poorer differentiation (64.2% vs. 54.0%), later clinical stage (83.1% vs. 48.6% at III+IV stage), larger tumor volume (78.3% vs. 57.9% with diameter>5 cm), and higher incidence of liver metastases (14.2% vs. 2.8%) and lymph node metastasis (76.4% vs. 52.7%) in patients with serum AFPGC than in those with serum AFP-negative gastric cancer (P<0.05). The 1-, 3-, and 5-year survival rates in patients with serum AFPGC were 52.8%, 31.3%, and 19.8%, respectively, with a median survival time of 14 months, and those in patients with serum alpha-fetoprotein-negative gastric cancer were 78.3%, 54.8%, and 36.8%, respectively, with a median survival time of 40 months. Multivariate Cox regression analysis showed that serum AFP positive (RR=2.70, 95% CI:1.50~4.87) was one of the risk factors of prognosis for patients with gastric cancer.It is more malignant in patients with serum AFPGC than in those with serum alpha-fetoprotein-negative gastric cancer. There are later clinical stage, poorer differentiation, larger tumor volume, and higher incidence of metastasis to the liver and lymph nodes in patients with serum AFPGC, with low survival rate and poor prognosis.
PubMed | the Third Peoples Hospital of Qingdao
Type: Journal Article | Journal: Bioscience trends | Year: 2015
Oleanolic acid (3-hydroxyolean-12-en-28-oic acid, OA) is a naturally-occurring triterpenoid with various promising pharmacological properties. The present study was conducted to determine the protective effects of OA against oxidized low-density lipoprotein (ox-LDL) induced endothelial cell apoptosis and the possible underlying mechanisms. Our results showed that ox-LDL significantly decreased cell viability and induced apoptosis in human umbilical vein endothelial cells (HUVECs). OA in the co-treatment showed a protective effect against ox-LDL induced loss in cell viability and an increase in apoptosis, which was associated with the modulating effect of OA on ox-LDL induced hypoxia-inducible factor 1(HIF-1) expression. Moreover, our results showed that the modulating effect of OA against ox-LDL induced HIF-1 expression was obtained via inhibition of lipoprotein receptor 1 (LOX-1)/reactive oxygen species (ROS) signaling. Collectively, we suggested that the protective effect of OA against ox-LDL induced HUVEC apoptosis might, at least in part, be obtained via inhibition of the LOX-1/ROS/HIF-1 signaling pathway.
PubMed | Qingdao University and The Third Peoples Hospital of Qingdao
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2017
The aim of the present study was to observe the long-term prognostic value of early interventional therapy for lower extremity deep venous thrombosis (LDVT). In total, 85 patients diagnosed with LDVT for the first time were consecutively selected (identified course of disease was <3 months), and were divided into the control group with 43 cases and the observation group with 42 cases according to different therapeutic methods. The control group received anticoagulation therapy and thrombolysis, or integrated surgical thrombectomy, a conventional open operation, while the observation group received comprehensive treatment, combining endovascular catheter-directed thrombolysis and thrombectomy. The therapeutic effects were compared. After treatment, the differences in circumference of the thigh and shank between the affected and unaffected extremities, and vein dysfunction score of the two groups were decreased compared with before treatment. In addition, the above indexes of the observation group were significantly lower than in the control group (P<0.05). The clinical effective rate and effective extent of the observation group were higher than those of the control group, and the differences were statistically significant (P<0.05). The occurrence rate of post-thrombotic syndrome in the observation group was lower than that of the control group, and there was no difference in comparison of grading. The recurrence rate and restenosis rate of the observation group were lower than in the control group, while the patency rate of the observation group was higher than that of the control group, and the differences were statistically significant (P<0.05). In conclusion, early catheter-directed invention of thrombolysis with thrombectomy for LDVT has good clinical effect in the short-term and long-term.