Cui J.,Shandong University |
Lin A.L.,The Third Peoples Hospital of Hangzhou City |
Liu Q.,Shandong University |
Sun Q.,Shandong University |
Gao Z.-H.,University of Calgary
International Journal of Immunogenetics | Year: 2010
Dendritic cells (DC) are professional antigen-presenting cells that can actively taken up and present tumour-derived proteins to induce a tumour-specific immune response. Granulocyte-macrophage colony-stimulating factor (GM-CSF) plays a pivotal role in the generation, sensitization, maturation and survival of DC. We charged the peripheral blood monocyte cell-derived DC with tumour lysate, and then transfected the DC with lentiviral vector-encoding human GM-CSF (hGMCSF). The antigen-presenting capacity of the hGM-CSF-transfected DC was tested by means of the mixed lymphocyte reaction and cytotoxic T-lymphocyte assay using wild-type DC as the control. The Lenti-hGM-CSF-transfected DC was able to stimulate the proliferation of naive allogeneic T lymphocytes and to generate tumour-specific cytotoxic T lymphocytes more efficiently than the wild-type DC. This data indicates that Lenti-hGMCSF-transfected DC could potentially be used as an effective clinical approach for cancer immunotherapy. © 2010 Blackwell Publishing Ltd.