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Fang X.,The Third Peoples Hospital of Dalian | Fang X.,Dalian Medical University | Tao D.,Dalian Medical University | Shen J.,Dalian Medical University | And 3 more authors.
Neuroscience Letters | Year: 2015

Atorvastatin has been reported to ameliorate ischemic brain damage after stroke, but the underlying mechanisms are not clear. This study investigated the effect of atorvastatin on dynamic expressions of MMP9 and TIMP1 in rats after cerebral ischemia reperfusion (I/R). Atorvastatin (5 mgkg-1d-1) or vehicle was administered orally to rats for 21d before middle cerebral artery occlusion (MCAo) for 2h, with perfusion at 3-, 12-, 24-, 48-, or 96-h thereafter. To evaluate functional outcome, a 5-point behavioral rating scale was performed. Ischemic lesion volume was assessed via triphenyl tetrazolium chloride (TTC) staining. mRNA levels of MMP-9 and TIMP-1 were detected by reverse transcription-PCR, and protein levels of MMP-9 and TIMP-1 were measured by immunohistochemical SABC method. At all reperfusion time points, atorvastatin pretreatment was associated with significantly (P<0.05) improved neurological function and reduced brain infarct sizes compared with vehicle treatment, and MMP9 levels were significantly (P<0.05) lower and TIMP1 levels were significantly (P<0.05) higher in both mRNA and protein levels. In conclusion, Oral administration of atorvastatin before stroke may reduce the severity in I/R injury and improve neurological outcome by lowering MMP9 levels and elevating TIMP1 levels. © 2015 Elsevier Ireland Ltd. Source


Gao L.,Capital Medical University | Liu Y.,Dalian Municipal Friendship Hospital | Li X.,Dalian Municipal Friendship Hospital | Bai Q.,Dalian Municipal Friendship Hospital | Liu P.,The Third Peoples Hospital of Dalian
Archives of Gerontology and Geriatrics | Year: 2015

Objective: We investigated possible abnormalities in the retinal nerve fiber layer (RNFL) and macula lutea of patients diagnosed with Alzheimer's disease (AD) and mild cognitive impairment (MCI) and tested for any correlation with the severity of dementia. Methods: A total of 72 subjects, comprising 25 AD patients, 26 MCI patients and 21 healthy individuals (controls) were enrolled in this study. The thickness of the RNFL and volume of the macula lutea was determined using optical coherence tomography (OCT). Results: When compared with controls, we found statistically significant thinning of the RNFL in AD patients at all clock-hour positions except 12:00, and nasal quadrant, 2:00, 3:00 and 4:00. After adjusting several risk factors, the average thickness of the RNFL was reduced in MCI patients compared to AD patients, with specific reductions at inferior quadrant, 5:00 and 6:00. Compared to controls, MCI patients showed a significant decrease in RNFL thickness only in the temporal quadrant, 8:00, 9:00 and 10:00. We found significant reduction in the volume of the macula lutea both in AD and MCI patients. Finally, we could not establish any correlation between patient Mini-Mental State Examination (MMSE) scores (an estimation of the severity of cognitive impairment) and any OCT parameter. Conclusion: Retinal degeneration in AD and MCI patients results in decreased thickness of the RNFL, and reduced macular volume in AD and MCI patients. However, there seems to be no correlation between these changes and the severity of dementia. © 2014 Elsevier Ireland Ltd. Source


Cui L.,The Third Peoples Hospital of Dalian | Ma X.,Dalian Medical University | Zhao Y.-H.,Dalian Armed Police Hospital
International Eye Science | Year: 2014

AIM: To explore the feasibility of culturing human umbilical vein endothelial cells (HUVEC) on acellular corneal stroma and performing the posterior lamellar endothelial keratoplasty (PLEK) treating corneal endothelial decompensation. METHODS: Thirty New-Zealand rabbits were divided into three groups randomly, 10 rabbits for experimental group, 10 for stroma group and 10 for control group. Corneal endothelial cells were removed to establish animal model of corneal endothelial failure. PLEK was performed on the rabbits of experimental group and stroma group, and nothing was transplantated onto the rabbits of control group with the deep layer excised only. Postoperative observation was taken for 3 mo. The degree of corneal edema and central corneal thickness were recorded for statistical analysis. RESULTS: Corneas in experimental group were relieved in edema obviously compared with that in stroma group and the control group, and showed increased transparency 7d after the operation. The average density of endothelial cells was 2 026.4±129.3cells/mm2, and average central corneal thickness was 505.2±25.4μm in experimental group, while 1535.6±114.5μm in stroma group and 1 493.5±70.2μm in control group 3mo after operation. CONCLUSION: We achieved preliminary success in our study that culturing HUVEC on acellular corneal stroma and performing PLEK for corneal endothelial decompensation. HUVEC transplanted could survive in vivo, and have normal biological function of keeping cornea transparent. This study provides a new idea and a new way clinically for the treatment of corneal endothelial diseases. Source


Zhou S.-Y.,Dalian Medical University | Tong L.,Dalian Medical University | Song F.,Dalian Medical University | Hong X.-J.,Dalian Medical University | And 5 more authors.
Epilepsy Research | Year: 2015

Background: Different subtypes of idiopathic generalized epilepsy may indicate different mechanisms and outcomes, suggesting that it is necessary to use a 'pure sample' of a single subtype. Methods: A volumetric study, in conjunction with cognition assessments, was performed in a pure sample of patients with idiopathic generalized tonic-clonic seizures (IGE-GTCS; 15 males and 15 females) matched with normal control subjects (15 males and 17 females). The volumetric measurements were focused on the hippocampus and its surrounding structures, including the amygdala, the parahippocampal gyrus, the entorhinal cortex and the perirhinal cortex. The Wechsler Adult Intelligence Scale-Revised in China was administered to assess cognitive status. Results: A volume reduction was found only in the hippocampus, with a more severe effect on the left side than the right side. The total number and frequency of seizures had significant negative correlations with multiple cognitive measures. Furthermore, the hippocampal volume reduction was significantly correlated with some aspects of cognitive impairment. Conclusion: These findings suggest that compared with the other medial temporal structures, the hippocampus may be more vulnerable to the neuropathology of IGE-GTCS. The observation that cognitive deterioration was correlated with an increased frequency and total number of seizures highlights the critical importance of preventing seizures from recurrence. © 2014 Elsevier B.V. Source


Zhao L.,The Third Peoples Hospital of Dalian | Zhang F.-Y.,The Third Peoples Hospital of Dalian
International Journal of Ophthalmology | Year: 2011

AIM: To establish a method for rapid detection of clinical suspect fungal corneal ulcer by reverse transcription-polymerase chain reaction(RT-PCR). METHODS: A pair of oligonucleotide sequences, which was based on the conserved region of 18srRNA shared by medically important conditioned fungi, was used as the general primers to amplify the DNAs from clinical suspect fungal corneal ulcer in a RT-PCR assay, and the result was compared with culture. RESULTS: A 400bp specific DNA product was successfully amplified. The positive rate of fungi culture among 26 clinical specimens was 61.9%, while that of RT-PCR amplification was 73.8%. In addition, the accuracy of PCR method in this study was 83.8%, the sensitivity was 100.0%, and the specialty was 33.3%. CONCLUSION: PCR with the general primers is suitable for rapid detection of fungal corneal ulcer because of quickness and high positive rate. Source

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