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Liu L.,Chongqing Medical University | Fu R.,Huazhong University of Science and Technology | Yuan X.,Huazhong University of Science and Technology | Shi C.,Huazhong University of Science and Technology | And 6 more authors.
Current Microbiology | Year: 2015

Screening live mycobacterial vaccine candidates is the important strategy to develop new vaccines against adult tuberculosis (TB). In this study, the immunogenicity and protective efficacy of several avirulent mycobacterial strains including Mycobacterium smegmatis, M. vaccae, M. terrae, M. phlei, M. trivial, and M. tuberculosis H37Ra were compared with M. bovis BCG in BALB/c mice. Our results demonstrated that differential immune responses were induced in different mycobacterial species vaccinated mice. As BCG-vaccinated mice did, M. terrae immunization resulted in Th1-type responses in the lung, as well as splenocytes secreting IFN-γ against a highly conserved mycobacterial antigen Ag85A. M. smegmatis also induced the same splenocytes secreting IFN-γ as BCG and M. terrae did. In addition, M. terrae and M. smegmatis-immunized mice predominantly increased expression of IL-10 and TGF-β in the lung. Most importantly, mice vaccinated with H37Ra and M. vaccae could provide the same protection in the lung against virulent M. tuberculosis challenge as BCG. The result may have important implications in developing adult TB vaccine. © 2015, Springer Science+Business Media New York.

PubMed | The Third Peoples Hospital of Chongqing City and Luzhou Medical College
Type: Journal Article | Journal: Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus | Year: 2015

To evaluate the efficacy of topical atropine 1% in promoting unaided visual acuity, reducing myopia, and slowing the progression of ocular axial elongation in Chinese children with low myopia.Children with low myopia were randomly assigned to one of two groups, receiving either atropine 1% (treatment group) or placebo eyedrops (control group) once nightly for 1 year. After instillation of 3 drops of cyclopentolate 1%, unaided visual acuity, cycloplegic refraction, and ocular axial length were tested and recorded at baseline (2 weeks after atropine or vehicle eyedrops), 3 months, 6 months, 9 months, and 1 year.A total of 132 children 7-12 years of age with a refractive error of spherical equivalent -0.50 D to -2.00 were included. After 1 year, the mean unaided visual acuity in the treatment group was 0.31 0.16 logMAR; in the control group, 0.66 0.15 logMAR, (P < 0.0001). After treatment for 1 year, there was a decrease of 0.32 0.22 D from baseline in the treatment group and an increase of -0.85 0.31 D in the control group (P < 0.0001). The axial elongation in the treatment group was -0.03 0.07 mm; in the control group, 0.32 0.15 mm (P < 0.0001).In this study cohort, topical atropine1% reduced the degree of low myopia and slowed the progression of ocular axial elongation in children.

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