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Chen Y.,The Ninth Peoples Hospital of Chongqing | Huang F.,The Third Peoples Hospital of Chongqing | Wang D.,The Ninth Peoples Hospital of Chongqing | Weng Z.,The Ninth Peoples Hospital of Chongqing | Deng Z.,Chongqing Medical University
Experimental and Therapeutic Medicine

Heme oxygenase (HO)-1 is highly expressed in the hippocampus. Its expression is induced by many factors including hemes, whose metabolites play an important role in neuron protection and learning development. In the present study, the correlation between HO-1 and learning ability was investigated in mice. Behavioral tests were used to evaluate the effects of altering HO-1 on learning ability in mature mice. In order to determine the function of HO-1 in the immature mice, a dark-reared model was constructed. Either the HO-1 inducer hemin or the HO-1 inhibitor Zn protoporphyrin IX (ZnPPIX) was injected into the left lateral ventricle prior to a behavior test. Results showed that neither hemin nor ZnPPIX affected the learning ability of adult mice reared in normal conditions. The hippocampal HO-1 of dark-reared mice was decreased while it was increased in the behavioral training group. In general, HO-1 had no effect on established learning ability but it may be upregulated by behavioral training and is beneficial for the development of memory and learning ability in neonatal mice. Source

Zhang J.,The Third Peoples Hospital of Chongqing | Liu X.,The Third Peoples Hospital of Chongqing | Li X.-J.,Chongqing Medical University
Genetics and Molecular Research

The phages of Acinetobacter baumannii has drawn increasing attention because of the multi-drug resistance of A. baumanni. The aim of this study was to sequence Acinetobacter baumannii phage AB3 and conduct bioinformatic analysis to lay a foundation for genome remodeling and phage therapy. We isolated and sequenced A. baumannii phage AB3 and attempted to annotate and analyze its genome. The results showed that the genome is a double-stranded DNA with a total length of 31,185 base pairs (bp) and 97 open reading frames greater than 100 bp. The genome includes 28 predicted genes, of which 24 are homologous to phage AB1. The entire coding sequence is located on the negative strand, representing 90.8% of the total length. The G+C mol% was 39.18%, without areas of high G+C content over 200 bp in length. No GC island, tRNA gene, or repeated sequence was identified. Gene lengths were 120-3099 bp, with an average of 1011 bp. Six genes were found to be greater than 2000 bp in length. Genomic alignment and phylogenetic analysis of the RNA polymerase gene showed that similar to phage AB1, phage AB3 is a phiKMV-like virus in the T7 phage family. © FUNPEC-RP. Source

Pei W.-X.,The Third Peoples Hospital of Chongqing
International Eye Science

AIM: To evaluate the therapeutic efficacy and safety of trabeculectomy for primary acute angle-closure glaucoma(PACG) with high intraocular pressure (IOP). METHODS: Fourty-eight cases (50 eyes) of PACG were retrospectively analyzed, which underwent trabeculectomy. These cases were divided into 2 groups by the preoperative IOP: the operation group (12 eyes, IOP≥40mmHg), and the control group (38 eyes, IOP<40mmHg). The IOP, visual acuity, complications and surgery outcome of both groups were compared. RESULTS: No severe complications such as choroidal hemorrhage, malignant glaucoma were observed in both groups during surgery and postoperation. The success rate of operation in the control group was much higher than that in the operation group shortly after the operation with significant difference (P=0.0332). But the success rate of both groups had no significant difference(P>0.05) in return visit. CONCLUSION: Trabeculectomy for PACG with persistent high IOP should be performed actively to avoid further damage to the visual function. Source

Tu X.H.,The Third Peoples Hospital of Chongqing
Zhongguo zhen jiu = Chinese acupuncture & moxibustion

To explore the early rehabilitation effect of acupuncture on brain arousal in severe craniocerebral injury. One hundred and two cases of severe craniocerebral injury were randomly divided into an observation group and a control group, 51 cases in each one. Based on the conventional nursing care in neurological external medicine, in observation group, acupuncture was applied at Shuigou (GV 26), Neiguan (PC 6) and Sanyinjiao (SP 6) mainly. In control group, functional electric stimulation was applied at stimulate the affected muscles of the upper limbs. Thirty days later, the lucid rate from coma, lucid interval and clinical efficacy were compared between two groups. RESULTS; The lucid rate from coma was 82.4% (42/51) in observation group, which was higher than 56.9% (29/51) in control group (P < 0.01). The lucid interval in observation group was shortened remarkably as compared with control group (P < 0.01), and the clinical efficacy was superior apparently to that in control group (P < 0.01). On the basis of conventional treatment, acupuncture intervention at early stage can accelerate the recovery of brain arousal function in patients with severe craniocerebral injury. Source

Zhang J.,The Third Peoples Hospital of Chongqing
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences

To investigate the biological characteristics of phage SM1 for stenotrophomonas maltophilia (Sm) and its effect in animal infection model. Phage SM1 isolated from raw sewage of hospital was identified by the plaque method. The morphology of phage SM1 was observed by electron micrographics with negative staining. The extraction and electrophoresis of phage SM1 DNA were performed. Optimal multiplicity of infection, resistant mutation rate, one step growth curve and the effectiveness in animal models of phage SM1 were determined. One Sm specific phage of myoviridae double-stranded DNA was identified, and named SM1. Electrophoresis of DNA demonstrated that the size of phage SM1 genome was about 50 kb. The growth curve of phage SM1 showed that the durations of incubation and burst period were 15 min and 50 min, respectively; and the burst size was 187. The resistant mutation rate of phage SM1 was 6 x 10(-10). All mice treated with phage SM1 survived after 7 d of infection with stenotrophomonas maltophilia. The phage SM1 has a relatively broad host range, a shorter incubation period,an apparent burst size and a lower resistant mutation rate. The therapy of phage SM1 for Sm infection in mice is effective. Source

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