Entity

Time filter

Source Type


Xiao J.,The Third Institute of Oceanography SOA | Xiao J.,University of Tsukuba | Luo Y.,The Third Institute of Oceanography SOA | Xu J.,The Third Institute of Oceanography SOA | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2011

The taxonomic status of an actinobacterium that changed colour during growth, strain 42H12-1 T, isolated from deep-sea sediment collected from the Atlantic Ocean, was established using a combination of genotypic and phenotypic data. Strain 42H12-1 T formed a distinct branch in the 16S rRNA gene phylogenetic tree together with the type strains in the genus Modestobacter. The highest sequence similarity by BLAST analysis was to Modestobacter versicolor CP153-2 T (98.5 %) and the second-highest sequence similarity was to Modestobacter multiseptatus AA-826 T (97.5 %). DNA-DNA relatedness of only 12% (SD 1.82 %) between strain 42H12-1 T and M. versicolor DSM 16678 T differentiated them as members of separate genomic species. Colonies of strain 42H12-1 T were black on oligotrophic medium, but orange to red, turning black, on copiotrophic medium. The peptidoglycan contained meso-diaminopimelic acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and an unknown aminophospholipid. The predominant menaquinone was MK-9(H 4). The major fatty acids were iso-C 16: 0 and C 17: 1ω8c. The DNA G+C content was 72.3±1 mol%. Strain 42H12-1 T (=DSM 45201 T =CGMCC 4.5581 T) is assigned as the type strain of a novel species of the genus Modestobacter, for which the name Modestobacter marinus sp. nov. is proposed. © 2011 IUMS. Source


Luo Y.,The Third Institute of Oceanography SOA | Xiao J.,The Third Institute of Oceanography SOA | Xiao J.,University of Tsukuba | Wang Y.,The Third Institute of Oceanography SOA | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2011

The taxonomic position of an actinomycete isolated from deep-sea sediment from the Indian Ocean was determined by using a polyphasic approach. The presence of iso-C 15:0, anteiso-C 15:0, iso-C 16:0, iso-C 17:0 and anteiso-C 17:0 as the major cellular fatty acids, LL-diaminopimelic acid as the characteristic diamino acid, and MK-9(H 4, H 6 and H 8) as the major menaquinones supported the affiliation of strain IH32-1 T to the genus Streptomyces. Comparison of 16S rRNA gene sequences showed that strain IH32-1 T exhibited highest similarities to the type strains of Streptomyces globosus (97.6%) and Streptomyces toxytricini (97.6%). However, DNA-DNA relatedness values between strain IH32-1 T and the type strains of S. globosus and S. toxytricini were determined as 55.2±4.7 and 38.3±2.5%, respectively. Based on its chemotaxonomic, phenotypic and genotypic characteristics, strain IH32-1 T is considered to represent a novel species in the genus Streptomyces, for which the name Streptomyces indicus sp. nov. is proposed. The type strain is IH32-1 T (=DSM 42001 T =CGMCC 4.5727 T). © 2011 IUMS. Source


Zhang X.,The Third Institute of Oceanography SOA | Jiang L.,The Third Institute of Oceanography SOA | Alain K.,CNRS Lab for Microbiology of Extreme Environments | Jebbar M.,CNRS Lab for Microbiology of Extreme Environments | Shao Z.,The Third Institute of Oceanography SOA
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A hyperthermophilic, anaerobic, piezophilic archaeon (strain DY20341T) was isolated from a sediment sample collected from an East Pacific Ocean hydrothermal field (1° 37' S 102° 45' w) at a depth of 2737 m. The cells were irregular cocci, 0.8-1.5 mm in diameter. Growth was observed between 50 and 90 °C (optimum 80 ° C), pH 5.0 and 8.0 (optimum pH 7.0), 1% and 7% (w/v) sea salts (Sigma, optimum 3 %), 1% and 4% (w/v) NaCl (optimum 3%) and 0.1 and 80 MPa (optimum 30 MPa). The minimum doubling time was 66 min at 30 MPa and 80 ° C. The isolate was an obligate chemoorganoheterotroph, capable of utilizing complex organic compounds and organic acids including yeast extract, peptone, tryptone, casein, starch, Casamino acids, citrate, lactate, acetate, fumarate, propanoate and pyruvate for growth. It was strictly anaerobic and facultatively dependent on elemental sulfur or sulfate as electron acceptors, but did not reduce sulfite, thiosulfate, Fe(III) or nitrate. The presence of elemental sulfur enhanced growth. The G+C content of the genomic DNA was 43.6±1 mol%. 16S rRNA gene sequence analysis revealed that the closest relative of the isolated organism was Palaeococcus ferrophilus DMJT (95.7% 16S rRNA gene similarity). On the basis of its physiological properties and phylogenetic analyses, the isolate is considered to represent a novel species, for which the name Palaeococcus pacificus sp. nov. is proposed. The type strain is strain DY20341T (5JCM 17873T5DSM 24777T). © 2013 IUMS. Source


Zeng X.,The Third Institute of Oceanography SOA | Zeng X.,Key Laboratory of Marine Genetic Resources of Fujian Province | Zeng X.,Xiamen State Key Laboratory Breeding Base of Marine Genetic Resources | Zhang Z.,The Third Institute of Oceanography SOA | And 17 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

A novel piezophilic, thermophilic, anaerobic, fermentative bacterial strain, designated strain DY22613T, was isolated from a deep-sea hydrothermal sulfide deposit at the East Pacific Rise (GPS position: 102.66 W 3.16 S). Cells of strain DY22613T were long, motile rods (10 to 20 mm in length and 0.5 mm in width) with peritrichous flagella and were Gram-stain-negative. Growth was recorded at 44–72 6C (optimum 60–62 6C) and at hydrostatic pressures of 0.1–55 MPa (optimum 20 MPa). The pHrange for growth was frompH 5.0 to 9.0 with an optimum at pH 7.0. Growth was observed in the presence of 1 to 8% (w/v) sea salts and 0.65 to 5.2% (w/v) NaCl, with optimum salt concentrations at 3.5% for sea salts and at 2.3% for NaCl. Under optimal growth conditions, the shortest generation time observed was 27 min (60 6C, 20 MPa). Strain DY22613T was heterotrophic, able to utilize complex organic compounds, amino acids, sugars and organic acids including peptone, tryptone, beef extract, yeast extract, alanine, glutamine, methionine, phenylalanine, serine, threonine, fructose, fucose, galactose, gentiobiose, glucose, mannose, melibiose, palatinose, rhamnose, turanose, pyruvate, lactic acid, methyl ester, erythritol, galacturonic acid and glucosaminic acid. Strain DY22613T was able to reduce Fe(III) compounds, including Fe(III) oxyhydroxide (pH 7.0), amorphous iron(III) oxide (pH 9.0), goethite (a-FeOOH, pH 12.0), Fe(III) citrate and elementary sulfur. Products of fermentation were butyrate, acetate and hydrogen. Main cellular fatty acids were iso-C15:0, iso-C14:0 3-OH and C14:0. The genomic DNA G+C content of strain DY22613T was 36.7 mol%. Based on 16S rRNA gene sequence analysis, the strain forms a novel lineage within the class Clostridia and clusters with the order Haloanaerobiales (86.92% 16S rRNA gene sequence similarity). The phylogenetic data suggest that the lineage represents at least a novel genus and species, for which the name Anoxybacter fermentans gen. nov., sp. nov. is proposed. The type strain is DY22613T (5JCM 19466T5DSM 28033T5MCCC 1A06456T). ©2015 IUMS. Source

Discover hidden collaborations