The Third Hospital of Wuhan
The Third Hospital of Wuhan
Maixner D.W.,University of Georgia |
Yan X.,University of Georgia |
Yan X.,The Third Hospital of Wuhan |
Yan X.,Tongji University |
And 2 more authors.
Neuroscience | Year: 2016
Emerging studies have shown that pharmacological activation of adenosine monophosphate-activated protein kinase (AMPK) produces potent analgesic effects in different animal pain models. Currently, the spinal molecular and synaptic mechanism by which AMPK regulates the pain signaling system remains unclear. To address this issue, we utilized the Cre-LoxP system to conditionally knockout the AMPKα1 gene in the nervous system of mice. We demonstrated that AMPKα1 is imperative for maintaining normal nociception, and mice deficient for AMPKα1 exhibit mechanical allodynia. This is concomitantly associated with increased glutamatergic synaptic activities in neurons located in the superficial spinal dorsal horn, which results from the increased glutamate release from presynaptic terminals and function of ligand-gated glutamate receptors at the postsynaptic neurons. Additionally, AMPKα1 knockout mice have increased activities of extracellular signal-regulated kinases (ERK) and p38 mitogen-activated protein kinases (p38), as well as elevated levels of interleukin-1β (IL-1β), reactive oxygen species (ROS), and heme oxygenase 1 (HO-1) in the spinal dorsal horn. Systemic administration of a non-specific ROS scavenger (phenyl-N-tert-butylnitrone, PBN) or a HO-1 activator (Cobalt protoporphyrin IX, CoPP) attenuated allodynia in AMPKα1 knockout mice. Bath-perfusion of the ROS scavenger or HO-1 activator effectively attenuated the increased ROS levels and glutamatergic synaptic activities in the spinal dorsal horn. Our findings suggest that ROS are the key down-stream signaling molecules mediating the behavioral hypersensitivity in AMPKα1 knockout mice. Thus, targeting AMPKα1 may represent an effective approach for the treatment of pathological pain conditions associated with neuroinflammation at the spinal dorsal horn. © 2016.
Xia Y.,The Third Hospital of Wuhan
Medical Journal of Wuhan University | Year: 2017
Objective: To explore and analyze the volume kinetics of elderly patients under epidural block and general anesthesia with intravenous injection of Ringer's lactate solution. Methods: Fifty-two cases of elderly patients undergoing selective upper abdominal surgery between August 2013 and August 2014 were enrolled into our study and randomly divided into two groups with 26 cases in each group. Patients in A group were conducted epidural anesthesia 10 minutes after Ringer's lactate solution was intravenously injected at a constant speed, while patients in B group were given general anesthesia 20 minutes after Ringer's lactate solution injection. The volume kinetics was compared and analyzed between these two groups. Results: The heart rate (HR) was not foundsignificant changein the two groups at each time point, while MAP of group B decreased remarkablely at T30 and T60, with (91.1±18.0)mmHg and (92.8±17.9)mmHg, respectively, which were significantly lower than those of group A with (104.9±17.1)mmHg and (102.7±17.2)mmHg(P<0.05). The plasma dilutions of group B at T30(0.17±0.05) and T60(0.30±0.09) were significantly higher than those of group A with (0.12±0.05) and (0.18±0.10). Blood volume expansion efficiency of group B at T30 with (4.6±1.4)ml/kg and T60 with (7.7±2.9)ml/kg were also greatly higher than those in group A of (3.0±1.2)ml/kg and (4.6±2.7)ml/kg. Moreover, the increase of blood volume per unit of body mass as (0.51±0.15)% and (0.44±0.18)% at T30 and T60 in the group A were also obviously more than group A with (0.39±0.14)% and (0.31±0.16)%. All those above differences were statistically significant (P<0.05). Conclusion: Under general anesthesia, the volume kinetics changes of elderly patients are more obvious with intravenous injection of Ringer's lactate solution. © 2017, Editorial Board of Medical Journal of Wuhan University. All right reserved.
Lei X.,Renmin University of China |
Lv X.,Renmin University of China |
Liu M.,The Third Hospital of Wuhan |
Yang Z.,Renmin University of China |
And 3 more authors.
Biochemical and Biophysical Research Communications | Year: 2012
Thymoquinone (TQ), a component derived from the bioactive constituent of black seed (Nigella sativa), has been shown to exert biological activity on various types of human cancers. However, there are few studies addressing its effects on gastric cancer. Here, we present the first report describing the chemosensitizing effect of thymoquinone and 5-fluorouracil (5-FU) on gastric cancer cells both in vitro and in vivo. Studies have shown that pretreatment with TQ significantly increased the apoptotic effects induced by 5-FU in gastric cancer cell lines in vitro. Moreover, we found that TQ enhanced the 5-FU-induced killing of gastric cancer cells by mediating the downregulation of the anti-apoptotic protein bcl-2, the upregulation of the pro-apoptotic protein bax, and the activation of both caspase-3 and caspase-9. In addition to the in vitro results, it has been shown that the combined treatment of TQ with 5-FU represents a significantly more effective antitumor agent than either agent alone in a xenograft tumor mouse model. These data suggest that the TQ/5-FU combined treatment induces apoptosis by enhancing the activation of both caspase-3 and caspase-9 in gastric cancer cells. These results, which provide molecular evidence both in vitro and in vivo, support our conclusion that thymoquinone can activate caspase-3 and caspase-9 and thus result in the chemosensitisation of gastric cancer cells to 5-FU-induced cell death. © 2011 Elsevier Inc.
Wei M.,The Third Hospital of Wuhan
Medical Journal of Wuhan University | Year: 2016
Objective: To improve the effect and safety observation of donepezil and memantine in treatment of Alzheimer disease patients cognitive function and behavior ability. Methods: Seventy cases of senile patients with dementia were selected in our hospital, according to the different treatment plans, they were divided into the study group and the control group, the study group was treated with donepezil and memantine, the control group only received donepezil. The different conditions of patients before and after treatment were compared by using mini mental state examination (MMSE), Alzheimer disease assessment scale cognitive subscale (ADAS-Cog), neurology and psychiatry questionnaire (NPI), activity of daily living scale (ADL). Results: MMSE, ADAS-Cog, NPI and ADL scores of the study group and the control group after treatment were significantly better than those before treatment, the difference was significant (P<0.05); After treatment, ADAS-Cog and NPI scores in patients of the study group were (18.37±5.35) and (24.96±3.41) and ADAS-Cog and NPI scores of the control group of patients were (26.17±5.32) and (23.78±3.20), the difference was significant (P<0.05). Conclusion: The clinical curative effect of donepezil with memantine in treatment of the patients with senile dementia is precise, and can effectively improve the cognitive function of patients and behavior ability, which is better than simple use of donepezil, it is worthy of promotion. © 2016, Editorial Board of Medical Journal of Wuhan University. All right reserved.
Zhou T.,The Third Hospital of Wuhan
Zhonghua nan ke xue = National journal of andrology | Year: 2012
To explore the effect of anhydrous alcohol sclerotherapy following transrectal ultrasound-guided puncture in the treatment of Mülllerian duct cyst. Totally 44 patients with Müllerian duct cyst underwent transrectal ultrasound-guided puncture and sclerotherapy, 21 injected with anhydrous alcohol of half the volume of the aspirated cystic fluid followed by aspiration of all the fluid 5 minutes later (treatment group) , and the other 23 treated by cystic fluid aspiration only (control group). The clinical effects of the two methods were compared. The response rate and cure rate were 80.95 and 52.38% in the treatment group, as compared with 56.52 and 26.09% in the control (P < 0.001). No adverse events were observed in either of the two groups. Transrectal ultrasound-guided puncture and anhydrous alcohol sclerotherapy is a safe and effective approach to the treatment of Mülllerian duct cyst.
Yin Q.,The Third Hospital of Wuhan |
Xia Y.,The Third Hospital of Wuhan |
Wang G.,Guangzhou University
Biochemical and Biophysical Research Communications | Year: 2016
As an early sign of diabetic cardiovascular disease, endothelial dysfunction may contribute to progressive diabetic nephropathy (DN). Endothelial hyperpermeability induced by hyperglycemia (HG) is a central pathogenesis for DN. Sinomenine (SIN) has strong anti-inflammatory and renal protective effects, following an unknown protective mechanism against HG-induced hyperpermeability. We herein explored the role of SIN in vitro in an HG-induced barrier dysfunction model in human renal glomerular endothelial cells (HRGECs). The cells were exposed to SIN and/or HG for 24 h, the permeability of which was significantly increased by HG. Moreover, junction protein occludin in the cell-cell junction area and its total expression in HRGECs were significantly decreased by HG. However, the dysfunction of tight junction and hyperpermeability of HRGECs were significantly reversed by SIN. Furthermore, SIN prevented HG-increased reactive oxygen species (ROS) by activating nuclear factor-E2-related factor 2 (Nrf2). Interestingly, activation of RhoA/ROCK induced by HG was reversed by SIN or ROCK inhibitor. HG-induced hyperpermeability was prevented by SIN. High ROS level, tight junction dysfunction and RhoA/ROCK activation were significantly attenuated with knockdown of Nrf2. Mediated by activation of Nrf2, SIN managed to significantly prevent HG-disrupted renal endothelial barrier function by suppressing the RhoA/ROCK signaling pathway through reducing ROS. We successfully identified a novel pathway via which SIN exerted antioxidative and renal protective functions, and provided a molecular basis for potential SIN applications in treating DN vascular disorders. © 2016 Elsevier Inc.
Chen H.,Huazhong University of Science and Technology |
Li J.,Wuhan University |
Jiao L.,Huazhong University of Science and Technology |
Petersen R.B.,Case Western Reserve University |
And 4 more authors.
Journal of Physiology | Year: 2014
Diabetic nephropathy is the primary cause of end-stage renal disease. Increasing numbers of patients are suffering from this disease and therefore novel medications and therapeutic approaches are urgently needed. Here, we investigated whether apelin-13, the most active member of the adipokine apelin group, could effectively suppress the development of nephropathy in Akita mouse, a spontaneous type 1 diabetic model. Apelin-13 treatment decreased diabetes-induced glomerular filtration rate, proteinuria, glomerular hypertrophy, mesangial expansion and renal inflammation. The inflammatory factors, activation of NF-κB, histone acetylation and the enzymes involved in histone acetylation were further examined in diabetic kidneys and high glucose- or sodium butyrate-treated mesangial cells in the presence or absence of apelin-13. Apelin-13 treatment inhibited diabetes-, high glucose- and NaB-induced elevation of inflammatory factors, and histone hyperacetylation by upregulation of histone deacetylase 1. Furthermore, overexpression of apelin in mesangial cells induced histone deacetylation under high glucose condition. Thus, apelin-13 may be a novel therapeutic candidate for treatment of diabetic nephropathy via regulation of histone acetylation. © 2013 The Physiological Society.
Yan X.,University of Georgia |
Yan X.,The Third Hospital of Wuhan |
Yadav R.,University of Georgia |
Gao M.,University of Georgia |
Weng H.-R.,University of Georgia
GLIA | Year: 2014
Excessive activation of glutamate receptors in spinal dorsal horn neurons is a key mechanism leading to abnormal neuronal activation in pathological pain conditions. Previous studies have shown that activation of glutamate receptors in the spinal dorsal horn is enhanced by impaired glial glutamate transporter functions and proinflammatory cytokines including interleukin-1 beta (IL-1β). In this study, we for the first time revealed that spinal glial glutamate transporter activities in the neuropathic animals are attenuated by endogenous IL-1β. Specifically, we demonstrated that nerve injury results in an increased expression of IL-1β and activation of PKC in the spinal dorsal horn as well as suppression of glial glutamate uptake activities. We provided evidence that the nerve-injury induced suppression of glial glutamate uptake is at least in part ascribed to endogenous IL-1β and activation of PKC in the spinal dorsal horn. IL-1β reduces glial glutamate transporter activities through enhancing the endocytosis of both GLT-1 and GLAST glial glutamate transporters. The IL-1β induced trafficking of glial glutamate transporters is through the calcium/PKC signaling pathway, and the dynamin-dependent endocytosis, which is dependent on the integrity of actin filaments. The signaling pathway regulating glial glutamate transporters revealed in this study provides novel targets to attenuate aberrant activation of glutamate receptors in the spinal dorsal horn, which could ultimately help the development of analgesics. © 2014 Wiley Periodicals, Inc.
Luo C.F.,The Third Hospital of Wuhan
Zhonghua zhong liu za zhi [Chinese journal of oncology] | Year: 2010
To investigate the expressions of bFGF and PTEN in cervical carcinoma and their clinical significance. Tissue microarray technique and immunohistochemistry SP method were used to detect the expressions of bFGF and PTEN in 143 cases of invasive carcinoma of cervix (ICC) and 20 cases of normal cervical epithelium remote from tumor (NCE). The relationship between the expressions of bFGF and PTEN in ICC and some factors relating to clinical pathology of cervical carcinoma such as histopathological grading, lymph node metastasis, stroma involvement and FIGO staging were analyzed. The rate of the positive expression of bFGF in ICC was significantly higher than that in NCE 88.8% (127/143) vs. 25.0% (5/20, P = 0.000). The rate of positive expression of PTEN in ICC was significantly lower than that in NCE 67.1% (96/143) vs. 100.0% (20/20, P = 0.000). The expression of bFGF was positively correlated with lymph node metastasis and histopathological grading (r = 0.239, P = 0.004 and r = 0.369, P = 0.000, respectively). The expression of PTEN was negatively correlated with FIGO staging, histopathological grading and lymph node metastasis (r = -0.189, P = 0.024; r = -0.211, P = 0.011; r = -0.321, P = 0.000, respectively). The expression of bFGF was negatively correlated with the expression of PTEN in ICC (r = -0.261, P = 0.002). The overexpression of bFGF and underexpression of PTEN are closely related to the invasion and growth of cervical carcinoma. Detection of the expression of both bFGF and PTEN may be of value in further understanding the biological behavior and predicting the prognosis of cervical carcinoma.
Wei M.,The Third Hospital of Wuhan
Medical Journal of Wuhan University | Year: 2015
Objective: To study the predictive value of combined detection of C-reactive protein (CRP) and fibrinogen (FIB) for prevalence of cardiovascular disease in angina pectoris (AP) patients. Methods: 100 coronary heart disease (CHD) patients were selected and divided into SAP group and UAP group according to the types of AP, with 50 cases of each group. In addition, 50 healthy check-ups were selected as control group. The levels of CRP and FIB were separately detected. After 3 months of clinical follow-up visits, the prevalence of cardiovascular events for SAP and UAP groups were observed. Results: The levels of CRP and FIB of SAP group were (3.10±0.53) mg/L and (3.45±0.37) g/L; the levels of CRP and FIB of UAP groups were (5.63±0.76) mg/L and (4.30±0.43) g/L; the levels of CRP and FIB of control group were (2.55±0.35) mg/L and (2.63±0.20) g/L; the levels of CRP and FIB of SAP and UAP group were significantly higher than that of control group (P<0.05); the levels of CRP and FIB of UAP group were significantly higher than that of SAP group (P<0.05); within 3 months, the incidence rate of cardiovascular event of UAP group (81.08%) was significantly higher than that of SAP group (21.21%) (P<0.05); for patients with higher CRP and FIB levels, the incidence rate of cardiovascular event within 3 months was significantly higher than that of patients with normal CRP and FIB levels. Conclusion: The levels of CRP and FIB are closely correlated with the severity in CHD AP patients. The combined detection of CRP and FIB can predict the prevalence of cardiovascular events. ©, 2015, Editorial Board of Medical Journal of Wuhan University. All right reserved.