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Ren R.,University of Sichuan | Huang G.,the Third Hospital of Mianyang | Zhang J.,Chinese University of Hong Kong | Zhou J.,University of Sichuan | And 8 more authors.
Sleep and Breathing | Year: 2016

Purpose: The aim of this study was to investigate gender differences of periodic limb movements during sleep (PLMS) in patients with obstructive sleep apnea (OSA). Methods: This was a case–control study recruiting 364 patients with OSA (182 men, 182 women) matched for age and apnea–hypopnea index (AHI). All participants underwent overnight polysomnography (PSG), followed by the multiple sleep latency test (MSLT) and the Epworth Sleepiness Scale (ESS). Results: Women with OSA had a significantly higher prevalence of PLMS than men (24.2 vs. 15.9 %, p < 0.05). Women with OSA showed an increased prevalence of PLMS compared to men in the younger group aged ≤55 years (23.0 vs. 10.6 %, p < 0.05), but not in the older groups >55 years (25.3 vs. 21.6 %, p > 0.05). Binary linear regression analysis in OSA patients confirmed that women were more likely to have PLMS than men (OR 1.71, 95 % CI 1.00–2.92), particularly in patients with age ≤55 years old (OR 2.48, 95 % CI 1.06–5.79), after adjusting for age, BMI, AHI, and habits of smoking and drinking. Conclusions: The results demonstrate that, for patients with OSA, young women had significantly increased prevalence of PLMS compared to young men, but there was no difference in prevalence of PLMS between the men and women in the older age group. © 2015, Springer-Verlag Berlin Heidelberg.

Chen Q.,University of Sichuan | Peng X.D.,The Second Hospital of Sichuan | Huang C.Q.,the Third Hospital of Mianyang | Hu X.Y.,University of Sichuan | Zhang X.M.,University of Sichuan
Genetics and Molecular Research | Year: 2015

In the present study, we examined whether the ARNTL (BMAL1) rs2278749 T/C polymorphism was associated with the susceptibility to Alzheimer disease (AD). This case-control study examined the genotypes of apolipoprotein E (APOE e4) and BMAL1 rs2278749 T/C using restriction fragment length polymorphism and the TaqMan assay, respectively. A total of 296 unrelated AD patients and 423 control subjects were included. Both in the entire sample and in APOE e4 non-carriers, the prevalence of T carriers in BMAL1 rs2278749 T/C in AD patients was significantly higher than that in control subjects (entire sample: χ2 = 12.950, P < 0.0001; APOE e4 non-carriers: χ2 = 13.094, P < 0.0001). Both in the entire sample and in APOE e4 non-carriers, the prevalence of TT genotypes 2278749 in AD patients was also significantly higher than that in control subjects (entire sample: χ2 = 7.765, P = 0.024; APOE e4 non-carriers: χ2 = 13.062, P < 0.0001). However, among APOE e4 carriers, the difference in the prevalence of T carriers or TT genotypes in the BMAL1 rs2278749 T/C between patients and control subjects presents was not significant (T carriers: χ2 = 0.078, P = 0.851 or TT genotypes: χ2 = 2.576, P = 0.325). Among APOE e4 non-carriers, T carriers in the BMAL1 rs2278749 T/C were associated with a high susceptibility to AD, but among APOE e4 carriers, the association between AD and BMAL1 rs2278749 T/C was not significant. © FUNPEC-RP.

Xiong T.,Chongqing Medical University | Tang W.,Chongqing Medical University | Liu S.-X.,Chongqing Medical University | He Y.-F.,Chongqing Medical University | And 2 more authors.
Journal of Reproduction and Contraception | Year: 2010

Objective: To prove that juxtacrine and paracrine signaling are essential in the culture of spermatogonial stem cells (SSCs) with Sertoli cell feeder layer in vitro. Methods: Mice aged 7 d were chosen to harvest testes. A two-step enzyme digestion method was applied in testis suspension. The SSCs and Sertoli cells were separated by adherence distinguishing methods and biologically identified by immunofluorescence and Oil Red O staining methods. Flow cytometry was used to analyze purity of SSCs. Three groups were constructed according to different culture conditions: SSC and Sertoli cell co-culture group, SSC conditional culture group and SSC routine culture group. The conditional medium was collected from supernate of culture Sertoli cell in vitro and double-concentrated with DMEM/F12 and fetal bovine serum in a proportion of 4.5: 4.5: 1. The routine medium was DMEM/F12 containing 10% fetal bovine serum. Adherence rates were measured by Trypan blue staining. Absorbance of SSCs of each group was measured by MTT assay and proliferation curves shown to demonstrate proliferative features of SSCs. Proliferative features and colony formation were observed by inverted microscope. With 24 h difference in adherence rates, proliferations were compared and analyzed. Results: The adherence rate of co-culture group was greater than that in the others (P<0.05), with insignificant difference in conditional culture group and routine group (P>0.05). SSCs of co-culture group showed stable proliferation immediately following inoculation. A stable colony formed within 7-10 d and maintained for 30 d. SSCs in conditional culture group and routine group decreased rapidly following transient proliferation. Conclusion: The actions of SSCs in Sertoli cell cultures in vitro depended on both juxtacrine and paracrine signaling, Sertoli cell paracrine signaling was unable to promote SSC adherence and proliferation alone. © 2010 The Editorial Board of Journal of Reproduction and Contraception.

Yan X.-Y.,University of Sichuan | Huang S.-M.,University of Sichuan | Huang C.-Q.,the Third Hospital of Mianyang | Wu W.-H.,University of Sichuan | Qin Y.,Chongqing Medical University
Journal of International Medical Research | Year: 2011

This study assessed the relationship between marital status and the risk for depression in people ≥ 55 years old. Using the Medline™, EMBASE™ and Cochrane Library databases, clinical studies that published data on the association between marital status and risk of depression among individuals aged ≥ 55 years were identified. A quantitative meta-analysis of 24 cross-sectional and eight longitudinal studies was performed. Compared with married elderly people, unmarried elderly people had a higher risk for depression (odds ratio [OR], 1.55; relative risk [RR], 1.36). Compared with married elderly people, the widowed elderly people (OR 1.49; RR 1.71), divorced people (RR 2.14) and never-married people (OR 1.32) had a higher risk for depression. Among elderly unmarried people, widowed people had a higher risk for depression than those who never married (OR 1.51). In conclusion, being unmarried was an important risk factor for depression in elderly people. © 2011 Field House Publishing LLP. © 2011 Field House Publishing LLP.

Xiong B.,Peking University | Ma L.,the Third Hospital of Mianyang | Huang W.,The Ninth Peoples Hospital of Chongqing City | Cheng Y.,Chongqing Medical University | And 2 more authors.
Surgical Oncology | Year: 2014

Background: Patients with locally advanced gastric cancer (AGC) have a poor outcome. However, the efficacy of neoadjuvant chemotherapy (NAC) for improvement of survival and prognosis is still controversial and no definite conclusion has been drawn from randomized controlled trials (RCTs). We performed an updated meta-analysis to assess the efficacy and safety of NAC in the treatment of AGC. Methods: A systematic literature search was carried out using PubMed, Cochrane Library, EMBASE and ASCO proceedings up to June 2012. All eligible RCTs comparing the effect of NAC combined surgery versus surgery alone in gastric cancer were included. Results: A total of 12 RCTs comparing NAC (n=879) with surgery alone (n=941) published from 1993 to 2011 were identified. NAC can slightly improve the survival rate [OR=1.32, 95% confidence interval (CI): 1.07-1.64, P=0.01]. It can significantly improved the 3-year disease-free survival (DFS) [OR: 1.85, (1.39, 2.46), P <0.0001], tumor down-staging rate [OR: 1.71, (1.26, 2.33), P=0.0006] and R0 resection rate [OR: 1.38, (1.08, 1.78) P=0.01] of patients with AGC. Grade 3/4 adverse effects, relapse rates, operative complications, and perioperative mortality were similar between the two groups. Conclusions: NAC is safe and marginally effective. It requires further prospective multinational multicenter RCTs to define the clinical benefits of NAC combined with surgery and the most effective strategies for AGC. © 2014 Elsevier Ltd.

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